A-type clostridium perfringens populus skin lipoid inactivated vaccine and preparation method thereof

A technology of Clostridium perfringens and poplar bark lipid, which is applied in the field of vaccines, can solve the problems of heavy vaccination reaction of livestock, short duration of immunity, strong toxic and side effects, etc., and achieves prolonged release of antigens and convenient storage and transportation , no mind-affecting effect

Inactive Publication Date: 2010-04-21
王靖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are still some problems that need to be further solved in the use of the developed vaccines: 1. The vaccination reaction of livestock is relatively severe; 2. The injection dose is large, and it is inconvenient to use; 3. The duration of immunity is short
3) Small irritation and no side effects: traditional vaccines use mineral oil as an adjuvant to prepare vaccines, which are highly irritating and have strong side effects. It is easy to take advantage of this period of time and cause disease outbreaks within 24 to 48 hours after immunization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Thaw Clostridium perfringens type A T5-A, aseptically inoculate in the anaerobic meat liver and stomach enzyme digestion soup that was boiled in a water bath for 10 minutes to remove oxygen, and culture anaerobically at 37°C for 15 hours. Bacteria were inoculated on 5% fresh blood-glucose-agar plates and anaerobic liver and stomach enzyme digestion soup respectively, and cultured to observe whether the culture characteristics and colony morphology were consistent. Then catch a few bacteria characteristic of Clostridium perfringens colonies with clear double hemolytic rings on the blood-glucose-agar plate, make slides, perform Gram staining, microscopic examination, and observe and record the results. Typical colonies were inoculated on blood slant, anaerobically cultured at 37°C for 24 hours, and their biochemical characteristics were identified by biochemical tests (sugar fermentation test, indole test, H2S test, nitrate reduction test, litmus milk medium test, etc.). ...

Embodiment 2

[0036] Thaw Clostridium perfringens type A T5-A, aseptically inoculate it in the anaerobic meat liver and stomach enzyme digestion soup that was boiled in a water bath for 10 minutes to remove oxygen, and culture anaerobically at 37°C for 20 hours. Bacteria were inoculated on 5% fresh blood-glucose-agar plates and anaerobic liver and stomach enzyme digestion soup respectively, and cultured to observe whether the culture characteristics and colony morphology were consistent. Then catch a few bacteria characteristic of Clostridium perfringens colonies with clear double hemolytic rings on the blood-glucose-agar plate, make slides, perform Gram staining, microscopic examination, and observe and record the results. Typical colonies were inoculated on blood slant, anaerobically cultured at 37°C for 20 hours, and their biochemical characteristics were identified by biochemical tests (sugar fermentation test, indole test, H2S test, nitrate reduction test, litmus milk medium test, etc.)...

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PUM

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Abstract

The invention discloses an A-type clostridium perfringens populus skin lipoid inactivated vaccine which is synthesized by taking populus skin lipoid as an aduvant and mixing A-type clostridium perfringens toxin subjected to inactivation and detoxication and the populus skin lipoid aduvant. The concrete method comprises the following steps: aseptically inoculating A-type clostridium perfringens in liver and gastric enzyme digestion soup, anaerobically culturing at 37 DEG C for 15-20h, selecting typical colonies to be inoculated on a blood inclined plane, and anaerobically culturing at 37 DEG C for 20-24h; then inoculating in the liver and gastric enzyme digestion soup according to a proportion of 1 percent of the total weight of a culture medium, culturing at a constant temperature of 35 DEG C for 7-8h, centrifuging to obtain the toxin, adding formaldehyde which is 0.3-0.5 percent of the total weight of the toxin, and carrying out inactivation and detoxication at 37 DEG C for 5d; mixing the toxin subjected to inactivation and detoxication with the populus skin lipoid aduvant according to the weight ratio of 4:1 and stirring at high speed to synthesize the vaccine. The A-type clostridium perfringens populus skin lipoid inactivated vaccine has long immunity time, no vaccination reaction after being injected and small stress.

Description

technical field [0001] The invention relates to a vaccine, in particular to an inactivated vaccine of Clostridium perfringens type A. The invention also relates to the preparation method of the vaccine. Background technique [0002] Clostridium perfringens (Clostridium peffringens), also known as Clostridium welchii, was first isolated by the British Welchii and Nuttad from a blood vessel that produced air bubbles in a decayed human corpse, and was named Welchii named Clostridium welchii. The bacterium can grow rapidly in various common mediums, and the most prominent biochemical characteristic is the "burst fermentation" of litmus milk medium, which can be used for rapid diagnosis. The bacteria can form obvious capsules in vivo, without flagella, and cannot move. It often exists in the form of spores in nature, but the formation of spores is difficult. The resistance of spores is very strong, and 10% formalin can kill them in 10 minutes. This bacterium is widely distri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/08A61K39/39A61P31/04
Inventor 王靖
Owner 王靖
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