198results about How to "Increase virulence" patented technology

The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and / or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.

The present invention concerns methods and compositions for the treatment of cancer and cancer cells using altered poxviruses, including a vaccinia virus that has been altered to generate a more effective therapeutic agent. Such poxviruses are engineered to be attenuated or weakened in their ability to affect normal cells. In some embodiments, methods and compositions involve poxviruses that possess mutations that result in poxviruses with diminished or eliminated capability to implement an antiviral response in a host. Poxviruses with these mutations in combination with other mutations can be employed for more effective treatment of cancer.

The invention relates to avermectin B2a / 2b amine derivatives and derivative salts thereof, and a preparation method and an application of the avermectin B2a / 2b amine derivative salts. The avermectin B2a / 2b amine derivatives comprise six types of compounds. The avermectin B2a / 2b amine derivative salts are salts formed by reactions of the six types of avermectin B2a / 2b amine derivatives and organic acid and inorganic acid. The preparation method of the avermectin B2a / 2b amine derivative salts comprises the steps that: (1) avermectin B2a / 2b is subjected to a protective reaction with tert-butyl dimethyl chlorosilane or allyl chloroformate; (2) protected B2a / 2b is subjected to a reaction with an oxidant, such that an avermectin B2a / 2b ketone compound is obtained; (3) the ketone compound is subjected to an amination reaction, such that protected amine derivative is obtained; and (4) the protective agent is removed, such that B2a / 2b amine derivative is obtained; the derivative is subjected to a reaction with organic or inorganic acid, such that avermectin B2a / 2b amine derivative salt is obtained. According to the invention, avermectin B2 is adopted as a matrix for synthesizing avermectin B2a / 2b amine derivative and avermectin B2a / 2b amine derivative salt, such that avermectin B2 applciation range is widened.

A method of predicting the virulence of a new or uncharacterized PRRS virus isolate is provided wherein the isolate is injected into swine and allowed to replicate for a period of from about 3-15 days. During this period, the rate of virus growth and / or the magnitude of viremia is determined, and this data is compared with a corresponding growth rate and / or viremia magnitude of a PRRS virus isolate of known virulence, as a measure of the virulence of the new or uncharacterized isolate. Additionally, a method of selecting an isolate for inclusion in an immunogenic composition based on the predicted virulence is also provided, together with compositions incorporating attenuated forms of viruses predicted to be virulent.

The present invention concerns methods and compositions for the treatment of cancer and cancer cells using altered poxviruses, including a vaccinia virus that has been altered to generate a more effective therapeutic agent. Such poxviruses are engineered to be attenuated or weakened in their ability to affect normal cells. In some embodiments, methods and compositions involve poxviruses that possess mutations that result in poxviruses with diminished or eliminated capability to implement an antiviral response in a host. Poxviruses with these mutations in combination with other mutations can be employed for more effective treatment of cancer.

A method of predicting the virulence of a new or uncharacterized PRRS virus isolate is provided wherein the isolate is injected into swine and allowed to replicate for a period of from about 3-15 days. During this period, the rate of virus growth and / or the magnitude of viremia is determined, and this data is compared with a corresponding growth rate and / or viremia magnitude of a PRRS virus isolate of known virulence, as a measure of the virulence of the new or uncharacterized isolate. Additionally, a method of selecting an isolate for inclusion in an immunogenic composition based on the predicted virulence is also provided, together with compositions incorporating attenuated forms of viruses predicted to be virulent.

The invention provides a Bacillus thuringiensis new bacterial strain HS18-1 with the preserving number CGMCC No.2718. Shown by the toxicity test of HS18-1, the HS18-1 has enormous toxicity towards Lepidoptera and Diptera insets. The Bacillus thuringiensis HS18-1 can be prepared into insecticides for the prevention and control of major crop insects, thus facilitating the diversification and systemization of Bacillus thuringiensis insecticide products, enlarging the usage scope of Bacillus thuringiensis insecticides, reducing the use of agricultural chemical, alleviating environment pollution, and having significant economic value and application prospect.

