46results about How to "Clean background" patented technology

The invention discloses a manufacturing method and application of an insect in-vitro protein interaction detecting system. At the positions of amino acid residues at the 159th bit and the 160th bit, mCherry is divided into a segment NmC and a segment CmC through a PCR method, and then the segment NmC and the segment CmC are each fused with a connecting zone sequence, a c-Myc label and a poly(A) sequence to form four fused segments; the four fused segments are each connected with a pIE-MCS plasmid with an autographa californica multiple nuclear polyhedrosis virus ie1 gene promoter and a gp64 gene poly(A) sequence to establish expression plasmids; the expression plasmids are combined to form an insect cell dimolecular fluorescent complementary detecting system. The method is simple and easy to implement, and the established dimolecular fluorescent complementary detecting system expands the application of an insect cell expression system and provides a convenient and effective technological means for insect proteomics and research on protein interaction between insects and pathogenic microorganisms of the insects.

The invention discloses a capture instrument for circulating tumor cells. The capture instrument comprises a multi-way valve device, a wheel disc device, a sample loading needle device and an injector device, wherein the multi-way valve device is connected with a reagent bag, different kinds of reagents can be selected through the multi-way valve device, the wheel disc device is provided with a filter, the sample loading needle device can be inserted into the filter of the wheel disc device so as to be communicated with an air path and a liquid path, the injector device absorbs the required reagent through the multi-way valve device and then injects the reagent into the filter. Furthermore, the invention also discloses a method for capturing and staining by using the capture instrument for circulating tumor cells. The retention rate of the capture instrument in capturing tumor cells is higher than that of manual capturing; furthermore, compared with manual staining, the capture instrument for circulating tumor cells has high degree of uniformity and consistency in staining, and is clean in background.

The invention discloses a high-efficiency extraction method for the total protein of macadamia nut leaves. The method includes the following steps: after crushing the fresh leaves of macadamia nuts with liquid nitrogen, adding pre-cooled lysate PW1 for primary extraction, obtaining the first precipitate after solid-liquid separation, cleaning and drying the first precipitate; adding extraction solution PW2 for secondary extraction Extraction, after solid-liquid separation to obtain the second supernatant, add pre-cooled methanol solution, centrifuge to obtain the second precipitate, wash and dry the second precipitate; add the dissolving solution PW3 for three extractions, and obtain the second precipitate after solid-liquid separation Three supernatants, that is. The present invention uses fresh macadamia nut leaves as materials, and adding three kinds of extracts of the present invention to the macadamia nut leaf powder can effectively avoid raw material oxidation and protein degradation, the total protein yield is high, and the extracted protein has high purity, and SDS-PAGE electrophoresis strips The bands are neat and clear, and suitable for Western Blot analysis.

The invention discloses an immunochromatography assay and a device taking a color-developing agent as a sample carrier and capable of repeated sample adding, and the method comprises: 1) preparing a reagent strip according to the conventional method; 2) preparing the color-developing agent according to the conventional method, and mixing the corresponding antigen / antibody of a sample under test with the color-developing agent to form a color-developing agent mixture; 3) adding 50mu l-5ml color-developing agent mixture to a color-developing agent sample adding region; and 4) adding 5mu l-100mu l sample under test to the sample adding region when the color-developing agent enters a sample adding region of a nitrocellulose membrane, and reading the result within 1-30min, wherein, sample adding can be repeated in the meantime. Compared with other conventional methods, in the invention, the color-developing agent enters the nitrocellulose membrane earlier than the sample and has continuous follow-up supply, so the dynamic effects enable the invention to have the advantages of less amount of sample each time, shortened interpretation time, high sensitivity in unit time, clean background of the nitrocellulose membrane after reaction and the like.