Identification method of rice bacterial leaf blight resistance

A technology for bacterial blight resistance and identification method, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as inaccurate resistance to bacterial blight, and achieves that it is not easy to degrade and avoid misjudgment. , the effect of improving the accuracy

Active Publication Date: 2014-08-06
JIANGHAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] In order to solve the shortcomings of using DNA molecular markers to identify whether rice is resistant to bacterial blight is not accurate enough, the embodiment of the present invention provides a method for identifying rice bacterial blight resistance

Method used

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  • Identification method of rice bacterial leaf blight resistance
  • Identification method of rice bacterial leaf blight resistance

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Embodiment

[0025] The present invention mixes and hybridizes the miRNA gene of rice with the probe labeled miRNA1425 gene, detects the result by non-denaturing polyacrylamide gel electrophoresis, compares the results of the rice to be tested and the susceptible rice, and then judges the To test whether rice is resistant to bacterial blight, the specific experimental process is as follows.

[0026] Susceptible rice: select non-bacterial blight-resistant rice seeds labeled 9311 with normal growth and no obvious pests and diseases on the surface.

[0027] Rice to be tested: select rice seeds with the label 9311 resistant to bacterial blight.

[0028] Among them, rice seeds resistant to bacterial blight have resistance gene Xa23.

[0029] Pre-treatment of seeds: Soak in 70% alcohol for 2 minutes, then wash twice with deionized water, soak in water at 30°C overnight, and germinate the seeds at a temperature of 30°C. After the buds grow out, sow In the seedling field, the management of the s...

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Abstract

The invention discloses an identification method for rice bacterial blight resistance, belonging to the field of biotechnology. The method comprises: inoculating the rice to be tested and the susceptible rice; isolating the miRNA gene in the rice to be tested and the susceptible rice; detecting the expression of the miRNA1425 gene locus; and judging whether the plant to be tested is resistant to bacterial blight. The application of the miRNA1425 gene site in identifying rice bacterial blight resistance. The present invention uses the transcription of the miRNA1425 gene locus for the first time to identify whether rice has bacterial blight resistance, and has achieved success. The transcription of the miRNA1425 gene locus provided by the invention is based on the detection of the expression level, avoiding the detection caused by white leaf blight. The misjudgment caused by the silencing of the resistant gene of blight, the identification method of the present invention does not utilize the conventional DNA linkage marking method, avoids the misjudgment caused by the loose DNA linkage, improves the accuracy of detection, and avoids unnecessary Production loss.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for identifying rice bacterial blight resistance. Background technique [0002] Rice is the most important food crop in my country. Bacterial blight is one of the two major diseases of rice. It is a worldwide disease, especially in Asian rice regions. The incidence of bacterial blight is high and the infection is fast, and the yield of affected paddy fields will be reduced by 20-30%, or even 50%. [0003] Breeding and utilizing disease-resistant varieties is the most economical and effective means to solve the grain yield reduction caused by bacterial blight. Therefore, it is necessary to identify the resistance of cultivated rice to bacterial blight. At present, the method for identifying rice bacterial blight usually uses DNA (Deoxyribonucleic acid, deoxyribonucleic acid) molecular markers, and uses the principle of DNA linkage markers to identify whether rice has a bacte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 周俊飞彭海章伟雄张静
Owner JIANGHAN UNIVERSITY
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