The invention discloses a composition, kit and method for detecting high-virulence bacterial strains and / or toxin type of clostridium difficile. The composition and kit comprise primers, probes and standard substances, wherein the nucleotide sequence of the primers is as shown in SEQ ID NO. 1-47; the high-virulence bacterial strains and / or toxin type of clostridium difficile can be detected through fluorogenic quantitative PCR by adopting the composition or kit. According to the invention, the sensitivity and specificity are high; the toxin type and virulence of clostridium difficile can be accurately judged through detecting tcdC gene mutation or deletion and coordinating with virulence gene detection, including tcd A / B and binary virulence gene cdt A / B, of clostridium difficile.

Certain multi-cyclic compounds and compositions thereof are useful for reducing or inhibiting the growth of bacterial biofilms and for controlling bacterial biofilm infections. Such compounds and compositions are also useful in methods for reducing or inhibiting the growth of biofilms and for controlling bacterial biofilm infections involving biofilms.

The invention provides a novel strain BtMC28 of Bacillus thuringiensis, which has a preservation number of CGMCC No.2719. Tests of the virulence activity of the BtMC28 show that the BtMC28 has extremely high virulence on Lepidoptera, dipteral pests, and the like. The Bacillus thuringiensis BtMC28 can be made into a pesticide for controlling major farm crop pests. As a result, the novel strain BtMC28 of Bacillus thuringiensis diversifies and serializes the products of Bacillus thuringiensis pesticides, enlarges the application range of the Bacillus thuringiensis pesticides, reduces the pesticide dosage and environmental pollution, and has significant economic value and application prospect.

The invention discloses an anti-Nilaparvata-lugens pesticidal protein, and a coding gene and application thereof. The protein is a) or b) as follows: a) protein composed of amino acid sequence disclosed as Sequence 2 in the sequence table; or b) protein with same functions subjected to substitution and / or deletion and / or addition of one or more amino acid residues on amino acid sequence disclosed as Sequence 2 in the sequence table. The experiment proves that the novel pesticidal protein disclosed by the invention has high toxicity for Nilaparvata lugens, provides a new strategy for resisting Nilaparvata lugens and has wide application prospects.

The invention relates to the field of diagnosis of and vaccination against Streptococcal infections and to the detection of virulence markers of Streptococci. The invention discloses a method for modulating virulence of a Streptococcus, the method comprising modifying a genomic fragment of Streptococcus wherein the genomic fragment comprises at least a functional part of a fragment identifiable by hybridization in Streptococcus suis to a nucleic acid or fragment thereof as shown in FIG. 5.

The invention relates to an insecticidal gene cry2Ah-like with high toxicity on lepidoptera pests and an application thereof, and belongs to the technical field of biology. The protein sequences of insecticidal proteins Cry2Ah-like, Cry2Ah-likesp and Cry2Ah-likevp with high toxicity on the lepidoptera pests are respectively shown as SEQ ID NO. 1, SEQ ID NO. 2 and SEQ ID NO. 3. The new gene provided by the invention achieves the high toxicity on cotton bollworm and corn borer, enriches the insecticidal gene resources, shows the toxicity on relative pests by being applied to transform microbes and overcomes and postpones the drug resistance of the pests on engineering bacteria.

The prevention and treatment of influenza is described, by using passive immunization with antibodies (e.g. polyclonal, monoclonal, etc.) to one or more conserved influenza proteins. Both antibodies and fragments thereof are contemplated, raised against conserved proteins such as nucleoproteins.

The present invention provides methods for identifying viral virulence factors and for identifying cellular polypeptides to which the viral polypeptides bind. The cellular polypeptide is useful as a therapeutic target or as a therapeutic agent for treating diseases and disorders, including immunological diseases or disorders.

The invention relates to a method for producing rotavirus vaccines in large scale by utilizing a bioreactor, and in particular relates to a method for culturing rotavirus inactivated vaccines in large scale by utilizing a 75L bioreactor. The method comprises the following steps: (1) carrying out reviving and passage on Vero cells; (2) collecting the cells and inoculating the cells into the bioreactor; (3) culturing the cells in the bioreactor; (4) changing the liquid and washing the cells; (5) activating and infecting rotaviruses; and (6) carrying out maintenance culture and harvesting on the rotaviruses. The method aims at overcoming the defect that in the large-scale production, the rotaviruses can not release cells easily, so that the obtained viruses are low in toxicity titer, and the inactivated rotavirus vaccines with high titer and high yield are obtained; the method also aims at overcoming the defect that in the large-scale production, the adsorption force and infection force of the rotaviruses are poor, and thus the yield and quality of the rotaviruses are greatly improved.

