79 results about "Targeted nanoparticles" patented technology

The invention belongs to the field of biological medicine and ultrasonic medicine, and provides a barium titanate (BTO) piezoelectric material-based ultrasonic responsive targeting nano system (BTO at BAL), which is used for matrix microenvironment remodeling and immunopotentiation of compact tumors such as pancreatic cancer and the like. According to the nanoparticles, oleic acid modified BTO is taken as a core, ROS responsive prodrug molecules containing a small molecule PD-L1 inhibitor BMS1166 and a nitric oxide donor (Arg) 9 are loaded on the surface, and specific targeting on pancreatic cancer cells is realized by combining a targeting peptide LFC131. Under ultrasonic excitation, BTO generates reactive oxygen species (ROS), on one hand, (Arg) 9 is oxidized to release NO, tumor matrix is degraded, tumor hardness is reduced, drug delivery efficiency is improved, and meanwhile, ROS and NO synergistically enhance tumor immunogenicity; on the other hand, the ROS breaks a thioketal bond to release BMS1166, PD-L1 expression is down-regulated, and immunosuppression is reversed. According to the present invention, the significant tumor inhibition and immune activation effects are represented in the animal model, and the clinical transformation potential is provided.

The invention discloses a block magnesium-based composite material capable of quantitatively regulating and controlling the content of nano-particles and a manufacturing method of the block magnesium-based composite material, and belongs to the technical field of metal-based composite material preparation. The method comprises the following steps: providing a magnesium alloy substrate and a cover plate, determining the sectional dimension of a groove of the cover plate according to the volume fraction of target nanoparticles, processing the groove, then filling the groove with the nanoparticles, assembling the cover plate on the substrate in an inverted buckling manner to form a to-be-processed assembly, performing multi-pass stirring friction processing to form a first composite material layer, and stacking the first composite material layer as a new substrate layer by layer to form a second composite material layer; and finally, the target block magnesium-based composite material is prepared. Through the correlation design of the groove size and the target volume fraction, accurate control and uniform dispersion of the nano-particle content are achieved, the problems that a traditional method is high in defect rate, limited in size and the like are solved, the strength and plasticity of the prepared material are remarkably improved, the process is green and environmentally friendly, and the method is suitable for mass production of large-size components and can be popularized to various light metal matrixes; the application prospect is wide.

The application relates to a T cell-targeting nanoparticle based on a nucleic acid aptamer and a preparation method and application thereof, in particular to a T cell-targeting lipid aptamer, wherein the lipid aptamer comprises a hydrophobic molecule and a nucleic acid aptamer, the nucleic acid aptamer comprises at least one nucleic acid aptamer for targeting a T cell surface molecule, and the molar ratio of the hydrophobic molecule and the nucleic acid aptamer is 5000:1-1:10. The lipid aptamer or the nanoparticle formed by self-assembly technology of the lipid aptamer can realize cell 'pointing' gene modification and in-vivo gene reprogramming of cells.

Compositions and methods are provided for modifying the contents of exosomes released by target cells. In one embodiment cell targeted nanoparticles are used to deliver interference RNAs to the target cells which alter the packaging of exosomes released by the target cells.

The application belongs to the technical field of targeted drugs, and discloses a carrier, a targeted nanoparticle and a preparation method and application thereof. The carrier disclosed by the application comprises darpin grafted with a dye containing a terminal carboxyl group. The targeted nanoparticle comprises the carrier and a CO releasing molecule, and the carrier coats the CO releasing molecule to form a core-shell structure. The targeted nanoparticle is based on darpin, the grafting of the dye containing the terminal carboxyl group is performed to construct the carrier, and then the carrier coats the CO releasing molecule, wherein the carrier has a targeting effect, and the photothermal effect of the carrier can realize the NIR laser controlled CO releasing or free radical stimulation, and can also target the controllable CO releasing, so that the double response releasing of exogenous CO and endogenous CO is realized, and the application has a wide application prospect.

