31 results about "DNA single strand" patented technology

The invention discloses an ultrasensitive immunodetection method based on adjacent nucleic acid amplification signal amplification in the technical field of medical detection. The ultrasensitive immunodetection method comprises the following steps: step 1, immune binding; step 2, enzyme digestion release: releasing a hairpin loop DNA single strand; step 3, complementation and enzyme ligation: adding T4 ligase to cyclize the circular DNA single chain; step 4, rolling circle amplification; and step 6, detecting, and quantifying and positioning by qPCR (quantitative polymerase chain reaction) and an in-situ oligonucleotide fluorescence labeling detection method. According to the present invention, the hairpin ring structure on the oligonucleotide DNA chain coupled to the antibody protects the complementary sequence so as to avoid the generation of the non-specific circular DNA single chain, and the rolling circle amplification technology is combined to amplify the detection signal, such that the detection background signal intensity is inhibited while the specificity of the detection signal is enhanced so as to further improve the detection sensitivity.

The invention provides an FSH33 peptide modified DNA tetrahedron nano delivery system as well as a preparation method and application thereof, and aims to provide a safe and efficient antioxidant nano drug and optimize the enrichment efficiency to promote ovarian aging and ovarian tissue damage repair. The system is a nano-scale nucleic acid compound composed of a tetrahedral DNA frame structure and an FSH targeting peptide. The tetrahedral DNA frame structure is a microscopic 3D stereoscopic tetrahedral structure formed by self-assembly of DNA single chains of a specific nucleic acid sequence, wherein each side extends out of a viscous end used for being connected with an FSH peptide. The research finds that the DNA tetrahedron can safely and effectively improve the ovarian endocrine function, ovarian reserve, fertility and the like of mice in a drug protection group, has no obvious toxic or side effect on all organs and tissues of the whole body, and can promote the repair of ovarian oxidative damage.

The application discloses a functional nucleic acid probe and a preparation method and application thereof. The preparation method comprises the following steps: forming a DNA sheet by self-assembly of a first DNA single strand and a second DNA single strand, and then combining the DNA sheet with a molecular beacon probe to prepare the functional nucleic acid probe; wherein the end of the first DNA single strand and the end of the second DNA single strand are complementary to each other; and the molecular beacon probe comprises an aptamer sequence of foodborne pathogenic bacteria. The fluorescence polarization method based on the functional nucleic acid probe provided by the application has the advantages of high sensitivity, strong specificity, short detection time and the like, and can be used for self-immediate monitoring of the content of foodborne pathogenic bacteria in drinking water or the content of foodborne pathogenic bacteria in environmental water, and has great application value and market value in family health and environmental protection and the like.

The application belongs to the technical field of biosensing and specifically relates to a biosensor for detecting active acetylcholinesterase and application thereof. The biosensor comprises nanotubes with nanometer apertures at tips for generating current blocking signals; and a ring bundle DNA tetrahedron carrier for loading a solution containing active acetylcholinesterase to be detected, wherein the ring bundle DNA tetrahedron carrier is TDN1 or TDN4, the sequences of the four DNA single strands in TDN1 are shown as SEQ ID NO. 1 to SEQ ID NO. 4, and the sequences of the four DNA single strands in TDN4 are shown as SEQ ID NO. 1, SEQ ID NO. 3, SEQ ID NO. 5 and SEQ ID NO. 6. The application utilizes a closed-loop DNA tetrahedron nanostructure anchored at two ends of an aptamer to generate a difference in current signals in a nanometer hole before and after the active AChE is combined, so that high-sensitivity and high-selectivity detection of the active AChE at a single-molecule level is achieved.

The invention relates to a polyarginine-DNA nanotube material as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. Aiming at the technical problems of unstable structure, low cell uptake rate and easy degradation by nuclease caused by dependence on magnesium ions in the existing DNA nano-material, the invention provides a nano-tube material formed by self-assembly of four DNA single chains (Y1-Y4) according to a molar ratio of 1: 3: 3: 3 induced by polyarginine. The material can load p65 siRNA to form a composite preparation which is used for preparing a medicine for inhibiting lung inflammation. According to the polyarginine-DNA nanotube material disclosed by the invention, the stability and the cell uptake efficiency of the material are remarkably improved; the higher the polymerization degree of arginine is, the stronger the anti-inflammatory activity is; the compound preparation synergistically inhibits the expression of a proinflammatory factor through a dual mechanism of blocking an inflammatory pathway by polyarginine and silencing a p65 signal by p65 siRNA, and provides an efficient delivery carrier for the treatment of acute lung injury.

