106 results about "Targeted nanoparticles" patented technology

The invention provides a dual-targeting carrier material, a preparation method thereof and a preparation method of oleanolic acid nanoparticles wrapped by the dual-targeting carrier material, and belongs to the technical field of medicines. The oleanolic acid targeting nanoparticles are prepared by taking a dual-targeting high-molecular copolymer as a carrier material and adopting an emulsification-solvent evaporation method, so that the oleanolic acid can be protected from being quickly degraded, and the in-vivo circulation time of the oleanolic acid can be prolonged. Meanwhile, targeted delivery of oleanolic acid is realized through a targeted group, the concentration of the drug in tumor tissues is improved, and toxic and side effects on normal tissues are reduced. An MTT method is adopted, the inhibition effect of the oleanolic acid nanoparticles on cell growth is researched, and the result shows that the oleanolic acid targeting nanoparticles have a stronger effect of inhibiting tumor cell proliferation compared with an oleanolic acid raw material medicine.

The embodiment of the invention provides breast cancer targeting nanoparticles as well as a preparation method and application thereof. According to the embodiment of the invention, the breast cancer targeting nanoparticles are synthesized by preparing the pH sensitive polymer, carrying out co-coupling modification on the pH response polymer and the folic acid albumin nano-carrier and utilizing a controlled desolvation method; the breast cancer targeting nanoparticle is combined with a nano preparation passive targeting strategy, a tumor microenvironment stimulation response strategy and a folic acid active targeting strategy, the effects of an EPR effect, PEG shedding, charge overturning, folic acid ligand-receptor combination and the like are utilized, precise targeting and specific response of tumors are achieved, and targeted accumulation of drugs in a tumor microenvironment is comprehensively improved. Therefore, the breast cancer targeting nanoparticles prepared by the embodiment have the advantages of high targeting property, drug controlled release, improvement of solubility and absorptivity of indissolvable drugs and the like, so that the curative effect of the drugs can be enhanced, the toxic and side effects can be reduced, and the breast cancer targeting nanoparticles have wide application prospects in the field of anti-tumor treatment.

The invention provides a combined use of a hyaluronic acid-bilirubin conjugate and honokiol in preparation of a medicine for treating osteoarthritis. The invention provides a honokiol-loaded nanoparticle, which is characterized in that a hyaluronic acid-bilirubin conjugate and honokiol are used as raw materials to prepare a nanoparticle HKL-PNPs, and then the nanoparticle HKL-PNPs is modified by a mitochondrial targeting peptide to obtain the honokiol-loaded nanoparticle HKL-MPNPs. The invention also provides a bionic nanoparticle for treating bone joints. The bionic nanoparticle is prepared by wrapping the honokiol-loaded nanoparticle with an engineered cartilage cell membrane (ECM). According to the invention, cartilage cells with high expression of IL-1R2 are obtained through a gene editing method for the first time, and mitochondrial targeted nanoparticle cores loaded with Sirtuin-3 agonists are coated by cell membranes of the cells, so that the bionic nano preparation is obtained and is used for treating OA. The prepared bionic nano preparation not only can neutralize IL-1beta mediated inflammatory reaction, but also can recover Sirtuin-3 mediated mitochondrial function imbalance, so that the result of synergistic treatment of OA is achieved.

The invention belongs to the field of biological medicine and ultrasonic medicine, and provides a barium titanate (BTO) piezoelectric material-based ultrasonic responsive targeting nano system (BTO at BAL), which is used for matrix microenvironment remodeling and immunopotentiation of compact tumors such as pancreatic cancer and the like. According to the nanoparticles, oleic acid modified BTO is taken as a core, ROS responsive prodrug molecules containing a small molecule PD-L1 inhibitor BMS1166 and a nitric oxide donor (Arg) 9 are loaded on the surface, and specific targeting on pancreatic cancer cells is realized by combining a targeting peptide LFC131. Under ultrasonic excitation, BTO generates reactive oxygen species (ROS), on one hand, (Arg) 9 is oxidized to release NO, tumor matrix is degraded, tumor hardness is reduced, drug delivery efficiency is improved, and meanwhile, ROS and NO synergistically enhance tumor immunogenicity; on the other hand, the ROS breaks a thioketal bond to release BMS1166, PD-L1 expression is down-regulated, and immunosuppression is reversed. According to the present invention, the significant tumor inhibition and immune activation effects are represented in the animal model, and the clinical transformation potential is provided.

