46results about How to "Clean background" patented technology

The invention relates to a strand substitute polymerase chain reaction, which is characterized in that a target gene chain to be detected is substituted by a probe containing reporter gene chain having a different sequence for indirect amplification, the probe sequence is complementary sequences selecting two small segments of conserved or specific sequences adjacent to target genes as double probes, different sequences of the reporter gene chain refers to a gene sequence which has minimum homology with the target gene chain or is in farther germ line, and the head and the tail of the reportergene chain are added with the given double-probe sequences to serve as linear band probe reporter genes. The target genes to be detected and the given head and tail probe sequences of the reporter genes are complementally hybridized so that the head and the tail of linear reporter genes are closed and linked, single-strand gaps of a hybrid are connected by ligase, and the target link is substituted by a reporter gene ring, namely a reverse PCR amplified reporter gene ring. By monitoring different sizes (at two ends) of reverse PCR amplification or the reporter genes with different codes, multi-target molecules can be indirectly indicated. More than one set of reporter genes can be alternately used.

The invention discloses a liquid-based cell sheet producing system which comprises a rack, a sheet producing platform fixed on the rack, a plurality of sheet producing plates fixed on the sheet producing platform, a moving device perpendicularly connected with a bearing frame of the rack, a mechanical arm perpendicularly and slidingly connected with the moving device, a cantilever perpendicularly and slidingly connected with the mechanical arm, a reagent bottle connected with the cantilever by a catheter, and a microcomputer system controlling the moving device, the mechanical arm and the cantilever, wherein a control window is arranged on the rack, and controls the microcomputer system; and the reagent bottle comprises a waste liquid barrel, a flushing fluid reagent bottle part and a buffer fluid reagent bottle part. A liquid-based cell sheet producing method adopts the liquid-based cell sheet producing system. With the adoption of the technical scheme, the defects that a system operation mode is single, a procedure is solidified, a pipeline cannot be flushed automatically, a requirement on the quantity of specimens is strict, flux is low, energy consumption and reagent consumption material loss are great, a failure rate is high, maintenance is difficult, and equipment is costly are overcome.

The invention relates to 'the system displacement multiple gene magnification technology', which is characterized in that series target genes to be measured are displaced by a preselected probe into the same report sequence with different series probes, and a monotube magnification reaction of the same report sequence indicates multiple target molecules. The preselected probe is a target gene specific (/conservative) short sequence and is used as a detection magnification template, and a pair of adjacent probe area primers is arranged on the short template; the report sequence is a long-sequence nucleic acid single chain which is heterogenous with a target and is divided into left and right parts, and the 3' ends of the long-sequence nucleic acid single chain are preconnected with the probe area primers, and the report sequence combines a target gene template and extends. The displacement is started from the second round of cycle, the probe primers takes the extended report sequence as a template to synthesize a full-length report sequence including left and right probe sequences; and then a report system with a target probe is magnified by using the primers at both ends of the report sequence. The report sequences which are magnified by the report system and have different sizes or different codes are monitored to indicate the multiple target molecules indirectly. More than one set of report sequence can be alternated.

The invention discloses a method for rapid detection of BCR/ABL gene fusion by using fluorescence in-situ hybridization technology. The method is characterized in that detection of each target site needs three kinds of probes, i.e., contact probes, amplification probes and fluorescence probes; the 5' terminals of the contact probes specifically bind to a BCR gene (or an ABL gene), and the 3' terminals of the contact probes both have 20 sets of a repetitive sequence consisting of 20 bases and can specifically bind to the amplification probes; the 5' terminals of the amplification probes specifically bind to the 3' terminals of the contact probes, and the 3' terminals of the amplification probes both have 20 sets of a repetitive sequence consisting of 20 bases and can specifically bind to the fluorescence probes; the 5' terminals of the fluorescence probes are labeled by CY3 or FITC; and through synergism of the three kinds of probes, a fluorescence signal is amplified by 400 times, and thus, rapid and sensitive detection of BCR/ABL gene fusion is realized. The invention also discloses a kit and probe set for rapid detection of BCR/ABL gene fusion.

The invention discloses a method for preparing a single-cell suspension of aortic cells of a mouse. The method is characterized by specifically comprising the following steps of firstly, preparing 1 ml of an enzyme mixed solution, the surviving healthy wild mouse, an operating table of which the whole tabletop is a flat ice surface and a plurality of instruments; and then, performing intraperitoneal injection anesthesia on the prepared wild mouse by 200-500 microliters of chloral hydrate until the mouse is dead, immediately performing perfusion flushing on blood vessels of the wild mouse by 20ml of a phosphate buffer salt solution, performing overall disinfection on the dead wild mouse by alcohol with the volume fraction of 75%, performing thoracotomy andlaparotomy after disinfection is completed, and exposing the heart and the aorta. The whole operation process is completed on the operating table. Preparation of a single-cell suspension of vascular tissues of the mouse by using a shearing and mixed enzyme combined digestion process is simple, convenient and feasible, more immune cells exist, and an effective tissue single-cell suspension preparation method is provided for research of vascular immune cells.

