27 results about "Osteoporotic bone" patented technology

The invention relates to the technical field of biology, in particular to application of a GGPS1 activator, a pharmaceutical composition, a kit and a method. The invention relates to bone metabolism regulation and bone regeneration. It is found that improvement of GGPS1 expression / activity can activate TGF-beta / Smad2 / 3 by down-regulating LTBP1, so that human bone marrow mesenchymal stem cells are converted from adipogenesis to osteogenesis, and bone formation and bone defect repair are promoted. The invention provides a drug containing a GGPS1 activator and a controlled release composition for implantation, which are used for osteoporosis, bone mass reduction, delayed fracture healing and the like. The invention also provides an in-vitro screening and evaluation method and a detection kit, and the indexes such as p-Smad2 / 3, alizarin red, oil red O and the like are used for judgment. The invention realizes upstream heavy balance and has the advantages of bone promotion and fat suppression.

The invention discloses a bionic type tibia intramedullary nail and a matched instrument thereof, and relates to the technical field of medical instruments, and the bionic type tibia intramedullary nail comprises an intramedullary nail body; the intramedullary nail body comprises an intramedullary nail near end, an intramedullary nail middle section and an intramedullary nail far end, the intramedullary nail middle section is inserted into the intramedullary nail near end in a threaded mode, and the intramedullary nail far end is inserted into the intramedullary nail middle section in a threaded mode; according to the intramedullary nail, through the sectional type thread inserting design of the intramedullary nail near end, the intramedullary nail middle section and the intramedullary nail far end and the matching of the variable pitch gradient structures of the near end thread, the middle section thread and the far end thread, precise adaptation of the intramedullary nail and the anatomical form of the tibia marrow cavity is achieved, and a gradient mechanical buffering system is formed by the near end thread, the middle section thread and the far end thread; the problem of stress shielding caused by the fact that rigidity of a traditional intramedullary nail is not matched with a skeleton is effectively solved, meanwhile, the variable-pitch threads and the thread depth gradual change design are adopted, damage to an endosteum during nail placement is reduced, uniform stress conduction is achieved, local stress concentration is avoided, and the risks of osteoporosis, delayed fracture healing and secondary fracture are reduced.

The invention provides a shape memory nano short fiber stent with self-adaptive capacity and preparation and application thereof, and belongs to the technical field of tissue engineering materials. The preparation method comprises the following steps: firstly, preparing flexible polylactic acid-fullerol composite nanofibers containing uniformly dispersed fullerol nanocrystals by utilizing an electrospinning technology, then dividing the flexible polylactic acid-fullerol composite nanofibers into short fibers by virtue of homogenization and freeze-drying processes, and assembling the short fibers into a three-dimensional porous scaffold by taking chitosan as an adhesive. The porous scaffold material disclosed by the invention has excellent biological characteristics and a water-triggered shape memory effect, and due to the fact that chitosan has excellent antioxidant activity and inherent bone induction characteristics, the nanofiber scaffold with the controllable and recoverable shape developed by the invention is expected to effectively promote osteoporotic bone healing.

The invention discloses a bone targeting exosome polypeptide delivery system based on a biological orthogonal strategy and a preparation method and application thereof, and relates to the technical field of biological medicine and drug delivery. The bone targeting exosome polypeptide delivery system based on the biological orthogonal strategy comprises an engineered exosome as a carrier and an osteogenesis-promoting therapeutic polypeptide, wherein the osteogenesis-promoting therapeutic polypeptide is anchored on the membrane surface of the engineered exosome; a fusion protein is displayed on the membrane surface of the engineered exosome, and the fusion protein is formed by fusing a bone targeting ligand and an industrial exosome transmembrane scaffold protein; the osteogenesis-promoting therapeutic polypeptide is connected with the industrial exosome through the flexible spacer arm, and the membrane surface steric hindrance is reduced through the flexible spacer arm so as to improve the accessibility of the active fragment of the polypeptide; the delivery system provided by the invention can improve the targeted enrichment and treatment efficiency of osteogenesis promoting drugs in bone tissues, and is suitable for intervention of bone metabolism related diseases such as osteoporosis, delayed fracture healing, bone defects and the like.

