46 results about "Osteomyelitis" patented technology

Described herein are methods and compositions for using microbial agents (probiotics) and agents that promote growth of certain microbes (prebiotics) for management (including prevention and treatment) of musculoskeletal disorders, including osteoporosis, osteopenia, Paget's disease, stunting, osteoarthritis, osteomyelitis, and delayed or non-union fractures. Also described herein are methods and compositions for using probiotics and prebiotics for management of inflammation, and symptoms of menopause.

The invention discloses an antibacterial peptide modified bionic nanoparticle targeting delivery system as well as a construction method and application thereof. The antibacterial peptide modified bionic nanoparticle targeting delivery system comprises a recombinant antibacterial peptide LL-37, a macrophage membrane and an enzyme cascade reaction system, the recombinant antibacterial peptide LL-37 is expressed on the macrophage cell membrane to form a macrophage cell membrane modified by the recombinant antibacterial peptide LL-37; the enzyme cascade reaction system is wrapped by the macrophage membrane modified by the recombinant antibacterial peptide LL-37, so that the bionic nanoparticle targeted delivery system modified by the antibacterial peptide is formed; wherein a Toll-like receptor 2 and a Toll-like receptor 4 are highly expressed on the surface of a macrophage cell membrane; the enzyme cascade reaction system comprises a nano enzyme with catalase-like activity and a hydrogen peroxide-producing biological enzyme. The antibacterial peptide modified bionic nanoparticle targeting delivery system disclosed by the invention can be used for treating infectious bone defects such as periodontitis, peri-implantitis, infectious fracture, osteomyelitis and the like.

The present invention discloses an antibacterial artificial nanocell and its preparation method and application. The antibacterial artificial nanocell is used for preparing a drug for treating osteomyelitis, and the antibacterial artificial nanocell is prepared through the following steps: mixing polyvinylpyrrolidone or its aqueous solution, CuCl2·2H2O or its aqueous solution, FeCl2·4H2O or its aqueous solution, elesclomol or its DMSO solution, NaOH or its aqueous solution, and H2O2 or its aqueous solution, stirring evenly, washing, ultrafiltering, and centrifuging to obtain nanoparticles; preparing cell membranes by using bone marrow stromal stem cells; mixing the nanoparticles and the cell membranes evenly, and incubating and extruding them by ultrasound to obtain the antibacterial artificial nanocell; wherein, the mass ratio of the protein of the nanoparticles to the cell membranes is 1:(0.9-1.1). The antibacterial artificial nanocell provided by the present invention effectively avoids the two major clinical problems of large side effects caused by long-term and high-dose use of antibiotics and high risk of debridement surgery in the traditional treatment of osteomyelitis, and kills bacteria from the perspectives of mediating Fenton reaction and cuproptosis, two non-antibiotic principles, so as to achieve excellent osteomyelitis treatment effect.

The present invention relates to the technical field of medical devices, and specifically to a flushing device and flushing method for intraoperative osteomyelitis. The device includes a carrying box, a bottom of one side of the carrying box is connected to a liquid outlet pipe, a side wall of the carrying box is connected to a suction pipe and a negative pressure component, a top of the carrying box is provided with a liquid storage component, the liquid storage component is connected to a flushing pipe, the flushing pipe is fixedly connected to a flushing head, the suction pipe is fixedly connected to a suction head, the suction head is wrapped around the outside of the flushing head, the flushing head and the suction head are fixedly connected, a plurality of suction holes are circumferentially provided on the side wall of the suction head, a flushing hole is provided on the flushing head, a plurality of anti-blocking holes are provided on the side wall of the flushing head, and a one-way liquid valve is fixedly connected in the anti-blocking holes. The method includes S1 selecting the negative pressure flushing intensity, S2 flushing and suction, and S3 adjusting the balance. The present invention combines the integration of flushing and suction, designs the structure, and uses the flushing liquid sprayed by flushing to clear the suction channel that is easily blocked, thereby improving the efficiency, safety and recovery speed of the operation.

