23 results about "Bone Infection" patented technology

The invention discloses a bone infection and drug resistance prediction method and system fusing a knowledge graph and a graph convolutional network. The method comprises the following steps: preprocessing bone infection multi-source heterogeneous data to obtain a standardized data feature matrix; constructing a bone infection knowledge graph based on the matrix and obtaining a knowledge embedding matrix, and fusing the two to generate a medical semantic constraint fusion feature matrix; key medical variables are screened, the maximum information coefficient (MIC) of the key medical variables is calculated, and an adjacent matrix is constructed in combination with medical association strength factors; inputting the fusion feature matrix and the adjacent matrix into a GCN spatial feature extraction module and a BiGRU time sequence dependence capture module to obtain spatial and time sequence features, and fusing the spatial and time sequence features into a space-time fusion feature matrix; and inputting the result into a double-task prediction module, and outputting a bone infection and drug resistance grading probability prediction value. According to the invention, accurate and rapid prediction of bone infection prediction and drug resistance grading can be realized.

The invention discloses nano-particles with antibacterial and osteogenesis promoting effects as well as a preparation method and application of the nano-particles, and belongs to the technical field of antibacterial nano-materials. On one hand, the invention provides the nano-particles with antibacterial and osteogenesis promoting effects and the preparation method thereof, and on the other hand, the invention provides the application of the nano-particles. The nanoparticles obtained by the method disclosed by the invention have good stability under physiological conditions (PH = 7.4); polyphenol, metal ions and AMP (adenosine monophosphate) can be slowly released in an inflammatory (weakly acidic) environment, and the functions including but not limited to bacterium capturing, bacterium killing, inflammation and oxidation resisting, osteogenesis promoting and the like are realized; the nano-particles can be used for preparing a bone infection treatment drug, and the bone infection treatment drug has a broad-spectrum antibacterial effect and cannot cause antibiotic drug resistance; the treatment medicine can efficiently prevent and treat bone infection and improve bone repair efficiency.

This invention discloses a method for preparing and applying a shell-core composite flexible biomaterial. The flexible biomaterial consists of an outer PMMA electrospun layer, a middle PTFF or PE layer, and a core PEG hydrogel; both the PMMA electrospun layer and the middle layer are porous. This flexible material is soft and elastic, exhibiting good film-forming and osteogenic induction effects. Animal experiments have confirmed that, compared to traditional bone cement, the flexible composite bone cement-induced biofilm shows an increase in CD31-positive cells (representing angiogenesis) and M2 macrophages (representing tissue repair), exhibiting better film-forming properties. It is a promising composite biomaterial that can be widely applied in the treatment of soft tissue defects, bone defects, and bone infections, achieving better therapeutic effects.

The invention relates to the technical field of biological materials, in particular to a multi-shell drug-loaded microsphere, a preparation method thereof and application of the multi-shell drug-loaded microsphere in bone infection treatment. A multi-shell drug-loaded microsphere comprises: a core body formed by calcium phosphate loaded with an osteogenic drug; the degradable shell wraps the core body, and the shell contains antibiotics; the surface of the shell is coated with the metal-polyphenol complex network. According to the technical scheme provided by the invention, the osteogenesis medicine is loaded in the core body, and the antibiotics are integrated in the shell, so that partitioned loading of the medicine is realized. And the shell is made of a degradable material, so that the osteogenic drug and the antibiotics can be slowly released along with the degradation process of the osteogenic drug and the antibiotics. In the structural design, the shell forms a physical barrier for the core body, and the release rate of the osteogenesis medicine is effectively limited, so that the overall release time of the two medicines is synergistically prolonged.

The application relates to the technical field of magnetic resonance image analysis, in particular to a DWI-ADC value dynamic atlas construction method and system for bone infection, which comprises the following steps: acquiring DWI and ADC data to establish an image index, screening an anatomical reference point to construct a lesion spatial relationship set, extracting a diffusion value to construct an ADC time sequence set, analyzing a trend to generate a segmented time label, dividing data to generate a node according to the label, and establishing a bone infection DWI-ADC dynamic atlas structure. In the application, parameter deviation is eliminated by image checking consistency and index structure establishment, a stable reference point of bone anatomy is used as a spatial calibration anchor point to overcome soft tissue edema interference, a lesion relative spatial relationship set is established to solidify coordinates, a lesion position is derived to ensure the anatomical identity of longitudinal comparison sampling parts, a lesion pathological turning signal is captured by arranging an ADC change trend label set, discrete data is converted into a continuous pathological model by constructing a dynamic atlas node sequence, and the bone infection evaluation accuracy is improved.