The present invention relates to the field of targeted therapy technology, and particularly, to a carrier, a targeted nanoparticle, a method for preparing same, and use thereof. The carrier disclosed in the present invention comprises tocilizumab grafted with a terminal carboxyl group-containing dye. The targeted nanoparticle comprises a carrier and a CO-release molecule. The carrier encapsulates the CO-release molecule to form a core-shell structure. In the targeted nanoparticle based on tocilizumab, the carrier is constructed by grafting the terminal carboxyl group-containing dye, and the carrier encapsulates the CO-release molecule. The carrier possesses a targeting effect, and the photothermal effect of the carrier can achieve the regulated CO release by NIR laser or targeted and controllable CO release under free radical stimulation, thereby achieving exogenous / endogenous dual-response release of CO. The present invention features good application prospects.

The invention discloses a block magnesium-based composite material capable of quantitatively regulating and controlling the content of nano-particles and a manufacturing method of the block magnesium-based composite material, and belongs to the technical field of metal-based composite material preparation. The method comprises the following steps: providing a magnesium alloy substrate and a cover plate, determining the sectional dimension of a groove of the cover plate according to the volume fraction of target nanoparticles, processing the groove, then filling the groove with the nanoparticles, assembling the cover plate on the substrate in an inverted buckling manner to form a to-be-processed assembly, performing multi-pass stirring friction processing to form a first composite material layer, and stacking the first composite material layer as a new substrate layer by layer to form a second composite material layer; and finally, the target block magnesium-based composite material is prepared. Through the correlation design of the groove size and the target volume fraction, accurate control and uniform dispersion of the nano-particle content are achieved, the problems that a traditional method is high in defect rate, limited in size and the like are solved, the strength and plasticity of the prepared material are remarkably improved, the process is green and environmentally friendly, and the method is suitable for mass production of large-size components and can be popularized to various light metal matrixes; the application prospect is wide.

The application relates to a T cell-targeting nanoparticle based on a nucleic acid aptamer and a preparation method and application thereof, in particular to a T cell-targeting lipid aptamer, wherein the lipid aptamer comprises a hydrophobic molecule and a nucleic acid aptamer, the nucleic acid aptamer comprises at least one nucleic acid aptamer for targeting a T cell surface molecule, and the molar ratio of the hydrophobic molecule and the nucleic acid aptamer is 5000:1-1:10. The lipid aptamer or the nanoparticle formed by self-assembly technology of the lipid aptamer can realize cell 'pointing' gene modification and in-vivo gene reprogramming of cells.

The present invention belongs to the technical field of anti-cancer drugs, and particularly relates to a cerium vanadate targeted nanoparticle, a preparation method thereof and an application. The cerium vanadate targeted nanoparticle provided by the present invention comprises a drug core, a protective shell layer wrapping the drug core; and a targeting material modified on the outer surface of the protective shell; the drug core comprises CeVO<subgt;4< / subgt> nanoparticles. The cerium vanadate targeted nanoparticle provided by the present invention uses CeVO<subgt;4 nanoparticles as a drug carrier. Since the CeVO<subgt;4 nanoparticles have cytochrome c oxidase-like catalytic activity and cooperate with endogenously overexpressed cytochrome c in cancer cells to reduce and consume O<subgt;2, the oxygen concentration in the tumor microenvironment is effectively reduced. The cerium vanadate targeted nanoparticle provided by the present invention enhances the targeting of the nanoparticle to cancer cells, increases the probability of the nanoparticle entering cancer cells, can activate the drug to play a role in a hypoxic environment, and effectively kills tumor cells.

