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Frame nucleic acid nano luminous body with customizable shape and preparation method and application thereof

A nucleic acid nano and luminescent technology, which is applied in the direction of nanotechnology, nanotechnology, nanotechnology, etc. for materials and surface science, can solve the problems of lack of size and shape of new nano-fluorescent luminophores in the field of fluorescence imaging, and achieve rapid synthesis , the effect of simple operation

Active Publication Date: 2019-09-20
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

[0004] The purpose of the present invention is to provide a framework nucleic acid nano-luminescent body with a customizable shape and its preparation method and application, so as to solve the lack of new nano-fluorescence in the field of fluorescence imaging in the prior art, with precise and controllable size and shape, and precisely quantified fluorescence intensity. Luminous Problems

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  • Frame nucleic acid nano luminous body with customizable shape and preparation method and application thereof
  • Frame nucleic acid nano luminous body with customizable shape and preparation method and application thereof
  • Frame nucleic acid nano luminous body with customizable shape and preparation method and application thereof

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Embodiment 1

[0032] According to a preparation method for constructing a framework nucleic acid nanoluminescent body with a customizable shape based on DNA nanotechnology provided by the present invention, four kinds of framework nucleic acid nano-hydrophobic cavities V1, V2, V3, and V4 with a customizable shape were synthesized in this example. Include the following steps:

[0033] Hydrophobic group 1 is modified on the 5' end of DNA single strand A' and DNA single strand B' respectively, wherein hydrophobic group 1 is -{(CH2)12}3-. All DNA was dissolved in Q water and quantified by UV spectrophotometer.

[0034] Polyacrylamide gel electrophoresis ( figure 2 ) The results showed that the migration rate of DNA single strands modified by hydrophobic groups was slower than that of unmodified DNA single strands, which was due to the modification of hydrophobic groups and the molecular weight of DNA single strands became larger.

[0035] Then, take clip1, clip2, clip3, clip4 and the DNA sin...

Embodiment 2

[0045] According to the preparation method for constructing nucleic acid nano-hydrophobic cavities with customizable framework shapes and nanoluminescent bodies based on DNA nanotechnology provided by the present invention, in this embodiment, synthesizing nano-hydrophobic cavities with customizable framework nucleic acids of different shapes includes the following steps:

[0046] All DNA single strands were dissolved in deionized water and quantified using a UV spectrophotometer.

[0047] The S1, S2, S3, and S4 strands were dissolved in 100 μL of TM buffer solution, the DNA single strand molar ratio was 1:1:1:1, and the TM buffer solution was Tris buffered saline solution with a magnesium ion concentration of 5 mM. After mixing evenly, the reaction system is placed in a PCR instrument for reaction, and the tetrahedral framework nucleic acid nano-hydrophobic cavity can be obtained. Wherein, the annealing procedure is 15 minutes at 95 degrees Celsius, and 15 minutes at 4 degree...

Embodiment 3

[0057] Hydrophobic group 2 modified shape customizable framework nucleic acid nano-hydrophobic cavity assembly aggregation-induced luminescent AIE-like molecules to form a shape customizable framework nucleic acid nano-luminescent body, including the following steps:

[0058] Aggregation-induced luminescence-like AIE molecules are added to the shape-customizable framework nucleic acid nano-hydrophobic cavity system modified by the hydrophobic group 2, wherein the aggregation-induced luminescence-like AIE molecules are in large excess. Mix the AIE molecule with the shape-customizable framework nucleic acid nano-hydrophobic cavity modified by the hydrophobic group 2 by vibration or ultrasound, so that the AIE molecule enters the shape-customizable framework nucleic acid nano-hydrophobic cavity modified by the hydrophobic group 2 through the hydrophobic force The shape of the cavity, which eventually forms a customizable framework for nucleic acid nanoluminescents, is modified by ...

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Abstract

The invention provides a frame nucleic acid nano luminous body with a customizable shape and a preparation method and application thereof. The preparation method comprises the following steps: S1, modifying hydrophobic groups on DNA single chains, and self-assembling the DNA single chains modified by the hydrophobic groups through DNA nano structure design to obtain a frame nucleic acid nano hydrophobic cavity with a customizable shape; and S2, mixing the frame nucleic acid nano hydrophobic cavity with hydrophobic fluorescent molecules or hydrophobic drug molecules dissolved in a specific solvent according to a certain mode to allow the hydrophobic fluorescent molecules or hydrophobic drug molecules to enter the frame nucleic acid nano hydrophobic cavity to prepare the frame nucleic acid nano luminous body with the customizable shape. The frame nucleic acid nano luminous body with the customizable shape not only solves the problem of lack of a novel nano fluorescent luminous body with precise and controllable size and appearance and precise quantifiable fluorescence intensity in the fluorescent imaging field, but also provides an excellent carrier for hydrophobic drug molecule delivery and a new research idea for the development of fluorescent imaging technology.

Description

technical field [0001] The invention belongs to the field of biological nanomaterials, and in particular relates to a framework nucleic acid nanoluminescent body with a customizable shape, a preparation method and application thereof. Background technique [0002] Bio-optical imaging provides a large amount of biological information for biological and medical research, such as the characteristics and morphology of specific cell regions, and the expression and distribution of specific biomolecules. important value. Among them, fluorescence imaging technology can realize in situ and real-time monitoring of target molecules. Compared with other imaging methods, it has the advantages of no damage, high specificity and high sensitivity. Therefore, fluorescence imaging technology has important applications in the fields of disease diagnosis, drug metabolism tracking, and revealing the microscopic effects of biomolecules. At present, fluorescence imaging has been widely used in t...

Claims

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Application Information

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IPC IPC(8): C09K11/06C09K11/02A61K49/00A61K47/26B82Y5/00B82Y30/00B82Y40/00C12N15/10
CPCA61K47/26A61K49/0054A61K49/0093B82Y5/00B82Y30/00B82Y40/00C09K11/02C09K11/06C12N15/10
Inventor 樊春海冯灵玉沈建磊王丽华
Owner SHANGHAI JIAO TONG UNIV
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