The application belongs to the field of medical materials, and particularly relates to a metal core enhanced double-barrier piezoelectric nano system and a preparation method and application thereof. The preparation method comprises the following steps: first, a core-shell structure of'metal@mesoporous titanium dioxide@mesoporous barium titanate' is prepared through a step-by-step reaction, and then the target nanoparticles are obtained through lipid wrapping. The system innovatively constructs a double-barrier structure, can self-trigger active oxygen under a physiological pressure of 4 mmHg, realizes endogenous pressure self-driven treatment of malignant ascites, has the advantages of low pressure, high catalytic efficiency, long-term structural stability and good biocompatibility, solves the problems that existing treatment methods are highly invasive and depend on exogenous stimulation, and provides a new safe and efficient technical scheme for the treatment of malignant ascites.

This disclosure relates to compositions and methods for treating lung disorders, including, for example, pulmonary fibrosis, and other fibrotic disorders. Thioredoxin domain-containing 5 (TXNDC5) is significantly increased in fibrotic lungs from human PF patients. Therefore, a targeted nanoparticle comprising an inhibitor of TXNDC5 was developed, which may have potential to treat pulmonary fibrosis.

The invention discloses preparation and application of algal polysaccharide-based co-drug-loading tumor targeting nanoparticles. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is a self-assembled targeting nano particle co-constructed by biotin modified algal polysaccharide sulfate and arginine modified chitosan. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is an algal polysaccharide-based co-drug-loaded nano particle. The algal polysaccharide-based nanoparticles encapsulate anti-tumor hydrophilic drugs and hydrophobic drugs to realize targeted co-delivery of the drugs in solid tumor tissues, self-assembly is generated through electrostatic interaction of polysaccharide molecules to drive the encapsulated drugs to enter inner cores of the nanoparticles, and meanwhile biotin molecules are mainly distributed in shell layers of the nanoparticles. According to the algal polysaccharide-based co-drug-loading nano particle, the targeting property to solid tumor tissues is enhanced, the defect that a conventional drug-loading system can only encapsulate a single hydrophilic or hydrophobic drug is innovatively overcome, efficient co-delivery of hydrophilic and hydrophobic drugs with different attributes is realized, and proliferation and migration of tumors can be remarkably inhibited.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The present application relates to the technical field of targeted nanoparticles, and particularly relates to a mitochondria-targeting autophagy-inhibiting polymer and a preparation method thereof, a nano delivery particle and a preparation method and application thereof. A high molecule TPP-PEG-SH (TPS) containing a triphenylphosphonium group (triphenylphosphonium, TPP) is coupled with a mitochondria autophagy inhibitor Mdivi-1 through a disulfide bond to construct a polymer TPSM with mitochondria targeting ability and GSH responsiveness, and further co-assembled with a photosensitizer IT-4Cl to form a nano delivery system TPSM@IT-4Cl. The nano delivery system has mitochondria targeting ability, promotes continuous accumulation of oxidative stress at a tumor site, and can realize responsive drug release in a high-concentration GSH tumor microenvironment.

The invention relates to the technical field of biological medicine preparations, in particular to application of targeted nanoparticles in treatment of osteoporosis. The targeted nano-particles CL-NPs (at) NEs are developed, CL can stimulate lipolysis of bone marrow adipocytes, osteogenic differentiation is promoted, and therefore the bone marrow microenvironment is improved; meanwhile, autologous neutrophil is used as a carrier to transport CL-NPs, bone marrow fat cells beta3AR are accurately targeted, and the osteoporosis microenvironment can be further improved. Experiments prove that CL-NPs (at) NEs stimulate a bone marrow fat cell beta3 adrenoceptor in a targeted manner, can effectively improve OP, has safety and has the potential to become a novel medicine for osteoporosis.

