118results about How to "Realize in situ detection" patented technology

The invention discloses a transparent flexible surface enhanced Raman active substrate and a preparation method thereof. The preparation method of the substrate comprises the following steps: taking a transparent flexible material such as polybutylene terephthalate (PET) plastics as a substrate, performing O2 plasma etching to form a nano wire array, loading gold, silver and other precious metal nanoparticles on the nano wire surface by virtue of a coating technology, and forming a three-dimensional nanometer structure array. The transparent flexible surface enhanced Raman active substrate provided by the invention is uniform in morphology and controllable in structure and has high-density electromagnetic hot spots in three-dimensional distribution. Therefore, the substrate has high sensitivity and reproducibility. The PET plastic film is high in flexibility and high in transparency, and incident laser and Raman scattered light easily penetrate through the substrate at low loss, so that the substrate can be used for in-situ detection of to-be-detected objects. The preparation method provided by the invention is simple in process, large-area preparation is easily realized, even roll-to-roll production process is permitted, and the cost is low.

The invention relates to a detection device and especially relates to an in-situ detection device for occurrence of hydrates in deposits. The CT in-situ detection device for microcosmic occurrence of hydrates in deposits comprises a pressure control system, a semiconductor temperature control system, an autoclave and an X-CT scanning system. The pressure control system is communicated with the autoclave. The semiconductor temperature control system is arranged at the bottom of the autoclave. The X-CT scanning system is arranged at two sides of the autoclave. The device achieves real-time detection of distribution of deposit particles, water, hydrates and free gas during hydrate formation/decomposition process.

A copper ion fluorescent probe and a preparation method and the use thereof relate to a fluorescence molecule probe, in particular to a copper ion fluorescence molecule probe and the preparation method and the application of the probe in the identification and detection of heavy metal. A copper ion fluorescence molecule probe with better effect which can test the heavy metallic copper ions in aqueous solution and the preparation method and the application are provided. Polyethyleneimine is allocated to be polyethyleneimine solution; the polyethyleneimine solution is taken out, filtered and poured into the lining of polytetrafluoroethylene which is sealed by a stainless steel cover; a solvent is distilled after reactions and the yellow viscous liquid-like copper ion fluorescent probe is gained. The copper ion fluorescent probe can be applied to the detection of the heavy metallic copper ions, in particular to the detection of the copper ions in biological tissues and environmental wastewater.

The invention discloses a fluorescence molecule probe which belongs to the technical field of chemical analysis and detection, and particularly relates to a zinc and copper molecule probe, a method for preparing the probe and the application of the probe in heavy metal ion identification and detection. As a 1-(7-acetamide-1, 8-naphthyridine)-2-(6-acetamide-pyridine)-vinyl compound, the zinc and copper ion fluorescence probe can be applied in ion detection and heavy metal ion detection for biological tissues, and has the advantages of rapidity, accuracy, convenience, good specificity and better prospect of application.

The invention belongs to the technical field of fault diagnosis of electrical equipment and relates to an in-situ detection method of fault characteristic gas dissolved in transformer oil. According to the method, single frequency single-mode-based transverse mode (TEM00) laser having wavelength of 532 nm irradiates a standard oil sample, the laser passes through a first high-pass filter and a space filter unit and then passes through a second high-pass filter and a lens, a Raman spectrum acquisition device acquires a standard oil sample Raman spectrogram, and after modeling, ethylene, acetylene and carbon dioxide concentrations in the oil sample to be detected are determined without oil and gas separation. The method has high accuracy and simple processes, can directly detect concentration of multiple fault characteristic gas dissolved in transformer oil and improves the accuracy of fault prediction.

The invention discloses a method for detecting mycobacterium tuberculosis by in-situ fluorescent loop-mediated isothermal nucleic acid amplification technology and a kit. Four specific primers are designed in a mycobacterium tuberculosis gyrB gene conservation area; and the mycobacterium tuberculosis can be specifically detected by using the four primers and combining the in-situ fluorescent loop-mediated isothermal nucleic acid amplification technology at the same time. The method realizes in-situ detection of a sample, does not need to increase bacteria, has high specificity and can finish amplification in less than 2 hours; the lowest detection limit reaches 10CFU/ml; and the detection rate of the sample reaches 99 percent. The method and the kit for identifying the mycobacterium tuberculosis are simple and convenient.

