30 results about "Single infection" patented technology

The invention relates to the field of molecular biology and production of animal medicine, in particular to a multi-PCR (Polymerase Chain Reaction) specific primer for detecting bacterial enteritis pathogens, such as escherichia coli, salmonellae and clostridium perfringens, of a pig; nucleotide sequences are shown as SEQ ID NO:1 to SEQ ID NO:6; the specificity, the repeatability and the sensitivity are high. The invention also discloses a method which is established on the basis of the primer and is used for detecting the bacterial enteritis pathogens, such as the escherichia coli, the salmonellae and the clostridium perfringens, of the pig; the enteritis of the pig which is caused by single infection or mixed infection of the escherichia coli, the salmonellae and the clostridium perfringens can be distinguished quickly; the primer can be used to identify and diagnose quickly and effectively, and is beneficial to formulating targeted prevention and control measures to perform immune prevention and control on the bacterial enteritis pathogens of the pig in clinical parturition and ensure smooth parturition of the raised pig.

The invention aims at fully utilizing the advantages of a Taqman probe technology, designing specific probes against various genotypes of HBV (hepatitis B virus), using the probes to mark different fluorescent dyes, detecting at different wavelengths and further achieving the genotyping purpose. A kit disclosed by the invention can detect B, C and D type hepatitis B virus in samples and detect B, C and D type single infection and mixed infection by designing primers and the specific probes against all the genotypes of the HBV. The B (or D) type hepatitis B virus is detected by adopting a double-color probe at FAM wavelength and the C-type hepatitis B virus is detected at HEX wavelength. The kit has the advantages that the B, C and D type hepatitis B virus can be simultaneously detected in one experiment, the operation is simple, dUTP and UNG enzymes are used in the kit, and the interference of a polluted amplified product on a detection result can be effectively eliminated.

The present invention provides a method and device for calculating the relationship between high-risk HPV types and the stage of cervical precancerous lesions. The data of N types of high-risk HPV infection in the stage of precancerous lesions are classified and sorted, and the pre-processing data of HPV infection under different infection modes are obtained; cluster analysis is performed based on the HPV pre-processing data, and different high-risk HPV types are obtained based on the cluster analysis results Based on the HPV pretreatment data under the single infection and multiple infection mode, the Poisson distribution modeling is carried out, and regression analysis is carried out to obtain the impact ratio of HPV single infection and multiple infection on cervical precancerous lesions. The method combines clustering technology and statistical analysis method to mine biological data, and discovers the relationship between different high-risk HPV types and different stages of cervical precancerous lesions.

The invention discloses a test strip used in one-step rapid detection of reticuendotheliosis virus (REV) and subgroup-J avian leukosis virus (ALV-J). The test strip comprises a non-absorbing supporting layer, and an absorption layer adhered to the supporting layer. The absorption layer is formed by sequentially spliced components of an absorption fiber layer, a gold-labeled antibody fiber layer, a cellulose film layer, and a water absorption layer. The cellulose film layer is marked with anti-goat IgG or anti-mouse IgG control blot, and a detection blot comprising anti-REV and anti-ALV-J antibody. The anti-REV and anti-ALV-J antibody is an anti-REV and anti-ALV-J antibody monoclonal or polyclonal antibody. An anti-REV and anti-ALV-J mixed polyclonal antibody or monoclonal antibody marked by colloidal gold and corresponding to the detection blot is adhered to the gold-labeled antibody fiber layer. The test strip provided by the invention has the advantages of high detection specificity, high sensitivity, simple operation, fast detection, and intuitive result. The test strip is suitable for REV and ALV-J virus on-site rapid combined detection, and can be used in identification and diagnosis of single infection or mixed infection of the two viruses. The test strip can be widely applied, and is suitable for popularization.