The application provides a traditional Chinese medicine granule for improving osteoporosis and a preparation method and application thereof, and belongs to the technical field of medicines. Codonopsis pilosula, drynaria rhizome, epimedium and eucommia are subjected to deep eutectic solvent extraction, purification, and preparation of a traditional Chinese medicine extract, water extraction, dialysis and drying of pilose antler to prepare pilose antler protein peptide; water extraction and alcohol precipitation of cistanche to prepare cistanche polysaccharide; after modification of hydroxyapatite by citric acid, sodium dihydrogen phosphate and beta-cyclodextrin, the product is prepared by inclusion of calcium salt, alpha-cholecalciferol and vitamin D3, and then mixed with the traditional Chinese medicine extract, the pilose antler protein peptide and the cistanche polysaccharide to prepare the traditional Chinese medicine granule for improving osteoporosis. The prepared traditional Chinese medicine granule for improving osteoporosis can promote the synthesis of collagen and bone matrix protein, inhibit bone resorption, promote bone remodeling and enhance the activity of osteoblasts, and has a good improvement effect on osteoporosis, bone fracture and other symptoms, and has a wide application prospect.

The invention provides a risk prediction method and device for suffering from osteoporotic fracture. The method comprises the following steps: counting basic information of a target person; performing VBQ scoring based on the magnetic resonance imaging of the target person; calculating an HU value based on the CT image of the target person; and substituting the counted basic information of the target person and the calculated VBQ score and HU value into a score table to obtain the osteoporosis fracture risk grade of the target person. According to the invention, the risk of the osteoporotic fracture of the target person can be predicted more accurately, so that targeted prevention is carried out, and the harm of the osteoporotic fracture to middle-aged and elderly people is reduced.

The invention discloses hydrogel for osteoporotic bone defect repair and a preparation method of the hydrogel. The hydrogel comprises basic hydrogel and mineralized photosynthetic microorganisms, the mineralized photosynthetic microorganism comprises a photosynthetic microorganism, a mineralized shell layer arranged on the surface of the photosynthetic microorganism, and rare earth up-conversion nano-particles loaded in cells of the photosynthetic microorganism, and the rare earth up-conversion nano-particles can convert near-infrared light into visible light. According to the present invention, the oxygen production type mineralized microalgae composite hydrogel with characteristics of deep tissue activation, controllable oxygen production and simultaneous realization of four-effect synergy of acid neutralization, continuous oxygen supply, angiogenesis promotion and osteogenesis mineralization can be provided, such that osteoblast activity can be promoted, osteoclast activity can be inhibited, in-vivo osteogenesis can be promoted, and the technical support can be provided for osteoporotic bone defect repair.

The application provides a strontium-doped polypeptide hydrogel and a preparation method, a composite biological scaffold and an application, and belongs to the technical field of biomedical materials.The strontium-doped polypeptide hydrogel comprises a three-dimensional nanofiber network formed by self-assembly of polypeptide phosphonated derivatives, and the three-dimensional nanofiber network is loaded with strontium ions.The three-dimensional nanofiber network is formed by self-assembly of strontium ions and polypeptide phosphonated derivatives, a novel hydrogel with mechanical adaptability, controllable degradability and bidirectional regulation function of osteogenesis-osteoclast is obtained, local targeted delivery of strontium ions is realized, and the functional regeneration of osteoporotic bone defects is realized in cooperation with the bone adhesion properties of polypeptides.

The utility model discloses an absorbable osteoporotic fracture fixing device which comprises a fixing plate, suture anchor knots and suture lines, the fixing plate is made of absorbable materials, the fixing plate and the suture anchor knots are located on the two sides of a fracture part respectively, and the fixing plate is located on the side close to a wound; the suture line is arranged on the fixing plate and the suture anchor knot in a penetrating mode and used for connecting the fixing plate and the suture anchor knot, and the suture line is tensioned so as to drive the fixing plate and the suture anchor knot to conduct compression fixation on the fracture part. The absorbable fixing plate and the suture anchor knots are arranged on the two sides of the fracture position to pressurize and fix the fracture position, the fixing plate can be absorbed, the step of opening a wound to take out the fixing plate in the later period is avoided, and the operation is more convenient.

