168 results about "Exonuclease I" patented technology

The present invention provides a method and apparatus for automated DNA sequencing. The method of the invention includes the steps of: a) using a processive exonuclease to cleave from a single DNA strand the next available single nucleotide on the strand; b) transporting the single nucleotide away from the DNA strand; c) incorporating the single nucleotide in a fluorescence-enhancing matrix; d) irradiating the single nucleotide to cause it to fluoresce; e) detecting the fluorescence; f) identifying the single nucleotide by its fluorescence; and g) repeating steps a) to f) indefinitely (e.g., until the DNA strand is fully cleaved or until a desired length of the DNA is sequenced). The apparatus of the invention includes a cleaving station for the extraction of DNA from cells and the separation of single nucleotides from the DNA; a transport system to separate the single nucleotide from the DNA and incorporate the single nucleotide in a fluorescence-enhancing matrix; and a detection station for the irradiation, detection and identification of the single nucleotides. The nucleotides are advantageously detected by irradiating the nucleotides with a laser to stimulate their natural fluorescence, detecting the fluorescence spectrum and matching the detected spectrum with that previously recorded for the four nucleotides in order to identify the specific nucleotide.

Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified.

Sequencing methods that use an exonuclease that comprises template dependent nucleobase binding activity are provided. Related compositions and sequencing systems are also provided.

The invention discloses a sequencing library building method and reagent based on a molecular inverse probe. The method includes: using the molecular inverse probe and denatured nucleic acid to perform annealing hybridization, wherein the molecular inverse probe comprises the anchoring sequence of a 5' end, the extension sequence of a 3' end and the sequencing connector sequence between the anchoring sequence and the extension sequence, and the anchoring sequence and the extension sequence are respectively inversely complementary with sequences at two ends of a target area; using the target area as a template, and performing polymerization reaction starting from the extension sequence of the 3' end to generate the supplementary sequence of the target area; connecting the anchoring sequence of the 5' end with the supplementary sequence of the target area to form cyclic annular nucleic acid molecules; using exonuclease to digest non-cyclized linear molecules to obtain single-chain cyclic annular molecules containing the target area; sequencing the obtained single-chain cyclic annular molecules so as to achieve capture sequencing of the target area. The sequencing library building method has the advantages that the building of a single-chain cyclic annular library and target area capture are integrated, and library building process and cycle are shortened greatly.

Methods and compositions are provided for detecting DNA sequences, particularly single nucleotide polymorphisms, using a pair of nucleotide sequences, a primer and a snp detection sequence, where the snp detection sequence binds downstream from the primer to the target DNA in the direction of primer extension. The snp detection sequence is characterized by having a nucleotide complementary to the snp and adjacent nucleotides complementary to adjacent nucleotides in the target and an electrophoretic tag bonded to the 5'-nucleotide. The pair of sequences is combined with the target DNA under primer extension conditions, where the polymerase has 5'-3' exonuclease activity. When the snp is present, the electrophoretic tag is released and can be detected by electrophoresis as indicative of the presence of the snp in the target DNA. The sequence containing the snp is exemplary of DNA sequences of interest generally.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

Provided are DNA microarray techniques that allow hybridization without RNA amplification, without using cDNA, and without labeling the nucleic acid prior to hybridization. Referred to as the Double-stranded Exonuclease Protection (DEP) assay, the technique permits the sample RNA to be used directly for hybridization, without manipulation in any way. Further provided is a microarray technique for high-throughput miRNA gene expression analyses, termed the RNA-primed, Array-based, Klenow Enzyme (RAKE) assay. The RAKE assay is a sensitive and specific technique for assessing single-stranded DNA and RNA targets, and offers specific advantages over Northern blots.

A process for preparing pectose from witloof by hydrolysis features that the acidolsis is used to prepare the high-pectose syrup, the inulase is used to prepare chicorose mixture (PPE), the inulin endonuclease is used to prepare oligopectose by fermenting, and the inulin exonuclease is used to prepare the ultrahigh-pectose syrup by fermentation.

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.

The invention provides a method for identification of alleles. In this method, genomic DNA is used as target. Multiple allele-specific PCR amplification are carried out with a group of primers comprising one or more allele-specific primers for a target gene, a universal primer, and a common primer; and a DNA polymerase without 5′ to 3′ exonuclease activity. The PCR products are hybridized with tag probes immobilized on a DNA chip. Results are determined based on the signal intensity and the position of the probe immobilized on the array. Each allele-specific primer comprises a unique tag sequence at the 5′ end. Each tag probe immobilized on the DNA chip comprises a sequence identical to its corresponding tag sequence; and each tag probe hybridizes only with the complementary sequence in the PCR amplification product.

The invention provides a novel array method for nucleic acid sequence detection with improved specificity which allows for detection of genetic variation, from simple SNPs (where the variation occurs at a fixed position and is of limited allelic number) to more complex sequence variation patterns (such as with multigene families or multiple genetic strains of an organism where the sequence variation between the individual members is neither fixed nor consistent). The array is comprised of short, synthetic oligonucleotide probes attached to a solid surface which are hybridized to single-stranded targets. Single stranded targets can be produced using a method that employs primers modified on the 5′ end to prohibit degradation by a 5′-exonuclease that is introduced to degrade the unprotected strand. The invention further provides for printing buffers/solutions for the immobilization of oligonucleotide probes to an array surface. The invention also provides hybridization and wash buffers and conditions to maximize hybridization specificity and signal intensity, and reduce hybridization times.

