36 results about "Bone targeting" patented technology

The invention relates to the technical field of biomedical materials, in particular to an alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material and a preparation method and application thereof.The alendronate-modified ZIF-8 serves as a carrier, TPP-modified carbon dots are encapsulated in the carrier, citric acid, urea and cerium salt serve as raw materials of the TPP-modified carbon dots, and the alendronate-modified ZIF-8 encapsulated TPP-carbon dot bone targeting nano-material is prepared through a one-step method. After cerium-doped carbon quantum dots are synthesized through a hydrothermal method, TPP is grafted through an amide method, alendronate endows the material with a bone targeting function, and TPP modified carbon dots endow the material with antioxidant and mitochondrial targeting functions. According to the invention, the bone targeting property is excellent, and bone focuses are efficiently enriched; the anti-oxidation and anti-inflammatory capacities are high, and the infected microenvironment can be improved; zIF-8 packaging is stable, and functional components can be controllably released; multiple components cooperate to promote bone repair, accumulation of non-target organs is little, and the composition is safe, reliable and suitable for treatment of various bone-related diseases.

The invention relates to the technical field of preparation of bone cement, and particularly discloses a preparation method of a photo-thermal response type gold nanorod-bone cement composite material. The method comprises the following steps: synthesizing a gold seed solution; constructing a growth solution; controlling the growth of the nanorod; gradient centrifugal purification; carrying out surface ligand exchange (sulfydryl-PEG); preparing a bone cement composite material; curing and forming; gold nanorods (GNRs) are uniformly embedded into a polymethyl methacrylate (PMMA) bone cement matrix, so that the problems that free nanorods are easy to migrate and poor in bone targeting are solved. The local temperature can be accurately controlled, and damage to surrounding tissues is avoided. According to the invention, immunogen cell death (ICD) can be effectively induced, and anti-tumor immune response can be activated. According to the invention, enough mechanical support can be provided, and the stability after implantation is ensured.

The application belongs to the technical field of bioactive nanomaterials, and particularly relates to a metformin nanomaterial, a preparation method and application thereof. The average hydrodynamic diameter of the nanomaterial is less than 500 nm, and the nanomaterial is prepared by a preparation method comprising the following steps: self-assembly of metformin and polyphenols to obtain metformin-polyphenol nanoparticles; reaction of bisphosphonate and polyethylene glycol to obtain bisphosphonate-polyethylene glycol organic segments; and reaction of the metformin-polyphenol nanoparticles and the bisphosphonate-polyethylene glycol organic segments to obtain the nanomaterial. The metformin, polyphenol, polyethylene glycol and bisphosphonate are prepared into the metformin-loaded nanomaterial through specific reactions, the systemic delivery of metformin is realized, the efficacy and bioavailability of metformin on osteoporosis are improved, and the bone targeting of metformin is also improved.

The invention provides a bone targeting nano composite carrier, a bone targeting nano composite drug and a preparation method and application of the bone targeting nano composite carrier and the bone targeting nano composite drug, and belongs to the technical field of biological medicine, the bone targeting nano composite carrier comprises epigallocatechin gallate and E7 targeting peptide, and the mass ratio of the epigallocatechin gallate to the E7 targeting peptide is 1: (1.5-2.5); the epigallocatechin gallate and the E7 targeting peptide are connected through a condensation reaction. According to the invention, the bone-targeted nano composite carrier is used for entrapping an osteogenic induction component, and a bone-targeted nano composite drug is obtained through self-assembly. According to the invention, triple functions of osteogenesis promoting drugs, anti-inflammatory / anti-oxidation components and active targeting are integrated, and the bone-targeting nano composite drug not only can directly stimulate bone formation, but also can fundamentally improve the crucial pathological microenvironment in diabetic osteoporosis.

