8 results about "Tetrazolium salt" patented technology

The invention discloses a reagent for detecting the activity of isocitrate dehydrogenase. The reagent consists of a first reagent and a second reagent, the first reagent comprises an electron carrier, isocitric acid or isocitrate, divalent metal ions, a first reagent buffer solution, a first reagent additive, a first reagent preservative and a first reagent surfactant; the divalent metal ions comprise metal ions; the second reagent comprises a dye which comprises tetrazolium salt and has an oxidation state and a reduction state, a cofactor, a second reagent buffer solution, a second reagent additive, a coenzyme stabilizer, a second reagent surfactant and a second reagent preservative; the pH is buffered to 7-9.5 and 5-9 by the first buffer solution and the second buffer solution respectively; the first reagent additive and the second reagent additive comprise divalent metal salt, sugar, sugar alcohol, protein, chelating agent and basic amino acid; the first and second reagent surfactants comprise nonionic and anionic surfactants; the first reagent preservative and the second reagent preservative comprise sodium azide or isothiazolinones. The invention further discloses a corresponding detection method.

The invention relates to the technical field of energetic material synthesis, and particularly provides 5, 5 '-azotetrazole salt and a preparation method thereof.The preparation method comprises the following steps that 1, pretreated carbon cloth is immersed in a mixed solution formed by dissolving cobalt nitrate hexahydrate, ammonium fluoride and urea in deionized water, after a hydrothermal reaction, the carbon cloth is taken out and calcined in the air atmosphere, and a precursor is obtained; and finally, the cobaltosic oxide electrode material loaded on the carbon cloth is prepared. 2, dissolving 5-aminotetrazole in an alkaline solution to prepare an electrolyte, forming a three-electrode system by taking the electrolyte and cobaltosic oxide loaded on carbon cloth as a working electrode, a platinum sheet as a counter electrode and a mercury / mercuric oxide electrode as a reference electrode, and electrifying to carry out electrochemical reaction; 3, bromine water is dropwise added into the three-electrode system in the electrifying electrolysis process, hydrochloric acid is dropwise added to adjust the pH value after the reaction is finished, extraction and rotary evaporation are conducted, and the final product 5, 5 '-azotetrazole salt is obtained.

The present invention relates to a method for reducing WST-8 to formazan based on NAD(P)H for determining the susceptibility of certain bacterial isolates to infection by certain bacteriophages, thereby allowing direct observation of the results measured as cell death of the target bacteria. The reduction of tetrazolium salts to formazan by NAD(P)H is used to determine the metabolic activity of cells and as an indicator of cell viability; whereas WST-8 is used to qualitatively measure the bacteriophage killing activity of the target bacteria. The present invention thus belongs to the field of medical biology, in particular to in vitro laboratory test methods.

The invention discloses an oil spill degradation intermediate product in-situ detection kit and a use method thereof, and belongs to the field of environmental monitoring, the kit comprises an alkane degradation indication module, an aromatic hydrocarbon degradation indication module and a total petroleum hydrocarbon residue detection module, the alkane degradation indication module utilizes fatty alcohol dehydrogenase to catalyze a reaction between fatty alcohol and NAD, and the total petroleum hydrocarbon residue detection module is used for detecting total petroleum hydrocarbon residues. NADH reduces tetrazole salt to generate a purple formazan compound for colorimetric detection, and the aromatic hydrocarbon degradation indication module utilizes catechol dioxygenase to catalyze ring opening of catechol to generate a yellow semialdehyde compound for colorimetric detection. According to the total petroleum hydrocarbon residue detection module, fluorescence quenching is caused by an inclusion compound formed by fluorescein labeled cyclodextrin and petroleum hydrocarbon, the kit is matched with a portable photometer and data processing software, detection can be completed within 15 minutes on site, an evaluation index of the oil spill degradation efficiency can be calculated, the detection sensitivity reaches 5 milligrams per liter, and the detection time is short. And a rapid screening tool is provided for oil spill bioremediation engineering.

The present invention relates to a method based on the WST-8 reduction to formazan by NAD(P)H for determining the susceptibility of relevant bacteria isolates to certain bacteriophage infection allowing direct visualization of the results measured by the cell death of the target bacteria. The reduction of tetrazolium salts by NAD(P)H to formazan is used to determine the metabolic activity of cells, and as an indicator of cell viability, whilst WST-8 is used for the qualitative measurement of the phage killing activity of the targeted bacteria. Thus, the present invention falls within the field of medical biology, particularly in vitro methods of laboratory tests.

The present invention relates to a method of preparing an incubation medium for histochemical analysis of diamine oxidase in a native tissue sample. In a first step, phenazine methosulfate, preferably 0.5 to 1.0 mg phenazine methosulfate; an aqueous tetrazolium salt solution at a concentration of 0.01 to 0.2 wt. % and in a molar ratio of tetrazolium salt to phenazine methosulfate of 2:1 to 1 :2, preferably 1.5 to 2.5 mL 0.1 wt. % nitro blue tetrazolium chloride solution; an aqueous substrate solution comprising at least one D-amino acid at a concentration of 0.4 to 1.2 M and in a molar ratio of D-amino acid to phenazine methosulfate of 10:1 to 230:1, preferably 0.1 to 0.3 mL substrate solution comprising 1 M D-alanine and 0.5 M D-phenylalanine; and optionally, an aqueous cyanide salt solution at a concentration of 0.01 to 0.2 M and in a molar ratio of cyanide salt to phenazine methosulfate of 40:1 to 1 :4, preferably 0.1 to 0.3 mL 0.1 M potassium cyanide solution are mixed. In a second step, the incubation medium is illuminated for 1 to 10 min. to form an incubation medium for histochemical analysis of diamine oxidase in the native tissue sample.

This test piece is used for detecting an analyte in a sample by immunochromatography, the test piece comprising a matrix, and parts disposed on the matrix, which are: a label body-containing part that holds a label body that contains an enzyme and a first probe molecule capable of binding to an analyte, such that the label body can be eluted, or a label body adding part that adds the label body; a substrate-containing part that holds a color-developing substrate of the enzyme such that the color-developing substrate can be eluted; and an enhancer-containing part that holds a color-developing enhancer of the color-developing substrate such that the color-developing enhancer can be eluted. The enzyme is alkaline phosphatase, the color-developing substrate is 5-bromo-4-chloro-3-indolylphosphoric acid (BCIP), the color-developing enhancer is a tetrazolium salt, and the enhancer-containing part is disposed downstream of the substrate-containing part.

The present invention relates to a method of synthesizing copper (I) 5-nitrotetrazolate. The method involves a series of interconnected steps. Initially, a stock solution of sodium 5-nitrotetrazolate is prepared in water from 5-aminotetrazole nitrate, which is then preserved for later use in producing copper (I) 5-nitrotetrazolate thereby eliminating the need for immediate utilization. A nascent Copper (I) chloride is prepared separately. In a single step, the stock solution of sodium 5-nitrotetrazolate is coupled with the nascent copper (I) chloride, resulting in the formation of copper (I) 5-nitrotetrazolate.