The invention relates to the field of drug delivery, in particular to a prediction method and device for nanoparticle assembly and a program product. Comprising the following steps: acquiring three-dimensional compound molecules of a target compound and a target nanoparticle size; serializing the topological structure of the three-dimensional compound molecule to obtain a topological sequence; obtaining a three-dimensional coordinate of each atom according to the sequence of the atoms in the topological structure to obtain a molecular conformation sequence; discretizing the size of the target nanoparticles to obtain a size sequence; integrating the topological sequence and the size sequence to obtain a first topological sequence, and integrating the molecular conformation sequence and the size sequence to obtain a first conformation sequence; and outputting the first topological sequence and the first conformation sequence to a neural network for feature extraction, and then performing prediction to obtain metal ions, organic compound additives and corresponding three-dimensional conformations of nanoparticles with specified sizes. The method has good application value.

The present invention provides: a method for preparing tissue-targeting nanoparticles, the method comprising a step for generating phospholipid nanoparticles, a step for generating mesenchymal stem cell-derived cell membrane nanoparticles, and a step for fusing the generated phospholipid nanoparticles and cell membrane nanoparticles to generate tissue-targeting nanoparticles; and tissue-targeting nanoparticles which are prepared by the method and comprise bisphosphonate-containing phospholipid nanoparticles and mesenchymal stem cell-derived cell membrane nanoparticles.

This invention discloses a nanoparticle targeting bone tissue macrophages, its preparation method, and its applications, belonging to the field of biomedical technology. This invention employs a dual targeting mechanism, modifying the nanoparticle surface with bone-like peptide sequences and mannose-modified ligands, enabling the nanoparticles to specifically recognize macrophages in the bone tissue environment. The bone tissue macrophage-targeting nanoparticle system prepared by this invention can significantly improve targeting efficiency, anti-inflammatory effects, and tissue repair effects, while reducing systemic side effects, providing a new treatment approach for bone injury repair and showing promising clinical application prospects.

Provided herein are compositions, methods, and particles that include a peptide that targets placental chondroitin sulfate A (placental CSA), wherein the amino acid sequence of the polypeptide is as set forth in SEQ ID NO: 1 (EDVKDINFDTKEKFLAGLIVSFHEGKC).

The invention provides cardiac targeting nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The heart targeting nanoparticle provided by the invention contains the mesoporous silica nanoparticle, the myocardial targeting peptide and the SNX7 inhibitor, and can significantly improve the enrichment efficiency of a therapeutic drug at a heart part and reduce the exposure of the drug in non-target tissues, so that the heart function is effectively improved and the potential toxic and side effects are reduced; the nanoparticle system has excellent biocompatibility and stability, provides a new solution for precise treatment of heart failure, has wide clinical application prospects, and is expected to promote progress in the field of cardiovascular disease treatment.

The invention discloses a nanoparticle capturing method and related equipment, and the method comprises the following steps: uniformly covering a hydrogel solution on a sample liquid drop containing target nanoparticles on a glass slide to form a standard observation system; the hydrogel solution is temperature sensitive type hydrogel; determining a local area where the target nanoparticles are located in a standard observation system; starting a laser, controlling the laser to irradiate the local area, and regulating and controlling the laser power density of the laser, so that the temperature of the local area reaches and is maintained at the phase change temperature of the hydrogel solution; and adjusting laser parameters of the laser to generate stable hydrogel at the position of the target nanoparticle. According to the embodiment of the invention, the nano-particles can be captured by utilizing the characteristics of the temperature-sensitive hydrogel and combining a simple photo-thermal device, the influence caused by the limitation of capturing environment conditions or capturing sample properties is reduced, and the method can be widely applied to the technical field of nano-particle capturing.

