14 results about "Bone Infection" patented technology

The invention discloses a bone infection and drug resistance prediction method and system fusing a knowledge graph and a graph convolutional network. The method comprises the following steps: preprocessing bone infection multi-source heterogeneous data to obtain a standardized data feature matrix; constructing a bone infection knowledge graph based on the matrix and obtaining a knowledge embedding matrix, and fusing the two to generate a medical semantic constraint fusion feature matrix; key medical variables are screened, the maximum information coefficient (MIC) of the key medical variables is calculated, and an adjacent matrix is constructed in combination with medical association strength factors; inputting the fusion feature matrix and the adjacent matrix into a GCN spatial feature extraction module and a BiGRU time sequence dependence capture module to obtain spatial and time sequence features, and fusing the spatial and time sequence features into a space-time fusion feature matrix; and inputting the result into a double-task prediction module, and outputting a bone infection and drug resistance grading probability prediction value. According to the invention, accurate and rapid prediction of bone infection prediction and drug resistance grading can be realized.

This invention discloses a method for preparing and applying a shell-core composite flexible biomaterial. The flexible biomaterial consists of an outer PMMA electrospun layer, a middle PTFF or PE layer, and a core PEG hydrogel; both the PMMA electrospun layer and the middle layer are porous. This flexible material is soft and elastic, exhibiting good film-forming and osteogenic induction effects. Animal experiments have confirmed that, compared to traditional bone cement, the flexible composite bone cement-induced biofilm shows an increase in CD31-positive cells (representing angiogenesis) and M2 macrophages (representing tissue repair), exhibiting better film-forming properties. It is a promising composite biomaterial that can be widely applied in the treatment of soft tissue defects, bone defects, and bone infections, achieving better therapeutic effects.

The application relates to the technical field of magnetic resonance image analysis, in particular to a DWI-ADC value dynamic atlas construction method and system for bone infection, which comprises the following steps: acquiring DWI and ADC data to establish an image index, screening an anatomical reference point to construct a lesion spatial relationship set, extracting a diffusion value to construct an ADC time sequence set, analyzing a trend to generate a segmented time label, dividing data to generate a node according to the label, and establishing a bone infection DWI-ADC dynamic atlas structure. In the application, parameter deviation is eliminated by image checking consistency and index structure establishment, a stable reference point of bone anatomy is used as a spatial calibration anchor point to overcome soft tissue edema interference, a lesion relative spatial relationship set is established to solidify coordinates, a lesion position is derived to ensure the anatomical identity of longitudinal comparison sampling parts, a lesion pathological turning signal is captured by arranging an ADC change trend label set, discrete data is converted into a continuous pathological model by constructing a dynamic atlas node sequence, and the bone infection evaluation accuracy is improved.

This invention belongs to the field of biomedical materials technology and discloses a highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability. The nanoparticle comprises zinc oxide nanoparticles with active sites and a bone-targeting material grafted onto the surface of the zinc oxide nanoparticles via the active sites. The active site is an amino group; the bone-targeting material is one of aspartic oligopeptide and glutamic acid oligopeptide. This invention also discloses the preparation method and application of the aforementioned highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability. The highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability provided by this invention can be directed to the site of bone infection through the targeting ability of the bone-targeting material for hydroxyapatite in bone tissue, and then exert the broad-spectrum antibacterial ability of the zinc oxide nanoparticles to kill bacteria.

The invention belongs to the technical field of medical materials, and particularly relates to a phosphatidylserine modified EGCG liposome, a preparation method and application of the phosphatidylserine modified EGCG liposome in bone infection resistance. The epigallocatechin gallate is entrapped in the liposome, a lipid bilayer of the epigallocatechin gallate comprises phosphatidylserine, phosphatidylcholine and cholesterol, and the molar ratio of the phosphatidylserine to the phosphatidylcholine to the cholesterol is controlled to be (1-2): 2: 1. The PS-EGCG liposome is prepared based on a film hydration method, the process is simple and convenient, and the obtained liposome is uniform in particle size, good in dispersity and stable in Zeta potential, can be effectively ingested by macrophages, plays the functions of inhibiting bacterial proliferation and regulating immune response, and is expected to be applied to treatment of infectious bone defects.