The invention discloses a manganese-based layered hydroxide modified by gold nanoparticles and a preparation method thereof, and relates to the technical field of drug synthesis, and the key points of the technical scheme are as follows: the layered morphology of Mn-LDH is observed on the manganese-based layered hydroxide in a transmission electron microscope, and spherical AuNPs dispersed on the surface and edge of a laminate have a uniform particle size and a uniform particle size. EDS energy spectrum analysis finds that gold, manganese, magnesium, aluminum and carbon elements exist, the hydrated particle size is 312 nm, and the dispersity is good. The material can initiate an excellent photo-thermal effect under the irradiation of 808nm near-infrared light to generate active oxygen and hydroxyl radicals to destroy bacterial cell walls and cell membranes, and the antibacterial effect can reach 99%. In addition, the material can stimulate a local active immune function, promote T cell infiltration and realize active immune sterilization. The nano material shows a remarkable anti-infection effect in an in-vitro antibacterial experiment and an in-vivo osteomyelitis animal model experiment, and a new insight is provided for treating osteomyelitis caused by methicillin-resistant staphylococcus aureus.

The invention relates to the technical field of image registration analysis, and particularly discloses an osteomyelitis lesion analysis method based on medical image registration, which ensures the precision of registration information by providing a precise target area and ensures the accuracy of a lesion image evaluation result extracted after registration. The method comprises the following steps: step 1, carrying out a multimode registration simulation experiment on a reference image and a floating image based on a mutual information technology to obtain a registration target area; the reference image is a CT image, and the floating image is a CBCT image; step 2, carrying out test analysis after carrying out spatial transformation on the registration target area, and judging whether the actual mutual information value between the two images meets the requirement or not according to a test analysis result: if so, obtaining the information of the registration target area and extracting the feature of a lesion area; if not, returning to the step 1 to adjust the target area range of multimode registration; 3, performing statistical analysis on lesion area features to obtain lesion area images; and 4, analyzing the lesion area image and outputting an evaluation result.

Described herein are methods and compositions for using microbial agents (probiotics) and agents that promote growth of certain microbes (prebiotics) for management (including prevention and treatment) of musculoskeletal disorders, including osteoporosis, osteopenia, Paget's disease, stunting, osteoarthritis, osteomyelitis, and delayed or non-union fractures. Also described herein are methods and compositions for using probiotics and prebiotics for management of inflammation, and symptoms of menopause.

Enfumafungin derivative triterpenoid antifungal compounds are used to treat osteo-articular fungal infections, due to their unexpected bone tissue penetration and associated efficacy for such infections. The enfumafungin derivative triterpenoids (or pharmaceutically acceptable salts or hydrates thereof) are inhibitors of (1,3)-β-D-glucan synthesis and are useful in the treatment of yeast or mold infections that occur in osteo-articular structures such as osteomyelitis, spondylodiscitis, and arthritis.

The invention discloses a bone filling scaffold material composition for osteomyelitis and a preparation method thereof. The bone filling scaffold material composition comprises a first component and a second component, the first component comprises the following components in parts by mass: 1-5 parts of a cyclic monomer and 1-10 parts of a monomer containing double bonds; and the second component comprises the following components in parts by mass: 0.0011-12 parts of a metal ion compound. According to the bone filling scaffold material composition for osteomyelitis, provided by the invention, in a high ROS environment of excessive inflammation, metal ions and H2O2 are subjected to a Fenton reaction to generate hydroxyl free radicals with higher oxidation potential energy, and due to energy generated by the Fenton reaction and the action of the free radicals, polymer chains are crosslinked and entangled, so that the bone filling scaffold material composition for osteomyelitis is formed. Therefore, the mechanical property of the material is improved, and self-enhancement is realized; the monomer residual amount in the material is effectively reduced, and the biocompatibility of the material is improved.