The present application provides a composite sonosensitizer and its bacterial target delivery system BPT-ICG@Bd capable of treating pseudomonas aeruginosa biofilm related infection, wherein the composite sonosensitizer (BPT-ICG) is composed of inorganic sonosensitizer (BPT) and organic sonosensitizer (ICG), Bd is natural gram-negative bacteria predator Bdellovibrio, the composite sonosensitizer (BPT-ICG) is modified to the surface of Bdellovibrio (Bd) by polydopamine to form BPT-ICG@Bd. The high-speed movement characteristics of Bdellovibrio predator gram-negative bacteria are used to destroy the biofilm structure, invade bacteria, and at the same time, the composite sonosensitizer is triggered to produce a large amount of active oxygen by applying ultrasound, so as to quickly remove the biofilm and bacteria, and be used for biofilm related lung infection and bone infection.

This invention belongs to the field of biomedical materials technology and discloses a highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability. The nanoparticle comprises zinc oxide nanoparticles with active sites and a bone-targeting material grafted onto the surface of the zinc oxide nanoparticles via the active sites. The active site is an amino group; the bone-targeting material is one of aspartic oligopeptide and glutamic acid oligopeptide. This invention also discloses the preparation method and application of the aforementioned highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability. The highly efficient antibacterial zinc oxide nanoparticle with bone targeting capability provided by this invention can be directed to the site of bone infection through the targeting ability of the bone-targeting material for hydroxyapatite in bone tissue, and then exert the broad-spectrum antibacterial ability of the zinc oxide nanoparticles to kill bacteria.

The invention belongs to the technical field of medical materials, and particularly relates to a phosphatidylserine modified EGCG liposome, a preparation method and application of the phosphatidylserine modified EGCG liposome in bone infection resistance. The epigallocatechin gallate is entrapped in the liposome, a lipid bilayer of the epigallocatechin gallate comprises phosphatidylserine, phosphatidylcholine and cholesterol, and the molar ratio of the phosphatidylserine to the phosphatidylcholine to the cholesterol is controlled to be (1-2): 2: 1. The PS-EGCG liposome is prepared based on a film hydration method, the process is simple and convenient, and the obtained liposome is uniform in particle size, good in dispersity and stable in Zeta potential, can be effectively ingested by macrophages, plays the functions of inhibiting bacterial proliferation and regulating immune response, and is expected to be applied to treatment of infectious bone defects.

The purpose is to provide plasma biomarkers for bone infections in humans. [Solution] The plasma biomarker for bone infection consists of one or more substances selected from the group consisting of sphingosine-1-phosphate, taurocholic acid, glycololic acid, 11a,b-hydroxyprogesterone, 15(S)-HETE-2, 13-dehydrochenodeoxycholic acid, 3,21-dihydroxy-5a-pregnan-20-one-2, aldosterone, bisacrone-2, naringenin, tauro-a-muricholic acid-6, 1-deoxysphingosine, 12(S)-HETE-2, 2-arachidonoylglycerol, 3-hydroxydodecanoic acid, 3-hydroxytetradecanoic acid, 3b-hydroxy-5-cholestenoic acid, AEA(22:4)-1, AEA(22:4)-2, bilverdin, sphingosine, malic acid, and succinic acid.

The invention relates to the technical field of needle biopsy, in particular to a needle biopsy device for bone infection, which comprises a puncture cannula, the puncture cannula is provided with a puncture suite, the puncture suite comprises a puncture guide wire, one end of the puncture guide wire is connected with a puncture needle, and the puncture needle sequentially comprises a needle tip section, a needle body middle section and a needle tail section. The needle tip section is provided with a diamond-like film coating, and the surface of the needle body middle section is provided with a spiral groove; the puncture cannula is further provided with a biopsy sampling suite, the biopsy sampling suite comprises biopsy forceps, a sleeve is fixedly connected to the outer side of the biopsy forceps, and a barrel saw assembly used for rotationally cutting and collecting bone tissue samples is connected to the sleeve in a sliding mode. The strength of the needle point of the puncture needle is strengthened through design, gradient rigidity of the needle body is achieved, integrated sampling of bone tissue and soft tissue is integrated, and the problems of traditional fine needle puncture angle deviation, insufficient sampling amount and channel deformation are solved.

