61 results about "MiRNA Gene" patented technology

Medulloblastoma (MB) is the most common malignant brain tumor of children. To identify the genetic alterations in this tumor type, we searched for copy number alterations using high density microarrays and sequenced all known protein-coding genes and miRNA genes using Sanger sequencing. We found that, on average, each tumor had 11 gene alterations, markedly fewer than in common adult cancers. In addition to alterations in the Hedgehog and Wnt pathways, our analysis led to the discovery of genes not previously known to be altered in MBs. Most notably, inactivating mutations of the histone H3K4 trimethylase genes MLL2 or MLL3 were identified in 16% of MB patients. These results demonstrate key differences between the genetic landscapes of adult and childhood cancers, highlight dysregulation of developmental pathways as an important mechanism underlying MBs, and identify a role for a specific type of histone methylation in human tumorigenesis.

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA1318 genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA1318 genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA1318 gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA1318 gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

The invention discloses a method for early and accurate prediction of rice bacterial blight by using miRNA162b gene, and belongs to the field of biotechnology. The method comprises the following steps: selecting the rice to be tested and the control rice, wherein the selected control rice is not infected with bacterial blight but cultivated under the same conditions as the rice to be tested; isolating the rice to be tested and the rice to be tested respectively The total miRNA gene in the control rice; respectively detect the expression level of the miRNA162b gene in the total miRNA gene in the rice to be tested and the control rice, the sequence of the miRNA162b gene is as SEQ? ID? Shown in NO: 1; According to the expression levels of the miRNA162b gene in the rice to be tested and the control rice, judge whether the experiment is successful, and if successful, further determine whether the plant to be tested is infected with bacterial blight. The prediction method provided by the invention is successful, and can realize accurate forecast several days before the rice bacterial blight occurs, gaining time for early prevention and early treatment, and reducing the loss caused by the bacterial blight to rice.

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA172a genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA172a genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA172a gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA172a gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

The invention discloses an identification method for rice bacterial blight resistance, belonging to the field of biotechnology. The method comprises: inoculating the rice to be tested and the susceptible rice; isolating the miRNA gene in the rice to be tested and the susceptible rice; detecting the expression of the miRNA1425 gene locus; and judging whether the plant to be tested is resistant to bacterial blight. The application of the miRNA1425 gene site in identifying rice bacterial blight resistance. The present invention uses the transcription of the miRNA1425 gene locus for the first time to identify whether rice has bacterial blight resistance, and has achieved success. The transcription of the miRNA1425 gene locus provided by the invention is based on the detection of the expression level, avoiding the detection caused by white leaf blight. The misjudgment caused by the silencing of the resistant gene of blight, the identification method of the present invention does not utilize the conventional DNA linkage marking method, avoids the misjudgment caused by the loose DNA linkage, improves the accuracy of detection, and avoids unnecessary Production loss.

The invention provides a micro-Ribose Nucleic Acid (miRNA) for regulating and controlling the expression of a rustproofing bacterium infected gene of hybrid poplar and use of the miRNA. The miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar and the use of the miRNA have important biological significance and potential application value in poplar disease resistance breeding by virtue of study on the miRNA and the target gene thereof. The nucleotide sequence of the miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar is as shown in SEQ ID NO: 1. The precursor sequence of the miRNA is as shown in SEQ ID NO: 2. The miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar is capable of improving the resistance of the hybrid poplar to the rust disease and is applied to disease resistance breeding. The study on the miRNA by use of genetic engineering methods is capable of laying a foundation for intensively researching and controlling the rust disease infection resistance mechanism of the poplar and improving the resistance of the hybrid poplar to the rust disease.