53 results about "Histone methylation" patented technology

The invention provides compositions and methods of use to enhance reprogramming of mammalian cells. Certain compositions and methods of the invention are of use to enhance generation of induced pluripotent stem cells by reprogramming somatic cells. Certain compositions and methods of the invention are of use to enhance reprogramming of pluripotent mammalian cells to a differentiated cell type. Certain compositions and methods of the invention are of use to enhance reprogramming of differentiated mammalian cells of a first cell type to differentiated mammalian cells of a second differentiated cell type. The reprogrammed somatic cells are useful for a number of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that enhances or contributes to reprogramming mammalian cells. Certain of the inventive compositions and methods relate to inhibiting histone methylation.

Methods, kits, and compositions for detecting the methylation status of various genes are useful in various diagnostic applications involving suspected proliferative disorders such as prostate cancer.

A population of enteroendocrine cells (EEC) is obtained from a mammalian post-natal cell population, such as a population including post-natal stem cells, by treating the population with a plurality of small molecules that upregulate ChgA and promote differentiation of the cells to form the enteroendocrine cells. The upregulation of ChgA is such that the fraction of cells expressing CGA in the obtained cell population, as measured by a ChgA Immunostaining Assay, is at least about 1.5%. Small molecules that can be used to differentiate the post-natal cells into the enteroendocrine cells can include at least one of a Wnt activator, a Notch inhibitor, a Wnt inhibitor, a MEK/ERK inhibitor, a growth factor, a HDAC inhibitor, a Histone Methylation Inhibitor, a Tgf-β inhibitor, and a NeuroD1 activator. Also, the insulin expression of a population of mammalian cells is increased by treating the population with a plurality of small molecules that increase the insulin expression.

The invention provides a multiple lung cancer-related gene methylation combined detection kit, which comprises: a detection reagent for specifically detecting the DNA methylation of human SHOX2 gene;a detection reagent for specifically detecting the DNA methylation of human RASSF1A gene; a detection reagent for specifically detecting the DNA methylation of human ANKRD18B gene; and a detection reagent for specifically detecting the DNA methylation of human MPDZ gene, wherein preferably the detection reagents are primers and probes. The invention further provides applications of the multiple lung cancer-related gene methylation combined detection kit in multiple lung cancer-related gene methylation combined detection, and a multiple lung cancer-related gene methylation combined detection method. According to the present invention, the multiple lung cancer-related gene methylation combined detection kit can perform the combined detection on the multiple lung cancer-related gene methylation, can significantly improve the tumor detection rate, has advantages of clever design and simple structure, and is suitable for large-scale promotion and applications.

The invention discloses an application of tri-methylation of histone H3lysine 4( H3K4me3) to granulosa cells of pigs, and belongs to the technical field of cell engineering and genetic engineering. H3K4me3 is used as a penetration point, and a cell biology method is used for researching the influence of the H3K4me3 to the functions of the granulosa cells of pigs. The technical scheme is detailed in design, and reliable in results. In order to confirm the influence of H3K4me3 to the functions of the granulosa cells of the pigs, verification is performed from multiple levels and multiple angles,and verification is performed from the cell level and the protein level. The invention explains that the H3K4me3 has influence on the functions of the granulosa cells of the pigs, the H3K4me3 can promote proliferation of the granulosa cells of the pigs, can restrain the apoptosis of the granulosa cells of the pigs, and can reduce the proportion of the granulosa cells of the pigs, blocked in the GO/G1 period. The application has a good application value of research of histone methylation on an influence mechanism on the development of granulosa cells and the reproductive performance of sows.

