55results about How to "Sequencing is facilitated" patented technology

A hydrocarbyldithiomethyl-modified compound of the Formula:

R¹—O—CH₂—S—S—R²

or a salt thereof wherein R¹ is an organic molecule and R² is a hydrocarbyl is useful for protecting and/or blocking hydroxyl groups in organic molecules such as nucleotides. The hydrocarbyldithiomethyl-modified compounds can also be used for chemically synthesizing oligonucleotides and for sequencing nucleic acid compounds.

An implantable multi-chamber cardiac stimulation device includes flexibly programmable electrode stimulation configurations, and is capable of precisely controlling the stimulation sequence between multiple sites. The stimulation device provides a plurality of connection ports that allow independent connection of each electrical lead associated with a particular stimulation site in the heart. Each connection port further provides a unique terminal for making electrical contact with only one electrode such that no two electrodes are required to be electrically coupled. Furthermore, each electrode, whether residing on a unipolar, bipolar or multipolar lead, may be selectively connected or disconnected through programmable switching circuitry that determines the electrode configurations to be used for sensing and for stimulating at each stimulation site. The stimulation device allows for the programmable selection of each electrode terminal connection to a relatively positive or negative battery potential. In this way, each electrode, when electrically connected, may be programmed to act as the cathode or as the anode during sensing or stimulation delivery. Thus, directionality of the depolarization wave may be controlled by programming the cathode and anode assignments of the stimulation electrodes.

Disclosed are mutant CDK inhibitor (CKI) polypeptides having dominant negative antagonist activity against wild-type CKI proteins, as well as related compositions, including nucleic acids and vectors encoding the mutant CKI polypeptides and transformed host cells and transgenic plants comprising such nucleic acids and vectors. Also disclosed are related methods for using the mutant proteins to modulate cell division in cells, particularly plant cells.

The present disclosure relates to compositions and methods of RNA analysis. In particular, the present disclosure provides a method of RNA analysis that includes obtaining a sample, applying one or more multi-partite probes to the sample, where each of the one or more multi-partite probes includes at least two sub-probes, annealing at least one of the applied one or more multi-partite probes to at least one target nucleic acid within the sample, and ligating the at least two sub-probes associated with the at least one annealed multi-partite probe to create a target nucleic acid proxy that can be detected.

A sole for a shoe with a foot guiding mechanism which has the particularity that the sole comprises a sole body which has, on an outer face thereof, at least one protrusion. The at least one protrusion is flexible in order to produce a desired movement of the protrusion which is suitable to force a guided sequence for a foot, wearing the shoe sole, from when the heel section initially contacts a ground surface to when the front edge of the sole breaks contact with the ground surface.

A method for selective labeling of phosphate groups in natural and synthetic oligomers and polymers in the presence of chemically related groups such as carboxylic acid groups. The method is specifically applicable to biological oligomers and polymers, including phosphopeptides, phosphoproteins and phospholipids. In a specific embodiment, selective labeling of phosphate groups in proteins and peptides, for example, facilitates separation, isolation and detection of phosphoproteins and phosphopeptides in complex mixtures of proteins. Selective labeling can be employed to selectively introduce phosphate labels at phosphate groups in an oligomer or polymer, e.g., in a peptide or protein. Detection of the presence of the label, is used to detect the presence of the phosphate group in the oligomer or polymer. The method is useful for the detection of phosphoproteins or phosphopeptides. The phosphate label can be a colorimetric label, a radiolabel, a fluorescent or phosphorescent label, an affinity label or a linker group carrying a reactive group (or latent reactive group) that allows selective attachment of the oligomer or polymer (protein or peptide) to a phosphate label, to an affinity label or to a solid support. The method can be combined with well-known methods of mass spectrometry to detect and identify phosphopeptides and phosphoproteins.

Therapeutic oligonucleotide analogues which have improved nuclease resistance and improved cellular uptake are provided. Replacement of the normal phosphorodiester inter-sugar linkages found in natural oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.

Novel compounds that mimic and/or modulate the activity of wild-type nucleic acids. In general, the compounds contain a selected nucleoside sequence wherein the nucleosides are covalently bound through linking groups that contain adjacent nitrogen atoms.

The present invention provides methods for sequencing and genotyping of DNA useful for analysis of samples in which the target DNA represents a small portion (e.g., 10-1000-fold less) that a contaminating DNA source. Accordingly, the methods described herein are useful for sequencing or genotyping pathogen DNA, such as malaria DNA, in clinical samples taken from infected subjects.