The invention discloses a bone targeting forsythiaside A loaded exosome and a preparation method and application thereof, and relates to the technical field of biomedicine.The preparation method comprises the following steps that bone marrow mesenchymal stem cells are separated and cultured, and GLG1 is over-expressed through a molecular cloning technology to obtain GLG1-BMSCs; the exosome GLG1-Exos of the GLG1-BMSCs is collected through an ultracentrifugation method; and loading forsythiaside A into the exosome through an ultrasonic method and an extrusion method to obtain the GLG1-Exos-FTA. The traditional Chinese medicine active ingredient forsythiaside A is loaded, and forsythiaside A is delivered to bone marrow in a targeted manner, so that the effect of preventing and improving diabetic osteoporosis is achieved; due to the targeting effect, the bioavailability of forsythiaside A can be enhanced, and meanwhile, the curative effect of resisting diabetic osteoporosis is improved. Experiments show that the loading rate of forsythiaside A of the GLG1-Exos-FTA reaches 36.9%, the particle size distribution peak value is 122.4 nm, and the GLG1-Exos-FTA has typical exosome characteristics and bone targeting, and can significantly improve the bone microstructure of diabetic osteoporosis mice.

The invention discloses a bone targeting exosome polypeptide delivery system based on a biological orthogonal strategy and a preparation method and application thereof, and relates to the technical field of biological medicine and drug delivery. The bone targeting exosome polypeptide delivery system based on the biological orthogonal strategy comprises an engineered exosome as a carrier and an osteogenesis-promoting therapeutic polypeptide, wherein the osteogenesis-promoting therapeutic polypeptide is anchored on the membrane surface of the engineered exosome; a fusion protein is displayed on the membrane surface of the engineered exosome, and the fusion protein is formed by fusing a bone targeting ligand and an industrial exosome transmembrane scaffold protein; the osteogenesis-promoting therapeutic polypeptide is connected with the industrial exosome through the flexible spacer arm, and the membrane surface steric hindrance is reduced through the flexible spacer arm so as to improve the accessibility of the active fragment of the polypeptide; the delivery system provided by the invention can improve the targeted enrichment and treatment efficiency of osteogenesis promoting drugs in bone tissues, and is suitable for intervention of bone metabolism related diseases such as osteoporosis, delayed fracture healing, bone defects and the like.

The invention belongs to the technical field of biological medicine, and relates to a bone targeted drug liposome as well as a preparation method and application thereof, the bone targeted drug liposome is prepared from the following raw materials: phospholipid, cholesterol, PEG (polyethylene glycol), a targeted group and a drug; the phospholipid and the cholesterol form a lipid core layer, the PEG of which the tail end is coupled with the targeting group is connected to the surface of the lipid core layer, and the drug is encapsulated in the lipid core layer. The delivery efficiency of the drug is remarkably improved through modification of the targeting group, the solubility and stability of the drug can be improved through the liposome, the slow release effect is achieved, and the drug effect persistence is improved.

The invention belongs to the technical field of bioactive nano materials, and particularly relates to a metformin nano material as well as a preparation method and application thereof. The average hydrodynamic diameter of the nano material is less than 500 nm, and the nano material is prepared by the preparation method comprising the following steps: self-assembling metformin and a polyphenol compound to obtain metformin-polyphenol nano particles; the preparation method comprises the following steps: reacting bisphosphonate with polyethylene glycol to obtain a bisphosphonate-polyethylene glycol organic chain segment; the metformin-polyphenol nano particles and the diphosphonate-polyethylene glycol organic chain segment are subjected to a reaction, and the nano material is obtained. According to the invention, the metformin, the polyphenol, the polyethylene glycol and the bisphosphonate are subjected to a specific reaction to prepare the metformin-loaded nano material, so that the whole-body delivery of the metformin can be realized, the curative effect and bioavailability of the metformin on osteoporosis are improved, and meanwhile, the bone targeting property of the metformin is improved.