The invention discloses a targeting nanoscale particle, a targeting cell as well as a preparation method and application of the targeting nanoscale particle and the targeting cell. Wherein the outer surface of the targeting cell is combined with a targeting nano-scale particle, the targeting nano-scale particle is formed by mutually connecting a plurality of proteins through a first combination part, the targeting nano-scale particle further comprises a second combination part, and the targeting nano-scale particle is combined with the outer surface of the target cell through the second combination part. In an exemplary embodiment, the targeting nanoscale particles can promote interaction between the chimeric antigen receptor T cells and the leukemia cells by being simultaneously combined with the chimeric antigen receptor T cells and the leukemia cells, and then the chimeric antigen receptor T cells are promoted to recognize and kill the leukemia cells. In addition, a space is provided for loading of chemotherapeutic drugs by a protein internal cavity in the targeting nanoscale particles, and combined therapy of the chimeric antigen receptor T cells and other therapies is realized while the drugs are loaded.

Compositions and methods are provided for modifying the contents of exosomes released by target cells. In one embodiment cell targeted nanoparticles are used to deliver interference RNAs to the target cells which alter the packaging of exosomes released by the target cells.

The application belongs to the technical field of targeted drugs, and discloses a carrier, a targeted nanoparticle and a preparation method and application thereof. The carrier disclosed by the application comprises darpin grafted with a dye containing a terminal carboxyl group. The targeted nanoparticle comprises the carrier and a CO releasing molecule, and the carrier coats the CO releasing molecule to form a core-shell structure. The targeted nanoparticle is based on darpin, the grafting of the dye containing the terminal carboxyl group is performed to construct the carrier, and then the carrier coats the CO releasing molecule, wherein the carrier has a targeting effect, and the photothermal effect of the carrier can realize the NIR laser controlled CO releasing or free radical stimulation, and can also target the controllable CO releasing, so that the double response releasing of exogenous CO and endogenous CO is realized, and the application has a wide application prospect.

The application belongs to the field of medical materials, and particularly relates to a metal core enhanced double-barrier piezoelectric nano system and a preparation method and application thereof. The preparation method comprises the following steps: first, a core-shell structure of'metal@mesoporous titanium dioxide@mesoporous barium titanate' is prepared through a step-by-step reaction, and then the target nanoparticles are obtained through lipid wrapping. The system innovatively constructs a double-barrier structure, can self-trigger active oxygen under a physiological pressure of 4 mmHg, realizes endogenous pressure self-driven treatment of malignant ascites, has the advantages of low pressure, high catalytic efficiency, long-term structural stability and good biocompatibility, solves the problems that existing treatment methods are highly invasive and depend on exogenous stimulation, and provides a new safe and efficient technical scheme for the treatment of malignant ascites.

This disclosure relates to compositions and methods for treating lung disorders, including, for example, pulmonary fibrosis, and other fibrotic disorders. Thioredoxin domain-containing 5 (TXNDC5) is significantly increased in fibrotic lungs from human PF patients. Therefore, a targeted nanoparticle comprising an inhibitor of TXNDC5 was developed, which may have potential to treat pulmonary fibrosis.

The invention relates to molecular biology and biomedicine technologies, and aims to provide a preparation method and application of an efficient targeting swine fever nano vaccine. The invention provides an APC cell targeted hog cholera virus self-assembled protein nanoparticle, which is obtained by carrying out fusion connection expression on a recombinant plasmid of an APC cell targeted nanoparticle mi3-PTC with a sequence as shown in SEQ ID NO.3 and a hog cholera virus antigen with a sequence as shown in SEQ ID NO.5-12, and further mixing with pharmaceutically acceptable auxiliary materials to prepare a vaccine product for preventing and / or treating hog cholera virus infection. The APC cell targeting polypeptide can be displayed on the surface of the nanoparticle mi3, and the nanoparticle is directionally carried to the APC cell expressing CD163, so that the internalization, storage and distribution capabilities of the nanoparticle are remarkably improved in vivo and in vitro, and the vaccine can remarkably improve the immune response to the swine fever antigen.