The invention discloses a test strip used in one-step rapid detection of Marek's disease virus (MDV) and subgroup-J avian leukosis virus (ALV-J). The test strip comprises a non-absorbing supporting layer, and an absorption layer adhered to the supporting layer. The absorption layer is formed by sequentially spliced components of an absorption fiber layer, a gold-labeled antibody fiber layer, a cellulose film layer, and a water absorption layer. The cellulose film layer is marked with anti-goat IgG or anti-mouse IgG control blot, and a detection blot comprising anti-MDV and anti-ALV-J antibody. The anti-MDV and anti-ALV-J antibody is an anti-MDV and anti-ALV-J antibody monoclonal or polyclonal antibody. An anti-MDV and anti-ALV-J mixed polyclonal antibody or monoclonal antibody marked by colloidal gold and corresponding to the detection blot is adhered to the gold-labeled antibody fiber layer. The test strip provided by the invention has the advantages of high detection specificity, high sensitivity, simple operation, fast detection, and intuitive result. The test strip is suitable for MDV and ALV-J virus on-site rapid combined detection, and can be used in identification and diagnosis of single infection or mixed infection of the two viruses. The test strip can be widely applied, and is suitable for popularization.

Based on a highly conserved domain of a foot-and-mouth disease virus 3D protein coding gene, a vesicular stomatitis virus N protein coding gene and a swine vesicular disease virus VP1 protein coding gene, the invention designs a specific primer and a probe and develops a multiple fluorescence RT-PCR detection method used for simultaneously detecting the three animal viruses. The detection method can detect 102 copied plasmids containing target amplification sequences in 1 h. The method has high sensitivity and good specificity, and can achieve rapid high-throughput fluorescence RT-PCR detection for single-virus infection or mixed infection by a plurality of viruses.

The invention provides a vaccine composition, which contains immunological amount of SIV, Mhp, APP antigen and veterinary acceptable carrier. The immunization procedure of the vaccine composition is simple, and can effectively prevent and treat the single infection or mixed infection of SIV, Mhp and APP. When mixed infection occurs, the immune effect is significantly higher than that of single vaccine injections respectively. The vaccine composition has few side effects, long immunization period, less time-consuming and labor-intensive production process, low immunization cost and strong practicability.

The invention discloses a method for breeding summer truffle root seedling through inoculation of suspension liquid, which is characterized in that the difficulty in artificial inoculation of summer truffle can be solved through steps of breeding of aseptic seedling, preparation of microbial inoculum, inoculation of suspension liquid, seedling exercising, breeding of fungus seedlings and test and confirmation. Compared with the existing breeding method, the method is characterized in that pinus armandi, castanea mollissima and pinus yunnanensis are adopted as hosts; under a relatively aseptic condition, i.e. the condition for preventing the competition of fungi and infection of most mycetes, the summer truffle spores sufficiently contact with roots of the host trees; the single infection predominance of the summer truffle is maintained within a given period of time; and a great number of firm summer truffle mycorrhizas can be formed.

The invention provides an externally-applied drug for treating skin eczema and eczematous dermatitis and a preparation method thereof. The externally-applied drug is prepared from miconazole nitrate, chloramphenicol, urea, triamcinolone acetonide acetate, stearic acid, Vaseline, glycerol monostearate, glycerol, purified water, triethanolamine, lauryl sodium sulfate and ethylparaben. The externally-applied drug can effectively treat and prevent fungus and bacterium single infection and concurrent infection diseases. The externally-applied drug can effectively treat and prevent skin fungus and bacterium single infection and concurrent infection, eczema, eczematous dermatitis and other skin diseases, and the recovery process of various skin diseases is accelerated. Meanwhile, the skin layer rapidly and fully absorbs all the ingredients of the externally-applied drug, all the ingredients are in compatibility in corresponding proportions, the treatment effect of the externally-applied drug prepared through the preparation method is remarkably improved, and the externally-applied drug has the effects of relieving itching, diminishing inflammation, achieving sterilization and eliminating skin surface symptoms, and especially has the obvious effect on various kinds of fungal infection and severe infantile eczema.