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In-situ detection device for chlorophyll concentration of water and detection method of in-situ detection device

A technology for in-situ detection of chlorophyll concentration, applied in measuring devices, material analysis through optical means, instruments, etc., can solve the problems of low accuracy and sensitivity of fluorescence detection, non-adjustable excitation optical path, high noise background, etc., to achieve effective Conducive to miniaturization and integration of instruments, low power consumption, and good light transmission

Active Publication Date: 2016-11-09
NO 49 INST CHINESE ELECTRONICS SCI & TECH GRP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide an in-situ detection device and detection method for chlorophyll concentration in water bodies; to solve the problem that existing devices adopt 180° or 90° optical path design, the excitation light path cannot be adjusted, and the generated fluorescence intensity is weak
When there are many impurities in the actual water body, scattering will occur, resulting in high noise background, low accuracy and sensitivity of fluorescence detection

Method used

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  • In-situ detection device for chlorophyll concentration of water and detection method of in-situ detection device
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  • In-situ detection device for chlorophyll concentration of water and detection method of in-situ detection device

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specific Embodiment approach 1

[0029] Specific implementation mode one: the following combination figure 1 and 2 This embodiment will be specifically described. An in-situ detection device for chlorophyll concentration in a water body according to this embodiment includes a conduit 1, a stainless steel casing 2, a detector 3, a quartz water sample chamber 4, a light source lateral adjustment support 5, a light source lateral adjustment mechanism 6, and a light source incident angle adjustment mechanism 7. Light source 8, water inlet filter screen 9, air duct 10, water sample chamber water inlet device 13, water inlet device positioning ring 14, water inlet restrictor plate 15, fixed partition 16, optical filter 18, focusing lens 19 And lens fixing device 20; The horizontal section of described quartz water sample chamber 4 is regular hexagonal structure;

[0030] The outside of the quartz water sample chamber 4 is provided with a stainless steel casing 2, the outer wall of the quartz water sample chamber ...

specific Embodiment approach 2

[0045] Embodiment 2: This embodiment differs from Embodiment 1 in that the optical filter 18 is a bandpass filter with a center wavelength of 680 nm and a half-maximum width of 10 nm. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0046] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: when the outer wall of the quartz water sample chamber 4 is sealed with the stainless steel shell 2 to form the cavity 17, 504 glue is used for connection and sealing. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to an in-situ detection device for the chlorophyll concentration of water and a detection method of the in-situ detection device. The in-situ detection device and the detection method aim at solving the problems that in an existing device, the excitation light path can be not adjusted, the fluorescence intensity is low, and the fluorescence detection accuracy and sensitivity are low due to scattering generated when water contains lots of impurities. A water sample chamber water inlet device is arranged at the bottom of a quartz water sample chamber, a stainless steel shell is arranged outside the quartz water sample chamber, a detector, a light filter and a focusing lens are arranged over the quartz water sample chamber, a light source, a light source transverse adjustment support, a light source transverse adjustment mechanism and a light source direction adjustment mechanism are arranged on the side face of the quartz water sample chamber, and the detector, the light source, the light source transverse adjustment mechanism and a light source incidence angle adjustment mechanism are connected with a host through wires. The method includes the steps that water background fluorescence signals measured under the off-state of the excitation light source are collected, then water fluorescence signals under the on-state of the excitation light source are collected, and background fluorescence interference is automatically subtracted through operation.

Description

technical field [0001] The invention relates to a chlorophyll concentration detection device and a detection method thereof. Background technique [0002] At present, there are three main detection methods of chlorophyll in water: spectrophotometry, high performance liquid chromatography and fluorescence analysis. The detection of spectrophotometry and high performance liquid chromatography requires sampling and filtration, 90% acetone grinding and extraction, and centrifugation to take the supernatant for testing. In order to prevent the sample from being acidified and destroying the pigment, magnesium carbonate needs to be added to it. The process is cumbersome and relies on large equipment. It is only suitable for laboratory testing, not suitable for field operations and real-time monitoring. At present, most of the existing fluorescence analysis chlorophyll detection devices use a fixed blue LED as the excitation light source to excite chlorophyll to generate 685nm fluo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6402
Inventor 夏露丁文波陈丽洁金建东王俊巍程鑫吴亚林王晓光王成杨
Owner NO 49 INST CHINESE ELECTRONICS SCI & TECH GRP
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