26 results about "Osteolysis" patented technology

The invention discloses application of rehmannia extracellular vesicles in preparation of a medicine for improving breast cancer bone metastasis bone injury, and belongs to the technical field of biological medicine. The preparation method comprises the following steps: cleaning the root tuber of a fresh rehmannia plant, adding a PBS (Phosphate Buffer Solution), crushing, and filtering by gauze to remove large-particle impurities; performing high-speed centrifugation on the filtrate to remove insoluble residues, then performing ultra-high-speed centrifugation to collect precipitates, re-suspending the precipitates, purifying the precipitates by adopting PEG (Polyethylene Glycol), and centrifugally collecting the precipitates, thereby obtaining the rehmannia extracellular vesicles. The rehmannia extracellular vesicles can effectively target bones, promote proliferation of bone-related cells and remarkably reduce bone injury caused by bone metastasis of breast cancer; bone protection treatment is carried out on breast cancer bone metastasis through the Chinese herbal medicine vesicles sourced from rehmannia, a safe and effective treatment scheme is provided for improving the pathological osteolysis problem caused by breast cancer bone metastasis, a new thought and technical support are provided for prevention and treatment of tumor bone metastasis related diseases, and good clinical transformation prospects are achieved.

The invention provides a Falcarindiol derivative, preparation thereof and application of the Falcarindiol derivative in preparation of medicines for preventing and / or treating bone diseases. The structural general formula of the Falcarindiol derivative is as shown in formula I in the specification. Experimental results show that the Falcarindiol derivative can significantly inhibit osteoclast differentiation, and is expected to be used for preparing novel drugs for preventing and / or treating osteoporosis, osteoporosis fracture, osteolysis, periprosthetic osteolysis, tumor bone metastasis, inflammatory bone loss, periodontitis inflammatory bone resorption and other bone diseases. # imgabs0 #

The present invention provides pharmaceutical compositions and methods for use in accelerating bone repair and growth and attenuating inflammation-induced osteolysis. In particular, the present invention provides pharmaceutical compositions comprising a peptide comprising the sequence YGFGG for use in treating osteolysis. The present invention further provides pharmaceutical compositions comprising a peptide comprising the sequence YGFGG and CBD for use in accelerating bone repair and / or bone growth in a subject having a bone fracture.

The invention discloses myricetin-loaded bone-targeted magnetic composite nanoparticles and a preparation method thereof. The preparation method comprises the following steps: step 1, preparing nanoparticles taking ferroferric oxide as an inner core; step 2, preparing magnetic mesoporous silica nanoparticles with ferroferric oxide as an inner core according to the nanoparticles prepared in the step 1; step 3, performing amination modification treatment on the surfaces of the magnetic mesoporous silica nanoparticles prepared in the step 2 to obtain magnetic mesoporous silica modified nanoparticles; step 4, preparing a ketal thiol-polyethylene glycol-aspartic acid hexapeptide molecule; and step 5, loading myricetin on the magnetic mesoporous silica modified nanoparticles prepared in the step 3, and blocking myricetin by using the ketal thiol-polyethylene glycol-aspartic acid hexapeptide molecules prepared in the step 4 after loading. The invention further discloses application of the compound in image tracing in osteolysis and treatment drugs.

The utility model relates to the technical field of medical instruments, in particular to a positioning device for assisting reduction and fixing acromioclavicular dislocation, which comprises a base plate, an acromion end positioning plate is arranged at one end of the base plate, and a clavicle end positioning block is arranged at the other end of the base plate. Holes are formed in the acromion end positioning plate, acromion end fixing guide needle sleeves are fixedly connected to the positions corresponding to the holes, a plurality of holes are formed in the base plate, and a first coracoshoulder ligament fixing guide needle sleeve, a second coracoshoulder ligament fixing guide needle sleeve and a clavicle end guide needle fixing sleeve are fixedly connected to the positions corresponding to the holes respectively. The positioning device can achieve accurate positioning, damage to blood vessels, nerve tracts and surrounding tissues below the coracoid process is avoided, and operation bleeding is reduced. Due to the fact that the individualized bone tunnel is established, the position where the belt carrier titanium plate is placed is appropriate, the osteolysis phenomenon is reduced, and meanwhile the risk of coracoid fracture is reduced. According to the operation method adopting the positioning device, the operation difficulty is remarkably reduced, the operation time is shortened, and the learning curve of young physicians is reduced.