This application discloses a tumor-targeting nanoparticle, its preparation method, and its application, belonging to the field of tumor drug technology. The tumor-targeting nanoparticle includes a drug and a delivery carrier loading the drug, wherein the drug is atorvaquinone. The delivery carrier is prepared by coupling an intermediate product with Sulfo-SMCC-BSA. The intermediate product is obtained by reducing a thiol-modified nucleic acid aptamer, wherein the nucleic acid aptamer is EGFR aptamer. This application utilizes a EGFR-targeting nucleic acid aptamer coupled with bovine serum albumin (BSA) to encapsulate atorvaquinone, successfully constructing a novel atorvaquinone nanomedicine. This improves the targeting ability of atorvaquinone and increases its water solubility, providing a new approach for the treatment of esophageal cancer with atorvaquinone nanomedicines.

The invention discloses preparation and application of macrophage targeting nanoparticles entrapped with a cell burial function accelerant. The medicine for enhancing the intercellular function of the macrophages is a liver X receptor stimulant, and the liver X receptor stimulant comprises at least one of T0901317, GW3965, Ouabagenin and LXR-623. The surface of the targeting nanoparticle is coated with a macrophage cell membrane, and meanwhile, a urokinase type plasminogen activator receptor (uPAR) targeting ligand is modified. The nanoparticle can be efficiently accumulated at an inflammatory focus part by utilizing a macrophage cell membrane, and the modified targeting ligand is used for targeting the macrophage with damaged cell burial function, so that the cell burial function of the macrophage is enhanced, the polarization of the macrophage to an anti-inflammatory phenotype is promoted, the release of anti-inflammatory and proinflammatory cytokines is regulated, and the anti-inflammatory effect of the nanoparticle is improved. And finally, the inflammatory microenvironment of a focus part is regulated and controlled, so that a good effect of treating autoimmune diseases is achieved.

The development of a new, targeted drug delivery paradigm coupled to improved PI3K inhibitors (e.g., PI3Kα inhibitors) represents a significant advance in cancer therapy. Provided herein are compounds, such as compounds of Formula (I) and (II), and pharmaceutically acceptable salts, hydrates, solvates, polymorphs, co-crystals, tautomers, stereoisomers, isotopically labeled derivatives, and prodrugs thereof. The compounds provided herein are PI3K (e.g., PI3Kα) inhibitors and are therefore useful for the treatment and / or prevention of various diseases (e.g., proliferative diseases such as cancer). Also provided herein are nanoparticles and nanogels (e.g., P-selectin targeting nanoparticles) comprising PI3K inhibitors, such a compound described herein. In certain embodiments, a nanoparticle or nanogel described herein encapsulates a compound described herein for targeting delivery to cancer cells or tumors.

The application discloses application of a silk fibroin nanoparticle hydrogel in treatment of pressure sore ulcers and relates to the technical field of biochemistry.The core points of the technical solution include the following steps: S1, preparation of a silk fibroin solution;S2, preparation of nanoparticles;S3, production and preparation of milk fat globule epidermal growth factor VIII (MFG-E8) in exosomes;S4, purification of MFG-E8 protein;S5, preparation of silk fibroin nanoparticles coated with MFG-E8;S6, preparation of MFG-E8-silk fibroin nanoparticles crosslinked with NGR peptides; and S7, preparation of a collagen / silk fibroin hydrogel.The silk fibroin nanoparticle hydrogel is a non-invasive treatment method and is more easily accepted by patients in clinical application.In addition, the targeted nanoparticle treatment method for pressure sore sites can significantly improve the treatment effect of patients.

This invention discloses a plant exosome-liposome composite targeted nanoparticle drug delivery system, its preparation method, and its applications. The nanoparticle drug delivery system comprises plant exosomes and liposomes, as well as a targeting polypeptide linked to the surface of the liposomes and an antioxidant drug encapsulated within the liposomes. The plant exosome-liposome composite targeted nanoparticle drug delivery system prepared by this invention can penetrate the blood-brain barrier, possesses the ability to actively target nerve cells, exhibits good drug carrier stability, and is not easily degraded by macrophages or the reticuloendothelial system. Combining the dual therapeutic advantages of the active substances carried by plant exosomes and the antioxidant drugs, it achieves both the regulation of autophagy in nerve cells and the inhibition of neuroinflammation, realizing multi-target therapy and providing a new approach and drug for the efficient treatment of Parkinson's disease.

