49 results about "Tetrazolium salt" patented technology

The invention discloses a pressure steam sterilization chemical indicator and a preparation method thereof, belonging to the field of moist heat sterilization indication. The chemical indicator mainly includes two parts: chemical indicator ink and substrate. The weight composition of the active ingredients of the pressure steam sterilization chemical indicator is: 1-10 parts of tetrazolium salt, 5-20 parts of system stabilizer, 50-70 parts of solvent, 10-20 parts of binder, and 5 parts of alkaline auxiliary. ‑20 servings, and 1‑10 servings of antioxidants. The above active ingredients are mixed and stirred to obtain a uniform and viscous composition, which is then sent to a grinder for grinding to form a uniformly dispersed indicator ink. The indicator ink is printed on the substrate by flexographic printing, and after drying, the chemical indicator is obtained by pressure steam sterilization. The pressure steam sterilization chemical indicator provided by the present invention is exposed to the saturated pressure steam conditions of 121 ° C, 132 ° C and 134 ° C, and the color of the indicating ink changes from colorless to dark, which can be determined by the color depth of color development. Determine whether the sterilization is qualified.

Thiazolyl tetrazolium salts with increased solubility in aqueous solutions have alkylammonium alkoxy substituents, preferably trimethyl ammonium propoxy groups substituents, on phenyl rings attached to the tetrazolium ring. Thiazolyl tetrazolium salts with increased solubility in aqueous solutions have alkyl ammonium alkoxy substituents, preferably trimethyl ammonium propoxy groups substituents, on phenyl rings attached to the tetrazolium ring, e.g. the compounds of the general formula shown below. Formula (A-)a.

The invention discloses a class of anti-tumor compounds containing a triazole heterocyclic structure and an application thereof, belonging to the technical field of medicinal chemistry. The compounds containing the triazole heterocyclic structure introduce quintuple 1,2,3-triazole ring in the form of side chains through the amino condensation, bromination, nitrine substitution, link reaction and other reactions on the basis of naphthalimide parent structure, select tetrazolium salt reduction to test 7721 hepatocellular in the experiment of inhibiting proliferation of tumor cells, and also select sulfonyl rhodamine B protein staining method to test MCF-7 breast cancer cells. The experiment results show that the anti-tumor compounds have wide anti-tumor activity and good inhibition effect on the normal growth of tumor cells derived from different tissues of liver cancer, breast cancer and the like.

The invention belongs to the technical field of medical biological detection, and in particular relates to the use and method of using monosulfonic acid tetrazolium salt for microbial detection; the mixture reagent of novel monosulfonic acid tetrazolium salt and PMS derivatives is non-toxic and can be combined with microorganisms. The characteristics of co-incubation and color change are used to monitor bacteria in real time, providing a rapid detection method for bacterial properties; the detection reagent uses EZMTT monosulfonic acid tetrazolium salt detection system, which shows a good linear dose relationship in the number of viable bacteria; The reagent passes the absorbance value of the monosulfonic acid tetrazolium salt reactant at 450nm to obtain the EC 50 The curve was used to evaluate the bacterial viability.

The present invention relates to a method of visualizing biomolecules, having the steps of: a) providing a sample of immobilized biomolecules in a matrix and carry on the electrophoresis process; b) incubating the matrix of step a) in a solution containing a cyanine-derived molecule, for a time of 5 to 60 minutes, at room temperature, in a container preventing exposure to light, shaking the container at less of 75 rpm; c) transferring the matrix from step b) to a container with a solution having: at least one tetrazolium salt and incubating for a time of 15 to 120 minutes at room temperature under light exposure; d) removing the matrix with immobilized biomolecules from the previous step and washing with distilled water; and e) visualizing directly by the naked eye the biomolecules immobilized in the matrix.

The invention discloses a low-temperature fast preparation method of a perovskite luminous material of formamidine lead bromide nanometer crystals. According to the method, firstly, the raw materials required for preparation are poured into a flask, an ultrasonic cell disruptor is used for stirring the solution for making the materials into a nanometer crystal raw solution; then, acetonitrile and toluene are added; centrifugation is performed; supernatant fluid is removed; precipitates obtained after the centrifugation treatment are taken; then, the toluene is added into the precipitates so that the precipitates are dissolved; then, centrifugation is performed; supernatant fluid is removed; the precipitates obtained after the centrifugation treatment are taken; the FAPbX3 green light nanometer crystals are finally obtained. The FAPbX3 nanometer crystals prepared by using the process provided by the invention can realize stable photoluminescence being 530 to 535 nm; no inert gas and no high-temperature reaction condition are needed in the preparation process; the simple, efficient, low-temperature and great-batch production of the perovskite luminous material can be realized; the quantum yield reaches 80 percent or higher.