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Hydrogel based on aptamer linking and preparation method and application thereof

A nucleic acid aptamer and hydrogel technology, which is applied in the direction of medical preparations, liquid delivery, and pharmaceutical formulations of non-active ingredients, can solve the problems of tissue toxicity, side effects, damage, and drug blocking, and achieve good biocompatibility sexual effect

Active Publication Date: 2014-08-13
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the clinical treatment of cancer is mainly through Western medicine surgery, radiotherapy and chemotherapy combined with traditional Chinese medicine. Tissues will also produce greater toxic side effects or damage; at the same time, tumor cells are prone to multidrug resistance to chemotherapy drugs, triggering autoimmune reactions to clear drugs or block their efficacy

Method used

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  • Hydrogel based on aptamer linking and preparation method and application thereof
  • Hydrogel based on aptamer linking and preparation method and application thereof
  • Hydrogel based on aptamer linking and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] A method for preparing a hydrogel based on nucleic acid aptamer links, such as figure 1 As shown, it is achieved through the following steps:

[0036] (1) Dissolve single-stranded DNA strand A S-A and strand B S-B with a specific base sequence in ultrapure water to obtain stock solutions of S-A and S-B, wherein the base sequence of S-A is 5'-acrydite-AAA ACA CCA CCG AGA T-3', as shown in SEQ ID NO.1, the base sequence of S-B is 5'-acrydite-AAA ATC ACA GAT GAG T-3', as shown in SEQ ID NO.2, the base sequence of S-A The stock solution of the stock solution and the stock solution of S-B were dissolved in 10mM buffer solution containing 4% acrylamide monomer containing 200mM NaCl, pH7.4 Tris hydrochloride buffer solution, pumped to vacuum, and the solution was stored in a dry In the desiccator, add 10% ammonium persulfate solution with a final volume of 1.4% and a 5% tetramethylethylenediamine solution with a final volume of 1.4%, and react in a desiccator for 15 minutes t...

Embodiment 2

[0043] A method for preparing a hydrogel based on nucleic acid aptamer links, such as figure 1 As shown, it is achieved through the following steps:

[0044](1) Dissolve single-stranded DNA strand A S-A and strand B S-B with a specific base sequence in ultrapure water to obtain stock solutions of S-A and S-B, wherein the base sequence of S-A is 5'-acrydite-AAA ACA CCA CCG AGA T-3', as shown in SEQ ID NO.1, the base sequence of S-B is 5'-acrydite-AAA ATC ACA GAT GAG T-3', as shown in SEQ ID NO.2, the base sequence of S-A The stock solution of the stock solution and the stock solution of S-B were dissolved in 10mM buffer solution containing 4% acrylamide monomer containing 200mM NaCl, pH7.4 Tris hydrochloride buffer solution, pumped to vacuum, and the solution was stored in a dry In the desiccator, add 10% ammonium persulfate solution with a final volume of 1.4% and 5% tetramethylethylenediamine solution with a final volume of 1.4%, and react in a desiccator for 20 minutes to o...

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Abstract

The invention discloses a hydrogel based on aptamer linking and a preparation method and application thereof. The hydrogel can connect different aptamers to realize the active targeting drug release to different cancer cells. The preparation method comprises the following steps: (1) connecting DNA single strand and acrylamide to obtain polymer chains, PS-A and PS-B; and (2) mixing the obtained PS-A, PS-B, an aptamer chain and an anticancer drug to obtain a drug loaded hydrogel. The hydrogel during preparation is directly mixed anticancer drugs, realizes drug entrapment during a self-assembly process. The hydrogel completes drug entrapment in the self-assembly process, is capable of specific recognition of cancer cells, achieves the targeting release of anticancer drug, and is suitable for targeted cancer therapy.

Description

technical field [0001] The invention relates to the preparation and application of a hydrogel, in particular to a hydrogel based on a nucleic acid aptamer link and a preparation method and application thereof. Background technique [0002] At present, the clinical treatment of cancer is mainly through Western medicine surgery, radiotherapy and chemotherapy combined with traditional Chinese medicine. Tissues will also produce greater toxic side effects or damage; at the same time, tumor cells are prone to multidrug resistance to chemotherapy drugs, triggering autoimmune reactions to clear drugs or block their efficacy. [0003] With the increasing improvement of drug delivery systems, the design and synthesis of targeted drug carriers play a vital role in medicine and healthcare. At the same time, innovations in targeted drug carrier materials and nanotechnology have jointly promoted the advancement of drug delivery systems. Therefore, it is of great significance to constru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K47/26A61P35/00
Inventor 夏建飞王宗花蔡峰张菲菲毕赛夏霖夏临华李延辉夏延致
Owner QINGDAO UNIV
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