8results about How to "Increase virulence" patented technology

The invention relates to the technical field of solenopsis invicta prevention and control, in particular to metarhizium palustris for preventing and controlling solenopsis invicta as well as engineering bacteria and application of the metarhizium palustris. The Metarhizium anisopliae provided by the invention is a strain which is separated from the field and has high toxicity to the solenopsis invicta, and can be used for preventing and treating the solenopsis invicta or constructing engineering bacteria for preventing and treating the solenopsis invicta. The engineering bacterium constructed by the invention has high toxicity to solenopsis invicta, and can enable the bombyx batryticatus infected with the solenopsis invicta to volatilize a large amount of a solenopsis invicta attractant longifolene, so that the bombyx batryticatus has a trapping and killing effect on the worker solenopsis invicta, and the sanitary behavior of the worker is obviously slowed down. When the engineering bacteria constructed by the invention are combined with a small amount of chemical insecticide bait, the control effect of wettable powder taking spores of the engineering bacteria as an active component on a multi-ant nest (a single-ant nest) of solenopsis invicta is 79% (58%) within 3 days, 93% (90%) within 7 days and 98% (98%) within 28 days, and the method has the advantages of safety, high efficiency and the like.

The invention belongs to the technical field of rotifer killing, and particularly relates to a rotifer killing composition and a preparation method thereof. The composition is prepared by mixing a base liquid A, an emulsifier B and water. The base liquid A is prepared by mixing eugenol, patchouli alcohol, limonene, allicin, methyl salicylate, carvacrol and thujone in a mass ratio of 1-10:1-9:1-10:1-10:1-9:1-9:1-9; the B liquid is prepared by mixing the A liquid, tristyrylphenol polyoxyethylene ether, n-butanol and benzyl alcohol in a volume ratio of 1:8:2:0-4 in a reaction container at room temperature; the composition is prepared by adding deionized water to the B liquid and setting the volume to 100 ml. The composition uses herbaceous plants as preparation raw materials, can be directly sprinkled in waters contaminated by rotifers, can remove most harmful rotifers, and reduces pollution to the environment, and is environmentally friendly and practical.

The present invention relates to the technical field of pesticides, and relates to an efficient use method of Metarhizium reinhardtii Mr006, which is applied to the prevention and control of lepidopteran pests such as fall armyworm. Metarhizium reinhardtii Mr006 is mixed with chlorantraniliprole, and on the basis of clarifying the compatibility of the two, it is found that the treatment of first bacteria and then medicine, and first medicine and then bacteria have excellent control effects on fall armyworm, and the combination of the two has an excellent synergistic effect, achieving the purpose of improving the insecticidal activity of Metarhizium reinhardtii Mr006 and reducing the amount of chlorantraniliprole; at the same time, the effect of taking both quick-acting and lasting effects into consideration is achieved, providing ideas for pest resistance management, and providing a basis for long-term green control of fall armyworm populations.

This invention discloses a method for enhancing the ability of Trichoderma to control plant diseases, belonging to the field of biocontrol technology. This invention is the first to identify the rice black spore divirus NoPV1-COTG-19 carried by *Trichoderma guiyuan* COTG-19, and confirms that this virus can enhance the inhibitory ability of *Trichoderma* against plant pathogens. Plate confrontation experiments show that the virus-carrying COTG-19 strain has a significantly higher inhibition rate against *Pseudomonas aeruginosa* than the virus-free COTG-19-FV strain. Pot and field trials show that the NoPV1-COTG-19 strain has excellent control effects against *Tetracentron sinense* canker, and the NoPV1-COTG-19 virus can be horizontally transmitted to other *Trichoderma* strains, significantly enhancing the sporulation capacity of infected *Trichoderma* strains. This invention provides a novel technical approach to improving the biocontrol efficacy of *Trichoderma*, and has broad application prospects in the field of green control of plant diseases.

The invention provides dsRNA (double-stranded ribonucleic acid) of hyphantria cunea HcARNT and HcCncC genes, wherein the full-length cDNA of the HcARNT and the full-length cDNA of the HcCncC genes are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2. The dsHcARNT or the dsHcCncC can silence the expression of the HcARNT or the HcCncC of the fall webworm respectively, interfere the HcARNT gene or the HcCncC gene of the fall webworm, and can enhance the insecticidal effect of tannic acid which is a plant secondary substance. The invention also provides application of the dsRNA in prevention and treatment of fall webworms, and the timely scheme provided by the invention can reduce the use of pesticides, and has the beneficial effects of greenness, environmental protection and the like.

The present application provides a protein and its use in improving the virulence of a parasporal crystal protein of Bacillus thuringiensis, which comprises a protein having an amino acid sequence shown in SEQ ID NO: 1. The protein of the present application can effectively improve the virulence of the parasporal crystal protein, especially the insecticidal capacity, after cooperating with the parasporal crystal protein, thereby reducing the use amount of the parasporal crystal protein, lowering the insecticidal cost, improving the insecticidal efficiency, and being suitable for large-scale popularization and application.

The application discloses application of rifampicin in preparation of a Myzus persicae insecticide.

This invention provides applications of the pfaR and pfaK genes of *Aeromonas dacca*, belonging to the field of microbial technology. This invention is the first to identify a previously uncharacterized OmpR / PhoB family response regulator, pfaR, and hypothesizes that pfaR and pfaK constitute a functionally paired two-component system. Experiments show that pfaR can promote the virulence of *Aeromonas dacca* and directly regulate argF1 to promote its biofilm formation. This invention provides new molecular evidence for understanding the pathogenic regulatory network of *Aeromonas dacca* and lays a theoretical foundation for the development of antiviral drug targets.