The invention provides a method for pretreating beer waste for biochemical utilization. The method comprises the following steps: carrying out ozone wall breaking and sterilization on one or mixture of beer waste water, sludge, waste and fresh beer yeast slurry so as to increase leaching of nutrient substances of yeast content, wherein the ozone admission amount is 0.3-0.8g of ozone / g of waste beer dried substances; and carrying out high-pressure steam sterilization on samples subjected to ozone treatment at 121 DEG C for 45 minutes, thus the samples subjected to high-pressure steam sterilization can used for microorganism yeast culture. The beer waste pretreated by the method provided by the invention has the advantages that the nutrient substance release rate can be greatly improved, the content of amino nitrogen in a culture medium is improved by 4-5 times, and the amino nitrogen yield achieves 3-5 percent, thus the pretreated beer waste serving as the culture medium is beneficialto microorganism culture.

The invention provides a new bacterial strain that can prevent and control plant pathogenic fungi and kill acarids—streptomyces S1-5 and its application in resisting plant pathogenic fungi and killing acarids. Streptomyces sp.S1-5 (Streptomyces sp.S1-5), preserved in China Center for Type Culture Collection, date of preservation: May 24, 2007, preservation number CCTCC No: M 207073. The pesticide prepared by the present invention can achieve the effect of killing fungicides and killing acarids by using it once, greatly reducing agricultural costs and lightening the burden on farmers; and the duration of effect is as long as 30 days, and it is also safe for humans and animals, does not kill natural enemies and is beneficial Insects are not easy to produce resistance to control objects, do not pollute the environment, can be rapidly degraded by soil microorganisms without affecting soil fertility and microbial flora, have no bioaccumulation, and are very safe for ecology. They are a key enzyme inhibitor. Pesticides, no cross-resistance with other insecticides.

Non-naturally occurring mycobacteria in the Mycobacterium tuberculosis complex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin from a mycobacterium in the M. tuberculosis complex.

A natural pyrethrin microemulsion as an insecticide for preventing and killing the most of agricultural and vegetable pests is prepared from natural pyrethrin, natural assistant, chemical assistant, and water.

The invention relates to C2H2 type zinc finger protein BbAzf of beauveria bassiana. The C2H2 type zinc finger protein BbAzf has the nucleotide sequence as shown in SEQIDNO.1 and the amino acid sequence as shown in SEQIDNO.2. Oosporein has antibacterial antiviral antineoplastic activity. The yield of the oosporein in the beauveria bassiana can be improved by means of damaging genes of the beauveria bassiana and therefore the obtained beauveria bassiana gene knockout carrier has high toxicity towards agriculture and forestry pests.

Non-naturally occurring mycobacteria in the Mycobacterium tuberculosis complex are provided. These mycobacteria have a deletion of an RD1 region or a region controlling production of a vitamin, and exhibit attenuated virulence in a mammal when compared to the mycobacteria without the deletion. Also provided are non-naturally occurring mycobacteria that have a deletion of a region controlling production of lysine, and mycobacteria comprising two attenuating deletions. Vaccines comprising these mycobacteria are also provided, as are methods of protecting mammals from virulent mycobacteria using the vaccines. Also provided are methods of preparing these vaccines which include the step of deleting an RD1 region or a region controlling production of a vitamin or the amino acids leucine and lysine from a mycobacterium in the M. tuberculosis complex. Embodiments of these mycobacteria, vaccines and methods, encompassing mycobacteria comprising a leucine auxotrophy and a pantothenate auxotrophy, are also provided.

The invention relates to the field of plant disease prevention and control, in particular to application of farnesol as an antibacterial synergist in preventing and controlling plant grey mold. Particularly, the antibacterial synergist is applied to preventing and controlling the grey mold with a benzimidazole bactericide or triazole bactericide. The farnesol has the synergistic action to the bactericide, can improve the virulence of the bactericide by being used mixing with the bactericide, improves the sensibility of a drug-resistant strain to the bactericide and the prevention and control effect of the bactericide to the field drug-resistant grey mold, reduces the dosage of the bactericide and provides a new method to development and application of a novel preparation of the existing bactericide. Meanwhile, the farnesol is an extract of natural plants and is environmentally friendly. The invention provides a novel application of the drug, can be used for disease prevention and control in the agricultural field and provides a new way to grey mold field drug-resistant government.