The invention relates to an in-situ detection device for the chlorophyll concentration of water and a detection method of the in-situ detection device. The in-situ detection device and the detection method aim at solving the problems that in an existing device, the excitation light path can be not adjusted, the fluorescence intensity is low, and the fluorescence detection accuracy and sensitivity are low due to scattering generated when water contains lots of impurities. A water sample chamber water inlet device is arranged at the bottom of a quartz water sample chamber, a stainless steel shell is arranged outside the quartz water sample chamber, a detector, a light filter and a focusing lens are arranged over the quartz water sample chamber, a light source, a light source transverse adjustment support, a light source transverse adjustment mechanism and a light source direction adjustment mechanism are arranged on the side face of the quartz water sample chamber, and the detector, the light source, the light source transverse adjustment mechanism and a light source incidence angle adjustment mechanism are connected with a host through wires. The method includes the steps that water background fluorescence signals measured under the off-state of the excitation light source are collected, then water fluorescence signals under the on-state of the excitation light source are collected, and background fluorescence interference is automatically subtracted through operation.

The invention discloses a precipitated alkaline phosphatase fluorescence probe, and a synthetic method and applications thereof. The synthetic method of the fluorescence probe comprises the following steps: 1, synthesizing a novel solid fluorescence dye (HTPQ); and 2, designing the dye to form the alkaline phosphatase probe (HTPQA). The applications comprise the response performance of the fluorescence probe to alkaline phosphatase in a buffer solution and the ability of fluorescence probe in in-situ detection of cell endogenous alkaline phosphatase and fluorescence signal stabilization indiffusion. The precipitated solid fluorescence dye is synthesized for the first time, so problems of no in-situ detection and easy signal diffusion of traditional fluorescence probes are solved; the fluorescence probe has solid fluorescence properties, can effectively reduce background fluorescence signals, and has improved sensitivity; and the fluorescence probe has the advantages of very good living cell dyeing effect, short dyeing time, high dyeing efficiency, and realization of in-situ detection of the cell endogenous alkaline phosphatase.

The invention discloses an atom magnetometer and an in-situ detection method for alkali metal atom air chamber pressure intensity thereof. The atom magnetometer comprises a light source, a polarizer,a quarter-wave plate, the alkali metal atom air chamber, a non-magnetic electric heating film, an optical micro-cavity, a micro-nano optical fiber, a beam splitter prism, a spectrograph and a signal processing circuit. And the optical micro-cavity is in contact with the micro-nano optical fiber and is integrated in the alkali metal atom air chamber. And the pressure in the alkali metal atom air chamber is calculated by measuring the resonant wavelength and the wavelength variation of the optical micro-cavity. According to the method, the signal of the atom magnetometer and the pressure signalof the alkali metal atom air chamber can be simultaneously extracted from the output signal of the alkali metal atom air chamber. The pressure intensity of the alkali metal atom air chamber can be detected while the atom magnetometer works normally, and the measurement precision is high. And meanwhile, no magnetic field noise is introduced into the optical micro-cavity. The method can also be usedin other quantum precision measurement systems using alkali metal atom gas chambers.

The invention belongs to the technical field of optical precision test and precision machining, and relates to a machining and detecting device and method for a large-aperture aspheric surface, which can be used for detecting and machining the surface shape of a large-sized high-precision aspheric element in a precise optical system. According to the invention, based on technologies such as a straight-line/rotating benchmark technology, the high-precision machining and detection of a large-sized aspheric element, which are based on a precise air-floatation rotation center and integrate machining and detecting functions, are realized by combining the functions of machining equipment and detecting equipment. According to the invention, a workpiece is polished repeatedly by a polishing joint arm according to set technological machining parameters, then contour parameters of a polished working surface are directly detected by a sensing measurement system on a measuring arm, and a computer measurement control system determines the machining path and polishing quantity of the polishing joint arm according to detected contour data, so that the machining efficiency and precision of the large-aperture aspheric element are improved.