The invention discloses a naringin carbon quantum dot-based bone-targeting nanocomposite as well as a preparation method and application thereof. The preparation method comprises the following steps: 1, preparing naringin-derived carbon quantum dots (N-CQDs); 2, preparing hybrid silicon dioxide / hydroxyapatite nano particles MH; (3) preparing amino functionalized MH nano particles MH-NH2; 4, adding the activated N-CQDs solution into the MH-NH2 solution, and carrying out stirring reaction at room temperature to prepare MH-CQDs; and 5, dispersing the NHS-PEG-Mal into the MH-CQDs solution, slowly stirring, and dialyzing after the reaction is completed, so as to obtain the P-coated MH-CQDs. According to the nano-composite disclosed by the invention, a natural drug naringin with osteogenic activity is creatively converted into N-CQDs with a pharmacological function and a fluorescent tracing capability, and the N-CQDs are combined with a bionic silicon dioxide-hydroxyapatite substrate, so that the essential fusion of a'drug-probe 'function is realized.

The invention discloses a preparation method and an application of a bone targeting catechin-strontium metal polyphenol nano particle. The system takes a metal-polyphenol network (MPN) formed by coordination self-assembly of strontium ions and catechin as a core, and tetracycline is modified on the surface of the MPN to construct a bone targeting functional layer. The MPN structure can significantly enhance the loading efficiency and physiological stability of catechin, and the tetracycline modification on the surface endows the nanoparticles with excellent bone targeting ability. When reaching an osteoporosis focus, the nanoparticles can respond to a local weakly acidic microenvironment and controllably release catechin, and exert antioxidant and anti-inflammatory effects, thereby reversing a pathological microenvironment unfavorable for bone regeneration. Meanwhile, the MPN skeleton is gradually degraded in vivo, and the released Sr < 2 + > can promote proliferation and differentiation of pre-osteogenic cells, inhibit the activity of osteoclasts and promote formation of new bones. The system integrates bone targeting, microenvironment response drug release, immunoregulation and ion regulation treatment, and provides a new strategy for precise treatment of osteoporosis.

The application discloses a bone-targeting protein conjugated chelating agent and a preparation method and application thereof, and belongs to the technical field of biological medicines.The bone-targeting protein conjugated chelating agent is constructed through gene engineering and chemical conjugation technology, the chelating agent is covalently connected by a bone-targeting fusion protein and a small molecule chelating agent, the bone-targeting fusion protein part is composed of a bone-targeting peptide, a cell-penetrating peptide and a Halo tag protein, and the protein molecule can efficiently pass through the cell membrane to reach the bone tissue; the small molecule chelating agent is isothiocyanobenzyl DTPA, and can efficiently undergo nucleophilic addition reaction with lysine residues of the bone-targeting fusion protein under weak alkaline conditions to generate the bone-targeting protein conjugated chelating agent.The chelating agent has the targeting ability to the bone tissue, can be complexed with heavy metal ions deposited in the bone, and realizes precise chelation of the heavy metal ions deposited in the bone.

This invention belongs to the field of biomedical materials technology and discloses a highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability. The nanoparticle comprises zinc oxide nanoparticles with active sites and a bone-targeting material grafted onto the surface of the zinc oxide nanoparticles via the active sites. The active site is an amino group; the bone-targeting material is one of aspartic oligopeptide and glutamic acid oligopeptide. This invention also discloses the preparation method and application of the aforementioned highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability. The highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability provided by this invention can be directed to the site of bone infection through the targeting ability of the bone-targeting material for hydroxyapatite in bone tissue, and then exert the broad-spectrum antibacterial ability of the zinc oxide nanoparticles to kill bacteria.