The application discloses a kind of prediction method and prediction device of osteoporosis patient fracture risk.The prediction method includes: the individualized factor data and bone density data of batch osteoporosis patients are carried out multiple regression analysis, and multiple regression equation is established;Establish the lumbar finite element model of each osteoporosis patient;Simulation analysis each lumbar finite element model is biomechanics change when being stressed under several daily scenes, obtain the proportion of the vertebral cancellous bone unit microstrain of each lumbar finite element model exceeding threshold value;According to the proportion of the vertebral cancellous bone unit microstrain exceeding threshold value, the bone density corresponding to each lumbar finite element model is carried out correlation analysis, and obtains osteoporosis fracture risk prediction equation;According to the individualized factor data of to-be-predicted object, multiple regression equation and osteoporosis fracture risk prediction equation, fracture risk assessment result is obtained.The method considers the influence of individualized factor on bone density, and it is favorable to accurately carry out fracture risk prediction.

The invention discloses an osteoporotic bone sample sampling device, and belongs to the technical field of sample sampling, the osteoporotic bone sample sampling device comprises a sampling platform, one side of the sampling platform is provided with a control panel, and the center of the surface of the top end of the sampling platform is welded with a material guide disc; meanwhile, the surface of the top end of the material guiding disc is communicated with a collecting box at the bottom of the sampling platform, adjustable fixed rotating assemblies are welded to the two sides of the material guiding disc and used for clamping and fixing bone tissue samples of different positions and different densities, and multi-direction rotating assemblies are welded to the tops of the adjustable fixed rotating assemblies and used for drilling the bone samples from multiple angles. By adopting the adjustable fixed rotating assembly and the multi-direction rotating assembly, the equipment can perform multi-angle and multi-direction sampling at the same position and in the same direction, the heterogeneity of bone tissues can be captured more comprehensively, and errors or local deviation possibly generated by single-direction sampling are avoided.

The invention relates to the technical field of bone metabolism disease related markers, in particular to application of CASQ1 protein in preparation of bone metabolism related disease diagnosis and / or screening products. It is found that when the muscle function is abnormal or damaged, the content of CASQ1 protein in exosomes secreted by muscle cells is increased, the exosomes can be transferred to adjacent bone tissue, the osteogenic differentiation of bone marrow mesenchymal stem cells is inhibited, and then bone metabolism disorder is caused. The invention provides a diagnostic kit and a pharmaceutical composition taking CASQ1 protein as a detection target. The diagnostic kit and the pharmaceutical composition are used for early screening and diagnosis of bone metabolic diseases such as myogenic osteoporosis, fracture healing disorder and bone dysplasia. By detecting the content change of the CASQ1 protein in tissues or body fluids, quantitative evaluation of muscle dysfunction related bone metabolism abnormality can be realized, and a new detection means and a biological target are provided for clinical diagnosis and treatment intervention of related diseases.

The application discloses a borosilicate bioactive glass and a preparation method and application thereof, and belongs to the technical field of biomedical materials. The bioactive glass comprises the following components in terms of molar percentage: Na2O 2~15%, K2O 3~20%, MgO 1~25%, CaO 5~30%, B2O3 8~50%, P2O5 1~18%, SiO2 5~35% and SrO 2~30%. The glass is prepared through a melting-ice water quenching-milling-screening process. The glass utilizes the synergistic effect of magnesium and strontium to regulate the release of boron ions, and the degradation rate is matched with bone regeneration; the glass has high bioactivity, good cell compatibility and excellent osteogenesis promotion performance, and is suitable for dental, orthopedic and plastic surgery bone repair materials, especially suitable for rapid bone regeneration and osteoporotic bone defect scenes, and solves the problem of cytotoxicity caused by the burst release of the existing boron.

This invention relates to a rapid screening method for bone-repairing surface structures, belonging to the field of bone repair materials technology. It aims to solve the technical problems of low efficiency and low individual / group adaptability of traditional screening methods. This invention constructs a titanium dioxide nanotube array with a continuous gradient diameter of 0-200 nm using a four-electrode bipolar electrochemical method. Immunofluorescence staining of key bone repair factors BMP-2, RUNX2, OPN, and OCN, combined with panoramic scanning analysis, is used to normalize the average fluorescence intensity of each of the four factors and then sum them to obtain a comprehensive osteogenic coefficient. Based on this coefficient, the optimal bone-repairing surface structure corresponding to bone marrow mesenchymal stem cells (BMSCs) from different age groups or different pathological conditions (such as osteoporosis, osteoarthritis, etc.) can be precisely located. This invention achieves high-throughput screening covering multiple surface structures in a single experiment, providing an efficient technical path for the precise and personalized design of bone repair materials, and has significant clinical translational value.

