Application of leucocythemia related to gene ASH2L in leukemia molecule classification diagnosis

A technology for molecular typing and leukemia, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of uncertain expression characteristics of ASH2L, limitations of ASH2L in molecular typing diagnosis, unclear expression characteristics, etc.
CN1869225AInactive Publication Date: 2006-11-29THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
THE INST OF BASIC MEDICAL SCI OF CHINESE ACAD OF MEDICAL SCI
Publication Date
2006-11-29
Estimated Expiration
Not applicable · inactive patent

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Abstract

The ASH2L expression would be specific decrease in the PMA exciting K562 differentiation process. And the DPY-30-like and WDR5 have no such alteration. Thus, ASH2L could be used as the alternate mark material to diagnose leukaemia. It constructs the N-end albumen of ASH2L and DPY-30-like full length albumen plasmid to transform to bacilluscoli expression corresponding albumen. It could be used to diagnose bacilluscoli diagnose.
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Description

technical field

[0001] The invention belongs to the fields of gene recombination, antibody preparation and clinical diagnosis. Specifically, the present invention relates to a recombinant genetic engineering and protein purification process of the N-terminal part of the leukemia-related gene ASH2L, to the recombinant genetic engineering and protein purification process of the full-length ASH2L interacting protein DPY-30-like, and to the use of The preparation of highly specific antibodies from these two purified proteins involves the specific downregulation of ASH2L during the differentiation process of K562 cells stimulated by PMA, the recombinant plasmids containing part of the ASH2L gene and the recombinant strains expressing the protein and their use in the molecular typing diagnosis of leukemia Applications. Background technique

[0002] Accurate classification of leukemia is an important prerequisite for the treatment of leukemia. At present, the diagnosis and typing...

Claims

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