This invention belongs to the field of biomedical technology, specifically relating to a DNA hydrogel with a kissing hairpin cross-linking structure, its preparation method, and its applications. The first end of the DNA single strand is connected to a first sticky end carrying a nucleic acid aptamer that specifically recognizes TNF-α, and the second end is connected to a second sticky end. The two hairpin structures form an integrated hairpin structure through complementary base pairing at their sticky ends. The loop regions of multiple integrated hairpin structures are cross-linked through complementary base pairing to form a DNA hydrogel with a three-dimensional network backbone. This application utilizes the self-assembly of anti-TNF-α nucleic acid aptamers to form a hydrogel via DNA kissing hairpin cross-linking, achieving controllable drug release. Compared to various TNF-α monoclonal antibodies and fusion protein biopharmaceuticals, anti-TNF-α nucleic acid aptamers offer the advantages of both good targeting and low cost; the self-assembly of the hydrogel via DNA kissing hairpin cross-linking allows for a wider range of adjustable release and higher safety.

The present disclosure provides a tetrahedral framework nucleic acid modified by aptamer and transforming growth factor β3 and a preparation method thereof, belonging to the field of biomedicine. The tetrahedral framework nucleic acid modified by aptamer and transforming growth factor β3 combines transforming growth factor β3 through disulfide bonds, and sequences of four DNA single strands of the tetrahedral framework nucleic acid are shown in SEQ ID NO: 1-4, one of the four DNA single strands of the tetrahedral framework nucleic acid also comprises an extended aptamer sequence, and the extended aptamer sequence is shown in SEQ ID NO: 5.

The invention discloses a DNA cube-sialidase conjugate as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The main body of the conjugate is a DNA cube, and is formed by DNA single chains of an AB chain, an AE chain, an AD chain, an AC chain, an a chain, a chain b and an x chain which are respectively shown as SEQ ID NO: 1-7 through base complementary pairing assembly; the 5'end of the chain a or the chain b is complementary with a DNA cube support chain to form double chains, the 3 'end is free in a single-chain form, so that four nonadjacent vertexes of the DNA cube respectively extend to form a single-chain DNA section, and the 3' end of each single-chain DNA section is connected with sialidase. Sialic acid on the cell surface is subjected to targeted degradation through the DNA cube-sialidase conjugate, and the DNA cube-sialidase conjugate can be used for targeted degradation of sugar immune checkpoints on the surface of tumor cells, has high targeting property and action efficiency, and is expected to be used for enhancing immunotherapy.

The invention discloses a preparation method and application of a fluorescence sensing array based on an MOF material. The preparation method comprises the following specific steps: step 1, preparing Zr-MOF; step 2, obtaining single-stranded DNA1, DNA2, DNA3 and DNA4, and marking carboxyl fluorescein at 5'ends of the single-stranded DNA1, DNA2, DNA3 and DNA4; carrying out centrifugal treatment on the labeled DNA single chain, and respectively adding a TBE buffer solution to prepare a single-chain DNA1 solution, a single-chain DNA2 solution, a DNA3 solution and a DNA4 solution for later use; and 3, respectively adding the Zr-MOF prepared in the step 1 into the single-stranded DNA1 solution, the single-stranded DNA2 solution, the DNA3 solution and the DNA4 solution prepared in the step 2, and incubating at room temperature to obtain the fluorescent sensing array.

The application belongs to the technical field of biological medicine. More specifically, it relates to a preparation and application of in-situ imaging DNA-PROTAC based on strand displacement activation. The novel DNA-PROTAC provided by the application is designed by three modules, which are respectively recruitment module A (E3 ligase ligand-linker-clicking group), locking module B (aptamer-shielded chain double-stranded complex) and activation module C (DNA single strand), three physically separated components, so that the degradation activity is completely silent in the delivery stage. Only after the drug reaches the target area, the in-situ assembly of recruitment module A and locking module B can be triggered by adding activation module C to trigger the strand displacement reaction, thereby effectively avoiding the non-specific activity of traditional degrading agents in the whole body circulation, and significantly reducing the off-target toxicity. In addition, the application converts the large conjugate into multiple independent components with medium and small molecular weights by physical splitting, overcoming the macromolecular delivery barrier.

