Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Electrochemical detection method for microRNAs based on rolling circle amplification mediated palladium nano-particles

A technology of palladium nanoparticles and rolling circle amplification, applied in the field of electrochemical detection of microRNA by palladium nanoparticles, can solve the problems of process redundancy, time-consuming, inconvenient operation, etc., achieve good selectivity, high sensitivity, improve transmission The effect of sensibility

Active Publication Date: 2019-05-03
SHANDONG UNIV
View PDF1 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as far as the inventors of the present disclosure are aware, this process of labeling signal indicators is redundant, time-consuming, and inconvenient to operate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Electrochemical detection method for microRNAs based on rolling circle amplification mediated palladium nano-particles
  • Electrochemical detection method for microRNAs based on rolling circle amplification mediated palladium nano-particles
  • Electrochemical detection method for microRNAs based on rolling circle amplification mediated palladium nano-particles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0064] Reagents and materials

[0065] HPLC-purified DNA and miRNA (shown in Table 1) were purchased from Shanghai Sangon Biotechnology Co., Ltd., China. Phi29 DNA polymerase, T4 DNA ligase, diethyl pyrocarbonate (DEPC), tris-hydrochloric acid (Tris-HCl), ethylenediaminetetraacetic acid (EDTA), 4-(2-hydroxyethyl)piperene A solution of sodium oxazine-1-ethanesulfonate (HEPES) and a mixture of deoxyribonucleotide triphosphates (dNTPs) was purchased from Shanghai Sangon Biotechnology Co., Ltd., China. Sodium borohydride (NaBH 4 ), potassium ferricyanide (K 3 Fe(CN) 6 ) and potassium ferrocyanide (K 4 Fe(CN) 6 ) was purchased from Sinopharm Holding Chemical Reagent Co., Ltd. Sodium hypochloropalladate (Na 2 PdCl 4 ), 6-mercapto-1-hexanol (MCH) and tris(2-carboxyethyl) liquid phosphine hydrochloride (TCEP) were purchased from Aladdin, Shanghai, China. TE buffer (10 mM Tris-HCl, 100 mM NaCl, 1.0 mM EDTA, pH 8.0) was used to dilute the DNA. Tris buffer (40mM Tris-HCl, 10mM ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure provides an electrochemical detection method for microRNAs based on rolling circle amplification mediated palladium nano-particles. MiRNAs to be tested are specifically bound toa DNA probe, and the exposed primer sequence A2 of the DNA probe and circular template DNA are subjected to rolling circle amplification so as to obtain a guanine-rich DNA single strand. The guanine-rich DNA single strand is used as a template, palladium salt and a reducing agent are added for reduction reaction, and then electrochemical detection is performed. The method of the present disclosure is useful for detecting miRNA-21, has a linear range of 50 aM to 100 fM, has an ultra-low detection limit of 8.6 aM, and has excellent selectivity at the same time.

Description

technical field [0001] The disclosure belongs to the technical field of electrochemical detection, and relates to an electrochemical detection method for microRNA based on palladium nanoparticles mediated by rolling circle amplification. Background technique [0002] The statements herein merely provide background information related to the present disclosure and may not necessarily constitute prior art. [0003] MicroRNAs (miRNAs), as a promising biomarker for early diagnosis and prognosis of non-invasive diseases (especially cancer), provide important information for understanding cancer initiation, metastasis and further biomedical applications. The analysis of miRNA is of great significance. However, to the best of the knowledge of the inventors of the present disclosure, accurate detection of miRNAs is still challenging due to their intrinsic properties such as small size, low expression level in biological samples, and similar sequence of family members. In order to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6844C12N15/11G01N27/26
Inventor 朱烨张翠玲温凯
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com