Cell preservation method

Abstract

The invention discloses a cell preservation method, which comprises a cell preservation bottle, a cell preservation solution and a cell preservation method. The cell preservation bottle is provided with a chamber A and a chamber B which are independent and closed, and the cell preservation liquid is composed of a liquid A and a liquid B which are respectively stored in the chamber A and the chamber B of the cell culture bottle. After a cytological specimen is sequentially treated by the solution A and the solution B, red blood cells in the specimen can be completely removed, meanwhile, the cells are fixed, and cell debris is dissolved in a buffer system. The cell smear or slice prepared by the cell preservation method is clear in cell morphological structure, has no red blood cell and protein precipitation background, and is easy to make pathological diagnosis.

Description

technical field [0001] The patent relates to the field of cell biology, and specifically relates to a cell preservation method, especially a fine needle aspiration cell preservation method. Background technique [0002] Fine needle aspiration (aspiration) cytology (FNA) is an important development direction of cytopathology. Through the method of fine needle aspiration of human cells, clinical diagnostic specimens are obtained, and cell smears, cell wax blocks and cell slices are prepared, and further Carry out immunohistochemical and molecular pathological tests to diagnose, type, detect therapeutic targets and prognostic indicators for diseases. [0003] FNA pathological diagnosis technology has many advantages such as minimally invasive, safe, and simple. Theoretically, the fine needle can reach any part of the human body to take samples for disease diagnosis, reducing the difficulty of sampling for disease diagnosis. However, this technology also has obvious limitations,...

AI Technical Summary

Problems solved by technology

Similar liquid-based cell preservation solutions are also used in the preservation of fine-needle aspiration cells. There are two main types: one is based on methanol and ethanol, and this type of preservation solution does not have the function of removing red blood cells; the other is based on methanol and ethanol. Add glacial acetic acid, a erythrocyte lysing reagent, to the alcohol fixative solution. Glacial acetic acid is a drastic way to remove erythrocytes, which can quickly remove erythrocytes in the specimen, but it also causes obvious damage to the diagnostic cells and changes their morphology and structure; at the same time , the lysed red blood cell fragments and the released hemoglobin will exist in the form of precipitates in the alcohol fixative, which cannot be removed during the film production process, and the presence of precipitates will block the observation of cell morphology under the microscope

In this way, the cell smear (section) prepared from cells preserved in this type of cell preservation solution makes it difficult to make a cytopathological diagnosis based on the cell morphology and its microstructure, and sometimes even makes a wrong diagnosis.

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