The invention relates to the technical field of medical detection, in particular to a wireless lactic acid sensor and bone infection monitoring system based on direct electron transferase, and the system comprises an enzymatic activity dynamic control module, a fluid shear lactic acid screening module, a node lactic acid abnormity association module, a bone infection monitoring module and an infection space mapping module. According to the invention, by monitoring and analyzing the correlation between an initial current increasing signal and the local temperature change of the micro-area, the enzyme activity can be effectively controlled and optimized, the detection accuracy is increased, lactic acid molecules are screened by adopting a fluid shear force gradient field, the lactic acid monitoring efficiency and resolution are further enhanced, and by designing the local micro-fluidic area, the detection accuracy is improved. The distribution of lactic acid molecules on the surface of the sensor can be more accurately controlled and predicted, more precise biochemical reaction monitoring is realized, the change trend of lactic acid concentration is combined with a bone tissue space model, a scientific basis is provided for accurately positioning a bone infection area, and the accuracy and effectiveness of early diagnosis are remarkably improved.

The invention relates to the technical field of artificial intelligence and machine learning, discloses a method for establishing an identification model of bone tuberculosis (STB) and suppurative bone infection (PSI) based on deep learning, and provides a novel interpretable deep learning model based on a Transform architecture, and the STB and the PSI are distinguished by using a diagnostic tag confirmed by metagenome next-generation sequencing. The TabPFN model provided by the invention not only shows strong diagnosis accuracy, but also has interpretability of early-stage STB recognition by utilizing the diagnosis tag confirmed by mNGS and common serological biomarkers, and provides instant early-stage diagnosis decision support for clinicians. According to the method, the TabPFN model is applied to diagnosis of the spinal bone tuberculosis for the first time, an efficient and accurate tool is provided, and a solid foundation is laid for future artificial intelligence auxiliary clinical diagnosis research.

The invention belongs to the technical field of medical instruments, and discloses an ultrasonic antibacterial device for treating bone infection, which comprises a main control shell and a minimally invasive catheter. A guide wire control module, an ultrasonic control module and a medium liquid storage cavity are arranged in the main control shell, the minimally invasive catheter is of a double-layer structure, and a guide wire is arranged in the minimally invasive catheter. An annular ultrasonic medium cavity is formed in the front end of the minimally invasive catheter, the ultrasonic medium cavity is used for being filled with an ultrasonic medium solution, ultrasonic generation assemblies are arranged in the minimally invasive catheter in an annular array mode, piezoelectric wafers are arranged in the ultrasonic generation assemblies, and the piezoelectric wafers are used for achieving electricity-sound conversion to achieve emission and receiving of ultrasonic waves. When the device is used, an infected part is accurately positioned through the guide wire, ultrasonic waves are emitted by utilizing the ultrasonic generation assembly, and a bacterial biofilm structure is destroyed by utilizing an ultrasonic cavitation effect, so that the effects of removing the bacterial biofilm and performing antibacterial treatment are achieved. The device is suitable for bone infection treatment.

The aim is to provide plasma biomarkers for bone infections in humans. [Solution] A plasma biomarker for bone infection comprising one or more substances selected from the group consisting of sphingosine-1-phosphate, taurocholic acid, glycololic acid, 11a,b hydroxyprogesterone, 15(S)-HETE-2, 13-dehydrochenodeoxycholic acid, 3,21-didihydroxy-5a-pregnane-20-one-2, aldosterone, bisacron-2, naringenin, tauro-α-mulicolic acid-6, 1-deoxysphingosine, 12(S)-HETE-2, 2-arachidonoylglycerol, 3-hydroxydodecanoic acid, 3-hydroxytetradecanoic acid, 3b-hydroxy-5-cholestic acid, AEA(22:4)-1, AEA(22:4)-2, bilbertin, sphingosine, malic acid, and succinic acid.