The present invention relates to a method and kit for removing linker self-linking products during sequencing library construction and for constructing a sequencing library. In particular, provided is a method and kit for the selective cleavage of DNA double strands and DNA single strands, a method and kit for selective cleavage by using DNA-RNA-DNA:cDNA heterozygous chains and DNA:cDNA double strands, and a method and kit for DNA and RNA library co-construction.

The invention provides application of DNA tetrahedral framework nucleic acid in preparation of a medicine for preventing and / or treating fetal alcohol spectrum disorder, and belongs to the field of biological medicine. The DNA tetrahedral framework nucleic acid provided by the invention is formed by DNA single chains with sequences as shown in SEQ ID No.1-4 through base complementary pairing and self-assembly. The DNA tetrahedral framework nucleic acid can cross the placenta barrier, alleviate alcohol-induced neural crest cell apoptosis and oxidative stress injury, alleviate Et-OH-induced ROS level rise in vitro by regulating an Nrf2 / HO-1 pathway, and has a protection effect on fetal alcohol spectrum disorder craniofacial malformation and intrauterine growth retardation. The DNA tetrahedral framework nucleic acid provided by the invention has a good application prospect in preparation of drugs for preventing and / or treating fetal alcohol spectrum disorders.

The present invention discloses a molecular beacon-peptide probe and its application, the molecular beacon-peptide probe comprises a loop part complementary to the target miRNA, a stem part comprising a double-stranded section and a single-stranded section; the 3'-terminal A base of the double-stranded section is connected with biotin, and the T base complementary thereto is connected with a reporter polypeptide; the single-stranded section extends along a polynucleotide on one side of the double-stranded section connected to the reporter polypeptide, which contains a nickase recognition site and a base modified with a thiophosphate bond at the end. After the molecular beacon-peptide probe loop part binds to the target miRNA, a DNA-RNA hybrid double-strand is formed, and a new DNA is synthesized under the action of a DNA polymerase to replace the miRNA, forming a DNA-DNA hybrid ring, which is cut off with a base modified with a thiophosphate bond under the action of a nickase, exposing a nuclease recognition site, and the nuclease cuts one side of the single-stranded section into fragments and frees the DNA single-stranded section complementary to the loop part and the reporter polypeptide, and the DNA single-stranded section enters the next cycle, and after the cycle ends, the reporter polypeptide is quantified by mass spectrometry SRM.

The present invention relates to the technical field of biological detection, in particular to a DNA tetrahedral fluorescent probe based on multivalent spatial pattern recognition and the use thereof. The fluorescent probe is of a tetrahedral structure, and comprises five DNA single strands, three DNA long strands and three DNA short strands complementary to the DNA long strands, wherein the three DNA long strands comprise a CY5 dye modifying the 3' end, and the three DNA short strands comprise a BHQ2 dye modifying the 3' end. The DNA tetrahedral fluorescent probe of the present invention can achieve multivalent recognition of a circular RNA and structurally matches same, and thus has enhanced specificity and sensitivity of circular RNA detection and is suitable for imaging and detection of circular RNAs.

A SERS-electrochemical dual-mode sensor for miRNA detection, a preparation method thereof and an application thereof, wherein the SERS sensing mode is realized by extracting the intensity of the SERS characteristic peak of methylene blue MB, and the electrochemical sensing mode is realized by testing the MB peak current by square wave voltammetry; the sensing substrate is obtained by modifying a hairpin DNA single strand and an auxiliary strand on the surface of a silver nanorod array substrate, and when the target miR-106a is present, a stable LW / T / DNAzyme three-strand complex is formed on the substrate surface with the first reagent DNAzyme and the auxiliary single strand LW in the sensing substrate; then, under the activation of the second reagent, the first reagent is triggered to cut the hairpin DNA single strand H1 in the sensing substrate on the substrate surface, and the remaining H1 fragment after cutting further binds to a SERS / electrochemical dual-mode probe modified with a signal molecule methylene blue MB, thereby outputting SERS and electrochemical signals. The dual-mode sensor detects miR-106a in serum at attomole and femtomolar levels, respectively, meeting the requirements of high sensitivity and reliable detection.

The present invention discloses an ultrasensitive immunoassay method based on proximity nucleic acid amplification signal amplification in the field of medical detection technology, comprising the following steps: 1. immune binding; 2. enzymatic cleavage and release to release a hairpin DNA single strand; 3. complementary and enzymatic ligation to cyclize the circular DNA single strand by adding T4 ligase; 4. rolling circle amplification; and 6. detection, quantification and localization by qPCR and in situ oligonucleotide fluorescent labeling detection methods. The present invention uses the hairpin structure on the oligonucleotide DNA chain coupled to the antibody to protect the complementary sequence from generating nonspecific circular DNA single strands, and combines the rolling circle amplification technique to amplify the detection signal, thereby suppressing the intensity of the detection background signal while enhancing the specificity of the detection signal, further improving the sensitivity of the detection.

