48 results about "Targeted nanoparticles" patented technology

Compositions and methods are provided for modifying the contents of exosomes released by target cells. In one embodiment cell targeted nanoparticles are used to deliver interference RNAs to the target cells which alter the packaging of exosomes released by the target cells.

The application belongs to the technical field of targeted drugs, and discloses a carrier, a targeted nanoparticle and a preparation method and application thereof. The carrier disclosed by the application comprises darpin grafted with a dye containing a terminal carboxyl group. The targeted nanoparticle comprises the carrier and a CO releasing molecule, and the carrier coats the CO releasing molecule to form a core-shell structure. The targeted nanoparticle is based on darpin, the grafting of the dye containing the terminal carboxyl group is performed to construct the carrier, and then the carrier coats the CO releasing molecule, wherein the carrier has a targeting effect, and the photothermal effect of the carrier can realize the NIR laser controlled CO releasing or free radical stimulation, and can also target the controllable CO releasing, so that the double response releasing of exogenous CO and endogenous CO is realized, and the application has a wide application prospect.

The application belongs to the field of medical materials, and particularly relates to a metal core enhanced double-barrier piezoelectric nano system and a preparation method and application thereof. The preparation method comprises the following steps: first, a core-shell structure of'metal@mesoporous titanium dioxide@mesoporous barium titanate' is prepared through a step-by-step reaction, and then the target nanoparticles are obtained through lipid wrapping. The system innovatively constructs a double-barrier structure, can self-trigger active oxygen under a physiological pressure of 4 mmHg, realizes endogenous pressure self-driven treatment of malignant ascites, has the advantages of low pressure, high catalytic efficiency, long-term structural stability and good biocompatibility, solves the problems that existing treatment methods are highly invasive and depend on exogenous stimulation, and provides a new safe and efficient technical scheme for the treatment of malignant ascites.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The present application relates to the technical field of targeted nanoparticles, and particularly relates to a mitochondria-targeting autophagy-inhibiting polymer and a preparation method thereof, a nano delivery particle and a preparation method and application thereof. A high molecule TPP-PEG-SH (TPS) containing a triphenylphosphonium group (triphenylphosphonium, TPP) is coupled with a mitochondria autophagy inhibitor Mdivi-1 through a disulfide bond to construct a polymer TPSM with mitochondria targeting ability and GSH responsiveness, and further co-assembled with a photosensitizer IT-4Cl to form a nano delivery system TPSM@IT-4Cl. The nano delivery system has mitochondria targeting ability, promotes continuous accumulation of oxidative stress at a tumor site, and can realize responsive drug release in a high-concentration GSH tumor microenvironment.

The invention relates to the technical field of biological medicine preparations, in particular to application of targeted nanoparticles in treatment of osteoporosis. The targeted nano-particles CL-NPs (at) NEs are developed, CL can stimulate lipolysis of bone marrow adipocytes, osteogenic differentiation is promoted, and therefore the bone marrow microenvironment is improved; meanwhile, autologous neutrophil is used as a carrier to transport CL-NPs, bone marrow fat cells beta3AR are accurately targeted, and the osteoporosis microenvironment can be further improved. Experiments prove that CL-NPs (at) NEs stimulate a bone marrow fat cell beta3 adrenoceptor in a targeted manner, can effectively improve OP, has safety and has the potential to become a novel medicine for osteoporosis.

The invention relates to the field of drug delivery, in particular to a prediction method and device for nanoparticle assembly and a program product. Comprising the following steps: acquiring three-dimensional compound molecules of a target compound and a target nanoparticle size; serializing the topological structure of the three-dimensional compound molecule to obtain a topological sequence; obtaining a three-dimensional coordinate of each atom according to the sequence of the atoms in the topological structure to obtain a molecular conformation sequence; discretizing the size of the target nanoparticles to obtain a size sequence; integrating the topological sequence and the size sequence to obtain a first topological sequence, and integrating the molecular conformation sequence and the size sequence to obtain a first conformation sequence; and outputting the first topological sequence and the first conformation sequence to a neural network for feature extraction, and then performing prediction to obtain metal ions, organic compound additives and corresponding three-dimensional conformations of nanoparticles with specified sizes. The method has good application value.

