Hydrogel based on aptamer linking and preparation method and application thereof
A nucleic acid aptamer and hydrogel technology, which is applied in the direction of medical preparations, liquid delivery, and pharmaceutical formulations of non-active ingredients, can solve the problems of tissue toxicity, side effects, damage, and drug blocking, and achieve good biocompatibility sexual effect
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Embodiment 1
[0035] A method for preparing a hydrogel based on nucleic acid aptamer links, such as figure 1 As shown, it is achieved through the following steps:
[0036] (1) Dissolve single-stranded DNA strand A S-A and strand B S-B with a specific base sequence in ultrapure water to obtain stock solutions of S-A and S-B, wherein the base sequence of S-A is 5'-acrydite-AAA ACA CCA CCG AGA T-3', as shown in SEQ ID NO.1, the base sequence of S-B is 5'-acrydite-AAA ATC ACA GAT GAG T-3', as shown in SEQ ID NO.2, the base sequence of S-A The stock solution of the stock solution and the stock solution of S-B were dissolved in 10mM buffer solution containing 4% acrylamide monomer containing 200mM NaCl, pH7.4 Tris hydrochloride buffer solution, pumped to vacuum, and the solution was stored in a dry In the desiccator, add 10% ammonium persulfate solution with a final volume of 1.4% and a 5% tetramethylethylenediamine solution with a final volume of 1.4%, and react in a desiccator for 15 minutes t...
Embodiment 2
[0043] A method for preparing a hydrogel based on nucleic acid aptamer links, such as figure 1 As shown, it is achieved through the following steps:
[0044](1) Dissolve single-stranded DNA strand A S-A and strand B S-B with a specific base sequence in ultrapure water to obtain stock solutions of S-A and S-B, wherein the base sequence of S-A is 5'-acrydite-AAA ACA CCA CCG AGA T-3', as shown in SEQ ID NO.1, the base sequence of S-B is 5'-acrydite-AAA ATC ACA GAT GAG T-3', as shown in SEQ ID NO.2, the base sequence of S-A The stock solution of the stock solution and the stock solution of S-B were dissolved in 10mM buffer solution containing 4% acrylamide monomer containing 200mM NaCl, pH7.4 Tris hydrochloride buffer solution, pumped to vacuum, and the solution was stored in a dry In the desiccator, add 10% ammonium persulfate solution with a final volume of 1.4% and 5% tetramethylethylenediamine solution with a final volume of 1.4%, and react in a desiccator for 20 minutes to o...
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Description
Claims
Application Information
- IPC
- A61K9/06; A61K47/26; A61P35/00
- Inventors
- 夏建飞; 王宗花