3results about How to "Increase proliferation rate" patented technology

This invention discloses a culture medium and method for expanding adult human lung pluripotent stem cells. It relates to the field of primary lung stem cell culture technology, including specific components, culture methods based on the culture medium, and applications. The culture medium of this invention can continuously and stably culture adult human lung stem cells for more than 30 generations. This invention can stably expand adult human lung pluripotent stem cells at the 2D level, and the stem cells maintain a high proliferation rate and multi-cell differentiation potential of lung epithelial cells after continuous passage. Importantly, after in vivo transplantation of the expanded adult pluripotent stem cells, the cells can achieve efficient cell integration and functional alveolar structure reconstruction in fibrotic lung tissue, and the cell transplantation safety is high. This is of great significance for advancing stem cell therapy for respiratory diseases and research on the pathogenic mechanisms of respiratory diseases, and also provides excellent seed cells for subsequent construction of functional human lung organoids and screening of drugs for respiratory diseases represented by pulmonary fibrosis.

This disclosure belongs to the field of cell culture technology, and specifically relates to a serum-free culture medium for MSCs and its preparation method. The culture medium contains a fixed ratio of basic nutrients and six key regulatory factors, namely transferrin, ascorbic acid, human serum albumin, human fibroblast growth factor rhFGF, platelet-derived growth factor PDGF-BB, and transforming growth factor TGF-β1. By optimizing the combination and concentration of these six key regulatory factors, the proliferation rate and activity of MSCs are significantly improved. It also provides four suitable formulations: high proliferation, high activity, balanced, and economical. The culture effect is stable and reproducible, with high safety and a simple process, making it suitable for laboratory research and large-scale clinical-grade MSC culture.

The invention relates to the technical field of improved breeding of animal husbandry, and particularly discloses an improved NK cell culture medium and a preparation method thereof. The improved NK cell culture medium comprises a basic culture medium, a supplement and a composite sustained-release body. All the components are mixed according to a specific proportion, and the requirements of NK cell proliferation and function maintenance are met. Wherein the inner layer of the composite sustained-release body takes trehalose and human recombinant serum albumin as protective agents to wrap the interleukin, and the outer layer of the composite sustained-release body is an acrylate prepolymer photocuring polymer containing a photoinitiator, so that the continuous and stable release of the interleukin is realized. Through component optimization and slow release system design, the NK cell culture efficiency and activity are effectively improved, and the method is suitable for NK cell multiplication culture.