The invention discloses an RIP3-mediated osteoarthritis animal model as well as a construction method and application thereof. The construction method comprises the following steps: delivering an effective dose of a genetic tool (such as an adenovirus vector) capable of expressing RIP3 protein into a knee joint subchondral bone area of an experimental animal, and realizing continuous overexpression of RIP3 through regular operation, so as to induce pathological characteristics highly similar to human osteoarthritis. The model stably shows typical OA phenotypes such as limb pain sensitization, subchondral bone loss and bone microstructure degeneration, osteoclast number increase and overlying cartilage degeneration. The invention reveals that the natural process of occurrence and development of the osteoarthritis can be directly simulated through specific overexpression of the RIP3 in the subchondral bone for the first time, and a brand-new and valuable tool is provided for researching the pathogenesis of the osteoarthritis and screening and evaluating medicines and therapies for treating the osteoarthritis.

The invention provides application of wogonoside in preparation of a medicine for preventing and treating inflammatory osteolysis, and belongs to the technical field of bone disease treatment, wogonoside has an inhibiting effect on RANKL-induced osteoclast generation and bone resorption, and MAPK and NF-kB signal channels related to osteoclast differentiation are detected through WB and PCR experiments. The wogonoside has an inhibiting effect on osteoclast differentiation, bone resorption function and bone loss of LPS-induced inflammatory osteolysis mice, and the effect is possibly related to inhibition of RANKL-mediated ROS level and a downstream MAPK signal channel. Therefore, the invention possibly provides a new treatment thought for preventing inflammatory osteolysis diseases.

The application discloses a preparation method of a 3D printing hip joint stress relief heat treatment double-gradient bone support, and the method comprises the following steps: acquiring image data by performing three-dimensional scanning on a bone defect by means of a CT scanning device, designing the size and shape of a transplantable bone, designing a concave hexagonal support structure with gradient porosity by means of UG three-dimensional drawing software, and gradually transitioning from a Ti-35Nb-5Ta-7Zr alloy with good biocompatibility and a low elastic modulus to a Co-Cr-Mo alloy with good wear resistance and corrosion resistance, improving metallurgical incompatibility, obtaining better interface combination, minimizing bone resorption and aseptic loosening caused by wear, prolonging the service life of an implant, and performing annealing heat treatment on the functional support after printing to eliminate residual stress and depositing a HA coating to enhance cell activity.

According to the antibacterial coating and the preparation method provided by the invention, the degradation rate of the magnesium alloy can be remarkably reduced by the composite coating formed by combining the micro-arc oxidation film layer and the copper-doped ZIF-8 coating, copper is doped when the ZIF-8 coating is prepared, and the ZIF-8 coating can be endowed with relatively strong antibacterial performance aiming at gram-positive bacteria and gram-negative bacteria; the coating material is mainly applied to the surface of a magnesium alloy implantation instrument, and a stable surface structure with excellent biocompatibility is formed by controlling the degradation rate of magnesium alloy, so that subcutaneous gas accumulation generated by rapid degradation and hemolysis and bone dissolution caused by a local strong alkaline environment are avoided; severely, the problems that an implantation instrument loses fixing and supporting functions before tissue healing, and implantation fails are caused are solved; meanwhile, the early adhesion of bacteria and the formation of bacterial biofilms are effectively inhibited by utilizing the antibacterial function of the coating, so that the infection risk generated in the implanting and using processes of the implanting instrument is reduced.

The present invention relates to the technical field of orthopedic artificial joints, and in particular, to a metal total knee artificial joint and a method for preparing a metal total knee artificial joint. The metal total knee artificial joint comprises a femoral condyle prosthesis and a tibial plateau prosthesis. Both the femoral condyle prosthesis and the tibial plateau prosthesis are made of 3D printing of zirconium-niobium alloy. The two can be slidably matched, and the sliding contact surface of the femoral condyle prosthesis and the tibial plateau prosthesis is provided with a metal-ceramic interface; the integration interface of the femoral condyle prosthesis and the tibial plateau prosthesis with the external bone body is a 3D-printed trabecular integration interface. The metal total knee artificial joint eliminates the tibial plateau pad prosthesis, and through the metal-ceramic interface, it can reduce the amount of osteotomy of the tibial plateau and optimize the wear of the friction interface, thereby avoiding the risk of osteolysis caused by debris generated by wear, which in turn causes the prosthesis to loosen. It can also improve the bone integration efficiency of the interface between the prosthesis and the bone tissue, which is beneficial to the stability of the prosthesis in the later stage of implantation.

