3results about How to "Increase response current" patented technology

The application discloses an enzyme-electron medium micro-nano composite for biosensing and a preparation method and application thereof. A dehydrogenase for electrochemical detection and an electron medium are jointly loaded in a cavity of a carrier, the carrier is contracted through chemical or physical stimulation, and electron medium molecules in the cavity are forced to be distributed around active centers of the dehydrogenase, so that a contractible limited microsphere structure is obtained. The enzyme-electron medium micro-nano composite obtained by the application can significantly improve a response current generated by unit enzyme catalysis, enhance the stability of the enzyme, and effectively prolong the service life of a sensor.

The application discloses a chemical modified electrode for detecting furazolidone and an electrochemical detection method thereof, and relates to the technical field of electrochemical detection, and comprises the following steps: (1) preparation of longan carbon; (2) preparation of nitrogen-doped longan carbon material (NCs); (3) preparation of nitrogen-doped longan carbon-polydopamine material (NCs-PDA); (4) preparation of NCs-PDA / SPCE modified electrode; (5) electrochemical detection of FZD; and (6) characterization of NCs-PDA / SPCE. The chemical modified electrode for detecting furazolidone and the electrochemical detection method thereof use longan shells as a carbon source, chitosan and urea as nitrogen sources, and prepare a nitrogen-doped porous carbon material through a high-temperature carbonization method, combine polydopamine and a portable disposable screen-printed carbon electrode (SPCE), prepare an electrochemical modified electrode NCs-PDA / SPCE for detecting furazolidone, and establish an electrochemical rapid detection method for detecting furazolidone in animal-derived food based on the modified electrode.

The application discloses an electrochemical detection method of 3-amino-2-oxazolidinone (AOZ), and relates to the technical field of electrochemical detection, and comprises the following steps: (1) preparation of conjugated microporous polymer (CMP); (2) preparation of gold nanoparticles (AuNPs); (3) preparation of CMP / AuNPs / SPCE; (4) electrochemical detection of AOZ; and (5) characterization of CMP / AuNPs / SPCE. The electrochemical detection method of 3-amino-2-oxazolidinone is characterized in that gold nanoparticles with a large specific surface area and excellent conductivity and conjugated microporous polymer are drop-coated on the surface of a screen-printed carbon electrode (SPCE) to prepare an electrochemical modified electrode CMP / AuNPs / SPCE for measuring AOZ, and a differential pulse voltammetry method is used to establish an electrochemical detection method for measuring AOZ based on the modified electrode. The method is convenient for batch preparation and low in cost, only needs 30 muL of sample, and can be used for on-site rapid detection of AOZ, a furazolidone metabolite, in animal-derived food.