The invention provides a lysozyme-based quercetin colon-targeted nanoparticle as well as a preparation method and application of the lysozyme-based quercetin colon-targeted nanoparticle. According to the invention, a quercetin-Fe < 2 + > metal polyphenol network (MPN) nano core is constructed by a solvent exchange method, and then the quercetin-Fe < 2 + > metal polyphenol network (MPN) nano core, positively charged egg white lysozyme and negatively charged pectin are subjected to electrostatic self-assembly in sequence to form the nano particle with a triple coating structure. The encapsulation rate of quercetin in the nano-particles ranges from 77% to 98%, the drug loading rate ranges from 13% to 32.2%, and the nano-particles have excellent gastrointestinal stability and colon targeting. High-fat model animal experiment results show that the nanoparticles have good lipid-lowering effect and oxidation resistance, and can protect intestinal structures and functions. The problems of poor stability and insufficient targeting in oral delivery of quercetin are effectively solved, and a green and efficient solution is provided for colon targeting delivery of polyphenol functional factors.

This invention relates to the field of granule preparation technology, and discloses a Coptis chinensis preparation for treating gastrointestinal damp-heat stagnation and its preparation method. First, the preparation components, including total alkaloids of Coptis chinensis, volatile oil of Aucklandia lappa, dry extract powder of excipient medicinal materials, chitosan, and pectin, are determined according to specific weight ratios. For different components, supercritical CO2 extraction, dynamic cyclic extraction combined with near-infrared online monitoring technology, and standardized water decoction process are used to accurately extract and prepare the preparation. Then, a chitosan-pectin nano-targeted delivery system is constructed, and colon-targeted nanoparticles are obtained by modifying colonic epithelial cell-targeting peptides. These nanoparticles are then obtained by vacuum freeze-drying and fluidized bed microsphere granulation. Simultaneously, the volatile oil of Aucklandia lappa is treated with β-cyclodextrin inclusion complex. Finally, the nanoparticles are mixed with the volatile oil inclusion complex, dry extract powder of excipient medicinal materials, enteric-coated excipients, and fillers to prepare a thermosensitive bio-ink. A thermosensitive gel framework preparation is then obtained using semi-solid extrusion 3D printing technology.

Provided herein are cancer-targeted nanoparticles for the treatment or prevention of cancer.

The invention relates to the technical field of wash supplies, in particular to Chinese herbal medicine hair loss preventing and hair blackening shampoo containing hair follicle targeted delivery nanoparticles and a preparation method of the Chinese herbal medicine hair loss preventing and hair blackening shampoo. The invention discloses a skin-care lotion, which is prepared from the following components by weight: 10 to 15 g of cacumen biotae extract, 8 to 12 g of radix polygoni multiflori extract, 6 to 10 g of rhizoma zingiberis recens extract, 5 to 8 g of semen sesami nigrum extract, 3 to 5 g of chitosan, 1 to 3 g of hyaluronic acid, 0.5 to 1 g of targeting peptide, 8 to 12 g of sodium laureth sulfate, 5 to 8 g of cocamidopropyl betaine, 3 to 5 g of glycerol, 1 to 2 g of sodium chloride, 0.5 to 1 g of citric acid, 0.2 to 0.5 g of essence and 1000 ml of deionized water. Various Chinese herbal medicine extracts such as cacumen biotae, radix polygoni multiflori, fresh ginger and black sesame are carefully selected, the components have a synergistic effect, hair follicles can be fundamentally nourished, the scalp environment can be adjusted, a hair follicle targeting nanoparticle carrier is innovatively introduced, the effective components of the Chinese herbal medicines can be accurately delivered to the hair follicles by utilizing chitosan, hyaluronic acid and targeting peptide, and the effect of treating the scalp is achieved. The absorption efficiency of the active ingredients is greatly improved, so that the functional ingredients can fully play roles, and the hair loss preventing and hair blackening effects are remarkably enhanced.