The application discloses a programmable drug release anti-infection and bone formation promoting multifunctional orthopedic internal implant device and a preparation method thereof. The implant is prepared according to the following steps: 1) introducing a titanium nanotube (TNT) structure on the surface of a titanium alloy base by using an anodic oxidation method; 2) sequentially loading an anti-infection component, a bone formation promoting factor and a blood vessel formation promoting factor into the TNT tube by using a vacuum sequential loading process; and 3) finally, coating a poly-tannic acid (PTA) coating on the surface of the material base by using a tannic acid (TA) in-situ self-polymerization technology. The multifunctional bone implant device can sequentially release the anti-infection drug, the blood vessel formation promoting factor and the bone formation promoting factor in the order of the vacuum drug loading, and the modified surface of the device has good drug release and oxidation resistance, so that the bone infection can be efficiently prevented and treated, and the bone repair efficiency is improved.

The invention discloses an ultrasonic-driven PLGA / Zn-KNN piezoelectric antibacterial scaffold material as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The PLGA / Zn-KNN piezoelectric antibacterial scaffold material provided by the invention is prepared by combining zinc oxide (ZnO) into potassium sodium niobate (KNN), and is compounded with polylactic acid-glycolic acid copolymer (PLGA) to realize multi-combination antibiosis for bone infection. Moreover, the physical and chemical properties of the PLGA / Zn-KNN piezoelectric antibacterial scaffold material are analyzed, and bacteria, cell and animal experiments are carried out to characterize the antibacterial and infection treatment capabilities of the piezoelectric scaffold. According to the invention, ZnO particles are embedded into a KNN solid solution matrix, so that the piezoelectric property of the PLGA / Zn-KNN stent is enhanced. In addition, the PLGA / Zn-KNN piezoelectric antibacterial scaffold material prepared by the invention releases zinc ions, and ultrasonic-driven electrical stimulation generates a remarkable antibacterial effect through direct and immunoregulation antibacterial ways.

The application discloses a kind of bone infection image acquisition method and system based on bacterial autofluorescence.The bone infection image acquisition method based on bacterial autofluorescence includes: obtaining the binary mask and three-dimensional topographic model of the tissue to be measured;According to the three-dimensional topographic model of the tissue to be measured, illumination correction chart and collection correction chart are generated;Control light source module to the tissue to be measured Time emission first wavelength fluorescence, utilize light field modulation module based on illumination correction chart to modulate illumination light field, time acquisition first wavelength and second wavelength fluorescence corresponding fluorescence image, and generate background fluorescence image (for first wavelength) and total fluorescence image (for second wavelength);According to total fluorescence image and background fluorescence image, bacterial fluorescence image is generated.The application eliminates the light intensity error caused by distance and angle through three-dimensional topographic correction, and significantly reduces the interference of tissue background fluorescence through double-wavelength difference strategy, improves the imaging quality.

The application discloses a kind of based on space-time sequential nature magnetic control membrane rupture and release sterilization mechanism multiple antibacterial composite coating material and its preparation method and application, preparation method is as follows: 1) using micro-arc oxygen technology is constructed microporous structure on the surface of titanium-based instrument after pretreatment;2) using emulsion self-assembly method constructs quaternary ammonium chitosan and negative charged antibacterial agent nanoparticle, using one pulse electrophoresis process is loaded in micropore nanoparticle;3) Fe3O4 NPs are prepared by hydrothermal method, and Fe3O4 NPs are controlled in micropore by secondary pulse electrophoresis process, after washing, drying and irradiation sterilization, antibacterial composite coating is obtained;The material can be driven Fe3O4 NPs on titanium-based instrument to generate torque to destroy bacterial biofilm by applying reverse circular polarized magnetic field, and promote the inner layer to release antibacterial agent, realize space-time sequential nature magnetic control membrane rupture and release sterilization, realize efficient treatment for bone infection.

