6 results about "Single infection" patented technology

The application relates to the field of medical diagnosis, and particularly discloses a biomarker combination, a kit and application thereof for HIV / Mtb co-infection immune state evaluation and clinical diagnosis. Peripheral blood samples of HIV infectors, Mtb infectors and HIV / Mtb co-infectors are subjected to multi-factor detection, and Lasso regression is adopted to screen out cytokines such as FGF-2, IL-7, I-309, TNF-alpha, MMP-1 and MMP-7 closely related to HIV / Mtb co-infection, so as to construct a multi-factor diagnosis model. The model shows high sensitivity, specificity and AUC value in the training set and the test set, can effectively distinguish HIV single infection from HIV / Mtb co-infection individuals, and is particularly suitable for the diagnosis of patients with low CD4 + T cell level. The biomarker combination and the kit prepared by the biomarker combination provided by the application can be used as a supplement to immunological detection methods, and provide a new technical means for the clinical diagnosis and immune state evaluation of HIV / Mtb co-infection.

The invention discloses a dual real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for simultaneously detecting porcine hemagglutinated encephalomyelitis virus (PHEV) and porcine teschovirus (PTV), and belongs to the technical field of animal virus molecular biological detection. Aiming at the problems of similar clinical symptoms, high fatality rate and lack of synchronous detection and identification means after the suckling piglets are infected by PHEV and PTV, a specific primer pair and a probe are designed and screened through sequence comparison based on conserved regions of genomes of the two viruses. The detection method provided by the invention can realize qualitative and quantitative analysis of the two viruses, has the advantages of high sensitivity, high specificity and simplicity and convenience in operation, can quickly identify single infection or mixed infection of the two viruses, and provides an effective technical means for accurate detection in laboratories and epidemiological investigation.

The invention provides a multiplex PCR primer group and a reagent for simultaneously detecting three pathogenic genotypes of theileria orientalis, specific primers are designed on the basis of theileria orientalis MPSP genes, and a multiplex PCR method capable of simultaneously detecting Ikeda type, Chitose type and Buffeli type is established through primer combination screening and optimization of annealing temperature, primer concentration and Mg < 2 + > concentration. According to the primer group and the multiplex PCR method, mixed infection and single infection conditions of three common genotypes, namely the Ikeda type, the Chitose type and the Buffeli type, of theileria orientalis in a sample to be detected are determined through one-time multiplex PCR reaction, and compared with a common PCR method, more time is saved; compared with real-time fluorescent quantitative PCR, more cost is saved; and the method has the advantages of rapidness, economy and high efficiency, has low requirements on operators and equipment, is suitable for clinical detection, and provides technical support for diagnosis and prevention and control of different genotypes of theileria orientalis.

This invention relates to the field of parasite detection technology, specifically to a TaqMan-MGB multiplex quantitative PCR primer set, probe, kit, and method for detecting three pathogenic Eimeria species in goats. This invention provides a TaqMan-MGB multiplex quantitative PCR primer set and probe for detecting *Eimeria cruzi*, *Eimeria aurea*, and *Eimeria yabrilliosa*, and establishes a TaqMan-MGB multiplex quantitative PCR method for detecting these three species based on this primer set and probe. The primer set, probe, and TaqMan-MGB multiplex quantitative PCR method of this invention can determine the mixed or single infection status of *Eimeria cruzi*, *Eimeria aurea*, and *Eimeria yabrilliosa* in a single TaqMan-MGB multiplex quantitative PCR reaction, offering advantages such as better repeatability, higher sensitivity, stronger specificity, and simpler operation compared to conventional PCR and nano-PCR methods.

This invention discloses a method for constructing a dynamic pathogen identification network based on time-series images and clinical data, belonging to the field of artificial intelligence technology. The method uses a dual-channel deep encoder composed of a 3D convolutional neural network and a recurrent neural network. After confidence-gated fusion, a fused feature vector is extracted. A multi-head cross-attention network with an inter-prototype contrast modulation mechanism is introduced to decompose the fused feature vector into a sparse superposition of category-specific components, achieving deep category attribution analysis of the mixed signal. A second network jointly analyzes the decomposition residuals and dimensional competition features. When the residual significance score exceeds a first preset threshold, a feedback signal is injected into the first network for a second round of conditional refinement decomposition, forming a deep learning dynamic network of decomposition-evaluation-guidance-re-decomposition. This invention can adaptively identify three scenarios: single infection, mixed infection, and weak signal infection.

The invention discloses a preparation method and an application of a porcine reproductive and respiratory syndrome (PRRS) replication-deficient vaccine rPRRSV-ORF5a strain. The PRRSV replication-deficient vaccine rPRRSV-ORF5a strain disclosed by the invention is normally replicated in a Marc-145 cell line for stably expressing the PRRSV ORF5a protein and has relatively high virus titer, and only one-time infection can be carried out in a common Marc-145 cell line. Animal experiments prove that the replication-deficient virus rPRRSV-ORF5a strain can resist infection of homologous virulent viruses and has an immune protection effect. The vaccine strain is expected to become a safe and effective novel PRRSV vaccine, effectively overcomes the defects that a PRRSV inactivated vaccine cannot induce cellular immunity and the like, and also solves the safety problem of a PRRSV attenuated vaccine.