The invention provides traditional Chinese medicine granules for improving osteoporosis as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: performing deep eutectic solvent extraction and purification on codonopsis pilosula, scalded rhizoma drynariae, herba epimedii and eucommia ulmoides to prepare a traditional Chinese medicine extract, and performing water extraction, dialysis and drying on cornu cervi pantotrichum to prepare cornu cervi pantotrichum protein peptide; performing water extraction and alcohol precipitation on cistanche to prepare cistanche polysaccharide; hydroxyapatite is modified by citric acid, sodium dihydrogen phosphate and beta-cyclodextrin, calcium salt, alpha-calciferol and vitamin D3 are included, the prepared product is uniformly mixed with a traditional Chinese medicine extract, cornu cervi pantotrichum protein peptide and cistanche polysaccharide, and the traditional Chinese medicine granules for improving osteoporosis are prepared. The prepared traditional Chinese medicine granules for improving osteoporosis can promote synthesis of collagen and bone matrix protein, inhibit bone resorption, promote bone remodeling and enhance the activity of osteoblasts, have a very good improvement effect on symptoms such as osteoporosis and fracture, and have a wide application prospect.

A bone anchor, an implantation handle, and an implantation system are provided. The anchor includes an anchor body with axially extending through-lumen and, from its proximal toward its distal end, including an upper, middle, and lower section. The outer diameter of the middle section is smaller than that of the upper section. The upper section tapers conically and has a first helical thread. A recess in the distal face of the upper section is in communication with the lumen. The middle section has a cylindrical external thread, and the lower section has an open-work thread formed by hollow thread flanges. The cylindrical thread and the open-work thread together define a second helical thread. Multiple reinforcing ribs are disposed on the inner side of the open-work thread around the lumen. This construction reduces the risk of anchor pull-out in osteoporotic bone.

The utility model provides a high-stability bone extension bridging combined internal fixing device, and belongs to the technical field of bone extension. Comprising a first bone block and a second bone block, the plurality of fixing blocks are respectively arranged on the first bone block and the second bone block; the plurality of guide rods are arranged on the fixed block and are symmetrically arranged at the two ends of the fixed block; the fixing screw is arranged on the fixing block and is used for fixing the fixing block and a bone block; the fixing screw is provided with a screw body and a nut. By arranging the fixing screw, the fine threads at the bottom are beneficial to entering relatively loose sclerotin initially, the contact area and anchoring force with bone tissue can be increased along with the gradual thickening of the threads, and compared with a traditional single-thread screw, the fixing stability in an osteoporotic bone block can be effectively improved; and the loosening risk caused by insufficient friction force is reduced.

This invention relates to the field of fracture risk prediction technology, and particularly to a method and system for constructing a fracture risk prediction model for diabetic osteoporosis. The method involves collecting bone metabolism data and hip and spine bone density computed tomography images from diabetic patients to construct a multi-dimensional three-dimensional model of bone tissue microstructure. Based on this model, blood glucose fluctuation-bone strength mapping is performed to calculate the trabecular fragility increment and identify the pre-gradient feedback characteristics of brittle mechanical signals to obtain brittle mechanical pre-gradient feedback data. Phase shift analysis of bone stress signals is then performed on the above data to extract phase shift feature data and construct a fracture risk prediction model for diabetic osteoporosis. Finally, dynamic assessment and remote monitoring of the patient's fracture risk are conducted. This method integrates three-dimensional bone microstructure modeling, blood glucose fluctuation impact analysis, and artificial intelligence prediction, improving the early warning accuracy of diabetic osteoporotic fractures.

