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Golden nanoparticle controllably assembled with DNA dendrites and preparation method and application thereof

A gold nanoparticle and dendrimer technology, applied in the field of DNA dendrimer and its preparation, can solve problems such as the introduction of complex branched chains, and achieve the effects of simple and stable structure, increased space interval, good innovation and application potential

Pending Publication Date: 2017-02-22
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acquisition of most dendrimers requires a multi-step synthesis and purification process, as well as complex branch introduction steps

Method used

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  • Golden nanoparticle controllably assembled with DNA dendrites and preparation method and application thereof
  • Golden nanoparticle controllably assembled with DNA dendrites and preparation method and application thereof
  • Golden nanoparticle controllably assembled with DNA dendrites and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Construction of DNA sequences used by DNA dendrimers

[0047] The DNA sequences used to design and construct DNA dendrimers (see Table 1) were purchased through Invitrogen Biotechnology.

[0048] Table 1 DNA sequences used to construct DNA dendrimers

[0049]

Embodiment 2

[0050] Example 2 DNA sequence used to prepare DNA monofunctionalized gold nanoparticles

[0051] The DNA sequences (see Table 2) used to design and prepare DNA monofunctionalized gold nanoparticles were purchased through Invitrogen Biotechnology.

[0052] Table 2 DNA sequences used to prepare DNA monofunctionalized gold nanoparticles

[0053]

Embodiment 3

[0054] The preparation method of embodiment 3 Y-shaped DNA (Y-DNA)

[0055] Equal amounts of three DNA sequences (Y na , Y nb and Y nc ) were mixed together in phosphate buffer solution (100 mM NaCl, 50 mM sodium phosphate, pH 8.0) so that the final concentration of each DNA strand was 20 mM. Then the mixture was heated to 95°C for 5 minutes, and then cooled to 4°C at a rate of 1°C per minute to finally obtain the Y-DNA assembly structure.

[0056] The thermal stability of Y-DNA was determined by ultraviolet spectroscopy (such as image 3 shown). The experimental results showed that all the melting points of Y-DNA (T m ) are higher than 60 °C, which also means that the monomers of these DNA dendrimers are stable at room temperature.

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Abstract

The invention provides a controllable assembling method of golden nanoparticle, particularly discloses a synthesis strategy based on divergence method of the dendrites. Through the gradual incorporation of 'three arms' structure of Y shaped DNA monomer, the DNA dendrites with discrete DNA 'sticky end' and different algebra is formed by itself. At the same time the applicant of the golden nanoparticle hybridizes golden nanoparticles loaded with a line of complementary DNA single strand with the dendrites of different algebras to form an aggregation structure of discrete golden nanoparticle with controllable quantity. The technical scheme of the method bears huge potential value in the applications of the spatial positioning of funciontalized nanoparticle and precise establishment of multivalent nanomaterial.

Description

technical field [0001] The invention relates to a DNA dendritic molecule and a preparation method thereof, and a method for controlling the self-assembly of nano gold particles by using the DNA dendritic molecular structure. The invention belongs to the field of nano-biological materials and has potential applications in drug carriers, biological detection and the like. Background technique [0002] With the development of nanotechnology, the self-assembly of nanoparticles into complex nanostructures has attracted extensive research interest. In the past ten years, gold nanoparticles have been widely used in nano-optoelectronic materials, and many of their properties, such as surface plasmon resonance, largely depend on the interaction between adjacent particles. The "bottom-up" self-assembly strategy provides a very suitable method for the precise arrangement and regulation of the distance and geometry between nanoparticles. [0003] Understanding nanoparticle assembly be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10A61K47/26A61K47/02C12Q1/68
CPCA61K47/02A61K47/26C12N15/10C12N2310/52C12Q1/6876
Inventor 陈平
Owner HUAIYIN TEACHERS COLLEGE
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