Fast and efficient preparation method of pig A type clostridium perfringens inactivated vaccine

A technology of Clostridium perfringens and inactivated vaccines, applied in vaccines, veterinary vaccines, medical preparations containing active ingredients, etc., can solve the problem of inability to obtain safe and good immune effect pig vaccines, severe vaccination reactions of livestock, The preparation process is cumbersome and other problems, and the effect of shortening the production time, less allergic reaction and simple process is achieved.

Active Publication Date: 2018-07-13
鄂尔多斯市旭和畜牧有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the researches on inactivated vaccines of Clostridium perfringens type A are aimed at deer, rabbits, and cattle. There are few researches on the inactivated vaccines of Clostridium perfringens type A in pigs, and simple replacement cannot obtain safe It is a porcine vaccine with good sex, high stability and good immune effect, and the inactivation reagent of Clostridium perfringens in the prior art uses formaldehyde with carcinogenic effect, and the adjuvant mostly adopts aluminum hydroxide gel containing heavy metals
There are still some problems that need to be further solved in the use of these vaccines: (1) the livestock vaccination reaction is relatively heavy; (2) the injection dose is large, and it is inconvenient to use; (3) the preparation process is cumbersome and takes a long time

Method used

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  • Fast and efficient preparation method of pig A type clostridium perfringens inactivated vaccine
  • Fast and efficient preparation method of pig A type clostridium perfringens inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] A fast and efficient preparation method of porcine type A Clostridium perfringens inactivated vaccine:

[0050] (1) Acquisition of pathogenic bacteria: pick the disease material of the dead pigs caused by Clostridium perfringens type A, scrape the intestinal contents, and refrigerate at 2°C;

[0051] (2) Strain cultivation: Autoclave the synthetic medium of pure milk and glucose in a pressure cooker at a temperature of 110°C and a sterilization time of 20 minutes; when the temperature drops to 80°C, pour the disease material into the pressure cooker Inside, 44°C high-temperature anaerobic culture for 4 hours for passage, passage 3 times;

[0052] (3) Bacterial liquid preparation: take the three-passage bacterial liquid in step (2) and insert it into the meat liver gastric membrane digestion soup for 8 hours at 37°C. The preparation of gastric membrane digestion soup includes the following steps: crush 100g of beef, 100g of beef liver, and 150g of pig gastric mucosa, ad...

Embodiment 2

[0056] A fast and efficient preparation method of porcine type A Clostridium perfringens inactivated vaccine:

[0057] (1) Acquisition of pathogenic bacteria: pick the disease material of the dead pigs caused by Clostridium perfringens type A, scrape the intestinal contents, and refrigerate at 5°C;

[0058] (2) Strain cultivation: Autoclave the synthetic medium of pure milk and glucose in a pressure cooker at a temperature of 110°C and a sterilization time of 20 minutes; when the temperature drops to 80°C, pour the disease material into the pressure cooker Inside, 45°C high-temperature anaerobic culture for 4 hours for passage, passage 3 times;

[0059] (3) Bacterial liquid preparation: take the three-passage bacterial liquid in step (2) and insert it into the meat liver gastric membrane digestion soup for 10 hours at 37°C. The preparation of gastric membrane digestion soup includes the following steps: crush 100g of beef, 100g of beef liver, and 150g of pig gastric mucosa, a...

Embodiment 3

[0063] A fast and efficient preparation method of porcine type A Clostridium perfringens inactivated vaccine:

[0064] (1) Acquisition of pathogenic bacteria: pick the disease material of the dead pigs caused by Clostridium perfringens type A, scrape the intestinal contents, and refrigerate at 4°C;

[0065] (2) Strain cultivation: Autoclave the synthetic medium of pure milk and glucose in a pressure cooker at a temperature of 110°C and a sterilization time of 20 minutes; when the temperature drops to 80°C, pour the disease material into the pressure cooker Inside, 42°C high-temperature anaerobic culture for 6 hours for passage, passage 3 times;

[0066] (3) Bacterial liquid preparation: take the three-passage bacterial liquid in step (2) and insert it into the meat liver gastric membrane digestion soup for 6 hours at 37°C. The preparation of gastric membrane digestion soup includes the following steps: crush 100g of beef, 100g of beef liver, and 150g of pig gastric mucosa, ad...

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Abstract

The invention belongs to the technical field of veterinary biological products, and relates to a fast and efficient preparation method of a pig A type clostridium perfringens inactivated vaccine. Themethod comprises the following steps of picking a suspected sample of an A type clostridium perfringens lethal diseased pig, scraping an intestinal content, and refrigerating or freezing; autoclavinga culture medium in an autoclave, after temperature reducing, pouring the suspected sample in the autoclave, carrying out anaerobic culture, and carrying out passage for three times; inoculating bacteria liquid subjected to three-time passage into a meat liver-stomach membrane digestion soup, culturing, and centrifuging after finishing culturing, thus obtaining a supernatant liquor; adding an inactivator into the supernatant liquor, fully shaking out, inactivating, and detoxifying to obtain inactivated bacteria liquid; mixing the inactivated bacteria liquid and an adjuvant to obtain the inactivated vaccine. The method provided by the invention has the advantages of simple process, low cost, and low pollution; the prepared inactivated vaccine has the advantages of high safety, no adverse reaction after being injected, high immunization efficiency and long immunity period.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a rapid and efficient preparation method of an inactivated porcine type A Clostridium perfringens vaccine. Background technique [0002] Clostridium perfringens, also known as Clostridium welchii, can produce a variety of exotoxins and enzymes, which can decompose sugar in muscle and connective tissue, produce a large amount of gas, cause severe emphysema to the tissue, and then affect the blood supply, causing tissue Extensive necrosis. According to the main toxins synthesized and secreted by Clostridium perfringens, they are divided into five types of toxins: A, B, C, D, and E; among them, Clostridium perfringens type A mainly causes gastrointestinal lesions, which is human and animal gas The main pathogenic bacteria of sexual gangrene, rabbit clostridial diarrhea, horse necrotic enteritis and human food poisoning are also the main pathogens ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/08A61K39/39A61P31/04
CPCA61K39/08A61K39/39A61K2039/521A61K2039/552A61K2039/55577
Inventor 杨耀光张俊清郝俊飞屈建平杨耀军黄建华邵春华
Owner 鄂尔多斯市旭和畜牧有限责任公司
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