The invention discloses a bone targeting protein coupling chelating agent as well as a preparation method and application thereof. Belongs to the technical field of biological medicine. The bone targeting protein coupling chelating agent is constructed through genetic engineering and chemical coupling technologies, the chelating agent is formed by covalent linkage of bone targeting fusion protein and a small molecule chelating agent, the bone targeting fusion protein part is composed of bone targeting peptide, cell-penetrating peptide and Halo tag protein, and the small molecule chelating agent part is composed of bone targeting peptide, cell-penetrating peptide and Halo tag protein. It is ensured that protein molecules can efficiently penetrate through cell membranes to reach bone tissues; the small molecule chelating agent is benzyl isothiocyanate DTPA, and can be subjected to efficient nucleophilic addition reaction with lysine residues of bone targeting fusion protein under a weak base condition to generate the bone targeting protein coupling chelating agent. The chelating agent not only has the targeting ability to bone tissues, but also can be complexed with the bone deposited heavy metal ions, so that the precise excretion promotion of the bone deposited heavy metal ions is realized.

The present application discloses a bone-targeting radiopharmaceutical or imaging agent and a use thereof. The bone-targeting radiopharmaceutical or imaging agent can be used for the treatment or diagnosis of bone diseases, such as bone metastasis from a tumor.

The application discloses a preparation method and application of a nano-enzyme composite hydrogel system with synergistic effects of anti-inflammatory, antioxidant and pro-osteogenesis. The material is based on an injectable hydrogel composed of methacrylated chitosan and thiolated sodium alginate, loaded with cerium nano-enzymes, and coated with carbon dots of baicalin and alendronate sodium in it through coordination. In a high oxidative stress microenvironment, the thioether bond in the hydrogel responds to reactive oxygen species and releases nanoparticles. Cerium nano-enzymes remove excess reactive oxygen species through their superoxide dismutase and catalase activities and continuously produce oxygen, synergize with the antioxidant and anti-inflammatory effects of baicalin in the carbon dots and the bone targeting effect of alendronate sodium, jointly regulate macrophage polarization to reshape the immune microenvironment, and promote osteogenic differentiation and angiogenesis. Therefore, the application provides a systematic treatment platform with the functions of active oxygen response release and multi-target regulation for the repair of diabetic alveolar bone defects, which is coupled with pathological microenvironment remodeling and bone regeneration.

The invention discloses a responsive nano-enzyme for delivering KGN and a preparation method and application thereof, and the preparation method comprises the following steps: suspending zinc chloride and cerium nitrate in an N, N-dimethylformamide solution, and suspending meso-tetra (4-carboxyphenyl) porphin in the N, N-dimethylformamide solution; adding a proper amount of glacial acetic acid; the preparation method comprises the following steps: dissolving tannic acid in an N, N-dimethylformamide solution, and dissolving meso-tetra (4-carboxyl phenyl) porphin in the N, N-dimethylformamide solution to obtain meso-tetra (4-carboxyl phenyl) porphin-Zn < 2 + >-Ce < 3 + >; the preparation method comprises the following steps: adding modified mercaptoacetic acid-meso-tetra (4-carboxyl phenyl) porphin-Zn < 2 + >-Ce < 3 + > and cysteine modified CAP bone targeting peptide into triethylamine, and reacting to obtain meso-tetra (4-carboxyl phenyl) porphin-Zn < 2 + >-Ce < 3 + >-tannic acid-coated CAP peptide with targeting property; the preparation method comprises the following steps: dissolving KGN in dimethyl sulfoxide, diluting, and adding into an N, N-dimethylformamide solution of SH-TCPP-Zn < 2 + >-Ce < 3 + > (at) TA to obtain the sulfydryl-tetra (4-carboxylphenyl) porphin-Zn < 2 + >-Ce < 3 + > (at) tannic acid (at) KGN(at) CAP peptide. The invention relates to a biodegradable nano particle with a cartilage targeting function.