The invention discloses preparation and application of algal polysaccharide-based co-drug-loading tumor targeting nanoparticles. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is a self-assembled targeting nano particle co-constructed by biotin modified algal polysaccharide sulfate and arginine modified chitosan. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is an algal polysaccharide-based co-drug-loaded nano particle. The algal polysaccharide-based nanoparticles encapsulate anti-tumor hydrophilic drugs and hydrophobic drugs to realize targeted co-delivery of the drugs in solid tumor tissues, self-assembly is generated through electrostatic interaction of polysaccharide molecules to drive the encapsulated drugs to enter inner cores of the nanoparticles, and meanwhile biotin molecules are mainly distributed in shell layers of the nanoparticles. According to the algal polysaccharide-based co-drug-loading nano particle, the targeting property to solid tumor tissues is enhanced, the defect that a conventional drug-loading system can only encapsulate a single hydrophilic or hydrophobic drug is innovatively overcome, efficient co-delivery of hydrophilic and hydrophobic drugs with different attributes is realized, and proliferation and migration of tumors can be remarkably inhibited.

The invention relates to the technical field of bioengineering, in particular to a targeting nanoparticle for treating acute respiratory distress syndrome as well as a preparation method and application of the targeting nanoparticle. Comprising the following steps: (1) combining MP peptide with DSPE-PEG2000-MAL through a maleimide-mercaptan reaction, dialyzing the obtained conjugate, washing, and freeze-drying, so as to obtain a DSPE-PEG2000-MP conjugate; and (2) mixing the conjugate, lipid, a pharmaceutical preparation and a solvent, removing the solvent to obtain a lipid film, hydrating the lipid film, and performing membrane extrusion to obtain the nanoparticles. The invention not only provides a novel efficient and safe ARDS treatment scheme, but also provides an innovative mode for the design of a nano-drug delivery system based on cell mediation. And due to the dual targeting capability, excellent anti-inflammatory efficiency and good biocompatibility, the nano-material has a wide prospect in clinical application.

The invention relates to the technical field of biological materials, in particular to bone targeting nanoparticles and a preparation method and application thereof.The preparation method comprises the steps that a curcumin organic solution, an icariin organic solution, an alendronate sodium solution and a DSPE-PEG-NHS solution are prepared; under the stirring condition, dropwise adding the icariin organic solution into the curcumin organic solution, continuously stirring for reaction after dropwise adding is completed, and self-assembling to form a curcumin-icariin intermediate; dispersing the curcumin-icariin intermediate in deionized water to obtain a dispersion liquid; and adding an alendronate sodium solution and a DSPE-PEG-NHS solution into the dispersion liquid, carrying out stirring reaction for a preset time, and carrying out dialysis treatment to obtain the bone-targeted nanoparticles. Various active ingredients with different action mechanisms can be integrated into a single nano-carrier, so that synergistic interaction and targeted delivery are realized.

The invention discloses a preparation method of lung and mitochondria dual-targeting nanoparticles, which utilizes a nanotechnology to efficiently load and accurately deliver an anti-inflammatory drug, reduces the frequency and dosage of medication, and reduces the side effects of the drug on patients. LSALTPSPSWLKYKAL is used for modifying lipidosome, and the LSA targeting peptide competitively inhibits aggregation of neutrophile granulocytes by being combined with a DPEP1 receptor, so that the effect of targeting the lung to deliver drugs can be achieved, aggregation of neutrophile granulocytes can be relieved, and lung injury can be accurately treated. The lipidosome core layer wraps the tannic acid cerium metal nanoparticles, not only has the mitochondrial targeting characteristic of tannic acid, but also can achieve the anti-oxidation effect through the change of the cerium metal valence, thereby reducing the generation of mitochondrial active oxygen, relieving the tissue oxidative stress and protecting mitochondria. The polyethylene glycol modified lipidosome can reduce the in-vivo clearance rate of the lipidosome, prolong the half-life period of the medicine and increase the compatibility with the body, and the surface modification utilizes the characteristics of diseases to carry out controllable release and increase the availability of the medicine. According to the invention, the progress of a drug delivery technology is promoted, and a new thought and a technical platform are provided for organelle targeted therapy.

The present application relates to the technical field of targeted nanoparticles, and particularly relates to a mitochondria-targeting autophagy-inhibiting polymer and a preparation method thereof, a nano delivery particle and a preparation method and application thereof. A high molecule TPP-PEG-SH (TPS) containing a triphenylphosphonium group (triphenylphosphonium, TPP) is coupled with a mitochondria autophagy inhibitor Mdivi-1 through a disulfide bond to construct a polymer TPSM with mitochondria targeting ability and GSH responsiveness, and further co-assembled with a photosensitizer IT-4Cl to form a nano delivery system TPSM@IT-4Cl. The nano delivery system has mitochondria targeting ability, promotes continuous accumulation of oxidative stress at a tumor site, and can realize responsive drug release in a high-concentration GSH tumor microenvironment.