The invention relates to the technical field of medical detection, in particular to a wireless lactic acid sensor and bone infection monitoring system based on direct electron transferase, and the system comprises an enzymatic activity dynamic control module, a fluid shear lactic acid screening module, a node lactic acid abnormity association module, a bone infection monitoring module and an infection space mapping module. According to the invention, by monitoring and analyzing the correlation between an initial current increasing signal and the local temperature change of the micro-area, the enzyme activity can be effectively controlled and optimized, the detection accuracy is increased, lactic acid molecules are screened by adopting a fluid shear force gradient field, the lactic acid monitoring efficiency and resolution are further enhanced, and by designing the local micro-fluidic area, the detection accuracy is improved. The distribution of lactic acid molecules on the surface of the sensor can be more accurately controlled and predicted, more precise biochemical reaction monitoring is realized, the change trend of lactic acid concentration is combined with a bone tissue space model, a scientific basis is provided for accurately positioning a bone infection area, and the accuracy and effectiveness of early diagnosis are remarkably improved.

The invention relates to the technical field of artificial intelligence and machine learning, discloses a method for establishing an identification model of bone tuberculosis (STB) and suppurative bone infection (PSI) based on deep learning, and provides a novel interpretable deep learning model based on a Transform architecture, and the STB and the PSI are distinguished by using a diagnostic tag confirmed by metagenome next-generation sequencing. The TabPFN model provided by the invention not only shows strong diagnosis accuracy, but also has interpretability of early-stage STB recognition by utilizing the diagnosis tag confirmed by mNGS and common serological biomarkers, and provides instant early-stage diagnosis decision support for clinicians. According to the method, the TabPFN model is applied to diagnosis of the spinal bone tuberculosis for the first time, an efficient and accurate tool is provided, and a solid foundation is laid for future artificial intelligence auxiliary clinical diagnosis research.

The aim is to provide plasma biomarkers for bone infections in humans. [Solution] A plasma biomarker for bone infection comprising one or more substances selected from the group consisting of sphingosine-1-phosphate, taurocholic acid, glycololic acid, 11a,b hydroxyprogesterone, 15(S)-HETE-2, 13-dehydrochenodeoxycholic acid, 3,21-didihydroxy-5a-pregnane-20-one-2, aldosterone, bisacron-2, naringenin, tauro-α-mulicolic acid-6, 1-deoxysphingosine, 12(S)-HETE-2, 2-arachidonoylglycerol, 3-hydroxydodecanoic acid, 3-hydroxytetradecanoic acid, 3b-hydroxy-5-cholestic acid, AEA(22:4)-1, AEA(22:4)-2, bilbertin, sphingosine, malic acid, and succinic acid.

The application discloses a programmable drug release anti-infection and bone formation promoting multifunctional orthopedic internal implant device and a preparation method thereof. The implant is prepared according to the following steps: 1) introducing a titanium nanotube (TNT) structure on the surface of a titanium alloy base by using an anodic oxidation method; 2) sequentially loading an anti-infection component, a bone formation promoting factor and a blood vessel formation promoting factor into the TNT tube by using a vacuum sequential loading process; and 3) finally, coating a poly-tannic acid (PTA) coating on the surface of the material base by using a tannic acid (TA) in-situ self-polymerization technology. The multifunctional bone implant device can sequentially release the anti-infection drug, the blood vessel formation promoting factor and the bone formation promoting factor in the order of the vacuum drug loading, and the modified surface of the device has good drug release and oxidation resistance, so that the bone infection can be efficiently prevented and treated, and the bone repair efficiency is improved.

The application discloses a kind of based on space-time sequential nature magnetic control membrane rupture and release sterilization mechanism multiple antibacterial composite coating material and its preparation method and application, preparation method is as follows: 1) using micro-arc oxygen technology is constructed microporous structure on the surface of titanium-based instrument after pretreatment;2) using emulsion self-assembly method constructs quaternary ammonium chitosan and negative charged antibacterial agent nanoparticle, using one pulse electrophoresis process is loaded in micropore nanoparticle;3) Fe3O4 NPs are prepared by hydrothermal method, and Fe3O4 NPs are controlled in micropore by secondary pulse electrophoresis process, after washing, drying and irradiation sterilization, antibacterial composite coating is obtained;The material can be driven Fe3O4 NPs on titanium-based instrument to generate torque to destroy bacterial biofilm by applying reverse circular polarized magnetic field, and promote the inner layer to release antibacterial agent, realize space-time sequential nature magnetic control membrane rupture and release sterilization, realize efficient treatment for bone infection.

