13 results about "Single infection" patented technology

The invention discloses a primer group for identifying six different genotypes of IBV epidemic strains and a high-resolution melting curve detection method, and relates to the technical field of virus detection. The primer group for identifying the six IBV epidemic strains with different genotypes is HRM-F / HRM-R, and the nucleotide sequences of the primer group are shown as SEQ ID NO. 1 and SEQ ID NO. 2. By utilizing the specific primer group and the detection method disclosed by the invention, qualitative and quantitative analysis on single infection or mixed infection of IBV epidemic strain genotypes of LX4 (GI-19), Mass (GI-I), 4 / 91 (GI-13), LDT3-A (GI-28), Taiwan (GI-7) and CK / CH / LSC / 99I (GI-22) can be realized. The detection sensitivity is high, the specificity is good, the accuracy is high, the operation is simple and rapid, the cost is low, and the method is suitable for popularization.

The present invention discloses a dual nano-PCR detection kit for porcine reproductive and respiratory syndrome virus and pseudorabies virus and its application, belonging to the technical field of molecular biology. The dual nano-PCR detection kit for porcine reproductive and respiratory syndrome virus and pseudorabies virus includes a primer set for detecting porcine reproductive and respiratory syndrome virus (PRRSV) and a primer set for detecting pseudorabies virus (PRV). The primers of the kit of the present invention have strong specificity, good repeatability and high sensitivity. Using nano-dual PCR for detection, it can detect as low as 5.17×10<supgt;2< / supgt> copies / μL and 4.46×10<supgt;2< / supgt> copies / μL, which is 100 times more sensitive than ordinary PCR; a single detection operation can simultaneously identify single infection or co-infection of the two viruses, greatly saving the detection time and reducing the detection cost, and providing technical support for disease prevention and control in pig farms.

The invention relates to the technical field of intestinal pathogen detection, in particular to a multiplex PCR (polymerase chain reaction) primer group, a kit and a method for detecting pathogens causing calf diarrhea. The invention provides a primer group for detecting diarrheagenic Escherichia coli eaeA, stx1 and stx2 virulence genes, clostridium perfringens and cryptosporidium, and a multiple PCR (Polymerase Chain Reaction) method for detecting the diarrheagenic Escherichia coli eaeA, stx1 and stx2 virulence genes, clostridium perfringens and cryptosporidium is established on the basis of the primer group. According to the primer group and the multiple PCR method disclosed by the invention, the mixed infection and single infection conditions of diarrheogenic Escherichia coli eaeA, stx1 and stx2 virulence genes, clostridium perfringens and cryptosporidium in a sample to be detected can be determined through one-time multiple PCR reaction, and compared with a common PCR method, the primer group and the multiple PCR method are more convenient and quicker, and time and resources are saved; and compared with real-time fluorescent quantitative PCR, the method is more cost-saving and convenient to operate.

The application relates to the field of medical diagnosis, and particularly discloses a biomarker combination, a kit and application thereof for HIV / Mtb co-infection immune state evaluation and clinical diagnosis. Peripheral blood samples of HIV infectors, Mtb infectors and HIV / Mtb co-infectors are subjected to multi-factor detection, and Lasso regression is adopted to screen out cytokines such as FGF-2, IL-7, I-309, TNF-alpha, MMP-1 and MMP-7 closely related to HIV / Mtb co-infection, so as to construct a multi-factor diagnosis model. The model shows high sensitivity, specificity and AUC value in the training set and the test set, can effectively distinguish HIV single infection from HIV / Mtb co-infection individuals, and is particularly suitable for the diagnosis of patients with low CD4 + T cell level. The biomarker combination and the kit prepared by the biomarker combination provided by the application can be used as a supplement to immunological detection methods, and provide a new technical means for the clinical diagnosis and immune state evaluation of HIV / Mtb co-infection.

