Method for studying mechanism of combined effect of Wnt signal and histone on target gene during differentiation of PGCs
A histone and target gene technology, applied in biochemical equipment and methods, genetic engineering, plant gene improvement, etc., can solve the problem of lack of Wnt signaling pathway and epigenetic factors to coordinate the regulation of target gene transcription.
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[0163] The inventor's research shows that the target gene of Wnt / β-catenin / Tcf7l2 is Lin28A / B, based on this, the participation of histone methylation in this regulation process is studied.
[0164] 1. Research on the regulation of Lin28A / B transcription by H3K4me2
[0165] (1) In vitro verification that H3K4me2 positively regulates Lin28A / B transcription
[0166] The inventors have shown that the histone methylase MLL2 (also known as Kmt2d) and the histone demethylase LSD1 can regulate the methylation status of H3K4me2, respectively using the constructed MLL2 and LSD1 lentiviral interference vectors (shMLL2 and shLSD1 lentiviral vector) for follow-up experiments, and the construction method refers to the paper "Research on the Mechanism of Histone H3K4me2 on the Formation of Chicken SSCs" (He Nana. Research on the Mechanism of Histone H3K4me2 on the Formation of Chicken SSCs [D]. 2018. ) method, and the lentiviral packaging was completed by Shanghai Jiman Company.
[0167] ...
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