The present application relates to the technical field of medical devices, mainly applied to patients with diabetic foot ulcers or osteomyelitis. Specifically, it is a kind of flushing drainage device, including support frame, the front bottom of support frame is provided with flushing drainage part, flushing drainage part includes two drainage components, the inner wall of two drainage components is provided with positioning assembly. The present application is provided with support frame and flushing drainage part, which realizes the significant improvement of the efficiency and safety of the operation of flushing and drainage of the deep cavity of the patient's bone, which is due to a carefully designed mechanical linkage structure. The structure can automatically complete the extension action of the spray pipe during the preparation stage of flushing, and ensure that the protection plate is quickly and stably closed, which not only ensures the accuracy of the flushing process, but also greatly reduces the risk of secondary injury to the patient during operation. Moreover, through the independent operation and cooperative mechanism of the left and right drainage components, the left component focuses on accurate flushing of the lesion area, and the right component is committed to efficient drainage.

Provided herein are pharmaceutical compositions, therapeutic combinations, devices and methods for treating, ameliorating, reversing, causing the remission of, and / or preventing (acting as a prophylaxis, or preventing the initiation of) an inflammatory bowel disorder (IBD) or inflammatory bowel disease (IBD), Ulcerative Colitis; Crohn's disease; J-pouch; fistulising Crohn's disease; a Colitis which can be microscopic, lymphocytic or collagenous; an eosinophilic colitis; indeterminate colitis; idiopathic colitis; diverticulosis and diverticulitis; relapsing diverticulitis; constipation associated inflammatory bowel disease and / or small intestinal bacterial overgrowth; Irritable Bowel Syndrome (IBS) with or without diarrhoea, constipation or pain predominant IBS; periodontitis; rheumatoid arthritis; respiratory infections, appendicitis, vascular disorders such as thrombophlebitis; bacteremia; osteomyelitis; septic shock; Alzheimer's disease; Lemierre syndrome (postanginal sepsis); colonic polyps or adenomas (optionally hyperplastic, adenomatous or serrated adenomas) or preventing the growth of colonic polyps or adenomas, bowl cancer, or metastases (optionally preventing the initiation or promotion of bowl cancer or metastasis); pharyngitis; otitis; sinusitis; and any disease, symptom or condition caused or exacerbated by a Fusobacteria (optionally, a F. nucleatum or F. varium) infection. In alternative embodiments, pharmaceutical compositions comprise rifaximin alone or in combination with other antibiotics or drugs.

This application provides an application of a Raman spectroscopy biomarker combination in the preparation of diagnostic products for diabetic foot osteomyelitis, belonging to the field of biomedical detection technology. The biomarker combination includes a first type of characteristic spectrum showing a significant increase in intensity or intensity ratio in positive samples of diabetic foot osteomyelitis compared to negative samples, and a second type of characteristic spectrum showing a significant decrease in intensity; specifically involving 1070 cm⁻¹. ‑1 With 1450 cm ‑1 The strength ratio, (851+873+917) cm ‑1 Region and 1656 cm ‑1 The strength ratio, and 960 cm ‑1 1001 cm ‑1 1103 cm ‑1 1450 cm ‑1 1590 cm ‑1 1656 cm ‑1 The intensity of multiple characteristic wavelengths was measured. The diagnostic model constructed based on this combination demonstrated good discrimination performance in testing. The method provided in this application has the advantages of being rapid and low-cost, offering a new solution for the early screening and auxiliary diagnosis of diabetic foot osteomyelitis.

Compositions for identifying a pathogenic bacterial infection include a mannitol compound with one or more substituents including radioisotopes. These radiopharmaceuticals, such as a positron-emitting mannitol analogue, [18F]fluoromannitol ([18F]FMtl), are rapidly taken up by gram-positive and gram-negative bacteria such as E. coli, S. aureus, A. baumannii, S. epidermis, P. mirabilis, S. enterica, K. pneumonia, E. faecium, E. cloacae, and M. marinum, but not non-active infection sites such as sterile inflammatory sites, cancer sites, etc. Administration of these radiopharmaceuticals to and subsequent imaging of patients, e.g., via positron emission tomography (PET), enables detection of deep-seated and difficult to manage bacterial infections, such as osteomyelitis and prosthetic joint infection, or in patients with sickle cell disease. [18F]FMtl injection detects and differentiates infection rapidly. The radiolabeled mannitol compounds can be produced via nucleophilic substitution reactions that are deployable on commercially available synthesizers, facilitating straightforward and wide accessibility, and counteracting unnecessary antibiotic use.