The application discloses a kind of silicon dioxide-engineered extracellular vesicle composite nano-preparation for tendon-bone interface repair and its preparation method and application, the preparation includes carrier matrix and functional load component, functional load component is loaded in carrier matrix by the synergistic effect of physical adsorption and channel embedding;The carrier matrix is the bioactive silicon dioxide nanoparticle of core-shell structure, the mesoporous silicon dioxide with central radial channel is in the core, the shell is calcium phosphorus layer, and the nanoparticle surface is modified by bone targeting molecule;Functional load component is the engineered extracellular vesicle of bone marrow mesenchymal stem cell source preprocessed by the bioactive molecule with anti-inflammatory osteogenesis effect.The application is by the dual delivery strategy of " carrier + excretion component", on the one hand, active ion released by silicon dioxide carrier promotes osteogenesis, on the other hand, " high efficiency" exosome is stimulated, and local inflammatory microenvironment is adjusted, and the functional regeneration of tendon-bone interface is accelerated by synergistic effect.

The invention provides a cathepsin K (CTSK) responsive polypeptide derivative and application thereof. The CTSK responsive polypeptide derivative provided by the invention has the following structure: Ar-L1-dipeptide linker-hydrophobic membrane permeation sequence-CTSK response sequence-bone targeting sequence, and can be self-assembled to form bone targeting nanoparticles. According to the CTSK responsive polypeptide derivative and the bone targeting nanoparticles obtained from the CTSK responsive polypeptide derivative disclosed by the invention, the combination of osteoclast and apoptotic body mediated bone regeneration is specifically eliminated by utilizing self-assembly triggered by CTSK, so that bone balance is recovered, and physiological bone remodeling is not destroyed.

The invention discloses an application of a calcium-reinforced layered network structure emulsion in preparation of a product for promoting calcium absorption and enhancing bone mineral density, and aims to solve the technical bottlenecks of low absorption rate and insufficient bone targeting of a traditional calcium supplement. According to the emulsion, the insoluble calcium salt is uniformly dispersed in an oil phase by utilizing the density difference between the insoluble calcium salt and the oil phase, and is embedded into a layered network structure formed by proteoglycan. Hydrophobic molecules or hydrophobic chain segments spontaneously gather to form a hydrophobic region, and free diffusion of water molecules and water-soluble ions is effectively prevented, so that solubilization and stable dispersion of insoluble calcium salt are realized. The obtained emulsion has good processing stability and tolerance, the absorption rate of calcium is remarkably improved, and the bone mineral density is favorably enhanced. The system can be used as a calcium fortifying preparation to realize on-line addition, the process is simple and convenient, the system is suitable for various foods such as dairy products, biscuits, ice cream and plant milk, and preparation and application of high-calcium nutritious foods are realized.

This invention provides a pharmaceutical composition for promoting fracture healing, its preparation method, and its application. The composition, by weight, comprises 25-50 parts of an active core component, 8-25 parts of an osteogenic synergistic bioactive component, 3-15 parts of a bone-targeting transdermal penetration enhancer, and 5-15 parts of a moisturizing dispersant. The active core component is based on bone-healing traditional Chinese medicines such as calcined natural copper nanoparticles and Eupolyphaga sinensis polypeptides. The osteogenic synergistic component is based on collagen peptides of specific molecular weights and graded sodium hyaluronate. The bone-targeting transdermal penetration enhancer is a compound of eugenol and limonene. This invention utilizes a three-dimensional synergistic formulation structure and a phase-separated low-temperature preparation process, overcoming the shortcomings of traditional topical medications, such as poor transdermal targeting, weak efficacy, easy inactivation, and low safety. It can achieve targeted enrichment at fracture ends, synergistically promoting fracture healing throughout its entire life cycle, and simultaneously repairing bone and soft tissue. It is suitable for promoting the repair of various fractures and possesses extremely high clinical value and promising prospects for widespread application.