The invention belongs to the technical field of medical biological materials, and discloses a drug-loaded porous pure zinc scaffold as well as a preparation method and application thereof. The drug-loaded porous pure zinc scaffold comprises a porous pure zinc scaffold, the porous pure zinc scaffold is loaded with a traditional Chinese medicine composition; the porous pure zinc scaffold is of a periodic straight rod lattice structure, the rod diameter is 300 microns, and the pore diameter is 400 microns; the traditional Chinese medicine composition comprises eucommia ulmoides, dragon's blood, fructus psoraleae, scorpio, rhizoma drynariae and prepared rehmannia root. According to the drug-loaded porous pure zinc scaffold, mechanical supporting and bone conduction are achieved through structural bionic design, pure zinc is degraded to release zinc ions to achieve the osteogenesis, anti-inflammatory and antibacterial effects, the traditional Chinese medicine composition regulates bone metabolism and the microenvironment in a multi-target mode, and the three components cooperate to construct a scaffold-ion-drug three-in-one bone repair system; the method is especially suitable for bone defects caused by osteoporosis, osteoarthritis, bone tumors, traumatic fractures and the like, and has a wide clinical application prospect.

The invention relates to a medical image processing system and method, and belongs to the technical field of image processing, and the system comprises an acquisition module which is used for acquiring a bone X-ray image based on equipment, and transmitting the acquired bone X-ray image to a preprocessing module based on an image interface; the preprocessing module is used for preprocessing the bone X-ray image based on an RISC-V module to obtain a target image conforming to an osteoporosis neural network model Osteo-CNN, and sending the target image to the prediction module; the prediction module is used for predicting the target image based on an osteoporosis neural network model Osteo-CNN to obtain a prediction result, the prediction result comprises the existence or absence of osteoporosis and the risk level of osteoporosis, and the prediction result is sent to the report generation module; and the report generation module is used for generating a structured report based on the prediction result and displaying the structured report based on the display interface.

The invention discloses an application of an expression inhibitor of a muscular blind splicing regulator 1 (MBNL1) in promoting osteogenic differentiation and bone formation of bone marrow mesenchymal stem cells (BMSCs), and particularly discloses an application of the expression inhibitor of the MBNL1 in promoting osteogenic differentiation and bone formation of the BMSCs (bone marrow mesenchymal stem cells). According to the present invention, the in-vitro experiment results prove that the si-MBNL1 transfection on the BMSCs can promote the formation of osteoblast calcium nodules, the activity of the marker enzyme ALP, and the expression of the osteogenic related factor Runt related transcription factor 2 (RUNX2), the ALP, the osteopontin (OPN) and the osteocalcin (OCN), and can improve the osteogenic differentiation ability of the BMSCs, such that the si-MBNL1 transfection on the BMSCs can provide the osteogenic differentiation ability of the BMSCs, and further provide the application of the si-MBNL1 transfection on the BMSCs, and the application of the si-MBNL1 transfection on the BMSCs to the osteogenic differentiation of the BMSCs, according to the invention, an adeno-associated virus 9 (AAV9) carrier is used for constructing an sh-MBNL1 regulation technology as an MBNL1 expression inhibitor, and in-vivo experiments prove that the sh-MBNL1 expression inhibitor can be used for repairing estrogen deficiency type osteoporosis bone loss and promoting fracture healing. According to the invention, a breakthrough direction is provided for clinical early warning of osteoporosis, and a new technical means is provided for clinical osteoporosis or gene therapy for promoting fracture healing.

The present application relates to a kind of degradable cationic polymer with bone targeting function and its preparation method and application, specifically, the degradable cationic polymer with bone targeting function provided in the present application can effectively load nucleic acid (such as mRNA, miRNA, DNA), and the encapsulation efficiency is more than 90%, and stable nanoparticles are formed by electrostatic interaction, protect nucleic acid from degradation, while having the characteristics of slowing down particle positive electricity.This carrier shows better stability compared with LNP, supports long-term storage at low temperature.Its bone targeting is clear, promotes the accurate delivery of nucleic acid to bone tissue, enhances the efficacy and reduces side effects, while showing biocompatibility for osteoblasts without toxicity.The application range is wide, covering osteoporosis, osteoarthritis, bone tumor treatment and gene and stem cell therapy.Experiments have verified that the nanoparticles formed by the carrier and eGFP mRNA have high transfection efficiency, and the combination with miRNA effectively inhibits breast cancer bone metastasis, highlighting its therapeutic potential.