The invention relates to the technical field of biomedicine, and discloses an experimental method for treating osteomyelitis by using human umbilical cord mesenchymal stem cells. The method aims at solving the problems that an existing osteomyelitis experimental model is disjointed from clinical pathological characteristics, the activity loss of mesenchymal stem cells in an infection environment is large, a combined intervention scheme lacks pathological suitability, and an evaluation system is single. The method comprises the following steps: firstly, constructing a clinical adaptive model by combining pathogenic bacterium injection with immunosuppression and ischemia induction, then carrying out anti-oxidation and targeted colonization double pretreatment on MSC, then implementing stepped combined intervention according to the requirements of acute-stage bacterium control and chronic-stage repair, and comprehensively evaluating through infection control, immune balance and bone repair multi-dimensional indexes, so as to obtain a bone repair model. The constructed model is closer to clinical pathological characteristics, the MSC activity and colonization ability are stable, the intervention scheme collaboration is high, the evaluation result is comprehensive and reliable, a standardized tool can be provided for preclinical research of osteomyelitis treatment schemes, and the conversion guidance value of the experimental result is improved.

The present application relates to the technical field of external use of traditional Chinese medicine, in particular to an external use synthetic traditional Chinese medicine composition for treating osteomyelitis, sinus tract and stubborn ulcer and a preparation method thereof, which comprises, by weight, 10-15 parts of honeysuckle, 10-15 parts of dandelion, 10-12 parts of purple flower ground ivy, 6-10 parts of Chinese cork tree, 6-10 parts of bitter apricot seed, 5-8 parts of red root of sophora, 5-8 parts of Chinese angelica, and 1-4 parts of borneol; the present application is applicable to the infection period and the repair period, realizes the integration of anti-inflammatory, bacteriostasis, muscle regeneration and repair, adopts low-temperature ultrasonic extraction, avoids high-temperature damage to heat-sensitive components, has stronger and more stable efficacy, the formula contains blood-activating and stasis-removing components such as red root of sophora, Chinese angelica, frankincense and myrrh, can promote the penetration of the medicine into deep tissues, has a remarkable effect on the sinus tract and osteomyelitis wound, is a pure traditional Chinese medicine for external use, does not add hormones and antibiotics, is mild in nature, and is suitable for long-term use.

Systems, devices and methods for controlled intramedullary delivery of light (frequencies from about 380 nm to about 500 nm) to treat tissue or bones disorders, including osteomyelitis, by a flexible fiber are provided, where the light is delivered in a circumferential fashion around the fiber, and where the energy delivered from the fiber is of a similar average intensity at the front end and back end of the fiber, and in between. The methods and systems deliver intramedullary light to the canal over long lengths via a minimally invasive pathway to a bone. The methods and systems deliver and maintain a light delivery system within the canal of the bone to provide single or multiple doses of light to kill, eliminate, remove or reduce bacteria, viruses, fungus and pathogens, without removal of the light fiber system, thereby providing single or multiple treatments.

The invention discloses a perfusate suspension device with dual-redundancy pressure protection, relates to the technical field of lavage after tibial osteomyelitis in the children orthopedics department, and aims to solve the problem that an existing perfusate suspension device is difficult to operate in lavage after tibial osteomyelitis in children. The technical problems that in the prior art, a dynamic pressure protection mechanism matched with child bone growth is lacked, interference of temperature on pressure monitoring is not eliminated, redundant protection is not achieved, and bone wall damage or incomplete lavage is likely to happen are solved, and the device comprises a hanging assembly used for bearing a perfusate container; the perfusion assembly comprises a perfusion pipeline and a lavage needle, and the two ends of the perfusion pipeline are connected with an outlet of the perfusate container and the lavage needle respectively; and the dual-redundancy pressure monitoring module comprises two independent pressure acquisition paths which are used for respectively acquiring the pressure in the marrow cavity and the pressure in the perfusion pipeline, and the two acquisition paths are synchronously connected to the temperature compensation unit. The device has the advantages that accurate pressure monitoring and dynamic threshold adjustment are achieved, and the child lavage safety and effect are guaranteed.