A method of playing an interactive card game for orchestrating clean up of a space and teaching organization and task-related skills. The card game includes a deck of cards composed of cards describing and depicting respective activities to be executed by at least one player and cards depicting rewards; and objects which facilitate playing the card game and which are at least one of present in the space and supplied with the card game. The card game is used for orchestrating clean up of the space and teaching organization including sorting and task-related skills by playing the card game which is presented in a fun and educational format so that players perceive the process of sorting and cleaning up as a pleasant experience.

An adhesive patch, such as poultice-type patch, is provided, which is suitable for mass production and allows the user to apply it without touching the drug-containing matrix. A production method of such a adhesive patch is also provided.

Specifically, the method for producing adhesive patch, wherein

(a) the adhesive patch comprises two liners;

(b) the first liner is folded at the middle thereof so that it is divided by the fold into two sections that together form a V-shaped liner, one of the two sections adhering to the drug-containing matrix surface; and

(c) the second liner adheres to the remaining part of the drug-containing matrix surface with one end of the second liner covering the fold of the V-shaped first liner, the method including:

allowing a tubular liner to adhere to at least half of the surface of the drug-containing matrix spread over the backing; and

cutting the tubular liner in half to form the V-shaped first liner folded at the middle thereof.

In a method and a pulse sequence determination device to determine a pulse sequence for a magnetic resonance system, control protocol parameter values are initially acquired. A determination of k-space trajectory node points within k-space then takes place in a processor on the basis of the control protocol parameter values. The determination of the pulse sequence then takes place on the basis of the k-space trajectory node points. A method for operating a magnetic resonance system uses such a pulse sequence, and a magnetic resonance system embodies such a pulse sequence determination device.

An adhesive patch is provided that allows the user to apply it in a safe and hygienic manner without touching the drug-containing matrix during the sequence of actions from the removal of the liner to the application of the patch. Specifically, the adhesive patch has a multilayer structure that includes a backing, an adhesive drug-containing matrix that is spread substantially entirely over one surface of the backing, and a liner that adheres to the drug-containing matrix surface. The adhesive patches have the following features:

(a) the liner adhering to the drug-containing matrix surface includes first and second liners;

(b) the first liner is folded at the middle thereof so that it is divided by the fold into first and second sections that together form a V-shaped liner, the first section adhering to the drug-containing matrix surface from one end of the matrix with the fold arranged closer to the middle of the drug-containing matrix, the second section serving as a tab; and

(c) the second liner adheres to the remaining part of the drug-containing matrix surface with one end of the second liner covering the fold of the V-shaped first liner to form a laminated part that serves as a tab.

Therapeutic oligonucleotide analogs which have improved nuclease resistance and improved cellular uptake are provided. Replacement of phosphorodiester inter-sugar linkages found in wild type oligomers with four atom linking groups forms unique di- and poly-nucleosides and nucleotides useful in regulating RNA expression and in therapeutics. Methods of synthesis and use are also disclosed.

The present invention provides methods for assembling DNA molecules in a predetermined order to produce a DNA construct useful in Agrobacterium mediated transformation in plants. The method employs ligation independent cloning of separate DNA elements where one of the DNA elements contains T-DNA borders from Agrobacterium tumefaciens. The invention further provides methods for assembling a construct containing an inverted repeat. Using this approach, DNA constructs are constructed rapidly, efficiently and directionally.

The present invention relates to a method for determining the sequence of a nucleic molecule. Herein a capture oligonucleotide probe is attached to an encoded microcarrier, wherein the code of said microcarrier identifies the sequence of said oligonucleotide probe. The capture oligonucleotide probe is hybridized with a sample comprising nucleic acids molecules, wherein said DNA fragment comprises a sequence which is complementary to the sequence of the capture oligonucleotide probe. The sequence of the DNA molecule is determined, wherein the capture oligonucleotide probe serves as a primer for a DNA polymerase, in the case of single molecule sequencing this is a sequencing primer. After the sequence determination, the nucleotide sequence of the capture oligonucleotide probe is identified by determining the code on the microcarrier, which corresponds with the capture oligonucleotide probe. This sequence information directly identifies the location of the sequenced DNA fragment on the genome, allowing direct comparison.

A sole for a shoe with a foot guiding mechanism which has the particularity that the sole comprises a sole body which has, on an outer face thereof, at least one protrusion. The at least one protrusion is flexible in order to produce a desired movement of the protrusion which is suitable to force a guided sequence for a foot, wearing the shoe sole, from when the heel section initially contacts a ground surface to when the front edge of the sole breaks contact with the ground surface.

Novel compounds that mimic and/or modulate the activity of wild-type nucleic acids. In general, the compounds contain a selected sequence of nucleosidic bases or other reactive groups that are covalently bound through nitrogen-containing linear, hairpin, dumbbell, and circular shaped tethers.