The invention discloses application of a gene FoPao in regulation and control of pathogenicity of banana fusarium wilt, and belongs to the field of plant genetic engineering. The application comprisesthe following steps: constructing a gene knockout vector, and introducing the vector into a banana fusarium wilt protoplast; and knocking out the gene from banana fusarium wilt by using a homologousrecombination method to obtain a knockout mutant delta FoPao. The gene supplementing vector is constructed, the gene supplementing vector is introduced into the delta FoPao protoplast, and the gene issupplemented back to the knockout mutant by using a random insertion method to obtain the supplemented mutant delta FoPao-com. Pathogenicity tests show that the deletion of the gene FoPao significantly increases the toxicity of banana fusarium wilt; and after the gene is supplemented, the toxicity of the supplemented mutant is consistent with the wild type of the banana fusarium wilt. The invention proves that the FoPao is related to pathogenicity of banana fusarium wilt. The research is helpful to deeply clarify the pathogenic molecular mechanism of banana fusarium wilt.

The invention discloses a recombinant serine protease inhibitor, a fungus expression vector and a fungus insecticide of recombinant protein. The invention relates to the recombinant serine protease inhibitor; a molecular biology technology is used to construct a recombinant nucleotide sequence; and the recombinant serine protease inhibitor comprises a signal peptide sequence of encoded coccidio beauveriabassiana chitinase and a serine protease inhibitor gene of encoded drosophila, and has a nucleotide sequence shown as SEQ ID NO. 1 and an amino acid sequence shown as SEQ ID NO. 2. The obtained recombinant serine protease inhibitor can inhibit immunoreaction of insects, increase multiplication speed of fungi in haemocoeles, and therefore improve characteristics of insecticide fungus toxicity.

The invention relates to insecticide gene and application thereof. A nucleotide sequence of the insecticide gene comprises a sequence (a) or a sequence (b), wherein the (a) has a nucleotide sequence shown in an SEQ ID NO: 1, and the (b) is a nucleotide sequence cross bred with the nucleotide sequence limited by the (a) on strict conditions. The insecticide gene is suitable for being expressed in corns, not only remarkably improves protein expression index and stability of CrylAb insecticide protein, and remarkably enhances toxicity of the CrylAb insecticide protein on main insects of corns.

The invention relates to a berberine abamectin aqua which is characterized in that abamectin and berberine plant extract are composed into the aqua, wherein, the content of the abamectin is 1mg / L to 2mg / L and the content of the berberine plant extract is 45mg / L to 55mg / L. The berberine abamectin aqua, by composing two kinds of pesticides with different mechanisms of action, not only can be favorable to solving the drug resistance of pests but also can improve the property of each drug, i.e. the content of the abamectin is greatly reduced, while the toxicity of the abamectin against the pests and the control of the berberine plant extract over the pests are improved; simultaneously, the aqua takes water as basic material, thus solving the problems of flammability and hazard caused by organic solvents and avoiding poisoning people and livestock and polluting environment.

The invention discloses pet shampoo and a preparation method thereof. The pet shampoo is prepared by adding antioxidant, a surface active agent, dimethyl silicone oil, pH modifier, preservative, a thickening agent, conditioner and spice with natural pyrethrin as raw materials. The preparation method includes the steps of weighing all raw materials according to the ratio and formula and sequentially adding the raw materials and conducting even mixing and stirring to obtain the target product, wherein the temperature in the even mixing and stirring process is controlled between 10 DEG C and 35 DEG C. The pet shampoo is excellent in medicated effect, easy to degrade, friendly to the environment and safe for warm-blooded animals, has an ideal pet parasite killing effect, and has a nursing effect on hair of pets.

The present invention relates to a method for improving beauveria bassiana conidiospore yield and virulence by use of genetic manipulation, a beauveria bassiana mutant strain can be obtained by destroying MIR structural domain encoded by beauveria bassiana Pmt1 gene and retaining completed PMT structural domain, and the beauveria bassiana mutant strain has improved conidiospore yield and virulence.