Fluorescent probe for detecting cell hydroxyl radical, and synthesis method and use

Abstract

The invention provides a fluorescence probe, the synthetic method and the use which is to test the cell hydroxyl free radical. The process is: a. heat and chloroauric acid of 150.0 weight concentration and back flow, with mixing, the sodium citrate of 5.0-7.0 is added into the solution to back flow for 10-20min, then to cool in room temperature to get the golden nanometer particle solution; b. mix the particle with the DNA single chain with 5'end modified fluorescein and 3'end modified sulfydryl according to 1:200-500, then to set in room temperature for 1224h, last to adjust the pH by the phosphoric buffer; next to mix the mixture with the 0.5-5mol / L NaCl according to 1:0.1-0.3(V / V) which is set in room temperature for 35-45h; last to centrifugate to remove the upper solution and the deposition is solved in the 0.1-0.3mol / L phosphoric buffer which is the fluorescence probe solution.

Description

Technical field:

[0001] The invention belongs to the fields of biological detection technology and clinical medical detection, and relates to a fluorescent probe for detecting cell hydroxyl free radicals; at the same time, it relates to a synthesis method of the fluorescent probe; in addition, it also relates to applications of the fluorescent probe. Background technique:

[0002] Various free radicals are produced in the metabolic process of life activities, and the aging of organisms and the occurrence of many diseases are closely related to free radicals. Free radicals can mediate many important reaction processes of life activities, participate in cellular immune function, destructively modify the chemical structure of tissue cells, and damage the morphology and membrane function of normal tissue cells (Witz G., Proc.Soc.Exp.Biol Med. 1991, 198:675; Feig D.I., Reid T.M., Leob L.A., Cancer Res. 1994, 54:1890s; Finkl T., Holbrook N.J., Nature 2000, 408:239). Hydroxyl free...

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