The invention discloses a technique for directly reducing pellet ore by means of methanol. The technique for directly reducing the pellet ore by means of the methanol comprises the steps that the impedance change in the reduction process is tested by means of an in-situ impedance detecting system, and heating evaporation is conducted on methanol liquid; then methanol gas is mixed with hydrogen and nitrogen, and the gas mixture is introduced into an atmosphere reaction furnace at the temperature of 750-900 DEG C; the methanol is decomposed into reductive active gas at the high temperature, and the reduction reaction of the pellet ore is conducted through the reductive active gas, the hydrogen and the nitrogen jointly; and then wires connected to the two ends of a pellet are connected with an electrochemical detection device, and the impedance change of the pellet is monitored in real time after the gas mixture is introduced. According to the technique, the in-situ impedance detecting system is utilized and can be well coupled with an electrochemical testing system, and thus safety and reliability are achieved; the hydrogen produced by a hydrogen generator is used as a hydrogen source, so that environmental friendliness is achieved, and efficient utilization of energy resources can be achieved. In addition, the methanol is used for replacing part of the hydrogen, so that the cost is reduced, operation is easy, the technique is simple, the gas utilization rate is high, and the practical value in industrial production is quite high.

An original-position detecting device used for obtaining physical property parameter of deposit on surface of sea-bottom is prepared as fixing a water content probe on metal cylinder vertically or axially and connecting said probe to data collection and treatment unit by armoured cable as well as making probe head be extended out from said cylinder, considering to measure natural density of surface soil body simultaneously than also fixing a density probe being connected to data collection and treatment unit through armoured cable on said metal cylinder.

The invention relates to a method of ultrafast detection of antibiotic substances by combined voltage driven solid phase microextraction-raman spectroscopy. The method comprises the following steps: taking a nano golden particle wrapped porous silver wire as a working electrode, a saturated calomel electrode as a reference electrode and a platinum electrode as a counter electrode to form an electrode system, putting the electrode system in a water sample to be detected, applying -0.05 to -0.2V voltage to the electrodes, quickly enriching the antibiotic substances in the water sample onto the working electrode due to voltage driving to achieve in-situ solid phase microextraction, and performing exciting irradiation on the surface of the working electrode through a raman spectrometer for raman spectroscopy. The method increases a diffusion rate of the substances to be detected in the water sample; the nano golden particle wrapped porous silver wire is specific to the antibiotic substances, so that the antibiotic substances are enriched onto the working electrode directionally selectively; the enrichment detection speed is greatly increased; and actual applications in quick field detection and water sample pollutant surveillance are achieved.

Provided in the invention is an on-line monitoring device for fault gas in transformer oil based on fiber enhancement. The device comprises a laser, a laser beam expander, a dichroic mirror, a spatialfilter, a coupler, a nanometer-hole-modified kagome bandgap photonic crystal fiber, and a spectrometer. Laser is emitted by the laser; the laser is expanded by the laser beam expander and the expanded laser passes through the dichroic mirror; the coupler couples the laser to the nanometer-hole-modified kagome bandgap photonic crystal fiber that is arranged in transformer oil; Raman scattered light and a laser signal that are transmitted by the kagome bandgap photonic crystal fiber are returned to the dichroic mirror; the returned light and signal pass through the spatial filter; and then theprocessed light and signal are collected by a CCD of the spectrometer. According to the on-line monitoring device, no artificial oil and gas separation is needed; the accuracy is high; and the operation is simple. The fault gas in the transformer oil can be detected directly at the original position.

A visualized miRNA detecting method by utilizing an exonuclease reaction to generate primers and dendritic rolling circle amplification is disclosed. The method includes three steps. The first step is a step of primer generation, wherein after a hairpin-structured probe I is opened by a target miRNA, a single-chain part is degraded by an exonuclease I, and then RNA in a DNA:RNA hybrid chain is degraded by ribonuclease H to leave a short-chain DNA segment. The second step is a step of dendritic rolling circle amplification, wherein the single-chain DNA generated in the former step is connected to a long single-chain DNA probe to form a ring adopted as a template, first-stage rolling circle amplification is performed by adopting the single-chain DNA as a primer, and a hairpin-structured stem-loop structure II introduced into a rolling circle amplification system is opened by a product of the first-stage rolling circle amplification, thus performing multi-stage dendritic rolling circle amplification. The third step is a step of performing detection based on a G-quadruplex in a product of the rolling circle amplification. The method is a sensitive, economical, convenient and in-situ visualized miRNA detecting method.

