60 results about "Demethylase" patented technology

The invention discloses a radish bolting and flowering gene RsSUF4 regulated by m6A demethylase and application of the radish bolting and flowering gene RsSUF4, relates to the technical field of bioengineering, and is technically characterized in that the gene is RsSUF4. The functions of the demethylase RsALKBH10B and the flowering inhibition gene RsSUF4 in the flowering process are analyzed in a targeted manner, and a specific association mechanism between m6A modification and radish flowering regulation and control is defined.

The present disclosure relates to engineered RNA m6A demethylases with disrupted low complexity regions (LCRs) for expression in plants, as well as recombinant DNA encoding the engineered RNA m6A demethylases, vectors encoding the recombinant DNA, plants including the engineered RNA m6A demethylases, and method of improving the growth of plants using the engineered RNA m6A demethylases, including increased root growth and elevated photosynthesis. The present disclosure further relates to methods of improving growth of a plant, including increased root growth and elevated photosynthesis, by modifying endogenous ALKBH5 homolog genes to include disrupted LCRs, and plants produced by those methods.

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

The invention relates to a method for controlling wheat septospora which is resistant to a C14-demethylase inhibitor fungicide. The present invention relates to a method for controlling wheat septospora on a cereal plant, which wheat septospora comprises at least one mutation in the CYP51 gene and is resistant to a C14-demethylase (in sterol biosynthesis) inhibitor fungicide (DMI fungicide), the method comprises applying to these plants, seeds or soil of the plants a fungicidally effective amount of (I) methyl 2-[2-chloro-4-(4-chlorophenoxy) phenyl]-2-hydroxy-3-(1, 2, 4-triazol-1-yl) propionate or (II) 2-[2-chloro-4-(4-chlorophenoxy) phenyl]-2-hydroxy-3-(1, 2, 4-triazol-1-yl) propionic acid.

This invention relates to the application of ALKBH5 as a target in the preparation of drugs for inhibiting the progression of TNBC, and to the drugs themselves. This application discovers mRNA m 6 The alpha-modifying demethylase ALKBH5 is specifically highly expressed in triple-negative breast cancer (TNC). Using spontaneous TNC models and xenograft tumor models, knockout or knockdown of ALKBH5 significantly inhibited breast hyperplasia and the development of TNC, and promoted tumor differentiation. Simultaneously, the specific inhibitor EN300-14040 of ALKBH5 significantly inhibited the growth of TNC organoids, promoted TNC cell differentiation, and enhanced the sensitivity of TNC organoids to the endocrine drug 4OH-T, exhibiting a synergistic inhibitory effect with 4OH-T. Therefore, ALKBH5 represents a potential novel therapeutic target for TNC, and ALKBH5 inhibitors are a potential new type of drug for the treatment of TNC.

The present disclosure is concerned with substituted cyclopropyl carbamate and cyclopropyl sulfonamide compounds, pharmaceutical compositions comprising the compounds, and methods of using the compounds in the treatment of various cancers such as, for example, cancers comprising cells that express at least one Sox 2 stem cell marker. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention.

The present invention relates to a transgenic plant having increased biomass, yield, and / or abiotic stress tolerance. In particular, the present invention relates to a transgenic plant or plant cell into which a nucleic acid molecule encoding an RNA demethylase FTO (fat mass associated with obesity) protein is introduced. The present disclosure also provides methods of producing plants having increased biomass, yield, and / or abiotic stress tolerance.

This invention belongs to the field of gene editing and epigenetics technology, and discloses a method based on dCasRx-FTO for m 6 A. Editing system and its recombinant expression plasmid, cell model and its application; this invention first constructs an m based on dCasRx-FTO. 6 The A-editing system, namely the dCasRx-FTO fusion protein, utilizes catalytically inactivated CasRx (dCasRx) as a highly specific RNA guidance scaffold to fuse with m 6 A demethylase FTO enables the targeting of a specific region m 6 Targeted removal of A-modification. Building upon this, the present invention further obtains monoclonal mouse embryonic stem cells (mESCs) stably expressing the dCasRx-FTO fusion protein. Combined with an sgRNA library targeting a specific set of transcripts, high-throughput screening can be performed on embryonic stem cells containing the 2C-like pluripotency reporter gene MERVL::tdTomato, thereby identifying key m-modification inhibitors suppressing the pluripotency state of mESCs at the transcriptome level. 6 A modification site, thus enabling its use to reveal m 6 The key role of A-modified targets in the regulation of stem cell pluripotency.