The invention discloses a bone infection composite gel coating pre-solution based on an in-situ injection-in-vivo curing technology as well as a preparation method and an application system of the bone infection composite gel coating pre-solution. The preparation method specifically comprises the following steps: 1) preparing a composite gel coating pre-solution by using methylacryloylated hyaluronic acid, catechol group and nitrobenzene group modified hyaluronic acid, EPS hydrolase, active antibacterial agent loaded methylacryloylated hyaluronic acid microspheres and a photoinitiator; 2) forming an application system by the pre-solution, an endoscopic active material conveying conduit, a conduit controller assembly, a mechanical arm assembly and an imaging assembly; and (3) the catheter controller assembly and the mechanical arm assembly control the endoscopic active material conveying catheter to enter the body through the minimally invasive wound, the imaging assembly performs observation and adjustment until the head of the catheter reaches the surface of a bone implantation instrument, the pre-solution is conveyed to the surface of the instrument, and a UV light source is switched for curing to form an antibacterial gel coating. Starting from the time point after in-vivo infection occurs, symptomatic treatment can be carried out on the infection after the orthopedic instrument implantation operation.

The invention relates to lactococcus lactis microvesicles capable of treating chronic bone infection and application of the lactococcus lactis microvesicles, and a nano vaccine capable of treating chronic bone infection. The nano vaccine provided by the invention can execute a biphase sequential immunotherapy effect: (i) early DC activation establishes specific antibacterial humoral immunity to prevent primary and recurrent MRSA challenges; (ii) the inflammatory macrophages at the defect part are reprogrammed through later-stage targeted intracellular NAD + delivery, inflammation is eliminated, establishment of a bone regeneration microenvironment is promoted, and effective long-term bone repair is achieved. The nano vaccine treatment strategy provides a promising strategy for continuous integrated antibacterial immunotherapy and regenerative therapy for intractable MRSA-related chronic bone infection.

The application belongs to the technical field of medical apparatus and discloses an ultrasonic antibacterial device for treating bone infection, which comprises a main control shell and a minimally invasive catheter. The main control shell is internally provided with a guide wire control module, an ultrasonic control module and a medium storage cavity. The minimally invasive catheter is of a double-layer structure and is internally provided with a guide wire. The front end of the minimally invasive catheter is provided with an annular ultrasonic medium cavity, which is used for filling ultrasonic medium solution. The ultrasonic medium cavity is internally provided with an ultrasonic generating assembly arranged in an annular array. The ultrasonic generating assembly is internally provided with a piezoelectric wafer, which is used for realizing electric-acoustic conversion to realize emission and acceptance of ultrasonic waves. When the device is used, the infection site is accurately positioned through the guide wire, ultrasonic waves are emitted through the ultrasonic generating assembly, and the structure of bacterial biofilm is destroyed through ultrasonic cavitation effect, so that the bacterial biofilm is removed and the antibacterial treatment effect is achieved. The device is suitable for bone infection treatment.

The invention belongs to the technical field of biological medicine, provides a method for constructing a biosafety mouse bone tuberculosis model based on mycobacterium smegmatis, and evaluates that under the condition of a standard biosafety level (BSL)-1, the mycobacterium smegmatis induces local and disseminated bone infection through three infection pathways, and the bone tuberculosis model has the advantages that the bone tuberculosis is induced by the mycobacterium smegmatis through three infection pathways; results show that the mouse model for intracentral injection administration of the three infection pathways is closer to the latent progress of human bone tuberculosis. The mouse model based on the mycobacterium smegmatis provides a simple and reliable platform related to immunopathology for exploring host-pathogen dynamics, immunologically-driven bone destruction and early treatment tests.