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Method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine

A dual inactivated vaccine and parvovirus technology, which is applied in the field of vaccines, can solve difficult problems and achieve the effects of improving stability, ensuring immunogenicity, improving immune effect and production performance

Inactive Publication Date: 2018-02-02
TIANJIN RINGPU BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention aims at the technical defects of the prior art, and provides a preparation method of porcine erysipelas and porcine parvovirus dual inactivated vaccine, so as to solve the problem that it is difficult for the vaccine products of the prior art to achieve immunity to the above two pathogens at the same time through one vaccination. technical problem

Method used

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  • Method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine
  • Method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine
  • Method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine

Examples

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Effect test

Embodiment 1

[0034] A preparation method of porcine erysipelas and porcine parvovirus dual inactivated vaccine, the steps are as follows:

[0035] 1 Production Seed Preparation

[0036] 1.1 Erysipelas suis production seed preparation

[0037] 1.1.1 Primary seed propagation

[0038] After unsealing the Erysipelas suis freeze-dried strains, add Martin broth to dilute and streak inoculate on the blood agar slope, culture at 37°C for 24 hours, streak on 10% serum Martin agar plate, culture for 24-36 hours, select a typical uniform Several bacterial colonies were inoculated on the slant of blood agar, cultured at 37°C for 24 hours, and used as primary seeds.

[0039] 1.1.2 Secondary seed propagation

[0040] Take the first-grade seeds and inoculate them in Martin Broth, incubate them at 37°C for 24 hours, then inoculate them in a large amount of meat liver and stomach digestion soup or Martin Broth, and incubate them for 24 hours, conduct a pure inspection according to the appendix of the cu...

Embodiment 3

[0070] Example 3 Vaccine Efficacy Research

[0071] 1 Experimental design

[0072] 1.1 Vaccine Immunization

[0073] Get the vaccine prepared in Example 1, mark the portion by the bottle label, each portion is 2mL, intramuscularly inject 1 portion into 10 healthy susceptible pigs with a body weight of more than 20kg 1-2 months after weaning, the first 21 days after the first immunization The dose and method of immunization were the same as that of immunization, and a control group of 10 animals was set up without immunization.

[0074] Purchase commercially available porcine erysipelas inactivated vaccine (C43-5) and porcine parvovirus inactivated vaccine (CP-99), and inject them into healthy and easy-to-care animals weighing more than 20kg 1-2 months after weaning according to the injection method and injection dosage according to their instructions respectively. Sensational pig.

[0075] Table 2 Vaccine immunization groups

[0076] Vaccine Grouping

Vaccine n...

Embodiment 4

[0087] A preparation method of porcine erysipelas and porcine parvovirus dual inactivated vaccine, comprising the following steps:

[0088] 1) Cultivate the Erysipelas suis type 2 C43-5 strain to prepare a live bacterial liquid;

[0089] 2) Adjust the antigen concentration in the live Erysipelas suis type 2 C43-5 liquid obtained in step 1) to 10 by diluting or centrifuging. 9 CFU / mL;

[0090] 3) Utilizing formaldehyde to inactivate the Erysipelas suis type 2 C43-5 bacterium liquid after step 2) adjusting the concentration;

[0091] 4) Breeding porcine parvovirus BJ-2 strain to prepare live venom;

[0092] 5) using formaldehyde to inactivate the porcine parvovirus BJ-2 strain venom in step 4);

[0093] 6) Mix the inactivated Erysipelas suis type 2 C43-5 bacterial liquid in step 3) and the inactivated parvovirus BJ-2 strain venom in step 5) at a volume ratio of 1:2 to obtain the mixed antigen solution;

[0094] 7) Mix the mixed antigen solution obtained in step 6) with an a...

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Abstract

The invention provides a method for preparing swine erysipelas and porcine parvovirus bivalent inactivated vaccine. The method has the advantages that bacteria solution is prepared from bacillus rhusiopathiae suis in fermentation modes in preparation procedures, a pH (potential of hydrogen) value of fermentation broth is strictly controlled in bacteria solution fermentation procedures, accordingly, the immunogenicity of bacillus rhusiopathiae suis antigens can be effectively guaranteed, and the protection rate can be higher than 90% without excessively high quantities of thalli of the bacillusrhusiopathiae suis; viruses can be reproduced from porcine parvovirus in IBRS-2 cell suspension culture modes by the aid of bioreactors in swine erysipelas and porcine parvovirus bivalent inactivatedvaccine preparation procedures, and accordingly the titer of virus liquid can be obviously improved; dissolved oxygen, pH, rotational speeds and the like are set in virus reproduction procedures, accordingly, the homogeneity of inter-assay virus liquid can be guaranteed, the shortcomings of complicated operation, high labor intensity, vulnerability to pollution and the like in spinner-bottle culture procedures can be overcome, and the quality stability of porcine parvovirus inactivated vaccine can be effectively enhanced.

Description

technical field [0001] The invention relates to the technical field of vaccines, and further relates to the preparation technology of multiple vaccines, in particular to a preparation method of a dual inactivated vaccine of porcine erysipelas and porcine parvovirus. Background technique [0002] Swine erysipelas (SE) is an acute, febrile infectious disease caused by Brysipelothrix rhusiopathiae, also known as red fever, also known as "fire mark". Clinically, high fever, rash and arthritis are the main features. my country classifies the disease as a second-class disease. After the disease has been quiet for 30 years, sporadic or large-scale outbreaks have appeared in recent years. [0003] Pigs are most sensitive to this bacteria. Sick pigs and pigs with bacteria are the main source of infection, and the digestive tract is the main transmission route. The bacteria can infect humans through skin wounds, which is called "like erysipelas". The disease mainly infects pigs age...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/23A61K39/295A61P31/04A61P31/20
Inventor 厚华艳吕茂杰范芳芳付旭彬郁宏伟梁武李守军
Owner TIANJIN RINGPU BIO TECH
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