The present disclosure relates to mutant enzymes and bacterial strains constructed to produce paraxanthine and 7-methylxanthine from caffeine and methods for the manufacture and use thereof.

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an m6A demethylase is introduced, wherein said m6A demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an m6A demethylase into a regenarable plant cell, and regenerating a transgenic plant from the regenerable plant cell.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

To provide a production method of lysine-specific demethylase (LSD1) inhibitors that is easier to implement and has higher yields.SOLUTION: A method for producing a compound represented by formula (I) or a pharmaceutically acceptable salt thereof comprises the step of converting a compound C to a compound D in a solvent under the action of an oxidizing agent.SELECTED DRAWING: None

The invention relates to a photo-enzyme catalytic reaction for producing methane through oxygen demethylation of aryl methyl ether and application of the photo-enzyme catalytic reaction. According to the method, demethylase MTase I and cobalamine binding protein CP which are purified by a nickel column are utilized, the demethylase MTase I, the cobalamine binding protein CP and 5-denitrified riboflavin react with different aryl methyl ether substrates respectively in a buffer solution with the pH value of 6.5 under the conditions of purple light irradiation and anaerobic conditions, O-demethylation of aryl methyl ether is catalyzed, and the 5-denitrified riboflavin is obtained. Demethylation products of methane and phenols are generated; according to the method disclosed by the invention, demethylation methane production reaction is completed, and meanwhile, the reaction efficiency and the substrate spectrum of oxygen demethylation of aryl methyl ether are improved. In-vitro catalysis of the demethylation methane production reaction of flavone aryl ether and natural biomass lignin alkaline hydrolysate is realized for the first time, and compared with the current demethylation reaction, the method has the advantages of mild reaction conditions, environmental protection and the like, and meanwhile, the removed methyl is converted into methane, so that the method is suitable for industrial production. The addition of additional methyl acceptors and the generation of byproducts are effectively avoided, and the atom economy is improved.

The present invention belongs to the field of biomedical technology, and specifically relates to siRNA that targets and inhibits the expression of the demethylase ALKB-1 gene or ALKBH1 gene and its application. The present invention uses Caenorhabditis elegans as a model, designs and synthesizes highly specific targeted siRNA for ALKB-1, and delivers it into the nematode body to achieve specific knockdown of ALKB-1, significantly delaying the aging process of Caenorhabditis elegans, and also has an improvement effect on aging-related diseases including Parkinson's. Through mechanism analysis, it was found that the anti-aging effect of specific siRNA targeted intervention of ALKB-1 is achieved by regulating the methylation modification of tRNA to affect protein homeostasis. The present invention provides a new reference method for the development of anti-aging drugs and gene therapy strategies based on siRNA targeted knockdown technology to intervene in the function of ALKB-1.

Provided herein are engineered pyocyanin demethylases having replacements in in positions A53, I73, A87, T91, M99, A129 and K141 of pyocyanin demethylase PodA of SEQ ID NO: 1 or a derivative thereof and related phenazine degrading agents, compositions, methods and systems, as well as a combined administration of one or more pyocyanin demethylases and antibiotics and / or antibiotics resulting in a synergic inhibition of viability of phenazine producing bacteria, and related phenazine degrading agents, compositions, methods and systems.

A method of decreasing conversion of nicotine to nornicotine is provided herein. The methods includes administering at least one basic region / leucine zipper (bZIP) type transcription factor inhibitor to an organism in need thereof. Also provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a bZIP type transcription factor binding site on a promoter of a nicotine N-demethylase (NND). Further provided herein is a method of decreasing conversion of nicotine to nornicotine including mutating a plant genome to knockout at least one bZIP type transcription factor.