Systems, devices and methods for controlled intramedullary delivery of light (frequencies from about 380 nm to about 500 nm) to treat tissue or bones disorders, including osteomyelitis, by a flexible fiber are provided, where the light is delivered in a circumferential fashion around the fiber, and where the energy delivered from the fiber is of a similar average intensity at the front end and back end of the fiber, and in between. The methods and systems deliver intramedullary light to the canal over long lengths via a minimally invasive pathway to a bone. The methods and systems deliver and maintain a light delivery system within the canal of the bone to provide single or multiple doses of light to kill, eliminate, remove or reduce bacteria, viruses, fungus and pathogens, without removal of the light fiber system, thereby providing single or multiple treatments.

A procedure for the treatment of osteomyelitis in long bones comprising the steps of: - approaching the bone canal of a long bone either at its distal or proximal end through a wound made on a patient; - reaming said bone canal to a maximum diameter of approximately 12 mm (0.47 in) without affecting the cortical layer; - taking samples for culture; - cleaning and aspirating the contents of said bone canal; - inserting into said bone canal, a multi-perforated plastic cannula (1) with holes (2) therein by means of a metal guide (3) arranged inside and having the exterior thereof covered with a sponge (4); - removing said metal guide (3); - arranging an additional portion of sponge in said wound in such a way that one portion is placed deep in contact with said a multi-perforated plastic cannula (1) coated with a sponge (4), and another portion emerges from said wound; - partially closing said wound with sutures; - fixing a self-adherent absorption disc that includes an aspiration hose upon said additional portion of sponge emerging from said wound; - coating said wound with a self-adherent film to form a complete seal; - connecting by means of a connector said aspiration hose to a collection hose which is connected to an external graduated container; - connecting said external graduated container to a suction pump; - turning on said suction pump; - absorbing and transferring the fluid generated within said bone canal continuously to said external graduated container; - measuring the aspirated fluid; - carrying out the above steps three times every 48 hours; and - after the third time, completely removing said a multi-perforated plastic cannula covered with said sponge from said wound by re-reaming, washing, and taking cultures for laboratory testing. This invention also discloses a device for the treatment of osteomyelitis in long bones used in the procedure.

The present application relates to a kind of bone-targeted trimeric protein and its preparation method and application, belong to biomedical technology field.The present application provides a kind of recombinant trimeric protein based on trimeric motif, including nuclear factor κB receptor activation factor extracellular segment, it is verified with in-vivo and in-vitro experiment with high bone targeting, possess inhibiting osteoclastogenesis and promoting osteoblast differentiation Dual control activity, it can also induce CD4 + Treg cell development, improve the bone marrow inflammatory microenvironment of osteoporosis model mice, can be used as the drug of osteoporosis, solves the problem that traditional single-function treatment drug in prior art will cause bone resorption rebound and bone loss after stopping drug, and further induces inflammation, provides new ideas and means for the research and treatment of osteoporosis, has good application prospect.

The invention provides a recurrence risk assessment method for treating osteomyelitis through an induced membrane technology, and belongs to the technical field of disease assessment. The method comprises the steps of S1, multi-center data and queue design; s2, two high-annuity radiologists sketch ICCgt layer by layer by adopting a blind method; if 0.80, the ROI is locked; a difference gt; when 0.80, third-person arbitration is introduced; s3, high-dimensional feature extraction and refining; s4, machine learning modeling is carried out; and S5, interpretable output is carried out. According to the method, the scoring objectivity and consistency are remarkably improved; the change of the internal microstructure of the induction film is quantified in vivo and noninvasively for the first time; individualized operation opportunity adjustment can be achieved through longitudinal monitoring, and a result can be explained and can directly guide treatment decisions.