The invention discloses an injection ossein gel capable of tolerating irradiation sterilization and a preparation method of the injection ossein gel. The novel injectable bone grafting composite material is constructed by combining a bone targeting factor TBMP < 2 >-calcined bone scaffold with an injectable polyphosphazene-collagen-glycerin gel system. The invention aims to overcome the multiple bottlenecks of insufficient osteogenic ability, non-ideal repair effect, large operative wound and the like in the current bone defect treatment, and provides an innovative treatment strategy for clinical repair of large-volume maxillofacial bone defects. After the TBMP2 (at) TBC-polyphosphazene-collagen (at) glycerol injectable gel is subjected to irradiation sterilization, the gel form is not influenced, and the proliferation and osteogenic differentiation of the BMSCs can be obviously promoted.

The application provides bone reconstruction regulating polypeptides and application. On the basis of the first experiment of the inventors, bone reconstruction regulating polypeptides are designed and newly synthesized by using solid-phase polypeptide synthesis method through sequence alignment, structure analysis, physicochemical property and function prediction. The polypeptides have the characteristics of short length, simple synthesis, low cost, low cytotoxicity, high biological stability, moderate half-life, good bone targeting, and strong bone resorption regulating ability. The differentiation and function of osteoclasts and osteoblasts can be controlled by adjusting the concentration of the synthesized bone reconstruction regulating polypeptides, the ordered regulation of bone resorption and bone formation is realized, and the polypeptides have a broad potential application prospect.

The application discloses a CD301b + EXO and a preparation method thereof, and a bone-targeting microsphere loaded with the CD301b + EXO and an application thereof. The bone-targeting microsphere loaded with the CD301b + EXO is modified by a functional component of alendronate, is endowed with excellent bone targeting capability, can significantly improve the enrichment and retention of the exosome in a bone defect area, and realizes sustained and controllable release in a bone defect environment. The application fully plays the triple repair function of the CD301b + EXO, and realizes the synergistic promotion of the whole bone healing process by combining the bone targeting and slow-release characteristics of the microsphere, significantly improves the repair efficiency and quality, and has high clinical transformation value.

The present application relates to a kind of degradable cationic polymer with bone targeting function and its preparation method and application, specifically, the degradable cationic polymer with bone targeting function provided in the present application can effectively load nucleic acid (such as mRNA, miRNA, DNA), and the encapsulation efficiency is more than 90%, and stable nanoparticles are formed by electrostatic interaction, protect nucleic acid from degradation, while having the characteristics of slowing down particle positive electricity.This carrier shows better stability compared with LNP, supports long-term storage at low temperature.Its bone targeting is clear, promotes the accurate delivery of nucleic acid to bone tissue, enhances the efficacy and reduces side effects, while showing biocompatibility for osteoblasts without toxicity.The application range is wide, covering osteoporosis, osteoarthritis, bone tumor treatment and gene and stem cell therapy.Experiments have verified that the nanoparticles formed by the carrier and eGFP mRNA have high transfection efficiency, and the combination with miRNA effectively inhibits breast cancer bone metastasis, highlighting its therapeutic potential.

The invention provides a PLGA (poly (lactic-co-glycolic acid)-PEG (polyethylene glycol)-phosphonate polymer, a bone targeting nanoparticle as well as a preparation method and application of the bone targeting nanoparticle. According to the preparation method of the PLGA-PEG-phosphonate polymer, no catalyst is needed to participate in the reaction, the reaction can be carried out at room temperature, and the preparation method is a green chemical reaction method and is more suitable for large-scale process production requirements; the bone targeting nanoparticle is formed by self-assembly of the PLGA-PEG-phosphonate polymer and PLGA-PEG, the nanoparticle can be specifically combined with hydroxyapatite in bone tissue to realize targeting of the bone tissue, and the nanoparticle can be used as a drug carrier to deliver drugs to the bone tissue, so that the bone targeting nanoparticle has a good application prospect. And bone tissue related diseases such as osteoporosis, bone cancer and osteoarthritis can be treated.