The invention relates to a method for automatically preparing liquid-based cell slides, which is applied to pathological slide preparation equipment used in pathological examination. The method comprises the following steps of: cell isolation, filtration and settlement, cell suspension extraction and centrifugation and slide preparation. The method can automatically prepare slides on the same equipment and is high in automation degree, time-saving, labor-saving, quick and efficient. The number of slides can be changed flexibly. The method can be used for preparing a plurality of glass slides in one step, has wide application range, can automatically and accurately determine the cell extraction amount, ensures that the cell layer is thin and good in consistency, enables the background to be clean, and guarantees the uniformity and consistency in cell transfer amount on glass slides.

The invention relates to a moving-iron earphone, an inductor and a damping short-circuit ring of a transformer. An improved technology can be used for the damping short-circuit ring of the moving-ironearphone or the inductor and the transformer, particularly belongs to the damping short-circuit ring of the moving-iron earphone, and can improve tone quality and transparency. The method can also beused for inductance and the transformer of pulse signals to improve front and back edge characteristics and improve signal-to-noise ratio. The damping short-circuit ring is sleeved near a coil for generating the magnetic field, such as the outer side, so that the performance can be improved.

The present invention involves compositions and methods for assessing protein interactions in eukaryotic cells. Certain embodiments involve Retrovirus-Based Molecular Two-Hybrid Screens (ReMTH). ReMTH screens differ from other methods by performing screens in the native cellular hosts without cDNA library construction. Embodiments of the invention use the advantages of tagging endogenous genes with an exon encoding a marker fragment in combination with protein-fragment complementation assays (PCAs), which includes the complementation of at least two marker fragments to form a detectable marker complex. ReMTH vectors insert a nucleotide sequence encoding a first fragment of a marker, such as green fluorescent protein (GFP), into an endogenous gene resulting in expression of random endogenous genes tagged with a first marker fragment forming an endogenous prey protein or prey protein. ReMTH contain cells also express a bait protein that is fused with a second marker fragment. Prey/Bait interaction produces a reconstituted, detectable marker.

The invention provides a precise immunofluorescence labeling method for a whole tissue sample, which comprises the following steps: fixing, pretreatment, transparentizing treatment, confining liquid confining, freeze thawing and primary antibody incubation, and secondary antibody incubation. Freeze thawing and ultrasonic incubation are adopted, so that the permeability of an optical transparentizing agent and the compatibility of fluorescent protein are increased, the resolution of a fluorescence image is increased, the permeability of a large tissue sample is realized, and the overall immunolabeling of an organ is achieved.

The invention relates to a chromosome flaking method of a plant and concretely relates to a method for preparing a paeonia plant root tip chromosome slice. Compared with the prior art, the method is characterized in that the material taking range of a root tip is expanded to plants in a herbaceous peony group and in a Chinese peony group, in the dissociation step, a fixed material is put into a dissociation liquid with the volume ratio of absolute ethyl alcohol to 37% hydrochloric acid being 3: 1 to be dissociated at the room temperature; and a low-permeability step in distilled water is alsoadded. Herbaceous peony and Chinese peony seedlings in a field are taken as raw materials, a special dissociation step is adopted, on one hand, the conditions required by the experiment is reduced, the experiment is more convenient; on the other hand, the concentration of hydrochloric acid is properly increased such that the influence of cell walls and cytoplasm on the flaking result is reduced, and meanwhile, the breakage and decomposition of chromosomes under a high-temperature acidic condition are also prevented; after dissociation, hyposmosis enables the chromosomes to be well dispersed, the counting is convenient, and the background is clean.

The invention relates to the detection of protein glycosyl, in particular to an application of a compound as a blocking reagent for glycosyl on an antibody. To solve the problem of the interference ofthe glycosyl of a current antibody on detection, a compound used as a blocking reagent for the glycosyl on the antibody is provided, and the compound is selected from one of alpha-semicarbazide, acetylhydrazine, sulfur acetohydrazide, thiosemicarbazide, asparagine hydrazide, tyrosine hydrazine, N (benzyloxycarbonyl)-L-valyl-L-tyrosine hydrazide, or N (benzyloxycarbonyl) glycinic hydrazide. The compound used as the blocking reagent for the glycosyl on the antibody has the advantages of being capable of conducting petreatment of antibodies, avoiding binding of plant lectins to antibodies, guaranteeing accurate quantification of the glycosyl on captured proteins, being simple in the process of blocking the antibodies with one step reaction needed, shortening the reaction process of existingblocked antibodies, and significantly reducing costs.

The invention relates to a photographic device used for photography of inset specimens and a photographic method thereof and belongs to the technical field of special operation devices for photography. The photographic device includes a connecting rod, a camera and a supporting rod; a connecting buckle is arranged on the connecting rod, one end of the connecting buckle sleeves the connecting rod and moves up and down along the connecting rod, and the other end of the connecting buckle is used for installing the camera to achieve fixation of the position of the camera; the supporting rod is positioned below the camera, a sample pile is arranged on the supporting rod and used for placing a sample to be photographed, and the spacing between the sample to be photographed and a lens of the camera is 15-50 cm. The photographic device is applied to photography of specimens or samples of animals and plants such as insects and butterflies and has the advantages of being simple, effective, clearin photography, clean in background and the like.

