Kit for detecting staphylococcus epidermidis

Abstract

The invention provides a kit for detecting staphylococcus epidermidis. The kit comprises a guide RNA specifically targeting a staphylococcus epidermidis rpoB gene, amplification primer pairs, hydratedTwistAmp basic kit reaction drying balls, an LbCas12a protein, a single-stranded DNA probe (ssDNA), a Ribonuclease Inhibitor and a buffer solution. The kit is used for detecting the staphylococcus epidermidis, the detection specificity is high, and the staphylococcus epidermidis can be distinguished from other staphylococcocci including staphylococcus aureus, staphylococcus hominis, staphylococcus warneri, staphylococcus capitis and staphylococcus haemolyticus. The RNA sequence is used for detection, the consumed time is about 1 hour, no PCR instrument is needed, the requirements for equipment are low and significantly lower than those of a traditional bacterial culture method or PCR sequencing method, and the clinical practical value is high.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for the detection of Staphylococcus epidermidis, comprising a guide RNA specifically targeting the rpoB gene of Staphylococcus epidermidis, a pair of RPA amplification primers, and a hydrated TwistAmp basickit for reaction drying Ball, LbCas12a protein, single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor and buffer. This kit can be used for rapid detection of Staphylococcus epidermidis. Background technique [0002] Staphylococcus epidermidis is a common bacterial group among Gram-positive bacteria that infects human beings, which brings a huge burden to patients and society. The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus epidermidis from other staphylococci due to the lack of specific primers, so joint generation sequencing is require...

AI Technical Summary

Problems solved by technology

The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Staphylococcus epidermidis from other staphylococci due to the lack of specific primers, so joint generation sequencing is required Identification, high cost, takes several days to get results

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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