A kind of online evaluation method of cellulosic production Acetobacter xylinum cell concentration
Acetobacter xylinum, bacteria concentration technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, material capacitance, etc., can solve the problems of interfering light transmission, too large deviation of detection results, and bacterial contamination, etc., to improve Bacterial concentration, shortening the culture cycle, and reducing the effect of entanglement
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Embodiment 1
[0038] (1) Strain activation
[0039] Preparation of solid medium: glucose 2%-3%, polypeptone 0.5%-1%, citric acid 0.1%-0.2%, magnesium sulfate heptahydrate 0.03%-0.05%, ammonium sulfate 0.5%-1%, diphosphate Potassium hydrogen 0.3%-0.5%, sodium acetate 0.1%-0.2%, agar powder 2%-2.5%.
[0040] The freeze-dried preserved Acetobacter xylinum was spread on the plate solid medium, and cultured at 28°C for 4 days to form a single colony. Select a single colony cultivated above, spread it evenly on the solid medium on the slope with an inoculation loop, and cultivate it in a constant temperature incubator at 28°C for 4 days.
[0041] (2) shake flask culture
[0042] Preparation of liquid medium: 2%-3% glucose, 0.5%-1% yeast powder, 0.115%-0.2% citric acid, 0.34%-0.5% sodium hydrogen phosphate dodecahydrate, 0.5%-1% soybean peptone, Diammonium hydrogen phosphate 0.1%-0.2%.
[0043]Use a sterile spatula to take out the cultured slant film, put it into the shake flask liquid culture...
Embodiment 2
[0053] (1) Strain activation
[0054] Preparation of solid medium: glucose 2%-3%, polypeptone 0.5%-1%, citric acid 0.1%-0.2%, magnesium sulfate heptahydrate 0.03%-0.05%, ammonium sulfate 0.5%-1%, diphosphate Potassium hydrogen 0.3%-0.5%, sodium acetate 0.1%-0.2%, agar powder 2%-2.5%.
[0055] The freeze-dried preserved Acetobacter xylinum was spread on the plate solid medium, and cultured at 30°C for 6 days to form a single colony. Select the single colony cultured above, spread it evenly on the solid medium on the slope with an inoculation loop, and cultivate it in a constant temperature incubator at 30°C for 3 days.
[0056] (2) shake flask culture
[0057] Preparation of liquid medium: 1%-4% fructose, 0.5%-2% corn steep liquor, 0.2%-0.5% citric acid, 0.2%-0.5% sodium hydrogen phosphate dodecahydrate, 0.03%-0.05 magnesium sulfate heptahydrate %, diammonium hydrogen phosphate 0.4%-1%.
[0058] Use a sterile spatula to take out the cultured slant film, insert it into the s...
Embodiment 3
[0068] (1) Strain activation
[0069] Preparation of solid medium: glucose 2%-3%, polypeptone 0.5%-1%, citric acid 0.1%-0.2%, magnesium sulfate heptahydrate 0.03%-0.05%, ammonium sulfate 0.5%-1%, diphosphate Potassium hydrogen 0.3%-0.5%, sodium acetate 0.1%-0.2%, agar powder 2%-2.5%.
[0070] The freeze-dried and preserved Acetobacter xylinum was spread on the plate solid medium, and cultured at 32°C for 8 days to form a single colony. Select a single colony cultivated above, spread it evenly on the solid medium on the slope with an inoculation loop, and cultivate it in a constant temperature incubator at 32°C for 2 days.
[0071] (2) shake flask culture
[0072] Preparation of liquid medium: glucose 2-3%, corn steep liquor dry powder 0.5%-1.5%, citric acid 0.1%-0.3%, sodium hydrogen phosphate dodecahydrate 0.1%-0.3%, magnesium sulfate heptahydrate 0.02%-0.04% , Diammonium hydrogen phosphate 0.2%-0.6%.
[0073] Use a sterile spatula to take out the cultured slant film, ins...
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