The invention belongs to the field of biological information, particularly relates to a construction method for predicting a lesion model based on an HPV infection rate, and more particularly relates to a construction method, device and equipment for predicting a lesion model based on a single infection rate and a co-infection rate of an HPV. The invention provides a construction method for predicting a lesion model based on an HPV infection rate, and the method comprises the steps: obtaining training data, which comprises an HPV typing detection result and a detection result; determining a single infection rate and a co-infection rate according to an HPV typing detection result; according to the HPV typing detection result and the detection result, determining the relationship between the single infection rate and the detection result as well as between the co-infection rate and the detection result; and constructing a model for predicting lesion based on the HPV infection rate according to the relationship with the detection result. By means of the model building method, a prediction model can be built, and lesions such as TCT detection, colposcope detection results and cervical biopsy results can be predicted through the single infection rate and the co-infection rate.

The invention discloses a dual real-time fluorescent quantitative PCR (polymerase chain reaction) detection method for simultaneously detecting porcine hemagglutinated encephalomyelitis virus (PHEV) and porcine teschovirus (PTV), and belongs to the technical field of animal virus molecular biological detection. Aiming at the problems of similar clinical symptoms, high fatality rate and lack of synchronous detection and identification means after the suckling piglets are infected by PHEV and PTV, a specific primer pair and a probe are designed and screened through sequence comparison based on conserved regions of genomes of the two viruses. The detection method provided by the invention can realize qualitative and quantitative analysis of the two viruses, has the advantages of high sensitivity, high specificity and simplicity and convenience in operation, can quickly identify single infection or mixed infection of the two viruses, and provides an effective technical means for accurate detection in laboratories and epidemiological investigation.

The invention provides a multiplex PCR primer group and a reagent for simultaneously detecting three pathogenic genotypes of theileria orientalis, specific primers are designed on the basis of theileria orientalis MPSP genes, and a multiplex PCR method capable of simultaneously detecting Ikeda type, Chitose type and Buffeli type is established through primer combination screening and optimization of annealing temperature, primer concentration and Mg < 2 + > concentration. According to the primer group and the multiplex PCR method, mixed infection and single infection conditions of three common genotypes, namely the Ikeda type, the Chitose type and the Buffeli type, of theileria orientalis in a sample to be detected are determined through one-time multiplex PCR reaction, and compared with a common PCR method, more time is saved; compared with real-time fluorescent quantitative PCR, more cost is saved; and the method has the advantages of rapidness, economy and high efficiency, has low requirements on operators and equipment, is suitable for clinical detection, and provides technical support for diagnosis and prevention and control of different genotypes of theileria orientalis.

This invention relates to the field of parasite detection technology, specifically to a TaqMan-MGB multiplex quantitative PCR primer set, probe, kit, and method for detecting three pathogenic Eimeria species in goats. This invention provides a TaqMan-MGB multiplex quantitative PCR primer set and probe for detecting *Eimeria cruzi*, *Eimeria aurea*, and *Eimeria yabrilliosa*, and establishes a TaqMan-MGB multiplex quantitative PCR method for detecting these three species based on this primer set and probe. The primer set, probe, and TaqMan-MGB multiplex quantitative PCR method of this invention can determine the mixed or single infection status of *Eimeria cruzi*, *Eimeria aurea*, and *Eimeria yabrilliosa* in a single TaqMan-MGB multiplex quantitative PCR reaction, offering advantages such as better repeatability, higher sensitivity, stronger specificity, and simpler operation compared to conventional PCR and nano-PCR methods.

The invention belongs to the field of veterinary drugs, and particularly relates to a Tibetan veterinary drug composition and application of the Tibetan veterinary drug composition in treatment of infectious diarrhea of yaks. The composition comprises nine types of Tibetan veterinary medicinal plants including Geranium tanguticum, Swertia speckled, delphinium, dracocephalum tanguticum, safflower, Sowthistle, trogopterus dung or charred Wuling, licorice, Forsythia suspensa leaf and Scutellaria baicalensis. The Tibetan veterinary drug composition contains antibacterial, anti-inflammatory, antiviral, antiprotozoal and other active components, and shows relatively strong corrosion resistance besides the pharmacological activity, so that the addition of excessive preservatives is avoided, the relatively long storage time is ensured, and the Tibetan veterinary drug composition has dual effects. The medicine components are administered in an oral liquid form to form a safe, efficient, durable and convenient Tibetan veterinary medicine composition, and the Tibetan veterinary medicine composition can be used for treating diarrhea diseases of yaks of different age groups caused by single infection and mixed infection of bacteria, viruses and parasites.

