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Photothermal conversion nanoparticles for targeted thermal elimination of Tregs (regulatory T cells), preparation and application

A nanoparticle and light-to-heat conversion technology, applied in the field of materials for tumor immune intervention, can solve problems such as high treatment costs, weak autoimmunity, and inability to effectively kill cancer cells

Inactive Publication Date: 2017-01-04
NANJING UNIVERSTIY SUZHOU HIGH TECH INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it must be recognized that due to the weak autoimmunity of tumor patients, even if they benefit from this type of therapy, their own anti-tumor immune response is still unable to effectively kill cancer cells
In order to achieve the desired therapeutic effect, drugs that block immunosuppression must be combined with stimulating anti-tumor immunotherapy, which increases the cost of treatment

Method used

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  • Photothermal conversion nanoparticles for targeted thermal elimination of Tregs (regulatory T cells), preparation and application
  • Photothermal conversion nanoparticles for targeted thermal elimination of Tregs (regulatory T cells), preparation and application
  • Photothermal conversion nanoparticles for targeted thermal elimination of Tregs (regulatory T cells), preparation and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1), weigh WCl 6 200 mg, dissolved in 50 mL of ethylene glycol under nitrogen protection, stirred and dissolved at room temperature to obtain a golden yellow solution, then added 300 mg of polyacrylic acid polymer with a molecular weight of 5KDa, stirred until completely dissolved, and then under nitrogen protection at 140°C Stir the reaction. After the system is heated, it is dark brown at first, then turns blue-green after 30 minutes, and finally turns dark blue after 1 hour, and remains unchanged. Continue heating for 1.5 hours, then cool down to 80 ° C, add 50 mL of deionized water, Continue to stir and react for 1 h, stop heating, centrifuge and purify the reacted solution, discard the supernatant, wash and disperse the product with deionized water, repeat this washing process three times, and finally obtain the purified product, which is dark blue W 18 o 49 Particle powder, TEM shows that its average particle size is 4.9nm.

[0030] (2) The prepared tungsten ox...

Embodiment 2

[0033] 1), weigh WCl 6 200 mg, dissolved in 50 mL of ethylene glycol under nitrogen protection, stirred and dissolved at room temperature to obtain a golden yellow solution, then added 300 mg of polyacrylic acid polymer with a molecular weight of 5KDa, stirred until completely dissolved, and then under nitrogen protection at 140°C Stir the reaction. After the system is heated, it is dark brown at first, then turns blue-green after 30 minutes, and finally turns dark blue after 1 hour, and remains unchanged. Continue heating for 1.5 hours, then cool down to 80 ° C, add 50 mL of deionized water, Continue to stir and react for 1 h, stop heating, centrifuge and purify the reacted solution, discard the supernatant, wash and disperse the product with deionized water, repeat this washing process three times, and finally obtain the purified product, which is dark blue W 18 o 49 Particle powder, TEM shows that its average particle size is 4.9nm.

[0034] (2) The prepared tungsten oxi...

Embodiment 3

[0037] 1), weigh WCl 6 200 mg, dissolved in 50 mL of ethylene glycol under nitrogen protection, stirred and dissolved at room temperature to obtain a golden yellow solution, then added 300 mg of polyacrylic acid polymer with a molecular weight of 5KDa, stirred until completely dissolved, and then under nitrogen protection at 140°C Stir the reaction. After the system is heated, it is dark brown at first, then turns blue-green after 30 minutes, and finally turns dark blue after 1 hour, and remains unchanged. Continue heating for 1.5 hours, then cool down to 80 ° C, add 50 mL of deionized water, Continue to stir and react for 1 h, stop heating, centrifuge and purify the reacted solution, discard the supernatant, wash and disperse the product with deionized water, repeat this washing process three times, and finally obtain the purified product, which is dark blue W 18 o 49 Particle powder, TEM shows that its average particle size is 4.9nm.

[0038] (2) The prepared tungsten oxi...

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Abstract

The invention discloses photothermal conversion nanoparticles for targeted thermal elimination of Tregs. Hydrophilic functional groups grafted with long cycles and functional groups of targeted Tregs are arranged on the surfaces of the nanoparticles, and the particle sizes of the nanoparticles are within 2 to 100 nanometers; the nanoparticles comprise tungsten oxide nanoparticles or gold nanoparticles in a non-stoichiometric ratio, wherein the molecular formulae of the tungsten oxide nanoparticles in the non-stoichiometric ratio are WOx; the particle sizes of the tungsten oxide nanoparticles are within 2 to 100 nanometers; when the targeted nanoparticles are the gold nanoparticles, the particle sizes of the targeted nanoparticles are within 1 to 200 nanometers, and the shapes of the targeted nanoparticles are one or more of spheres, triangles or rod-like structures; according to the invention, the Tregs are removed from tumor tissues, so that the inhibition of the Tregs for the activity of cytotoxic T cells can be blocked, furthermore, heat produced by a photothermal therapy stimulates the activation of the cytotoxic T cells in peripheral blood to weaken the immune tolerance of tumors and further stimulate the anti-tumor immune response, thereby achieving a specific killing effect on tumor cells.

Description

technical field [0001] The invention belongs to the technical field of materials for tumor immune intervention, and specifically relates to the application of regulatory T cell-targeted nanoparticles and its preparation. Background technique [0002] As a therapy with good body tolerance and high lesion selectivity, tumor immunotherapy has been regarded as the fourth pole besides the three traditional tumor therapies, and has become a model of personalized treatment. However, the remission rate and overall survival based on immunotherapy are unsatisfactory, which is in sharp contrast to a series of impressive results obtained by immunotherapy in vitro experiments. With the deepening understanding of the tumor microenvironment, cells with a phenotype of CD4+CD25+FoxP3+ called regulatory T cells (Regulatory T cells, Tregs) have attracted widespread attention. The function of Tregs in the body is mainly to inhibit the excessive activation of cell-killing T cells and the presen...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K39/44A61K47/32A61K47/48A61P35/00C01G41/02
CPCA61K41/0052A61K39/44A61K47/32C01G41/02C01P2004/04C01P2004/64A61K2300/00
Inventor 胡勇霍达陈娇
Owner NANJING UNIVERSTIY SUZHOU HIGH TECH INST
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