The invention discloses a multi-RCA (rolling circle amplification) method based on split padlock probes. Each novel split padlock probe is about 90bp in length and comprises four parts, namely a detection arm, a universal primer area, an HhaI endonuclease site and a tag sequence area, wherein an amplification system is composed of a connection system and an RCA system. The concrete detection method comprises the steps of: firstly, carrying out coupled reaction, mixing a target sequence DNA (deoxyribonucleic acid) segment and four split padlock probes of which the final concentration is 1mol/L, hybridizing for 15 minutes after boiling and degenerating, adding T4DNA ligase and T4DNA ligase buffer solution, supplying 10 muL of reaction system; carrying out exonuclease I and exonuclease III exterior contact for 45 minutes at 37 DEG C, preparing an annular template and then carrying out RCA reaction; mixing, boiling and degenerating 10 muL of connection product and universal primer, respectively adding dNTP, phi29DNA polymerase, HhaI restriction enzyme and buffer solution to form 20 muL of reaction system, stewing for 60 minutes at 37 DEG C; finally detecting single-chain DNA product of RCA with a specific tag sequence, thus obtaining a corresponding test conclusion according to the result.

The invention discloses an African swine fever virus fluorescent type RAA detecting kit which comprises specific primers ASFV-F and ASFV-R and an RAA-exo probe ASFV-P. The basic groups in a target amplification subsequence are substituted by tetrahydrofuran (THF residues), dT-fluorophore and dT-quenching groups, a marked reported fluorescent dye is FAM, a fluorescent quenching group is BHQ-1, splitting decomposition can be conducted on the probe at the THF site by exonuclease in an agent, the fluorescent group can be separated from the quenching groups, and fluorescent signals are generated. By means of the kit, at the constant temperature of 37-42 DEG C, the African swine fever virus is detected within 20 minutes, the specificity is 100%, and the detection sensitivity is 10 copies per microliter. Thus, the kit has the advantages of being simple, rapid and sensitive in operation and provides effective technological means for on-site rapid detecting and screening of the African swine fever virus.

A process of amplifying a nucleic acid template dependent on partial destruction of primer molecules which have extended onto the template molecule followed by strand invasion of the partially destroyed primer template by a replacement primer. The destruction of the primer molecule may be performed by either endonuclease or exonuclease digestion. A signal generation from the amplified products may be obtained by the use of adaptors capable of binding probe molecules as well as the amplified product.

The invention discloses a multicolor fluorescence composite detection kit and detection method of 44 SNPs (Single Nucleotide Polymorphism) loci, which is used for individual recognition. The kit comprises a PCR (Polymerase Chain Reaction) primer, a DNA polymerase, a PCR buffer solution, an exonuclease I, a shrimp alkaline phosphatase, an extended primer and a SNaPshot reaction mixed solution; thePCR primer and the extended primer consist of primers containing 44 SNP gene loci and respectively aim to DNA fragments containing the 44 SNP loci. By adopting the invention, the genotyping of the 44SNP loci can be obtained quickly and accurately, and the individual recognition is carried out on human biological samples through detection and analysis of a fluorescence automatic typing apparatus.

The invention provides a method for detecting mercury ions in a solution, and a kit. The method comprises: 1) mixing the solution with single-stranded DNA containing thymine to form a mixture so that the single-stranded DNA containing the thymine forms double-stranded DNA through T-Hg2+-T base pairs wherein at least one deoxyribonucleotide of the single-stranded DNA containing the thymine is connected with a signal generating molecule; 2) contacting the double-stranded DNA obtained from the step 1) with exonuclease specifically different from the double-stranded DNA so that the exonuclease cuts the double-stranded DNA to release the deoxyribonucleotides connected with the signal generating molecules; and 3) detecting the signal generating molecules connected with the deoxyribonucleotides released in the detection step 2). The method has high specificity and sensitivity, does not need fixed DNA, is convenient to operate and can realize rapid detection.

The invention discloses a colorimetric method for detecting tobramycin based on double strand displacement and a three-dimensional DNA structure, and belongs to the field of food safety, medical analysis and environmental pollution detection. The method comprises the following steps: firstly, double strands T1/T2 are designed; when tobramycin exists, Bsm DNA polymerase synthesizes double strands which are completely complementary through a strong strand displacement reaction, and Nt.BstNBI incision endonuclease cuts recognition sites on the double strands; the three-way DNA structure capture reporter probes, and regenerates and replaces a large number of S1 strands containing G-quadruplex forming sequences. Thereafter, the G-quadruplex/heme catalyzes ABTS<2->/H2O2 chromogenic reaction, andthe tobramycin content can be determined by using the linear relationship between light absorption value and tobramycin concentration. According to the invention, an aptamer captures tobramycin to trigger double strand displacement reaction which is mediated by the Nt.BstNBI incision endonuclease and the Bsm DNA polymerase so as to generate a large number of reporter probes. Meanwhile, the reporter probes trigger lambda exonuclease-assisted loop amplification, so that multiple amplifications of colorimetric signals are realized, the detection range is widened, and the detection sensitivity isimproved.