A pharmaceutical composition for induction therapy which has a hypomethylating agent and a histone deacetylase inhibitor (“HDAC inhibitor”); wherein the hypomethylating agent is a DNA and histone methylation inhibitor such as cladribine and the HDAC inhibitor is, for example, entinostat, panobinostat, vorinostat, and/or romedepsin; further wherein the hypomethylating agent and the HDAC inhibitor are combined in formulations for various administrations including e.g., a continuous delivery system such as a transdermal patch of at least one reservoir or a plurality of reservoirs, oral, a fixed-dose oral combination, intravenous, and combinations thereof. This pharmaceutical composition for induction therapy is used with a monoclonal antibody in the treatment of various cancers, sarcomas, and other malignancies.

It is intended to provide a method of conveniently and economically detecting the methylation of a gene contained in a biological sample. It is also intended to provide an accurate and sensitive examination method to be used in the case of examining a neoplasm by using the method of detecting gene methylation. In examining the methylation of a gene, a polysaccharide is added to a biological sample before treating it with a bisulfite. Thus, the subsequent bisulfite treatment can be conducted while requiring neither the addition of a protein-denaturing agent nor the purification of DNA. Thus methylated cytosine without uracil-modification can be detected. Moreover, a neoplasm can be examined at an elevated accuracy by detecting the methylation of genes of multiple species contained in a biological sample and calculating the sum thereof.

Described is a method for detecting 5-methylcytosine in genomic DNA samples. First, a genomic DNA from a DNA sample is chemically converted with a reagent, 5-methylcytosine and cytosine reacting differently, and the pretreated DNA is subsequently amplified using a polymerase and at least one primer. In the next step, the amplified genomic DNA is hybridized to at least two oligonucleotides, the latter being joined by inserting at least one oligonucleotide. In the ligation product, one nucleotide carries a detectable label, and the elongation depends on the methylation status of the specific cytosine in the genomic DNA sample. In the next step, the elongated oligonucleotides are analyzed for the presence of the label.

The invention discloses a vector and method for increasing cattle cloning efficiency based on histone methylation level modification. The vector comprises the in-vitro transcription template vector containing a cattle KDM4E gene. A large amount of mRNA of the cattle KDM4E gene can be obtained by subjecting the vector to in-vitro transcription reaction, and the mRNA is injected to a successfully fused and activated bovine somatic cell cloning embryo to allow the embryo to overexpress cattle KDM4E protein. The method has the advantages that histone H3K9me3 and H3K9me2 epigenetic modification level of the somatic cell cloning embryo during zygotic activation can be effectively lowered, so that the development rate and quality of later bovine somatic cell cloning embryo can be increased evidently, the method can be used for efficiently producing the bovine somatic cell cloning embryo in vitro, and the birth rate of cloning cattle after embryo transplanting is increased evidently.

The invention relates to a signal-enhanced test strip biosensor for detecting histone methylation. The test strip biosensor comprises a bottom plate with viscidity, a nitrocellulose membrane, a colloidal gold pad, an absorbent paper, and a sample pad, wherein the nitrocellulose membrane is attached to the middle part of the bottom plate; a monoclonal antibody of anti-histone H3 and a goat-anti-rat IgG antibody are sprayed on the nitrocellulose membrane; a detection line and a quality control line are respectively formed; the colloidal gold pad is attached to the bottom plate at one side of the nitrocellulose membrane; gold nanoparticle solution doubly marked by c-DNA (deoxyribonucleic acid) and the monoclonal antibody of anti-histone H3 is sprayed on the colloidal gold pad; the absorbent paper is attached to the bottom plate at the other side of the nitrocellulose membrane; the sample pad is attached to the bottom plate; histone extract is firstly dropwise added to the sample pad in detection; then an enhanced probe formed by DNA and gold nanoparticles in a coupling manner is dropwise added. By adopting the test strip biosensor provided by the invention, the histone methylation condition can be simply and rapidly detected; a detection signal is further amplified by the enhanced probe.