The invention discloses a bone infection composite gel coating pre-solution based on an in-situ injection-in-vivo curing technology as well as a preparation method and an application system of the bone infection composite gel coating pre-solution. The preparation method specifically comprises the following steps: 1) preparing a composite gel coating pre-solution by using methylacryloylated hyaluronic acid, catechol group and nitrobenzene group modified hyaluronic acid, EPS hydrolase, active antibacterial agent loaded methylacryloylated hyaluronic acid microspheres and a photoinitiator; 2) forming an application system by the pre-solution, an endoscopic active material conveying conduit, a conduit controller assembly, a mechanical arm assembly and an imaging assembly; and (3) the catheter controller assembly and the mechanical arm assembly control the endoscopic active material conveying catheter to enter the body through the minimally invasive wound, the imaging assembly performs observation and adjustment until the head of the catheter reaches the surface of a bone implantation instrument, the pre-solution is conveyed to the surface of the instrument, and a UV light source is switched for curing to form an antibacterial gel coating. Starting from the time point after in-vivo infection occurs, symptomatic treatment can be carried out on the infection after the orthopedic instrument implantation operation.

The invention discloses a nano heterojunction material with ultrasonic response sterilization aiming at pain points of clinical bacterial infection. The nano heterojunction material can promote osteogenic differentiation of stem cells and vascularization of endothelial cells. The nano heterojunction is composed of CuFe2O4 nano particles and MoS2 quantum dots, and is prepared by a hydrothermal one-pot method. The nano heterojunction prepared by the method has good cell compatibility, and can respond to ultrasonic stimulation to generate hydroxyl radicals, singlet oxygen and other active oxygen components to kill surrounding bacteria. Besides, copper and iron ions released by the nano heterojunction can promote the expression of osteogenesis related genes of stem cells and promote the expression of angiogenesis related genes of endothelial cells, so that the effect of accelerating bone repair is achieved. The method can be widely applied to the fields related to bone infection treatment or other bacterial infection or bacterial pollution.

The invention relates to lactococcus lactis microvesicles capable of treating chronic bone infection and application of the lactococcus lactis microvesicles, and a nano vaccine capable of treating chronic bone infection. The nano vaccine provided by the invention can execute a biphase sequential immunotherapy effect: (i) early DC activation establishes specific antibacterial humoral immunity to prevent primary and recurrent MRSA challenges; (ii) the inflammatory macrophages at the defect part are reprogrammed through later-stage targeted intracellular NAD + delivery, inflammation is eliminated, establishment of a bone regeneration microenvironment is promoted, and effective long-term bone repair is achieved. The nano vaccine treatment strategy provides a promising strategy for continuous integrated antibacterial immunotherapy and regenerative therapy for intractable MRSA-related chronic bone infection.

The application belongs to the technical field of medical apparatus and discloses an ultrasonic antibacterial device for treating bone infection, which comprises a main control shell and a minimally invasive catheter. The main control shell is internally provided with a guide wire control module, an ultrasonic control module and a medium storage cavity. The minimally invasive catheter is of a double-layer structure and is internally provided with a guide wire. The front end of the minimally invasive catheter is provided with an annular ultrasonic medium cavity, which is used for filling ultrasonic medium solution. The ultrasonic medium cavity is internally provided with an ultrasonic generating assembly arranged in an annular array. The ultrasonic generating assembly is internally provided with a piezoelectric wafer, which is used for realizing electric-acoustic conversion to realize emission and acceptance of ultrasonic waves. When the device is used, the infection site is accurately positioned through the guide wire, ultrasonic waves are emitted through the ultrasonic generating assembly, and the structure of bacterial biofilm is destroyed through ultrasonic cavitation effect, so that the bacterial biofilm is removed and the antibacterial treatment effect is achieved. The device is suitable for bone infection treatment.

The invention belongs to the technical field of biological medicine, provides a method for constructing a biosafety mouse bone tuberculosis model based on mycobacterium smegmatis, and evaluates that under the condition of a standard biosafety level (BSL)-1, the mycobacterium smegmatis induces local and disseminated bone infection through three infection pathways, and the bone tuberculosis model has the advantages that the bone tuberculosis is induced by the mycobacterium smegmatis through three infection pathways; results show that the mouse model for intracentral injection administration of the three infection pathways is closer to the latent progress of human bone tuberculosis. The mouse model based on the mycobacterium smegmatis provides a simple and reliable platform related to immunopathology for exploring host-pathogen dynamics, immunologically-driven bone destruction and early treatment tests.