The invention discloses application of an ECRG4 gene in preparation of drugs for prevention, treatment, adjuvant treatment, alleviation or alleviation of bone imbalance related diseases, and relates to the technical field of biology. By overexpressing the ECRG4 gene, the effects of prevention, treatment, adjuvant therapy, alleviation or alleviation of bone imbalance related diseases are achieved, osteoblast differentiation is promoted and / or osteoclast formation is inhibited, and the overexpressed ECRG4 gene has a nucleotide sequence as shown in SEQ ID NO: 1. It is found for the first time that the ECRG4 gene can be used as an effective target for prevention, treatment, adjuvant treatment, and alleviation or alleviation of bone imbalance related diseases. Overexpression of the ECRG4 gene can effectively promote osteogenic differentiation to facilitate bone regeneration and inhibit osteoclast differentiation to facilitate bone resorption, which reveals that the ECRG4 gene has potential application in bone-related diseases, including but not limited to fracture healing, osteoporosis, osteolysis and the like, and has broad application prospects. And a new direction is provided for prevention, treatment, adjuvant treatment and alleviation or alleviation of bone imbalance related diseases.

The present application relates to the application of circPDE5A inhibitor in the preparation of drugs for treating aseptic loosening of artificial joints. The present application confirms that circPDE5A has a positive correlation with the occurrence of aseptic loosening. And through RNA Pull-Down experiment and mass spectrometry (MS) analysis, it is confirmed that circPDE5A can affect the regulation of LACC1 signal pathway by interacting with LACC1, and then affect the inflammatory response of macrophages. In addition, through in vivo animal experiments, the results of mouse skull osteolysis model show that the injection of lentivirus for knocking down circPDE5A can reduce the osteolysis induced by Ti particles. In general, the present application provides a new idea and theoretical basis for the research and development of drugs for treating aseptic loosening of artificial joints, and has important clinical value and social significance.

The invention relates to the technical field of nanomedicine and functional material crossing, and discloses a bimetallic nanoparticle and a preparation method and application thereof.The bimetallic nanoparticle comprises metal A and metal B and is coated with protein polypeptide substances, and the preparation method comprises the steps that the protein polypeptide substances are evenly suspended in water, a metal solution and a carbonate solution are added, and then the pH of the solution is adjusted to be alkaline; the preparation method comprises the following steps: adding a proper reaction temperature, a proper time and a reaction system, dialyzing, freezing and drying to obtain bimetallic nanoparticles, using an ultrafiltration centrifuge tube to obtain the bimetallic nano quantum dots, and applying the bimetallic nanoparticles to regeneration and repair of tumor and tissue damage, osteolysis related to prosthesis, and treatment of cardiovascular and metabolic diseases. The bimetallic nanoparticles have the advantages of good repeatability, uniform particle size distribution, controllable structure, multiple functions and excellent biocompatibility, solve the problem of poor targeting property of existing drugs, are suitable for the fields of precise treatment and drug delivery, and also have application prospects in many fields of daily chemicals, batteries, environmental protection and the like.

Hollow hydroxyapatite microparticles are provided for sustained delivery of an agent that provides osteoprotegerin anti-osteolytic activity for treatment of post-orthodontic relapse and bone disorders. In particular, methods of using such microparticles for post-orthodontic tooth stabilization and bone regeneration and repair are provided.

The application discloses an acetabular cup for acetabular surgery, which adopts a multilayer structure, namely a first acetabular cup and a second acetabular cup, and the two are connected through an acetabular cup spacer block, the acetabular cup spacer block can prevent the relative movement between the first acetabular cup and the second acetabular cup, improve the stability and wear resistance of the acetabular cup, prolong the service life of the acetabular cup, the first acetabular cup and the second acetabular cup are both trabecular bone structure and are both hollow hemispheres, and have high porosity, so that the biomechanical adaptability of the acetabular cup can be improved, the growth of bone tissue and the fixation of the acetabular cup can be promoted, the stress concentration and osteolysis of the acetabular cup can be reduced, the biocompatibility and corrosion resistance of the acetabular cup can be improved, and a plurality of groups of internal thread base holes are formed in the first acetabular cup, the second acetabular cup and the acetabular cup spacer block, so that the installation and fixation of the acetabular cup are facilitated, and thus the accurate positioning and close connection of the acetabular cup are realized.

