Drug for preventing and treating myocardial ischemia reperfusion injury or treating cardiac ischemic diseases
A technology for ischemic disease and reperfusion injury, applied in cardiovascular system diseases, gene therapy, drug combination, etc., can solve the problems of tFNAs research reports that have not been seen, and achieve the effect of reducing oxidative damage, increasing proliferation, and reducing release
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Embodiment 1
[0034] Synthesis and identification of embodiment 1 tFNA
[0035] 1. Synthesis method
[0036] Dissolve the four DNA single strands (S1, S2, S3, S4) in TM Buffer (10mM Tris-HCl, 50mM MgCl2, pH=8.0), the final concentration of the four DNA single strands is 1000nM, mix well, heat rapidly to 95°C and keep After 10 minutes, the temperature was quickly lowered to 4°C and maintained for more than 20 minutes to obtain tetrahedral skeleton nucleic acid.
[0037] The sequences of the four single strands (5'→3') are as follows:
[0038] S1: ATTTATCACCCGCCATAGTAGACGTATCACCAGGCAGTTGA
[0039] GACGAACATTCCTAAGTCTGAA (SEQ ID NO. 1)
[0040] S2: ACATGCGAGGGTCCAATACCGACGATTACAGCTGCTACAC
[0041] GATTCAGACTTAGGAATGTTCG (SEQ ID NO. 2)
[0042] S3: ACTACTATGGCGGGTGATAAAACGTGTAGCAAGCTGTAATC
[0043] GACGGGAAGAGCATGCCCATCC (SEQ ID NO. 3)
[0044] S4: ACGGTATTGGACCCTCGCATGACTCAACTGCCTGGTGATAC
[0045] GAGGATGGGCATGCTCTTCCCG (SEQ ID NO. 4)
[0046] 2. Identification
[0047] After the syn...
experiment example 1
[0049] Experimental example 1 In vitro model experiment of cardiomyocyte ischemia-reperfusion
[0050] 1. Method
[0051] 1.1 Ischemia-reperfusion treatment (SIR)
[0052] H9C2 cells in the logarithmic growth phase were collected and divided into control group, SIR group, and SIR+tFNAs group. After 24 hours of adherent growth, the three groups discarded the original culture medium (high glucose DMEM), washed with PBS for 3 times, and the control group was treated with The DMEM medium containing 1% FBS continued to be cultured in a normoxic incubator.
[0053] SIR, SIR+tFNAs groups were added artificial hypoxia solution (10mM deoxyglucose, 4mM 4-hydroxyethylpiperazineethanesulfonic acid, 137mM NaCl, 0.49mM MgCl2, 12mM KCl, 20mM lactate, 0.9mM CaCl 2 2H2 O, pH 6.5), placed in an incubator containing 94% nitrogen, 1% oxygen, and 5% carbon dioxide and cultured for 3 hours; the anoxic solution was sucked off, and the SIR group was added with DMEM medium containing 1% FBS, SIR+tFN...
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