Preparation method of ratio electrochemical miR3123 aptamer sensor based on MOF (metal organic framework) compound

A metal-organic framework and aptamer sensor technology, applied in biochemical equipment and methods, material electrochemical variables, scientific instruments, etc., to achieve strong anti-interference ability, good selectivity, and high detection sensitivity

Active Publication Date: 2019-11-15
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Up to now, there have been no domestic and foreign literatures and patent reports on the use of black phosphorus nanosheets and thionine co...

Method used

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  • Preparation method of ratio electrochemical miR3123 aptamer sensor based on MOF (metal organic framework) compound
  • Preparation method of ratio electrochemical miR3123 aptamer sensor based on MOF (metal organic framework) compound

Examples

Experimental program
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Embodiment 1

[0018] The preparation method of the ratiometric electrochemical miR3123 aptasensor based on black phosphorus nanosheets and thionine co-doped copper-based metal-organic framework nanocomposites involved in this example, the schematic diagram of the preparation process and miR3123 detection principle is as followsfigure 1 As shown, the specific process steps are as follows:

[0019] Preparation of TH / Cu-MOF composite: Weigh 4 mg of copper nitrate trihydrate, 10 μL of trifluoroacetic acid, and 10 mg of polyvinylpyrrolidone into 12 mL of a mixed solvent containing 9 mL of N,N-dimethylformamide and 3 mL of ethanol, and stir Mixture A was formed evenly. Weigh 4mg of 4,4',4",4"'-(porphin-5,10,15,20-tetraalkyl)tetrabenzoic acid and 4mg of thionine into 3mL N,N-dimethyl In a mixed solvent of formamide and 1 mL of ethanol, stir evenly to form a mixed solution B. Add the mixed solution A to the mixed solution B dropwise, stir and sonicate for 10 minutes, then heat the A-B mixed soluti...

Embodiment 2

[0023] Example 2: The ratiometric electrochemical aptasensor based on black phosphorus nanosheets and thionine co-doped copper-based metal-organic framework nanocomposites involved in this example, its preparation process and schematic diagram of miR3123 detection principle, TH / Cu - The process steps for the preparation of MOF and BPNSs / TH / Cu-MOF composite are the same as in Example 1.

[0024] Other specific process steps are as follows:

[0025] The cross-linking agent Nafion was added dropwise on the surface of the polished and polished bare glassy carbon electrode, and then the BPNSs / TH / Cu-MOF composite dispersion was drop-coated to form the composite-modified glassy carbon electrode. Submerge the modified electrode in phosphate saline buffer containing 5 μM miR3123-corresponding DNA single-stranded aptamer Fc-DNA, incubate at 37°C for 2 h, then take out the electrode, and prepare aptamer-BPNSs on the surface of the electrode after natural drying / TH / Cu-MOF nanocomposites...

Embodiment 3

[0027] Example 3: The ratiometric electrochemical aptasensor based on black phosphorus nanosheets and thionine co-doped copper-based metal-organic framework nanocomposites involved in this example, the schematic diagram of its preparation process and the principle of miR3123 detection, TH / Cu - The process steps for the preparation of MOF and BPNSs / TH / Cu-MOF composite are the same as in Example 1.

[0028] Other specific process steps are as follows:

[0029] The cross-linking agent Nafion was added dropwise on the surface of the polished and polished bare glassy carbon electrode, and then the BPNSs / TH / Cu-MOF composite dispersion was drop-coated to form the composite-modified glassy carbon electrode. The modified electrode was submerged in phosphate saline buffer containing 8 μM miR3123-corresponding DNA single-stranded aptamer Fc-DNA, incubated at 37°C for 2 h, then the electrode was taken out, and aptamer-BPNSs were prepared on the surface of the electrode after natural dryin...

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Abstract

The invention discloses a preparation method of a ratio electrochemical miR3123 aptamer sensor based on a Cu-MOF (copper-based metal organic framework) compound doped with BPNSs (black phosphorusnanosheets) and TH (thionine). The method comprises steps as follows: TH and a Cu-MOF precursor are subjected to a reaction for preparation of TH/Cu-MOF, and BPNSs/TH/Cu-MOF is prepared by drip coating ofthe BPNSs and applied to an electrode by drip coating; DNA single-stranded aptamers marked with Fc (ferrocene) are adsorbed on the BPNSs, and aptamer-BPNSs/TH/Cu-MOF is prepared; a target moleculemiR3123is bonded with the DNA single-stranded aptamers Fc-DNA, Fc-DNA is enabled to be separated from the BPNSs, and therefore, Fc electrochemical signals are weakened while TH signals are slightly affected; the ratio electrochemical miR3123aptamer sensor is constructed by fitting linear relation of current peak intensity ratio IFc/ITH and miR3123 concentration.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of metal-organic framework composite materials and ratiometric electrochemical aptasensors, in particular to a ratiometric electrochemical miR3123 aptasensor based on a copper-based metal-organic framework nanocomposite co-doped with black phosphorus nanosheets and thionine. A method for preparing a biosensor, the prepared sensor can be used for highly sensitive and highly selective quantitative detection of miR3123 in biomedical samples. Background technique: [0002] MicroRNAs, abbreviated as miRNAs, are a class of non-coding single-stranded RNA molecules encoded by endogenous genes with a length of about 22 nucleotides. In the process of cell differentiation and biological development, it is involved in the regulation of gene expression after transcription in animals and plants. Because of their remarkable functions, miRNAs have aroused extensive research interests of scientific and techn...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
CPCG01N27/3275G01N27/3277G01N27/48B05D1/30C12Q1/6886C12Q2600/178G01N27/3276G01N27/3278B05D1/00C12Q1/6825G01N27/308C12Q2563/113C12Q2537/165
Inventor 桂日军孙玉娇金辉姜晓文
Owner QINGDAO UNIV
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