The invention relates to the technical field of biological medicine preparations, in particular to application of targeted nanoparticles in treatment of osteoporosis. The targeted nano-particles CL-NPs (at) NEs are developed, CL can stimulate lipolysis of bone marrow adipocytes, osteogenic differentiation is promoted, and therefore the bone marrow microenvironment is improved; meanwhile, autologous neutrophil is used as a carrier to transport CL-NPs, bone marrow fat cells beta3AR are accurately targeted, and the osteoporosis microenvironment can be further improved. Experiments prove that CL-NPs (at) NEs stimulate a bone marrow fat cell beta3 adrenoceptor in a targeted manner, can effectively improve OP, has safety and has the potential to become a novel medicine for osteoporosis.

The invention relates to the field of drug delivery, in particular to a prediction method and device for nanoparticle assembly and a program product. Comprising the following steps: acquiring three-dimensional compound molecules of a target compound and a target nanoparticle size; serializing the topological structure of the three-dimensional compound molecule to obtain a topological sequence; obtaining a three-dimensional coordinate of each atom according to the sequence of the atoms in the topological structure to obtain a molecular conformation sequence; discretizing the size of the target nanoparticles to obtain a size sequence; integrating the topological sequence and the size sequence to obtain a first topological sequence, and integrating the molecular conformation sequence and the size sequence to obtain a first conformation sequence; and outputting the first topological sequence and the first conformation sequence to a neural network for feature extraction, and then performing prediction to obtain metal ions, organic compound additives and corresponding three-dimensional conformations of nanoparticles with specified sizes. The method has good application value.

The present invention provides: a method for preparing tissue-targeting nanoparticles, the method comprising a step for generating phospholipid nanoparticles, a step for generating mesenchymal stem cell-derived cell membrane nanoparticles, and a step for fusing the generated phospholipid nanoparticles and cell membrane nanoparticles to generate tissue-targeting nanoparticles; and tissue-targeting nanoparticles which are prepared by the method and comprise bisphosphonate-containing phospholipid nanoparticles and mesenchymal stem cell-derived cell membrane nanoparticles.

The invention belongs to the field of medicine, and particularly relates to a functional targeting nanoparticle as well as a preparation method and application thereof. The functionalized targeting nanoparticle is formed by self-assembly of a reduction response amphiphilic polymer, wherein the core of the functionalized targeting nanoparticle wraps a first chemotherapeutic drug and / or a macrophage regulator; the shell of the functionalized targeting nanoparticle adsorbs or chelates a second chemotherapeutic drug and / or a macrophage regulator; the surfaces of the functionalized targeting nanoparticles are modified with targeting molecules. The preparation method of the functionalized targeting nanoparticles is simple and efficient, and can effectively improve the accumulation of therapeutic drugs in tumor tissues. After treatment, the growth of tumors is significantly inhibited, and the infiltration abundance of anti-inflammatory tumor-related macrophages in tumor tissues is significantly reduced. Therefore, the functionalized targeting nanoparticles have a wide application prospect in the field of chemical immunotherapy of tumors.

This invention discloses a nanoparticle targeting bone tissue macrophages, its preparation method, and its applications, belonging to the field of biomedical technology. This invention employs a dual targeting mechanism, modifying the nanoparticle surface with bone-like peptide sequences and mannose-modified ligands, enabling the nanoparticles to specifically recognize macrophages in the bone tissue environment. The bone tissue macrophage-targeting nanoparticle system prepared by this invention can significantly improve targeting efficiency, anti-inflammatory effects, and tissue repair effects, while reducing systemic side effects, providing a new treatment approach for bone injury repair and showing promising clinical application prospects.

Provided herein are compositions, methods, and particles that include a peptide that targets placental chondroitin sulfate A (placental CSA), wherein the amino acid sequence of the polypeptide is as set forth in SEQ ID NO: 1 (EDVKDINFDTKEKFLAGLIVSFHEGKC).

