Static electricity spinning prepared fixed enzymic electrode and method thereof

An electrospinning and enzyme electrode technology is applied in the field of immobilized enzyme electrodes and immobilized enzyme electrodes to achieve the effects of simple preparation method, good response performance and improved electron transport capability

Inactive Publication Date: 2006-02-22
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Bin Ding et al. proposed to use PVA and PAA co-spinning method to construct NH3 affinity sensors (Bin Ding, JinhoKim, Yasuo Miyazaki, [J]. Sensors and Actuators B, 2004, 101: 373-380), but by electrospinning The method of immobilizing enzymes to construct amperometric biosensors and biofuel cells has not been reported in the literature.

Method used

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  • Static electricity spinning prepared fixed enzymic electrode and method thereof
  • Static electricity spinning prepared fixed enzymic electrode and method thereof
  • Static electricity spinning prepared fixed enzymic electrode and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Use Al with uniform particle size for the gold substrate 2 O 3The powder is polished on deer skin, washed repeatedly with twice distilled water, and then placed in a phosphate buffer solution (PBS) with pH=6.8 and scanned by cyclic voltammetry until it is stable. Weigh the PVA into deionized water, heat and stir in a water bath to completely dissolve the PVA, and cool to room temperature under stirring to prepare a PVA aqueous solution with a concentration mass volume ratio of 8%. Take 5ml of the prepared PVA aqueous solution and add 198μL of glucose oxidase to ensure that the GOD concentration in the solution is 200U / ml. 120μL of nano-gold solution was added to the PVA aqueous solution. The PVA / glucose oxidase mixed solution added with the nano gold solution is injected into a syringe with a needle, and the syringe is fixed on the syringe pump. Place a grounded receiving screen (aluminum foil) at a distance of 10 cm from the needle, and open a hole in the center of the alu...

Embodiment 2

[0031] The copper electrode was used as the base electrode of the working electrode, and the others were the same as in Example 1.

[0032] image 3 This is a comparison between the electrode without enzyme and the electrode with enzyme on the copper electrode substrate. (A) Upper 3364cm -1 The broad peaks on the left and right are the stretching vibration peaks of O-H in PVA, and are also the characteristic absorption peaks of PVA; 2943cm -1 Left and right are -CH 2 Peak of stretching vibration; 1420cm -1 Left and right are O-H and C-H bending vibration and -CH 2 Absorption peak caused by bending vibration; 1096cm -1 Left and right are the absorption peaks of C-O (multi-associative) single bond stretching and O-H bending vibration, 854cm -1 The left and right are characteristic peaks related to the isotactic sequence. The characteristic absorption peak of PVA appears on the PVA / GOD spectrum [Figure (B)]. At the same time, since the stretching vibration peak of N-H in the glucose...

Embodiment 3

[0034] The platinum electrode is used as the base electrode of the working electrode, and the other is the same as in Example 1.

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Abstract

This invention discloses one static fabric processed fixed enzyme electrode and its method, which belongs to fixed enzyme electrode technique, wherein, the said enzyme electrode is composed of electrode base, reacting layer composed of polymer nanometer fiber film and oxidation and deoxidizing enzyme or the reacting layer is composed of polymer nanometer fiber film and oxidation and deoxidizing enzyme and metal particles with 5 to 100 nanometer electric activity or the reacting layer is composed of polymer nanometer fiber film and film oxidation and deoxidizing enzyme and electro medium.

Description

Technical field [0001] The invention relates to an immobilized enzyme electrode prepared by electrospinning and a method thereof, belonging to immobilized enzyme electrode technology. Background technique [0002] The immobilized enzyme electrode is to fix the biocatalytic enzyme on the surface of the electrode material, and obtain the detailed information of the component from the electrode potential and response current generated by the enzyme-catalyzed electrode reaction. Immobilized enzyme electrodes are key components of biosensors and biofuel cells. Among them, as a new type of sensing technology that has emerged in recent decades, biosensors have applications in clinical diagnosis, food and drug analysis, environmental analysis, biopharmaceutical development, industrial control, military, biochips, and biotechnology. Biofuel cells can use organic matter in living organisms as fuel to generate electricity, which is a clean, green, and purely natural renewable energy source....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
Inventor 许鑫华陈强刘强袁晓燕任光雷
Owner TIANJIN UNIV
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