This invention discloses a method and prosthesis for connecting a porous surface structure and a substrate using a thermoplastic material. A layer of thermoplastic polymer material is placed in the gap between the porous surface structure and the substrate, allowing the porous surface structure to be tightly bonded to the porous structure on which the substrate is located or to a pre-connected porous structure on the substrate. This improves stress shielding and solves the problem of postoperative osteolysis. The prosthesis of this invention has excellent bone ingrowth performance, ensuring that the strength of the substrate is not substantially affected, guaranteeing the basic strength of the connection structure, and optimizing the overall composite structure stiffness, effectively reducing the risk of stress shielding.

The application discloses a knee joint prosthesis system and a preparation method, wherein the femoral condyle prosthesis, the tibial plateau pad prosthesis, the tibial plateau prosthesis and the patellar prosthesis are made of zirconium niobium alloy, so that the sliding friction contact surfaces of the tibial plateau pad prosthesis, the patellar prosthesis and the femoral condyle prosthesis are metal ceramic interfaces formed by oxidation of zirconium niobium surfaces, the wear rate is lower, the active wear of the joint prosthesis can be effectively reduced, the service life of the joint prosthesis is prolonged and the failure rate is reduced, meanwhile, the risk of osteolysis caused by debris generated by wear of a traditional prosthesis due to an ultra-high molecular weight polyethylene tibial plateau pad prosthesis, and further triggering the risk of loosening of the prosthesis, can be avoided. Moreover, the bone integration interfaces of the femoral condyle prosthesis, the tibial plateau prosthesis and the patellar prosthesis are zirconium niobium alloy trabecular structures, so that the zirconium niobium alloy trabecular structures have excellent biocompatibility, are convenient to combine with the bone tissue of a patient and can improve the bone integration efficiency of the frame body and the bone tissue.

The invention relates to the field of pharmacy, in particular to application of luteolin in preparation of a medicine for treating periprosthetic osteolysis. Experiments show that luteolin has an obvious inhibition effect on osteolysis induced by titanium particles by enhancing expression of FOXO3A, reducing oxidative stress of MC3T3-E1 cells, recovering osteogenesis functions of BMSCs and relieving osteolysis and bone loss induced by the titanium particles, and the luteolin can be used for preparing a medicine for treating osteolysis induced by the titanium particles. The method can be used as a new means for medicine intervention of osteolysis around the prosthesis after artificial prosthesis replacement.

The invention discloses an automatic identification method for osteolysis around a prosthesis after total hip replacement, and belongs to the technical field of medical image processing.The method is achieved through an osteolysis focus identification and prediction model, in the model, image data of a patient after total hip replacement is converted and mapped, and an image matrix is obtained; obtaining an initial partition map of the image matrix through the anatomical partition template; determining a weight matrix of the initial partition map according to preset weights of different anatomical partitions, multiplying the weight matrix by the image matrix to obtain a partition guide enhanced image matrix, inputting the partition guide enhanced image matrix into a feature extraction module, inputting extracted micro texture features and macro features into a Sigmoid gating dynamic fusion module, and outputting the Sigmoid gating dynamic fusion module. And a target bounding box and target confidence are obtained through the fusion features, the intermediate layer feature map and the YOLOv8 recognition module, a staging result is obtained based on the fusion features, clinical data and a staging prediction module, and visual recognition information is further obtained.

This application relates to the field of orthopedic artificial joint design and manufacturing technology, and in particular to a metal-ceramic total shoulder joint prosthesis system and its preparation method. The metal-ceramic total shoulder joint prosthesis system includes a humeral stem prosthesis, a tapered adapter prosthesis, a humeral head prosthesis, and a glenoid fossa prosthesis connected sequentially. The humeral head prosthesis and the tapered adapter prosthesis are both formed using 3D zirconium-niobium metal printing or forging. The humeral stem prosthesis and the glenoid fossa prosthesis are integrally formed using 3D printed zirconium-niobium alloy. A metal-ceramic interface layer formed by the oxidation of the zirconium-niobium surface is provided at the relative motion contact surface of the humeral head prosthesis and the glenoid fossa prosthesis. The metal-ceramic total shoulder joint prosthesis system provided by this application has advantages such as longer service life, high wear resistance, low wear rate, no osteolysis, and no prosthesis loosening.

