Preparation method of ratio electrochemical miR3123 aptamer sensor based on MOF (metal organic framework) compound
A metal-organic framework and aptamer sensor technology, applied in biochemical equipment and methods, material electrochemical variables, scientific instruments, etc., to achieve strong anti-interference ability, good selectivity, and high detection sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0018] The preparation method of the ratiometric electrochemical miR3123 aptasensor based on black phosphorus nanosheets and thionine co-doped copper-based metal-organic framework nanocomposites involved in this example, the schematic diagram of the preparation process and miR3123 detection principle is as followsfigure 1 As shown, the specific process steps are as follows:
[0019] Preparation of TH / Cu-MOF composite: Weigh 4 mg of copper nitrate trihydrate, 10 μL of trifluoroacetic acid, and 10 mg of polyvinylpyrrolidone into 12 mL of a mixed solvent containing 9 mL of N,N-dimethylformamide and 3 mL of ethanol, and stir Mixture A was formed evenly. Weigh 4mg of 4,4',4",4"'-(porphin-5,10,15,20-tetraalkyl)tetrabenzoic acid and 4mg of thionine into 3mL N,N-dimethyl In a mixed solvent of formamide and 1 mL of ethanol, stir evenly to form a mixed solution B. Add the mixed solution A to the mixed solution B dropwise, stir and sonicate for 10 minutes, then heat the A-B mixed soluti...
Embodiment 2
[0023] Example 2: The ratiometric electrochemical aptasensor based on black phosphorus nanosheets and thionine co-doped copper-based metal-organic framework nanocomposites involved in this example, its preparation process and schematic diagram of miR3123 detection principle, TH / Cu - The process steps for the preparation of MOF and BPNSs / TH / Cu-MOF composite are the same as in Example 1.
[0024] Other specific process steps are as follows:
[0025] The cross-linking agent Nafion was added dropwise on the surface of the polished and polished bare glassy carbon electrode, and then the BPNSs / TH / Cu-MOF composite dispersion was drop-coated to form the composite-modified glassy carbon electrode. Submerge the modified electrode in phosphate saline buffer containing 5 μM miR3123-corresponding DNA single-stranded aptamer Fc-DNA, incubate at 37°C for 2 h, then take out the electrode, and prepare aptamer-BPNSs on the surface of the electrode after natural drying / TH / Cu-MOF nanocomposites...
Embodiment 3
[0027] Example 3: The ratiometric electrochemical aptasensor based on black phosphorus nanosheets and thionine co-doped copper-based metal-organic framework nanocomposites involved in this example, the schematic diagram of its preparation process and the principle of miR3123 detection, TH / Cu - The process steps for the preparation of MOF and BPNSs / TH / Cu-MOF composite are the same as in Example 1.
[0028] Other specific process steps are as follows:
[0029] The cross-linking agent Nafion was added dropwise on the surface of the polished and polished bare glassy carbon electrode, and then the BPNSs / TH / Cu-MOF composite dispersion was drop-coated to form the composite-modified glassy carbon electrode. The modified electrode was submerged in phosphate saline buffer containing 8 μM miR3123-corresponding DNA single-stranded aptamer Fc-DNA, incubated at 37°C for 2 h, then the electrode was taken out, and aptamer-BPNSs were prepared on the surface of the electrode after natural dryin...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com