The present invention provides: a method for preparing tissue-targeting nanoparticles, the method comprising a step for generating phospholipid nanoparticles, a step for generating mesenchymal stem cell-derived cell membrane nanoparticles, and a step for fusing the generated phospholipid nanoparticles and cell membrane nanoparticles to generate tissue-targeting nanoparticles; and tissue-targeting nanoparticles which are prepared by the method and comprise bisphosphonate-containing phospholipid nanoparticles and mesenchymal stem cell-derived cell membrane nanoparticles.

Provided herein are compositions, methods, and particles that include a peptide that targets placental chondroitin sulfate A (placental CSA), wherein the amino acid sequence of the polypeptide is as set forth in SEQ ID NO: 1 (EDVKDINFDTKEKFLAGLIVSFHEGKC).

The invention provides cardiac targeting nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The heart targeting nanoparticle provided by the invention contains the mesoporous silica nanoparticle, the myocardial targeting peptide and the SNX7 inhibitor, and can significantly improve the enrichment efficiency of a therapeutic drug at a heart part and reduce the exposure of the drug in non-target tissues, so that the heart function is effectively improved and the potential toxic and side effects are reduced; the nanoparticle system has excellent biocompatibility and stability, provides a new solution for precise treatment of heart failure, has wide clinical application prospects, and is expected to promote progress in the field of cardiovascular disease treatment.

The invention discloses a nanoparticle capturing method and related equipment, and the method comprises the following steps: uniformly covering a hydrogel solution on a sample liquid drop containing target nanoparticles on a glass slide to form a standard observation system; the hydrogel solution is temperature sensitive type hydrogel; determining a local area where the target nanoparticles are located in a standard observation system; starting a laser, controlling the laser to irradiate the local area, and regulating and controlling the laser power density of the laser, so that the temperature of the local area reaches and is maintained at the phase change temperature of the hydrogel solution; and adjusting laser parameters of the laser to generate stable hydrogel at the position of the target nanoparticle. According to the embodiment of the invention, the nano-particles can be captured by utilizing the characteristics of the temperature-sensitive hydrogel and combining a simple photo-thermal device, the influence caused by the limitation of capturing environment conditions or capturing sample properties is reduced, and the method can be widely applied to the technical field of nano-particle capturing.

This application discloses a tumor-targeting nanoparticle, its preparation method, and its application, belonging to the field of tumor drug technology. The tumor-targeting nanoparticle includes a drug and a delivery carrier loading the drug, wherein the drug is atorvaquinone. The delivery carrier is prepared by coupling an intermediate product with Sulfo-SMCC-BSA. The intermediate product is obtained by reducing a thiol-modified nucleic acid aptamer, wherein the nucleic acid aptamer is EGFR aptamer. This application utilizes a EGFR-targeting nucleic acid aptamer coupled with bovine serum albumin (BSA) to encapsulate atorvaquinone, successfully constructing a novel atorvaquinone nanomedicine. This improves the targeting ability of atorvaquinone and increases its water solubility, providing a new approach for the treatment of esophageal cancer with atorvaquinone nanomedicines.