The invention provides cardiac targeting nanoparticles as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The heart targeting nanoparticle provided by the invention contains the mesoporous silica nanoparticle, the myocardial targeting peptide and the SNX7 inhibitor, and can significantly improve the enrichment efficiency of a therapeutic drug at a heart part and reduce the exposure of the drug in non-target tissues, so that the heart function is effectively improved and the potential toxic and side effects are reduced; the nanoparticle system has excellent biocompatibility and stability, provides a new solution for precise treatment of heart failure, has wide clinical application prospects, and is expected to promote progress in the field of cardiovascular disease treatment.

The invention discloses a nanoparticle capturing method and related equipment, and the method comprises the following steps: uniformly covering a hydrogel solution on a sample liquid drop containing target nanoparticles on a glass slide to form a standard observation system; the hydrogel solution is temperature sensitive type hydrogel; determining a local area where the target nanoparticles are located in a standard observation system; starting a laser, controlling the laser to irradiate the local area, and regulating and controlling the laser power density of the laser, so that the temperature of the local area reaches and is maintained at the phase change temperature of the hydrogel solution; and adjusting laser parameters of the laser to generate stable hydrogel at the position of the target nanoparticle. According to the embodiment of the invention, the nano-particles can be captured by utilizing the characteristics of the temperature-sensitive hydrogel and combining a simple photo-thermal device, the influence caused by the limitation of capturing environment conditions or capturing sample properties is reduced, and the method can be widely applied to the technical field of nano-particle capturing.

This application discloses a tumor-targeting nanoparticle, its preparation method, and its application, belonging to the field of tumor drug technology. The tumor-targeting nanoparticle includes a drug and a delivery carrier loading the drug, wherein the drug is atorvaquinone. The delivery carrier is prepared by coupling an intermediate product with Sulfo-SMCC-BSA. The intermediate product is obtained by reducing a thiol-modified nucleic acid aptamer, wherein the nucleic acid aptamer is EGFR aptamer. This application utilizes a EGFR-targeting nucleic acid aptamer coupled with bovine serum albumin (BSA) to encapsulate atorvaquinone, successfully constructing a novel atorvaquinone nanomedicine. This improves the targeting ability of atorvaquinone and increases its water solubility, providing a new approach for the treatment of esophageal cancer with atorvaquinone nanomedicines.

The invention discloses preparation and application of macrophage targeting nanoparticles entrapped with a cell burial function accelerant. The medicine for enhancing the intercellular function of the macrophages is a liver X receptor stimulant, and the liver X receptor stimulant comprises at least one of T0901317, GW3965, Ouabagenin and LXR-623. The surface of the targeting nanoparticle is coated with a macrophage cell membrane, and meanwhile, a urokinase type plasminogen activator receptor (uPAR) targeting ligand is modified. The nanoparticle can be efficiently accumulated at an inflammatory focus part by utilizing a macrophage cell membrane, and the modified targeting ligand is used for targeting the macrophage with damaged cell burial function, so that the cell burial function of the macrophage is enhanced, the polarization of the macrophage to an anti-inflammatory phenotype is promoted, the release of anti-inflammatory and proinflammatory cytokines is regulated, and the anti-inflammatory effect of the nanoparticle is improved. And finally, the inflammatory microenvironment of a focus part is regulated and controlled, so that a good effect of treating autoimmune diseases is achieved.

The development of a new, targeted drug delivery paradigm coupled to improved PI3K inhibitors (e.g., PI3Kα inhibitors) represents a significant advance in cancer therapy. Provided herein are compounds, such as compounds of Formula (I) and (II), and pharmaceutically acceptable salts, hydrates, solvates, polymorphs, co-crystals, tautomers, stereoisomers, isotopically labeled derivatives, and prodrugs thereof. The compounds provided herein are PI3K (e.g., PI3Kα) inhibitors and are therefore useful for the treatment and / or prevention of various diseases (e.g., proliferative diseases such as cancer). Also provided herein are nanoparticles and nanogels (e.g., P-selectin targeting nanoparticles) comprising PI3K inhibitors, such a compound described herein. In certain embodiments, a nanoparticle or nanogel described herein encapsulates a compound described herein for targeting delivery to cancer cells or tumors.