The invention relates to the technical field of medicines, and particularly discloses a preparation method and application of cascaded targeted anti-inflammatory nanoparticle responsive hydrogel, and the technical key points are as follows: the method comprises the following steps: S1, synthesizing cascaded targeted nanoparticles MPPT (Maximum Power Point Tracking); s2, synthesis of an active oxygen responsive hydrogel component TSPBA; and S3, preparation of the cascaded targeted nano particle hydrogel MPPT (at) TSPBA-PVA (Polyvinyl Alcohol). Aiming at the characteristic of dynamic unbalance of inflammatory macrophages and microenvironment ROS (reactive oxygen species) of diabetic periodontitis, the MPPT-TSPBA-PVA hydrogel platform with active oxygen responsiveness and macrophage mitochondrial cascade targeting performance is designed: (1) the MPPT-TSPBA-PVA hydrogel platform is endowed with accurate macrophage and mitochondrial targeting performance by utilizing the targeting effect of phagocytic peptide and MitoQ; (2) the platform is endowed with response release efficiency by using the ROS responsiveness of the TSPBA-PVA hydrogel, and (3) the purposes of efficiently removing ROS and reducing local inflammatory response are finally achieved.

The invention relates to a supercritical anti-solvent-electrostatic spray coupling system which comprises a supercritical anti-solvent generation module, a material mixing module and an electrostatic atomization module which are sequentially connected through a connecting pipeline, and a time-space synchronization control module which is in communication connection with the supercritical anti-solvent generation module and the electrostatic atomization module. The space-time synchronous control module cooperatively controls the oscillating electric field and the pressure of the supercritical anti-solvent, so that the electric field jet breaking of the material is realized by the electric field force, and the absolute value of the difference value between the Taylor cone forming time and the supercritical anti-solvent diffusion time is controlled within a set range, so that the supercritical anti-solvent permeates into liquid drops within a millisecond-level time scale; the invention discloses a preparation method of brain-targeted nano-particles, which comprises the following steps: dissolving a solvent in a solvent to trigger a sudden drop effect of polarity of the solvent, so that solute is instantaneously crystallized, and finally a nano-suspension with a particle size of less than 200nm and uniform particle size distribution (PDilt: 0.2) is formed, is suitable for preparing brain-targeted nano-particles with high drug loading capacity and small particle size, and can effectively cross a blood-brain barrier (BBB) to realize continuous and green production.

This invention discloses a targeted nanoparticle for calcium overload therapy combined with photothermal therapy, specifically involving a lipid-modified cRGD-targeting, MMP-responsive, and pH-sensitive calcium carbonate nanoparticle loaded with indocyanine green (ICG). This invention uses pH-sensitive calcium carbonate, which can decompose and exfoliate within lysosomes, as the matrix material. It leverages cRGD's specific targeting of tumor cells, the tumor microenvironment-specific high expression of matrix metalloproteinases (MMPs) to induce the release of DPPA-1 within the tumor microenvironment, and the low pH characteristic within lysosomes to achieve a combined anti-tumor effect of phototherapy and immunotherapy.

This invention provides nanoparticles with plaque-targeting function, their preparation method, and their application in the preparation of anti-atherosclerotic drugs, belonging to the field of composite nanomaterials technology. The plaque-targeting nanoparticles provided by this invention comprise a core and a shell. The core is ZIF-8 nanoparticles loaded with neoindocyanine green, and the shell is arginine-modified polydopamine; the surface of the shell is modified with hyaluronic acid. The plaque-targeting nanoparticles provided by this invention can regulate lipid levels by simultaneously intervening in multiple pathways, providing anti-inflammatory effects, protecting damaged endothelial cells, and enabling low-temperature (42-45℃) photothermal therapy combined with anti-nlrp3 therapy. This treats atherosclerosis by regulating PCSK9 pathway-induced lipid accumulation and repairing damaged endothelial cells.