The invention discloses preparation and application of macrophage targeting nanoparticles entrapped with a cell burial function accelerant. The medicine for enhancing the intercellular function of the macrophages is a liver X receptor stimulant, and the liver X receptor stimulant comprises at least one of T0901317, GW3965, Ouabagenin and LXR-623. The surface of the targeting nanoparticle is coated with a macrophage cell membrane, and meanwhile, a urokinase type plasminogen activator receptor (uPAR) targeting ligand is modified. The nanoparticle can be efficiently accumulated at an inflammatory focus part by utilizing a macrophage cell membrane, and the modified targeting ligand is used for targeting the macrophage with damaged cell burial function, so that the cell burial function of the macrophage is enhanced, the polarization of the macrophage to an anti-inflammatory phenotype is promoted, the release of anti-inflammatory and proinflammatory cytokines is regulated, and the anti-inflammatory effect of the nanoparticle is improved. And finally, the inflammatory microenvironment of a focus part is regulated and controlled, so that a good effect of treating autoimmune diseases is achieved.

This invention discloses a plant exosome-liposome composite targeted nanoparticle drug delivery system, its preparation method, and its applications. The nanoparticle drug delivery system comprises plant exosomes and liposomes, as well as a targeting polypeptide linked to the surface of the liposomes and an antioxidant drug encapsulated within the liposomes. The plant exosome-liposome composite targeted nanoparticle drug delivery system prepared by this invention can penetrate the blood-brain barrier, possesses the ability to actively target nerve cells, exhibits good drug carrier stability, and is not easily degraded by macrophages or the reticuloendothelial system. Combining the dual therapeutic advantages of the active substances carried by plant exosomes and the antioxidant drugs, it achieves both the regulation of autophagy in nerve cells and the inhibition of neuroinflammation, realizing multi-target therapy and providing a new approach and drug for the efficient treatment of Parkinson's disease.

The invention provides a lysozyme-based quercetin colon-targeted nanoparticle as well as a preparation method and application of the lysozyme-based quercetin colon-targeted nanoparticle. According to the invention, a quercetin-Fe < 2 + > metal polyphenol network (MPN) nano core is constructed by a solvent exchange method, and then the quercetin-Fe < 2 + > metal polyphenol network (MPN) nano core, positively charged egg white lysozyme and negatively charged pectin are subjected to electrostatic self-assembly in sequence to form the nano particle with a triple coating structure. The encapsulation rate of quercetin in the nano-particles ranges from 77% to 98%, the drug loading rate ranges from 13% to 32.2%, and the nano-particles have excellent gastrointestinal stability and colon targeting. High-fat model animal experiment results show that the nanoparticles have good lipid-lowering effect and oxidation resistance, and can protect intestinal structures and functions. The problems of poor stability and insufficient targeting in oral delivery of quercetin are effectively solved, and a green and efficient solution is provided for colon targeting delivery of polyphenol functional factors.

This invention relates to the field of granule preparation technology, and discloses a Coptis chinensis preparation for treating gastrointestinal damp-heat stagnation and its preparation method. First, the preparation components, including total alkaloids of Coptis chinensis, volatile oil of Aucklandia lappa, dry extract powder of excipient medicinal materials, chitosan, and pectin, are determined according to specific weight ratios. For different components, supercritical CO2 extraction, dynamic cyclic extraction combined with near-infrared online monitoring technology, and standardized water decoction process are used to accurately extract and prepare the preparation. Then, a chitosan-pectin nano-targeted delivery system is constructed, and colon-targeted nanoparticles are obtained by modifying colonic epithelial cell-targeting peptides. These nanoparticles are then obtained by vacuum freeze-drying and fluidized bed microsphere granulation. Simultaneously, the volatile oil of Aucklandia lappa is treated with β-cyclodextrin inclusion complex. Finally, the nanoparticles are mixed with the volatile oil inclusion complex, dry extract powder of excipient medicinal materials, enteric-coated excipients, and fillers to prepare a thermosensitive bio-ink. A thermosensitive gel framework preparation is then obtained using semi-solid extrusion 3D printing technology.