The present invention discloses the use of Rehmannia glutinosa extracellular vesicles in the preparation of drugs for improving bone damage caused by bone metastasis of breast cancer, and belongs to the field of biomedicine technology. Take the tuberous roots of fresh Rehmannia glutinosa plants, wash them, add PBS buffer, crush them, and filter them through gauze to remove large particle impurities; the filtrate is subjected to high-speed centrifugation to remove insoluble residues, and then ultra-high-speed centrifugation is performed to collect the precipitate, and the precipitate is resuspended and purified with PEG, and the precipitate is collected by centrifugation to obtain the Rehmannia glutinosa extracellular vesicles. Rehmannia glutinosa extracellular vesicles can effectively target bones, promote the proliferation of bone-related cells, and significantly reduce bone damage caused by bone metastasis of breast cancer; bone protection treatment of breast cancer bone metastasis is performed by using Chinese herbal vesicles derived from Rehmannia glutinosa, which provides a safe and effective treatment plan for improving the problem of pathological osteolysis caused by breast cancer bone metastasis, provides new ideas and technical support for the prevention and treatment of diseases related to tumor bone metastasis, and has good prospects for clinical transformation.

The present invention relates to uses of a combination therapy of bone morphogenetic protein-2 and denosumab in spinal fusion surgery. When bone morphogenetic protein-2 and denosumab are used in combination during spinal fusion surgery as in the present invention, side effects frequently occurring due to spinal fusion surgery, such as osteolysis, inflammation, wound dehiscence, and bleeding, can be effectively prevented and treated.

The invention belongs to the field of biotechnology and medicine, and discloses application of cholesterol hydroxylase CH25H intervention polypeptide in preparation of osteoporosis and osteolysis treatment products. The polypeptide fragment containing the TRAF6 binding site designed by the invention is specifically bound with CH25H so as to destroy the interaction between CH25H and TRAF6, thereby effectively inhibiting OC differentiation and bone resorption functions. Theoretical and technical bases are provided for developing novel treatment strategies for preventing and treating bone metabolic diseases such as osteoporosis (OP) and osteolysis (OL).

The application discloses a new use of table berberine and an experimental method for inhibiting breast cancer cell-induced osteoclast differentiation. The application proves for the first time that table berberine does not inhibit the proliferation of osteoclast precursor cells at a higher concentration (25 μM), and has higher biological safety. In addition, table berberine has a significant inhibitory effect on breast cancer-induced osteoclast formation and metastatic breast cancer-induced osteolysis.

The invention provides a Falcarinphthalide derivative, preparation thereof and application of the Falcarinphthalide derivative in preparation of medicines for preventing and / or treating bone diseases. The structural general formula of the Falcarinphthalide derivative is as shown in formula I in the specification. Experimental results show that the Falcarinphthalide derivative can significantly inhibit osteoclast differentiation, and is expected to be used for preparing novel drugs for preventing and / or treating osteoporosis, osteoporotic fracture, osteolysis, periprosthetic osteolysis, tumor bone metastasis, inflammatory bone loss, periodontitis inflammatory bone resorption and other bone diseases. # imgabs0 #

The invention relates to an application of a circPDE5A inhibitor in preparation of a medicine for treating sterile looseness of an artificial joint. According to the present invention, the positive correlation between the circPDE5A and the sterile loosening is determined; through RNA Pull-Down experiments and protein mass spectrometry (MS) analysis, it is clear that circPDE5A can affect regulation and control of an LACC1 signal channel through interaction with LACC1, and then the inflammatory response of macrophages is affected. In addition, through in-vivo animal experiments, the result of a mouse skull osteolysis model shows that the injection of lentivirus for knocking down circPDE5A can relieve osteolysis induced by Ti particles. In general, the invention provides a new thought and theoretical basis for research and development of medicines for treating sterile looseness of artificial joints, and has important clinical value and social significance.