The invention discloses an intramedullary nail capable of slowly releasing antibiotics. The intramedullary nail comprises an intramedullary nail body, a medicine cavity is formed in a rod body of the intramedullary nail body, a plurality of microspheres are put into the medicine cavity, the antibiotics are wrapped in the microspheres, and a plurality of releasing micropores are formed in the rod body, corresponding to the medicine cavity, of the intramedullary nail body. According to the intramedullary nail, the microspheres sealed with antibiotics can be put into the intramedullary nail, the antibiotics can be slowly released through the release micropores, the anti-infection effect on osteomyelitis and local infection patients can be achieved, the microspheres do not need to be taken out in a short time, the pain of the patients is relieved, and the intramedullary nail has the advantages of being simple in structure, convenient to use, low in cost and the like.

The application discloses PtCu@BSA nanoparticles and a preparation method and application thereof, and relates to the technical field of nanometer materials.The PtCu@BSA nanoparticles take porous PtCu-based nanoparticles as a framework, and are wrapped with BSA on the surface, which is favorable for enhancing the biocompatibility and stability of the PtCu@BSA nanoparticles; meanwhile, the porous PtCu-based nanoparticles have high targeting capability and electromotive catalytic effect, can well catalyze water decomposition into hydroxyl radicals under the action of an alternating electric field, thereby effectively killing bacteria, and improving the limitation that antibiotics are frequently used in existing drugs for treating osteomyelitis.Meanwhile, in the process of electromotive catalysis, the porous PtCu-based nanoparticles are partially degraded into positively charged ions such as Pt 2+ , Pt 4+ , Cu 2+ , and the ions have strong biofilm penetration, and the ions can synergistically regulate an infected microenvironment, so that local inflammation is subsided.

The invention relates to a drug-loaded sodium alginate hydrogel antibacterial stent based on halloysite nanotubes as well as a preparation method and application of the drug-loaded sodium alginate hydrogel antibacterial stent, and belongs to the technical field of material synthesis and biological medicines. The stent is a stent with a certain porosity and a certain aperture structure, which is obtained by mixing a drug-loaded halloysite nanotube, sodium alginate and methyl cellulose and sequentially performing 3D printing and crosslinking, and the drug-loaded halloysite nanotube is embedded in sodium alginate / methyl cellulose hydrogel. The drug-loaded halloysite nanotube is obtained by mixing a halloysite nanotube modified by polyethyleneimine and a broad-spectrum antibacterial agent with negative electricity. The stent has the characteristics of controllable degradation rate and biocompatibility, and is suitable for local anti-infection and bone regeneration treatment after osteomyelitis debridement.

The invention provides a composite scaffold material and a preparation method and application thereof, and relates to the technical field of composites.The preparation method comprises the steps that ferric chloride hexahydrate and anhydrous sodium acetate are added into ethylene glycol for a reaction, and Fe3O4 nanoparticles are obtained; potassium ferricyanide and a dispersion medium are added into a hydrochloric acid solution to be dissolved, then Fe3O4 nanoparticles are added for a reaction, and a Fe3O4 / PB heterojunction is obtained; the preparation method comprises the following steps: mixing and grinding a Fe3O4 / PB heterojunction and poly-L-lactic acid, and carrying out laser printing to form the PLLA-Fe3O4 / PB microwave antibacterial composite scaffold material. PLLA is taken as a carrier, the Fe3O4 / PB heterostructure scaffold material is loaded, the microwave absorption performance is enhanced through a magnetic-dielectric synergistic effect, under microwave irradiation, by virtue of a dual mechanism of interface polarization and dipole polarization, the composite scaffold material is prepared into the PLLA-Fe3O4 / PB microwave antibacterial composite scaffold material, and the composite scaffold material is prepared into the PLLA-Fe3O4 / PB microwave antibacterial composite scaffold material. Microwave thermal therapy and microwave power are synchronously realized, and the microwave thermal therapy and the microwave power synergistically show an efficient antibacterial effect on the osteomyelitis infected by the staphylococcus aureus.