The invention relates to a supercritical anti-solvent-electrostatic spray coupling system which comprises a supercritical anti-solvent generation module, a material mixing module and an electrostatic atomization module which are sequentially connected through a connecting pipeline, and a time-space synchronization control module which is in communication connection with the supercritical anti-solvent generation module and the electrostatic atomization module. The space-time synchronous control module cooperatively controls the oscillating electric field and the pressure of the supercritical anti-solvent, so that the electric field jet breaking of the material is realized by the electric field force, and the absolute value of the difference value between the Taylor cone forming time and the supercritical anti-solvent diffusion time is controlled within a set range, so that the supercritical anti-solvent permeates into liquid drops within a millisecond-level time scale; the invention discloses a preparation method of brain-targeted nano-particles, which comprises the following steps: dissolving a solvent in a solvent to trigger a sudden drop effect of polarity of the solvent, so that solute is instantaneously crystallized, and finally a nano-suspension with a particle size of less than 200nm and uniform particle size distribution (PDilt: 0.2) is formed, is suitable for preparing brain-targeted nano-particles with high drug loading capacity and small particle size, and can effectively cross a blood-brain barrier (BBB) to realize continuous and green production.

This invention discloses a targeted nanoparticle for calcium overload therapy combined with photothermal therapy, specifically involving a lipid-modified cRGD-targeting, MMP-responsive, and pH-sensitive calcium carbonate nanoparticle loaded with indocyanine green (ICG). This invention uses pH-sensitive calcium carbonate, which can decompose and exfoliate within lysosomes, as the matrix material. It leverages cRGD's specific targeting of tumor cells, the tumor microenvironment-specific high expression of matrix metalloproteinases (MMPs) to induce the release of DPPA-1 within the tumor microenvironment, and the low pH characteristic within lysosomes to achieve a combined anti-tumor effect of phototherapy and immunotherapy.

This invention provides nanoparticles with plaque-targeting function, their preparation method, and their application in the preparation of anti-atherosclerotic drugs, belonging to the field of composite nanomaterials technology. The plaque-targeting nanoparticles provided by this invention comprise a core and a shell. The core is ZIF-8 nanoparticles loaded with neoindocyanine green, and the shell is arginine-modified polydopamine; the surface of the shell is modified with hyaluronic acid. The plaque-targeting nanoparticles provided by this invention can regulate lipid levels by simultaneously intervening in multiple pathways, providing anti-inflammatory effects, protecting damaged endothelial cells, and enabling low-temperature (42-45℃) photothermal therapy combined with anti-nlrp3 therapy. This treats atherosclerosis by regulating PCSK9 pathway-induced lipid accumulation and repairing damaged endothelial cells.

The invention discloses a multifunctional targeting hydrogel based on chiral polypeptide as well as a preparation method and application thereof, and the multifunctional targeting hydrogel based on chiral polypeptide comprises the following raw materials in parts by weight: targeting nanoparticles loaded with a TGF-beta inhibitor, chiral antioxidant polypeptide, a cross-linking agent and water. The multifunctional targeted hydrogel has the functions of MI early-stage efficient ROS removal and MI late-stage targeted fibrosis blocking, has the functions of remodeling the infarction area microenvironment, inhibiting scar tissue expansion and recovering cardiac muscle synchronous electrical conduction, and finally achieves coordination of hydrogel degradation kinetics and the heart repair process and multi-dimensional repair of damaged cardiac muscle.

The invention discloses a measurement method for obtaining a nanoparticle asymmetry factor signal through analog computation, relates to the technical field of nanoparticle measurement, and is applied to a measurement system for the nanoparticle asymmetry factor signal, and the measurement system for the nanoparticle asymmetry factor signal comprises a microscope and electromagnetic simulation software. The method comprises the steps that a two-dimensional image sequence of target nanoparticles is collected through an acquisition microscope, a three-dimensional model of the target nanoparticles is constructed according to the two-dimensional image sequence, and the target nanoparticles at least comprise two nanoparticles; in the electromagnetic simulation software, the spectral data corresponding to the three-dimensional model is obtained, and the asymmetric factor of the target nanoparticle is determined according to the spectral data, so that the measurement accuracy of the asymmetric factor signal of the nanoparticle is improved.

