Kit for detecting PAX1 (Paired Box 1) gene methylation in cervical cancer

A kit and technology for cervical cancer, applied in the field of kits for detection of cervical cancer PAX1 gene methylation, can solve the problems of complex operation, cross-contamination of external environment and detection reagents, and inability to integrate, and achieve accurate results

Active Publication Date: 2018-11-06
GUANGZHOU HEAS BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The detection process is cumbersome, and many links need to be manually operated; nucleic acid extraction, nucleic acid sulfite conversion, and nucleic acid methylation detection are divided into three parts, which cannot be performed at the same time. There are many steps and complicated operations; nucleic acid extraction and nucleic acid sulfite conversion cannot be combined. The nucleic acid methylation detection reagents are integrated, and the detection process is open, which is easy to cause cross-contamination between the external environment and the detection reagents; the detection time is long

Method used

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  • Kit for detecting PAX1 (Paired Box 1) gene methylation in cervical cancer
  • Kit for detecting PAX1 (Paired Box 1) gene methylation in cervical cancer
  • Kit for detecting PAX1 (Paired Box 1) gene methylation in cervical cancer

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Experimental program
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Effect test

Embodiment 1

[0098] This embodiment provides a kit for detecting the methylation of cervical cancer PAX1 gene, such as Figure 1-7 As shown, it includes an extraction chamber 1 , a cracking mechanism 2 , a washing mechanism 3 , an eluting mechanism 4 , a driving mechanism 5 , an amplification tube 6 , an upper cover 7 , a bracket 8 and a bottom cover 9 .

[0099] Wherein, the extraction chamber 1 is made of elastic material, which includes a cracking mechanism 2, a washing mechanism 3, an eluting mechanism 4, a buffer tank 101 and an elastic sealing film 104 distributed sequentially according to the sample flow direction; the bracket 8 is fixed on the extraction chamber 1 , the two are bonded by an adhesive; the upper cover 7 and the bottom cover 9 are detachably connected, the two surround a cavity, and the extraction bin 1 and the bracket 8 are located in the cavity; the bracket 8 is provided with an upper cover installation hole 801 , for connecting with the upper cover 7.

[0100] The...

Embodiment 2

[0151] This embodiment provides a kit for detecting the methylation of cervical cancer PAX1 gene, such as Figure 8-14 As shown, it includes an extraction chamber 21 , a cracking mechanism 22 , a washing mechanism 23 , an elution mechanism 24 , a driving mechanism 25 , an amplification tube 26 and a top cover 27 .

[0152] Wherein, the extraction chamber 21 includes a lysing mechanism 22 , a washing mechanism 23 , an elution mechanism 24 and a buffer tank 2101 distributed sequentially according to the sample flow direction.

[0153] The lysing mechanism 22 is provided with a lysing reagent and a magnetic substance; the magnetic substance is a magnetic bead.

[0154] The washing mechanism 23 includes a first oil tank 2301 and a washing tank 2302 , and washing liquid is provided in the washing tank 2302 ; the first oil tank 2301 is located between the cracking mechanism 22 and the washing tank 2302 .

[0155] The elution mechanism 24 comprises a second oil tank 2401 and an elut...

Embodiment 3

[0169] The difference between this embodiment and Embodiment 1 is that no amplification tube is provided, and the amplification reaction is carried out in the buffer tank; at this time, there is no need to set a connector, and a discharge port is directly set on the buffer tank for amplification. Increase the outflow of the sample after the reaction.

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Abstract

The invention provides a kit for detecting PAX1 (Paired Box 1) gene methylation in cervical cancer. The kit comprises a splitting reagent and a binding fortifier, wherein the splitting reagent comprises a splitting modification solution which is prepared from ammonium hydrogen sulfite, ammonium sulfite, guanidine hydrochloride, sodium hydroxide and hydroquinone; the binding fortifier is prepared from isopropanol and sodium chloride. According to the kit provided by the invention, extraction and amplification detection of nucleic acid are integrated under a totally enclosed environment, high efficiency and simpleness are realized, and a result is accurate.

Description

technical field [0001] The invention relates to a kit for detecting the methylation of the cervical cancer PAX1 gene. Background technique [0002] The nucleic acid diagnostic analysis method based on PCR technology is the core technology in molecular diagnosis. Integrating the automatic nucleic acid extraction process with the PCR amplification detection process to construct an integrated nucleic acid extraction and amplification detection system has important practical significance for further improving the efficiency of nucleic acid diagnostic analysis and realizing the automation of the entire nucleic acid diagnostic analysis process. [0003] Chinese patent application 201610266982.8 discloses a cervical cancer cell detection kit based on boron-doped graphene quantum dots, which belongs to the field of biological detection. The cervical cancer cell detection kit includes reagents A, B and C, reagent A is a boron-doped graphene quantum dot solution, reagent B is a ceriu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12M1/34C12M1/00
CPCC12Q1/6886C12Q2600/154
Inventor 刘淑园陈华云张天海王盼盼
Owner GUANGZHOU HEAS BIOTECH CO LTD
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