The invention relates to the technical field of biomedical materials, in particular to carbon dot hydrogel with antibacterial, anti-inflammatory and osteogenesis promoting functions as well as a preparation method and application of the carbon dot hydrogel. The preparation method comprises the following steps: performing hydrothermal reaction on o-phenylenediamine, caffeic acid and selenium tetrachloride to prepare selenium-doped carbon dots Cat-SeCD; phenylboronic acid functionalized sodium alginate PBA-SA and a catechol structure on the surface of the Cat-SeCD are cross-linked through a dynamic covalent bond to form gel. According to the application, the carbon dots can be used as active centers and cross-linking points, and on-demand release of the Cat-SeCD can be realized in an acidic or high oxidative stress microenvironment of an osteomyelitis focus. The Cat-SeCD integrates multiple functions of broad-spectrum antibiosis, free radical scavenging, bone marrow mesenchymal stem cell osteogenic differentiation promotion and the like, realizes three-in-one synergistic intervention of sterilization, anti-inflammation and osteogenesis promotion, and has excellent biocompatibility and clinical application potential.

The invention discloses novel fusidic acid crystal forms as well as a preparation method and application thereof. The novel fusidic acid crystal forms are NY01 and NY02. The fusidic acid new crystal form NY01 or NY02 provided by the invention can be prepared into preparation forms such as capsules, tablets, emulsifiable paste, gel and the like with other pharmaceutically acceptable auxiliary materials so as to adapt to different administration routes; the pharmaceutical composition is used for treating osteomyelitis, septicemia, endocarditis, pneumonia, cystic fibrosis, skin and soft tissue infection, surgical operation infection or trauma infection caused by sensitive microorganisms, especially staphylococcus.

The invention discloses a periosteum distraction device. The periosteum distraction device comprises a distraction plate, at least one barrier plate, a distraction screw and a steel needle. A threaded hole and a cylindrical fixing hole I are formed in the center of the distraction plate; the blocking plate is located below the stretching plate, and a center hole and a second cylindrical fixing hole are formed in the center of the blocking plate. The distraction screw is a hollow screw and is used for being screwed into the threaded hole along the steel needle; and the steel needle is used for penetrating through the central hole and drilling into sclerotin to be fixed. The diameter of the center hole is smaller than the outer diameter of the distraction screw, and a steel needle can pass through the center hole. The blocking plate is arranged below the distraction plate, the distraction screws are effectively prevented from penetrating into bone cortex in the distraction process, the risks of bone destruction, fracture and osteomyelitis are reduced, meanwhile, local pain in the distraction process is relieved, and the periosteum distraction device is suitable for periosteum distraction treatment of osteoporosis patients.

The present disclosure provides methods for detecting septic arthritis, transient synovitis, or osteomyelitis, based on protein signatures. Specifically, the method comprising: (i) measuring expression levels of one or more proteins in a biological sample obtained from the subject; (ii) determining a protein signature correlated with a detected disease based on the expression levels of the proteins in step (i); and (iii) assessing the occurrence or severity of the subject correlated with the disease based on the protein signature determined in step (ii). The methods may further comprise identifying suitable treatment for the patient based on the protein signatures.

The invention is applicable to the technical field of biological medicine, and provides application of hesperidin in preparation of a medicine for treating staphylococcus aureus osteomyelitis, hesperidin serves as a novel medicine for inhibiting staphylococcus aureus virulence regulatory factors, and activates Nrf2 / SLC7A11 / GPX4 signal channels in osteoblasts by inhibiting activity of the staphylococcus aureus virulence regulatory factors AgrA and SarA, so that the activity of the staphylococcus aureus virulence regulatory factors AgrA and SarA is inhibited, and the activity of the staphylococcus aureus virulence regulatory factors AgrA and SarA is inhibited. The composition can inhibit osteoblast ferroptosis, alleviate inhibition of staphylococcus aureus on cell viability, differentiation and mineralization of osteoblasts, reduce cortical bone destruction and bone mass loss of mice with staphylococcus aureus osteomyelitis, and has a good protection effect on staphylococcus aureus osteomyelitis.