The invention provides a PLGA (poly (lactic-co-glycolic acid)-PEG (polyethylene glycol)-phosphonate polymer, a bone targeting nanoparticle as well as a preparation method and application of the bone targeting nanoparticle. According to the preparation method of the PLGA-PEG-phosphonate polymer, no catalyst is needed to participate in the reaction, the reaction can be carried out at room temperature, and the preparation method is a green chemical reaction method and is more suitable for large-scale process production requirements; the bone targeting nanoparticle is formed by self-assembly of the PLGA-PEG-phosphonate polymer and PLGA-PEG, the nanoparticle can be specifically combined with hydroxyapatite in bone tissue to realize targeting of the bone tissue, and the nanoparticle can be used as a drug carrier to deliver drugs to the bone tissue, so that the bone targeting nanoparticle has a good application prospect. And bone tissue related diseases such as osteoporosis, bone cancer and osteoarthritis can be treated.

The application provides a double-layer stent and a preparation method thereof, which comprises the following steps: dissolving L-polylactic acid in a solvent, adding calcium salt to the L-polylactic acid, and mixing to obtain a precursor solution; then electrospinning the precursor solution to obtain a fiber membrane; drying the fiber membrane to obtain a fiber layer of the double-layer stent; dissolving polyvinyl alcohol, gelatin and polyamide in a binary solvent, adding barium titanate to the binary solvent, and mixing to obtain a hydrogel solution; placing the fiber membrane at the bottom of a mold, then placing the hydrogel solution in the mold, and then performing freeze-crosslinking to shape the hydrogel in the double-layer stent; and finally immersing the hydrogel in the double-layer stent after demolding in salt water to obtain the double-layer stent. The fiber membrane and the hydrogel solution are combined through freeze-crosslinking to obtain the double-layer stent, and the double-layer stent can accelerate the formation of new bone when applied to osteoporotic bone defects and under the stimulation of ultrasonic waves.

The invention discloses sustained-release oral powder for treating osteoporosis and a preparation method of the sustained-release oral powder. The sustained-release oral powder comprises 2 parts of a supramolecular metal organic framework material sMOF, 0.8-1.0 part of microcrystalline cellulose and 0.1-0.2 part of magnesium stearate. Wherein the supramolecular metal organic framework material sMOF is formed by coordination self-assembly of Sr < 2 + >, Mg < 2 + >, alendronate (ALN), 1, 6-fructose diphosphate sodium salt (FDP) and casein phosphopeptides (CPP). The powder has excellent treatment effect, biocompatibility and slow release characteristic, can effectively promote osteoblast differentiation, inhibit osteoclast activity and reverse bone mineral density decline, and is suitable for treatment of bone diseases such as osteoporosis and fracture.

The application provides a kind of polyarylether sulfone ketone surface biomimetic mineralization hydrogel coating, its preparation method and application in bone repair.The biomimetic mineralization hydrogel coating is obtained by simulating body fluid mineralization of hydrogel coating, and apatite layer is formed in the interior and surface of hydrogel coating, the hydrogel coating includes polymer network and calcium phosphate oligomer uniformly dispersed therein, which is anchored to the surface of polyarylether sulfone ketone base.The introduction of calcium phosphate oligomer effectively solves the problem of inorganic phase agglomeration and improves the uniformity of mineralization.The mineralized hydrogel coating can significantly promote the expression of osteoblast MC3T3-E1 osteogenic activity and inhibit the differentiation of RAW264.7 cells into osteoclasts.The biomimetic mineralization hydrogel coating described in the application has dual biological activity of promoting bone formation and inhibiting bone resorption, and is suitable for surface modification of polyarylether ketone bone implants, and has wide application prospect in the field of osteoporotic bone defect repair.

GREM2 compositions and formulations thereof for treating or preventing one or more symptoms of a disease associated with loss of bone mineral density are described. Recombinant GREM2 has been demonstrated to be capable of reducing bone resorption, lowering bone transition markers, and increasing bone strength. Methods of using the GREM2 compositions and formulations are also provided. Preferably, the pharmaceutical formulation containing GREM2 is administered by a parenteral route to prevent or alleviate one or more symptoms of osteoporosis, bone mass reduction, bone fracture and / or bone cancer.