The present invention discloses a natural cyclic peptide RA-V & DNA tetrahedron immune adjuvant and its preparation method and application, which belong to the field of biomedicine. The immune adjuvant includes a Rubiaceae type cyclic peptide compound RA-V and a DNA tetrahedron, and the DNA tetrahedron is a DNA tetrahedron with an arm length of 15-30bp formed by a DNA single strand. The present invention constructs a nanostructure assembled from 4 DNA single strands and RA-V, which specifically activates the sGAS-STING pathway through the recognition of double-stranded DNA by cGAS protein, thereby enhancing the body's immune function and preventing diseases such as viral infections and tumors. At the same time, by utilizing the good biological properties of DNA nanostructures, RA-V & DNA tetrahedron can effectively target disease lesions and exert the immunotherapy effect of the disease, which has great application prospects.

The invention belongs to the technical field of biosensors, and particularly relates to a fluorescence-colorimetric dual-mode biosensor and a detection method and application of valvular endonuclease 1, in particular to a fluorescence-colorimetric dual-mode biosensor based on a cleavage-induced strand displacement reaction and an exponential rolling circle amplification reaction. Comprising a DNA branch structure of a 5'overhanging end, a hairpin substrate and a circular substrate, wherein the DNA branch structure of the 5'overhanging end is formed by hybridizing three DNA single chains D1, D2 and D3; the circular substrate is a circular substrate with three gap sites and a cytosine-rich sequence. The biosensor shows high sensitivity to FEN1, the fluorescence mode detection limit is 1.55 * 10 <-3 > UmL <-1 >, the colorimetric mode detection limit is 0.011 UmL <-1 >, the inhibition effect of an inhibitor can be effectively evaluated, the FEN1 activity in a cancer cell nuclear extract can be effectively detected, and the biosensor is a potential FEN1 activity analysis tool.

The invention relates to DNA-silk fibroin hybrid hydrogel as well as a preparation method and application thereof. The method comprises the following steps: mixing a Y-type DNA single strand and an L-type DNA single strand in a buffer solution to obtain a DNA solution, and forming a DNA supramolecular network by cross-linking cohesive ends of the Y-type DNA single strand and the L-type DNA single strand; the preparation method comprises the following steps: mixing methacrylated silk fibroin and a photoinitiator with a DNA solution to obtain a DNA-silk fibroin premixed solution; mnO2 nanoparticles and Melatonin are added into the DNA-silk fibroin premixed solution, and a hydrogel solution is obtained; and carrying out photo-crosslinking on the hydrogel solution under the irradiation of ultraviolet light, so as to obtain the DNA-silk fibroin hybrid hydrogel loaded with MnO2 nanoparticles and Melatonin. Compared with the prior art, the introduction of the silk fibroin network can provide mechanical properties for the DNA hydrogel network and reduce the cost; the MnO2 nanoparticles can be used for remarkably removing ROS in cells; the Melatonin can be used for remarkably promoting autophagy of damaged mitochondria in cells.

The invention relates to the technical field of biological detection, in particular to a single nucleotide mutation detection probe and method integrating a dynamic signal and an accumulated signal. The single nucleotide mutation detection probe comprises a DNA hairpin H1, an RNA base embedded hairpin Sub, a DNA single strand E1, a DNA single strand E2 and a DNA hairpin H2, wherein the DNA hairpin H1, the RNA base embedded hairpin Sub, the DNA single strand E1 and the DNA single strand E2 form a DNA enzyme structure; the 5'terminal of the DNA hairpin H2 is modified with carboxyl molecules, and the 3 'terminal of the DNA hairpin H2 is modified with ferrocene molecules. The hairpin probe recognition technology is combined with DNA enzyme, the purpose of cyclic detection is achieved through the thermodynamic chain competition effect, the sensitivity of single base mutation detection is improved through an electrogenerated chemiluminescence detection method for indirectly detecting a mononucleotide mutant by recognizing an intermediate product chain in a cyclic reaction, and the detection sensitivity of the mononucleotide mutant is improved. And the accuracy of single base mutation recognition is enhanced.