The invention provides a method for promoting in-vitro embryonic development by regulating metabolism, and relates to the technical field of biology. The inventor researches and finds that in the in-vitro embryo culture process, addition of L-2-hydroxyglutaric acid can hinder erasing of histone methylation and embryo development, and reduction of the content of L-2-hydroxyglutaric acid in an embryo can promote erasing of histone methylation and embryo development. Therefore, the invention provides the method for regulating in-vitro embryonic development of mammals, which comprises the step of promoting embryonic development by reducing the content of L-2-hydroxyglutaric acid in embryos, or inhibiting embryonic development by increasing the content of L-2-hydroxyglutaric acid in embryos. By adopting the method provided by the invention, regulation and control on in-vitro embryonic development can be effectively realized, and a new view is provided for a molecular regulation and control mechanism in an early embryonic development process.

The ASH2L expression would be specific decrease in the PMA exciting K562 differentiation process. And the DPY-30-like and WDR5 have no such alteration. Thus, ASH2L could be used as the alternate mark material to diagnose leukaemia. It constructs the N-end albumen of ASH2L and DPY-30-like full length albumen plasmid to transform to bacilluscoli expression corresponding albumen. It could be used to diagnose bacilluscoli diagnose.

The invention discloses a gene 03g induced to express by tissue culture in rice and an application. Through a known public database, genes specifically expressed by callus are sieved and the gene 03g after callus induction is expressed continuously, that is, after callus differentiation, 03g is still expressed in high quantity when other genes lose expression activity. Through hybridization of 0.3g expressed plant with wild plant, expression of the 03g is not affected by the other parent. Therefore, expression or no expression of 03g or not can be used to detect whether the rice plant is transgenetic or not. Molecular biological experiments show that expression of 03g is regulated by DNA (Deoxyribonucleic Acid) methylation and histone methylation. Expression of 03g provided by the invention is epigenetically regulated, and the expression mode can be applied to detecting transgenetic rice.

Described is a method for detecting 5-methylcytosine in genomic DNA samples. First, a genomic DNA from a DNA sample is chemically converted with a reagent, 5-methylcytosine and cytosine reacting differently, and the pretreated DNA is subsequently amplified using a polymerase and at least one primer. In the next step, the amplified genomic DNA is hybridized to at least one oligonucleotide, forming a duplex, and said oligonucleotide is elongated by at least one nucleotide, the nucleotide carrying a detectable label, and the elongation depending on the methylation status of the specific cytosine in the genomic DNA sample. In the next step, the elongated oligonucleotides are analyzed for the presence of the label.

The invention discloses an application of histone methylation H3K4me3 in the mouse ovarian development. The effect of H3K4me3 on the mouse ovarian follicle development is studied from the living bodylevel and protein level, and the result confirms that the H3K4me3 degree increase can promote the mouse ovarian follicle development, inhibit the mouse follicle apoptosis, and increase the litter sizeand litter weight of mice; and the H3K4me3 degree lowering before sexual maturity in mice can inhibit the secretion of GnRH and gonadotropin-releasing hormone LH and FSH in the blood of the mice. A foundation for further exploring the mechanism of H3K4me3 targeting and regulating key genes of the mouse ovarian follicle development at the molecular level, and then regulating the mechanism of the ovarian follicle development is laid.

The invention relates to trichoderma reesei engineering bacteria for high yield of sorbicillinoids and a construction method and application of the trichoderma reesei engineering bacteria. The trichoderma reesei engineering bacteria disclosed by the invention are constructed by knocking out genes Trclr4 of trichoderma reesei with trichoderma reesei QM9414 as an original strain. By knocking out thecoding gene Trclr4 of histone H3K9 methylase of the trichoderma reesei, the important role of histone H3K9 methylation in synthesis of sorbicillinoids from the trichoderma reesei is proved for the first time, and high yield of the sorbicillinoids in the trichoderma reesei is realized. Compared with the original strain, the trichoderma reesei delta Trclr4 engineering bacteria have the advantage that the yield of the sorbicillinoids under the condition of taking cellulose as a carbon source is doubled.