The invention discloses a high-efficiency extraction method of macadamia nut leaf total proteins. The method comprises the following steps: grinding and crushing fresh macadamia nut leaves by using liquid nitrogen; adding a pre-cooling cracking liquid PW1 for primary extraction; performing solid-liquid separation to obtain a first precipitate; cleaning and airing the first precipitate; adding an extracting solution PW2 for secondary extraction; performing solid-liquid separation to obtain a second supernatant; adding a pre-cooling methanol solution; performing centrifuging to obtain a second precipitate; cleaning and drying the second precipitate; adding a dissolving solution PW3 for tertiary extraction; and performing solid-liquid separation to obtain a third supernatant, namely the macadamia nut leaf total proteins. According to the invention, the fresh macadamia nut leaves are taken as materials, and the three extracting solutions are added into macadamia nut leaf powder, so that oxidation of raw materials can be effectively avoided, degradation of proteins can be avoided, the yield of the total proteins is high, purity of the extracted protein is high, the SDS-PAGE electrophoresis bands are neat and clear, and the macadamia nut leaf powder is suitable for Western Blot analysis.

The invention provides a thin-layer cytology film-making device, which includes a carrying box, a glass slide, a fixed chamber and a filter chamber. Wherein, there are two corresponding card slots on one edge of the carrying box, and a circular hole on the other end. The slides are chemically treated to have an electrical charge on the surface. The upper part of the fixed warehouse has anti-skid bumps, the bottom is embedded with sealing gaskets, and the bottom edge has two protruding fixed wings. There is a layer of filter membrane at the bottom of the filter chamber. The filter membrane is a filter membrane with a small pore size. The slide glass is in the carrying box, the fixed compartment rotates clockwise and snaps into the slot of the carrying box to fix the slide glass, and the filter compartment is located on the upper part of the fixed compartment. The thin-layer cytology film production device can be applied to various cytology detection items, saving the application cost. In terms of application, the produced cytology film is clean and beautiful, which greatly facilitates the doctor to make a diagnosis.

The invention provides a myeloid tissue decalcification solution and a method for preparing myeloid tissue paraffin sections. The myeloid tissue decalcification solution is composed of hydrochloric acid, formaldehyde and distilled water, wherein the volume percentage concentration of the hydrochloric acid in the myeloid tissue decalcification solution is 1-3%, and the volume percentage concentration of the formaldehyde in the myeloid tissue decalcification solution is 9-11%. According to the myeloid tissue decalcification solution and the method for preparing the myeloid tissue paraffin sections, the myeloid tissue decalcification solution is used for decalcifying a myeloid tissue specimen, the specimen pretreatment process is shortened, the report time is short, timely diagnosis is facilitated, and the obtained HE section shows that the cell morphology is well preserved and the tissue structure is clear; the immunohistochemical staining sections show that the staining effect is excellent, the positioning is accurate, and the background is clean; and and the phenomenon of excessive decalcification of the sample is avoided.

The invention discloses a separation device, which comprises a sample collector, a filter, a connector, a capturer and a filtrate collector, the sample collector and the filtrate collector are both containers, the filter comprises a filter screen, the capturer comprises a capture chip with the aperture of 0.01-1000 [mu] m, the connector is a barrel-shaped object, and the sample collector, the filter, the connector, the capturer and the filtrate collector are connected in sequence. According to the separating device disclosed by the invention, before samples such as clinical hydrothorax and ascites, urine and the like are transferred to a laboratory for detection, particulate matters to be detected in the samples can be separated and fixed at the same time at a sampling site, the intact forms of cells are kept, the cells are prevented from disintegration and degradation of biomacromolecules in the cells are prevented, and microbial pollution and reproduction are avoided; the false negative rate caused by the storage and transportation process of the sample is effectively reduced, and the detection rate of tumor cells is greatly improved.

The invention relates to the technical field of biology, in particular to a DNA ploidy staining solution as well as a preparation method and a staining method thereof, and the prepared DNA staining solution can solve the problems that the background is easy to co-stain, the staining time is relatively long and slices are easy to fade in flaking, so that a reliable histochemical staining technologyis provided for tumor cell nucleus DNA content image determination. Alcohol with the concentration of 95% is used for fixing cell nucleuses, the situation that in the dyeing hydrolysis process of unfixed cell nucleuses, DNA can form small fragments and dissociate out of the cell nucleuses, the DNA measurement accuracy is affected is avoided, aldehyde groups generated before hydrolysis can be sealed through fixation, and false positive products are reduced. Meanwhile, the BS stationary liquid can be used for changing the space structure of DNA molecules, so that the sensitivity to acid hydrolysis is influenced, and the situation that chromatin coloring is relatively light due to improper selection of the stationary liquid or too long tissue fixing time and relatively large difference in strength of dyeing reaction is avoided. Through gradient alcohol dehydration, the DNA staining result is clear, and the background is cleaner.