The application discloses an acid oligopeptide modified bone targeting liposome and a preparation method thereof. The surface of the bone targeting liposome is modified with acid oligopeptide, and the modification density n is 1-5, which is expressed in mol%. The bone targeting liposome is prepared by modifying the liposome surface with D-glutamic acid hexapeptide which has bone targeting effect, and optimizing the modification density of the D-glutamic acid hexapeptide on the liposome surface, so that the liposome preparation with the strongest bone targeting activity is screened, and the bone tissue distribution of the liposome preparation is increased. With the increase of the modification density of the acid oligopeptide, the combination ability of the liposome and hydroxyapatite is enhanced. However, the high negative charge generated by the modification stimulates the phagocytosis of the mononuclear phagocyte system, and the blood retention of the liposome is reduced. However, the high-density acid oligopeptide modification (5 mol%) endows the liposome with outstanding HA combination activity, and the deficiency of the weak blood retention of the liposome is completely compensated, so that the highest bone targeting delivery is realized.

This invention discloses the application of sodium copper chlorophyllin in the preparation of drugs for treating osteoarthritis, belonging to the field of biomedical technology. The sodium copper chlorophyllin of this invention can block excessive activation of osteoclasts and subsequent angiogenesis, and successfully reprograms macrophage polarization, exhibiting significant anti-inflammatory and repairing effects. Furthermore, this invention constructs a dual-targeted sustained-release drug delivery system. After encapsulating sodium copper chlorophyllin into MOF material, the MOF material is then modified with MMP9 responsive peptides and bone-targeting peptides via click chemistry, achieving the goal of continuously releasing sodium copper chlorophyllin to the subchondral bone region of osteoarthritis.

The invention provides a bone targeting nucleic acid drug delivery system and a preparation method and application thereof, and is characterized in that active components of the bone targeting nucleic acid drug delivery system comprise strong electropositive lactoferrin nanoparticles for loading nucleic acid, bone targeting molecules and a nucleic acid drug, according to the nucleic acid-loaded strong electropositive lactoferrin nanoparticle, the surface of a lactoferrin nanoparticle is covalently modified with a polymerizable acryloyl group, the bone targeting molecule is alendronate, and the surface of the alendronate is covalently modified with a polymerizable acryloyl group. The nucleic acid medicine comprises one or more of small interfering ribonucleic acid, micro ribonucleic acid and nucleic acid aptamers with a bone resorption inhibiting function. The bone targeting nucleic acid drug delivery system is prepared by an in-situ free radical polymerization method. The prepared bone targeted delivery system can be efficiently delivered to bone tissue in a targeted mode, the function of osteoclasts can be regulated and controlled, the osteoinflammation microenvironment is improved by regulating immune balance, bone regeneration of osteoporosis diseases is effectively promoted, and the bone targeted delivery system can be widely applied to treatment of orthopedic diseases.

Provided herein are compositions and methods for targeted regulation of bone resorption. In particular, provided herein are compositions comprising a bone-anchored, activatable agent that provides activated local delivery of therapeutics to mineralized surfaces for localization of therapeutic effects.

This invention belongs to the field of biomedical technology and discloses a bone-targeting platform based on black phosphorus and amorphous calcium carbonate, its preparation method, and its applications. In this invention, amorphous calcium carbonate (ACC) is coated onto the surface of bone apatite (BP) to form the core (BA) of a nano-therapeutic platform. Subsequently, a polydopamine (PDA) layer is coated on the surface, and a bone-targeting ligand (polyacrylic acid-aldehyde PA) is further coupled to the PDA layer. After BA@PDA-PA precisely accumulates in bone tissue, it reacts to the acidic microenvironment and undergoes "autolysis-like" degradation, rapidly releasing BP and calcium carbonate. 2+ Released BP can scavenge ROS in the bone microenvironment and improve the bone immune microenvironment. Furthermore, BP and Ca... 2+ As a "raw material" for bone matrix mineralization, it synergistically promotes osteoogenesis and inhibits osteoclast activity, ultimately achieving a precise and multi-effect treatment for osteoporosis (OP).