A polymeric macrophage-targeting nanoparticle delivery system comprising: an outer chitosan shell functionalized with 1,3-β-glucan and optionally at least one active compound; a hydrophobic polymer core comprising an immunomodulating agent and optionally at least one active compound; wherein where there is no active compound provided in the outer chitosan shell, there is an active compound provided in the hydrophobic polymer core and wherein where there is no active compound provided in the hydrophobic polymer core there is an active compound provided in the outer chitosan shell.

The invention provides a cathepsin K (CTSK) responsive polypeptide derivative and application thereof. The CTSK responsive polypeptide derivative provided by the invention has the following structure: Ar-L1-dipeptide linker-hydrophobic membrane permeation sequence-CTSK response sequence-bone targeting sequence, and can be self-assembled to form bone targeting nanoparticles. According to the CTSK responsive polypeptide derivative and the bone targeting nanoparticles obtained from the CTSK responsive polypeptide derivative disclosed by the invention, the combination of osteoclast and apoptotic body mediated bone regeneration is specifically eliminated by utilizing self-assembly triggered by CTSK, so that bone balance is recovered, and physiological bone remodeling is not destroyed.

The application provides a lysozyme-based quercetin colon-targeting nanoparticle and a preparation method and application thereof. 2+ A metal polyphenol network (MPN) nanocore is sequentially self-assembled with positively charged egg white lysozyme and negatively charged pectin through electrostatic self-assembly to form a nanoparticle with a triple-coating structure. The quercetin encapsulation rate of the nanoparticle ranges from 77% to 98%, the drug loading rate ranges from 13% to 32.2%, and the nanoparticle has excellent gastrointestinal stability and colon targeting. The results of high-fat model animal experiments show that the nanoparticle has good lipid-lowering effect and antioxidant capacity, and protects the structure and function of the intestinal tract. The application effectively solves the problems of poor stability and insufficient targeting in oral delivery of quercetin, and provides a green and efficient solution for colon-targeted delivery of polyphenol functional factors.

Methods for inserting a targeting moiety (such as an antigen-binding protein moiety, a fragment antigen-binding moiety, or the like) into a lipid nanoparticle and compositions resulting from such methods are provided. The methods generally utilize a reaction that forms targeted nanoparticles by combining a targeting moiety and a lipid nanoparticle. A quenching operation is then provided by cooling the reaction to a temperature that stops the insertion of the targeting moiety. The reaction substantially preserves the integrity of a nucleic acid cargo (such as an mRNA encoding a VHH binding molecule).

The application provides an ultrasmall size CeO2-Gd@BSA-cRGD targeted nanoparticle and a green preparation method thereof, and belongs to the field of nanomedicine. The preparation method comprises the following steps: under stirring, a cerium salt solution is added into a BSA-cRGD solution prepared in step (1), then a gadolinium salt solution is added, after stirring, a mixed solution is obtained; under stirring, a sodium hydroxide solution is added into the mixed solution, the pH of the system is adjusted to 11-12, stirring reaction is carried out, dialysis purification is carried out, and the CeO2-Gd@BSA-cRGD targeted nanoparticle is obtained. The method is simple, easy to control, green, mild and suitable for large-scale production; the nanoparticle size is ultrasmall and the dispersity is good; the targeted tumor tissue permeability is better; the kidney metabolism can be carried out, and the side effects are reduced.