The invention discloses a space-limited double-foot DNA nano-motor with enhanced dynamics and a preparation method and application thereof. The preparation method comprises the following steps: annealing a specific DNA single chain to form a Cy5-substrate probe, a Cy3-substrate probe, a double-foot walker 1 and a double-foot walker 2; and respectively assembling the two substrate probes and the gold nanoparticles through a freezing method to obtain the DNA nano motor 1 and the DNA nano motor 2. The nano motor, a corresponding biped walker and a fuel chain form a detection system which is used for detecting messenger RNA (Ribonucleic Acid). The space confinement effect of the gold nanoparticles and the proximity effect of the biped walker are utilized, entropy-driven catalytic reaction kinetics is remarkably accelerated, ultra-high-sensitivity detection of survivin mRNA and TK1 mRNA is achieved, and the detection limits reach 501 aM and 314 aM respectively. And rapid and high-contrast in-situ imaging can be carried out on double mRNA in living cells.

The invention discloses a method for enhancing DNA specific binding based on acoustic driving, and relates to the field of microfluidics and biomedicine. The method comprises the following steps: preparing a micro-channel of which the side wall contains grooves which are arranged at equal intervals in a staggered manner, attaching the micro-channel to a glass slide, and pasting a piezoelectric ceramic piece on the glass slide; a PDDA solution is introduced into the microchannel, then a metal nanoparticle solution is introduced, deionized water is used for washing, metal nanoparticles distributed in a single layer in the microchannel are obtained, and then a DNA single strand is introduced into the microchannel; turning on a signal generator, connecting an electrode of the signal generator with an electrode of the piezoelectric ceramic piece, and introducing an SERS probe complementary with the DNA single strand. Bubbles are captured in the grooves in the side wall of the micro-channel, when acoustic driving is carried out, films with the captured bubbles start to oscillate, and bubble oscillation causes a micro-flow phenomenon, so that the collision frequency of two complementary DNA chains is increased, and the specific binding of DNA is enhanced.

The invention relates to the technical field of molecular biological experiments, in particular to a nucleic acid scavenger which comprises a solution A and a solution B. The solution A is an active oxygen source component in the nucleic acid scavenger, the main component of the solution A is urea peroxide (0.25%-3%), and the solution A can generate excessive oxygen free radicals and effectively destroy DNA single-chain and double-chain structures. The liquid B is a synergistic and auxiliary component in the nucleic acid scavenger, the main component is horse radish peroxidase (0.1%-1%), urea peroxide can be promoted to quickly release high-activity oxygen free radicals, and the purpose of scavenging nucleic acid molecules is achieved. According to the method, nucleic acid pollution including genome DNA and nucleic acid amplification product pollution in the laboratory can be effectively eliminated, chemical pollution to the environment cannot be caused, the method has the advantages of being non-toxic, harmless and easy and convenient to operate, and a safer and more effective anti-pollution scheme is provided for the molecular biology laboratory.

This invention discloses a molecular switch-type nucleic acid framework fluorescent probe and its application in the detection of ATP molecules, a biomarker for depression, belonging to the field of biodetection technology. The fluorescent probe of this invention has a tetrahedral structure, comprising three complementary DNA single strands and four additional DNA single strands forming the tetrahedral structure. The three complementary DNA single strands include a Cy3 dye modified with a central backbone, a phosphate group modified at the 5' end, and a Cy5 dye modified at the 3' end. The fluorescent probe of this invention is activated by ATP, rapidly responding to ATP to emit a fluorescent signal, and is deactivated by an endonuclease, automatically returning to its initial state. This probe enables dynamic cyclic detection of ATP molecules and can directly perform rapid, highly sensitive, and highly selective quantitative analysis of the purine neurotransmitter ATP in complex biological samples such as plasma and urine, providing an effective means for early screening of neurological diseases.

The invention discloses a preparation method of a DNA tetrahedron-small nucleic acid drug compound suitable for industrial production, which comprises the following steps: adding four DNA single strands into a TM buffer solution, maintaining the temperature at 70 DEG C for 10 minutes, cooling to 4-25 DEG C, adding an antisense strand of siRNA, and maintaining the temperature for more than 20 minutes to obtain the DNA tetrahedron-small nucleic acid drug compound. One of the four DNA single strands is connected with a positive-sense strand of siRNA (small interfering Ribonucleic Acid). According to the preparation process provided by the invention, the yield of the TDNs-small nucleic acid drug compound can exceed 50%, the purity can reach 80% or above, and under the condition of a conventional TDNs preparation process, the TDNs-small nucleic acid drug has an obvious degradation process, so that the yield and the purity of the product are greatly influenced, and the requirements of process production cannot be met.

