Kit for detecting PAX1 (Paired Box 1) gene methylation in cervical cancer
A kit and technology for cervical cancer, applied in the field of kits for detection of cervical cancer PAX1 gene methylation, can solve the problems of complex operation, cross-contamination of external environment and detection reagents, and inability to integrate, and achieve accurate results
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Embodiment 1
[0098] This embodiment provides a kit for detecting the methylation of cervical cancer PAX1 gene, such as Figure 1-7 As shown, it includes an extraction chamber 1 , a cracking mechanism 2 , a washing mechanism 3 , an eluting mechanism 4 , a driving mechanism 5 , an amplification tube 6 , an upper cover 7 , a bracket 8 and a bottom cover 9 .
[0099] Wherein, the extraction chamber 1 is made of elastic material, which includes a cracking mechanism 2, a washing mechanism 3, an eluting mechanism 4, a buffer tank 101 and an elastic sealing film 104 distributed sequentially according to the sample flow direction; the bracket 8 is fixed on the extraction chamber 1 , the two are bonded by an adhesive; the upper cover 7 and the bottom cover 9 are detachably connected, the two surround a cavity, and the extraction bin 1 and the bracket 8 are located in the cavity; the bracket 8 is provided with an upper cover installation hole 801 , for connecting with the upper cover 7.
[0100] The...
Embodiment 2
[0151] This embodiment provides a kit for detecting the methylation of cervical cancer PAX1 gene, such as Figure 8-14 As shown, it includes an extraction chamber 21 , a cracking mechanism 22 , a washing mechanism 23 , an elution mechanism 24 , a driving mechanism 25 , an amplification tube 26 and a top cover 27 .
[0152] Wherein, the extraction chamber 21 includes a lysing mechanism 22 , a washing mechanism 23 , an elution mechanism 24 and a buffer tank 2101 distributed sequentially according to the sample flow direction.
[0153] The lysing mechanism 22 is provided with a lysing reagent and a magnetic substance; the magnetic substance is a magnetic bead.
[0154] The washing mechanism 23 includes a first oil tank 2301 and a washing tank 2302 , and washing liquid is provided in the washing tank 2302 ; the first oil tank 2301 is located between the cracking mechanism 22 and the washing tank 2302 .
[0155] The elution mechanism 24 comprises a second oil tank 2401 and an elut...
Embodiment 3
[0169] The difference between this embodiment and Embodiment 1 is that no amplification tube is provided, and the amplification reaction is carried out in the buffer tank; at this time, there is no need to set a connector, and a discharge port is directly set on the buffer tank for amplification. Increase the outflow of the sample after the reaction.
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