The invention provides a preparation method of a high-power ultraviolet intensity detection material, relates to a preparation method of an ultraviolet intensity detection material, and is used for solving the problems that equipment adopted in a conventional ultraviolet intensity detection method is complicated, and the conventional detection method is complicated and high in cost. The preparation method provided by the invention comprises the following steps: dissolving a reactant containing a silver element in methyl alcohol, then adding an organic ligand, and stirring for 20-50 minutes to obtain a mixture A; then adding polyoxometallate containing rare earth elements and deionized water to the mixture A, and stirring for 30-45 minutes to obtain a mixture B; transferring the mixture B to a hydrothermal kettle, reacting for 72 hours at the temperature of 120 DEG C, cooling to room temperature, filtering, and putting obtained filtrate at the room temperature for 20 days to 25 days to obtain the ultraviolet intensity detection material, namely completing the preparation of the high-power ultraviolet intensity detection material. The high-power ultraviolet intensity detection material is applied to the field of ultraviolet intensity detection.

The invention provides an integrated and portable instrument for rapidly analyzing heavy metals as well as an analysis and detection method thereof. The instrument comprises the following components:a liquid sample introduction device (1), a pneumatic micro atomization system (2, 3, 4, 5, 6, 8, 9, 10), a corona discharge system (7, 11, 12, 13) and a spectrum system (14, 15, 16), wherein the pneumatic micro atomization system comprises a sheath gas carrying bottle (2), a secondary gas carrying bottle (3), a tee a (4), a tee b (5), a tee c (6), a fluid channel (8), a sheath gas channel (9), anda secondary gas channel (10); liquid to be measured in the liquid sample introduction device (1) enters the fluid channel (8) through a middle port of the tee a (4); the sheath gas carrying bottle (2) introduces the carrying gas into the sheath gas channel (9) through a middle port of the tee b (5); the secondary gas carrying bottle (3) introduces the secondary gas to the secondary gas channel (10) through a middle port of the tee c (6); the fluid channel (8), the sheath gas channel (9) and the secondary gas channel (10) are arrangement in a coaxial arrangement. One carrying gas channel is added to the analyzer, so that rapid and effective quantitative analysis of heavy metal samples can be guaranteed, and at the same time loss of sample ion beam is reduced, and accuracy and precision ofapparatus detection are improved.

The invention discloses a device and a method for detecting the disc repairing period of a polishing die. The device comprises an optical detecting component, a processor and a levelness measuring component. The optical detecting component uses structured light to detect the surface topography of the polishing die; and the processor calculates and processes to obtain the depth and width information of a groove in the surface of the polishing die through the received probe light comprising the surface topography information of the polishing die, so that the repairing period of the polishing dieis determined, and the maintaining time node of the polishing die is monitored. The device is simple in structure, precise in measurement and rapid in response, realizes in-situ detection of the discrepairing period of the polishing die, ensures the grinding activeness of the surface of the polishing die and improves the processing efficiency and the processing quality.

The invention provides and in-situ detection system for residual stress, and a detection method thereof. The residual detection system for the residual stress comprises an X-ray residual stress analyzer (1) and a workpiece force-applying test stand (2). The X-ray residual stress analyzer (1) is provided with an X-ray emission gun (11); and the X-ray residual stress analyzer (1) carries out the detection for the residual stress at a detection point (P) on a to-be-detected workpiece (10) through the X-ray emission gun (11). The workpiece force-applying test stand (2) comprises a stand frame (21), a workpiece holder (22), a force-applying pressure head (23) and a drive mechanism not shown in the figure, wherein the workpiece holder (22) is fixed on the stand frame (21); the workpiece holder (22) supports the to-be-detected workpiece (10); the drive mechanism is connected with the force-applying pressure head (23) and provides power needed by the force-applying pressure head (23); and the force-applying pressure head (23) applies a force to the to-be-detected workpiece (10). The magnitude of the force applied to the to-be-detected workpiece by the force-applying pressure head (23) can be changed.

The invention provides a device and a method for measuring a strain tensor of a two-dimensional material based on optical third harmonic generation. The device comprises a pulsed laser light source, areflecting mirror, a first polarizer, a beam splitter mirror, a 1/2 wave plate, a lens, a measured two-dimensional sample, a light filter, a second polarizer and a spectrograph. The device realizes the measurement of the strain tensor of the two-dimensional material, and has the characteristics of high measurement speed, simplicity and effectiveness, and no damage to the measured sample. The strain tensor measurement which is not limited by a two-dimensional material system (an energy band structure, crystal symmetry and the like) is realized for the first time; and a great help is provided for accurately regulating and controlling the performance of the two-dimensional material in the strain engineering to meet the requirements of application of the two-dimensional material to the fieldsof optics, electrics, optoelectronic devices and the like.