The invention relates to a preparation method of an FZD1 active targeting nanoparticle. The preparation method comprises the following steps: S1, synthesizing DSPE-PEG2000-UM206 by using DSPE-PEG2000-COOH and a polypeptide UM206; and S2, synthesizing the FZD1 active targeting nanoparticles by using the polylactic acid-glycolic acid copolymer and the DSPE-PEG2000-UM206 (Distearoyl Phosphate Polyethylene Glycol 2000-UM206). Due to the adoption of the technical scheme, the FZD1 active targeting nanoparticles prepared by the preparation method of the FZD1 active targeting nanoparticles can promote the phagocytosis of osteosarcoma cells to the PLGA nanoparticles; the phagocytosis efficiency of osteosarcoma dry-like cells (OCSCs for short in subsequent description) on the FZD1 active targeting nanoparticles prepared by the preparation method of the FZD1 active targeting nanoparticles is high.

The invention relates to the technical field of preparation of targeted nanoparticles and gel materials, and discloses a nanoparticle hydrogel spray containing targeted NRP1 as well as a preparation method and application of the nanoparticle hydrogel spray, the nanoparticle is of a core-shell structure, polydopamine is taken as a carrier material, the core is obtained by connecting Tat-C-RP7 and PDA-NPs through a Schiff base reaction, and the shell is ZIF8. The preparation method of the hydrogel spray comprises the following steps: step 1, synthesis of nanoparticles: synthesizing PDA-TCR7-ZIF8-NPs by a three-step method; and step 2, preparation of hydrogel spray: resuspending the PDA-TCR7-ZIF8-NPs in a beta-sodium glycerophosphate solution, and uniformly mixing and stirring the PDA-TCR7-ZIF8-CS (at) GP hydrogel solution with a chitosan solution under an ice-water bath condition, so as to obtain a PDA-TCR7-ZIF8-CS (at) GP hydrogel solution. The nano hydrogel can accurately target NRP1, and can reduce the scar area by 60% and the skin thickness from 1.8 mm to 1.1 mm in animal experiments aiming at the key vascular abnormality generation and fibrosis mechanism for scar formation, and can improve the scar color and flatness.

The application discloses a preparation method and application of a multi-morphology fasudil targeted nanomedicine system for diabetes osteoarthritis, and belongs to the technical field of medicines, and comprises the following steps: 1) weighing free fasudil, dissolving the free fasudil in an organic phase solvent, fully stirring and dissolving, and preparing fasudil organic drug stock solution, which is marked as Fas; 2) slowly adding an acid solution to the Fas; the application takes free fasudil as an active drug, constructs Fas@PB-Sr 2+ The composite nano intermediate is subjected to CAP polypeptide targeted modification to obtain multi-morphology targeted nanoparticles. Through a low-temperature hydrothermal process, drug thermal degradation is avoided, square, rod, spherical and porous mesoporous composite morphologies are generated by regulating raw material ratio and reaction conditions, and cartilage specificity enrichment is realized in combination with the CAP polypeptide, multiple pathological damages such as cartilage wear, synovial inflammation and abnormal vascular hyperplasia of diabetes osteoarthritis are improved, joint local drug residence time is prolonged, and systemic drug toxic side effects are reduced.

The invention discloses targeted nanoparticles for breast cancer treatment as well as preparation and application thereof, and belongs to the technical field of biological medicines. The invention provides a three-mode synergistic anti-tumor strategy of chemotherapy-photothermal therapy-Fenton-like reaction mediated ferroptosis for the first time. The targeting nanoparticles are efficiently enriched in 4T1 breast cancer cells by virtue of the dual-targeting effect of the ET peptide and the EPR; in combination with photothermal therapy, the chemotherapy effect of PTX is remarkably enhanced, and proliferation, invasion and metastasis of tumor cells are effectively inhibited. Proteomics analysis finds that differential proteins are mainly enriched in motor proteins and ferroptosis pathways after intervention of the targeted nanoparticles, and a core mechanism of collaborative treatment is disclosed. Molecular docking proves that PTX and a key target protein receptor have specific interaction, and the multi-mode synergistic strategy provides an innovative technical platform for overcoming the limitation of traditional chemotherapy and developing an efficient and low-toxicity breast cancer treatment scheme.