The invention belongs to the technical field of vaccine preparation, and particularly relates to an attenuated vaccine resistant to tomato chlorosis virus and tomato yellow leaf curl virus as well as a construction method and application of the attenuated vaccine. The attenuated vaccine comprises a tomato chlorosis virus resistant mutant and a tomato yellow leaf curl virus resistant mutant, the mutant resistant to the tomato chlorotic virus comprises a gene segment which is any one of gene segments as shown in SEQ ID NO.1-5. The mutant resistant to the tomato chlorotic virus is a mutant resistant to the tomato chlorotic virus. The mutant resistant to the tomato yellow leaf curl virus comprises a gene segment as shown in SEQ ID NO. 6 or SEQ ID NO. 7. The vaccine prepared according to the method disclosed by the invention not only can resist skeleton vector viruses, but also can resist tomato chlorosis viruses and tomato yellow leaf curl viruses, and provides technical support for prevention and treatment of single infection and composite infection of ToCV and TYLCV.

This invention discloses a method for constructing a dynamic pathogen identification network based on time-series images and clinical data, belonging to the field of artificial intelligence technology. The method uses a dual-channel deep encoder composed of a 3D convolutional neural network and a recurrent neural network. After confidence-gated fusion, a fused feature vector is extracted. A multi-head cross-attention network with an inter-prototype contrast modulation mechanism is introduced to decompose the fused feature vector into a sparse superposition of category-specific components, achieving deep category attribution analysis of the mixed signal. A second network jointly analyzes the decomposition residuals and dimensional competition features. When the residual significance score exceeds a first preset threshold, a feedback signal is injected into the first network for a second round of conditional refinement decomposition, forming a deep learning dynamic network of decomposition-evaluation-guidance-re-decomposition. This invention can adaptively identify three scenarios: single infection, mixed infection, and weak signal infection.

The invention discloses a preparation method and an application of a porcine reproductive and respiratory syndrome (PRRS) replication-deficient vaccine rPRRSV-ORF5a strain. The PRRSV replication-deficient vaccine rPRRSV-ORF5a strain disclosed by the invention is normally replicated in a Marc-145 cell line for stably expressing the PRRSV ORF5a protein and has relatively high virus titer, and only one-time infection can be carried out in a common Marc-145 cell line. Animal experiments prove that the replication-deficient virus rPRRSV-ORF5a strain can resist infection of homologous virulent viruses and has an immune protection effect. The vaccine strain is expected to become a safe and effective novel PRRSV vaccine, effectively overcomes the defects that a PRRSV inactivated vaccine cannot induce cellular immunity and the like, and also solves the safety problem of a PRRSV attenuated vaccine.

The invention relates to the technical field of parasite detection, in particular to a TaqMan-MGB multiple fluorescent quantitative PCR (polymerase chain reaction) primer group, a probe, a kit and a method for detecting three pathogenic eimeria of goats. The invention relates to a TaqMan-MGB multiplex fluorescent quantitative PCR (Polymerase Chain Reaction) primer group and a probe for detecting Eimeria klebsii, Eimeria adamsii and Eimeria elegans, and a TaqMan-MGB multiplex fluorescent quantitative PCR method for detecting the Eimeria klebsii, the Eimeria adamsii and the Eimeria elegans is established on the basis of the primer group and the probe, so that the TaqMan-MGB multiplex fluorescent quantitative PCR primer group and the probe can be used for detecting the Eimeria klebsii, the Eimeria adamsii and the Eimeria elegans, the Eimeria adamsii and the Eimeria elegans. According to the primer group, the probe and the TaqMan-MGB multiple fluorescent quantitative PCR method disclosed by the invention, the mixed infection and single infection conditions of the Eimeria kirsuki, the Eimeria adamsii and the Eimeria delbrueckii in a clinical sample to be detected can be determined through one TaqMan-MGB multiple fluorescent quantitative PCR reaction; compared with common PCR and nano PCR methods, the method has the advantages of good repeatability, high sensitivity, strong specificity, simple operation and the like.