The invention relates to the technical field of DNA calculation, and discloses a design method of a DNA half-subtracter and the DNA half-subtracter. The method comprises the following steps: acquiring a long DNA single strand and two short DNA single strands; the method comprises the following steps: simultaneously performing complementary hybridization on a long DNA single chain and two short DNA single chains on the basis of a DNA base complementary pairing principle to obtain a DNA three-chain composite structure with two toe domains, and taking the DNA three-chain composite structure as an AND gate logic block; the two AND gate logic blocks are connected in parallel to form an exclusive-OR logic circuit; an exclusive-OR logic circuit and an AND gate logic block are connected in parallel to form a DNA half-subtracter. The DNA half reducer is stable in structure, the design process does not need participation of biological enzyme, the working mechanism is simple, and the use cost is low.

The present application relates to a kind of polyarginine-DNA nanotube material and its preparation method and application, belong to the field of biological medicine.The existing DNA nanomaterial is dependent on magnesium ion, which leads to unstable structure, low cell uptake rate and easy degradation by nuclease.The present application is formed by four DNA single strands (Y1-Y4) self-assembly according to the molar ratio 1:3:3:3 nanotube material induced by polyarginine.The material can load p65 siRNA to form a complex preparation, which is used for preparing a drug for inhibiting lung inflammation.The polyarginine-DNA nanotube material of the present application significantly improves the stability and cell uptake efficiency of the material;The higher the degree of polymerization of arginine, the stronger the anti-inflammatory activity;The complex preparation inhibits the expression of pro-inflammatory factors through the double mechanism of blocking inflammatory pathways by polyarginine and silencing p65 signal by p65 siRNA, providing an efficient delivery vector for the treatment of acute lung injury.

A modifiable hydrogel material has main chain polymers which are modified with anchor modules in the form of predetermined functionalized single strands of DNA. The main chain polymers can be crosslinked with one another by intermolecular DNA double strand formation. A DNA sequence of the anchor modules has a predetermined number of specific sequence positions N with different base combinations and / or is blocked by a temperature-dependent DNA blocking strand. In a method for producing a hydrogel with the modifiable hydrogel material, DNA modules in the form of free DNA single strands or DNA modules in the form of predetermined DNA single strand pairs which bind to anchor modules in a complementary manner are employed for crosslinking the main chain polymers, which form an intermolecular DNA double strand at a common binding domain.

The invention relates to a DNA topological tetrahedral structure as well as a preparation method and application thereof. The DNA topological tetrahedral structure has the following characteristics: 1) four sulfo-modified thymine bases are extended at the 3'end of a DNA single strand corresponding to an expandable edge in the DNA topological tetrahedral structure, so that the DNA single strand can resist hydrolysis from 3'to 5 'of exonuclease III; and 2) modifying a phosphate group at the 5'end of a DNA single strand corresponding to a non-expanded side in the DNA topological tetrahedral structure, hybridizing the phosphate group with a complementary strand and connecting the phosphate group with the complementary strand end to end, so that the DNA single strand can be connected into a ring by T4DNA ligase. Compared with the prior art, the topological tetrahedron prepared by the method disclosed by the invention has more stable expandable performance, can be quickly and efficiently prepared, and has a good application prospect.

The application discloses an RNA response controlled-release DNA nanocarrier system, which comprises a DNA nanotube, a drug effective component and a nanodevice lock; the DNA nanotube comprises a tube wall and the nanodevice lock; the tube wall is unfolded in a rectangular shape; the nanodevice lock comprises a plurality of sealing chains, each of which is composed of two partially complementary DNA single strands, and the two DNA single strands are connected to two long sides of the tube wall; the sealing chains can undergo an isothermal chain displacement reaction with target RNA, open the nanodevice lock and unfold the tube wall; and the drug effective component is loaded in the inside of the DNA nanotube. The DNA nanotube is not easy to be enzymatically hydrolyzed, has high biocompatibility and is easy to be endocytosed by cells, and is used for wrapping the RNA drug with poor stability, so that the RNA drug is completely and sufficiently delivered to a target position; under the action of high expression of the RNA at the target position, the nanodevice lock on the DNA nanotube is rapidly opened, and the RNA drug is released.