This invention discloses a compound seed treatment agent containing metalaxyl-M and tebuconazole, its preparation method, and its uses, belonging to the technical field of seed treatment agents. The suspension of this invention uses metalaxyl-M, tebuconazole, and sodium selenite as the main components, supplemented with emulsifying and dispersing agents, wetting and dispersing agents, antifreeze agents, suspending agents, film-forming agents, and warning colors, and is prepared by adding deionized water to a sufficient volume. Metalaxyl-M targets and inhibits ribosomal RNA polymerase I of oomycete pathogens, while tebuconazole targets and inhibits sterol 14α-demethylase. The two have complementary fungicidal spectra, synergistically controlling maize stalk rot and maize head smut. Sodium selenite, by inhibiting the oxidative stress defense system of pathogenic fungi involving glutathione and thioredoxin, promotes the effects of the two main components and simultaneously remediates selenium-deficient soils. This invention is suitable for the green and efficient control of maize stalk rot and head smut, and also has the function of ecological restoration of selenium-deficient soils.

A specific super-resolution fluorescent probe for lysine-specific demethylase 1 and its application relate to the field of molecular probes. The present invention designs and synthesizes a specific small molecule fluorescent probe LP1 for lysine-specific demethylase 1. The probe is designed based on the active cavity configuration of lysine-specific demethylase 1 and the molecular structure of its specific inhibitor, and has excellent light stability, pH stability, and anti-interference ability against intracellular ions and amino acids. The product released after the probe LP1 reacts with lysine-specific demethylase 1 has an excellent near-infrared fluorescence signal. The probe LP1 successfully and specifically detects the activity of lysine-specific demethylase 1 in different biological samples (pure protein, cell lysate, living cells) and detects the subcellular organelle localization of lysine-specific demethylase 1.

The invention discloses a GLP-1-based FTO adjustment experiment device, which comprises two pieces of I-shaped steel, the upper ends of the two pieces of I-shaped steel are fixedly provided with a first fixing frame, a second fixing frame and a third fixing frame at equal intervals, and the first fixing frame is provided with a filter box; the method for adjusting the FTO experimental device based on the GLP-1 comprises the following steps: selecting 6-8-week-old mice, feeding the mice with high-fat diet for 12 weeks, establishing an atherosclerosis model, obtaining required animal tissues after the mice are killed, and carrying out subsequent experiments. The specific mechanism that GLP-1 regulates demethylase FTO to change the m6A methylation level of a differential gene so as to delay the atherosclerosis progress is deeply explored, an epigenetic regulation mechanism in the occurrence and development process of cardiovascular diseases is revealed, and a new theoretical basis is provided for research of the cardiovascular diseases; the field and thought of cardiovascular disease research are expanded, a new theoretical basis is provided for prevention and treatment of cardiovascular diseases, and the morbidity and mortality of the cardiovascular diseases can be reduced.

An m 6 A demethylase gene, named AhALKBH15 gene, the RNA sequence of which is shown as SEQ ID NO: 1, and the amino acid sequence encoded by the AhALKBH15 gene is shown as SEQ ID NO: 2. An anti-Ralstonia solanacearum resistance gene AhCQ2G6Y, the RNA sequence of which is shown as SEQ ID NO: 3. The amino acid sequence of the AhCQ2G6Y gene is shown as SEQ ID NO: 4. The m 6 A demethylase AhALKBH15 can remove the m 6 A modification, resulting in m 6 A level is reduced, the resistance gene AhCQ2G6Y is up-regulated, and the up-regulation of AhCQ2G6Y expression promotes the resistance of peanuts to BW. The great potential of AhALKBH15 for peanut resistance breeding is found.

The present application relates to a kind of molecular marker for diagnosing cancer, kit and its application, belong to biotechnology field.The present application provides the m 6 A modification as a molecular marker for diagnosing cancer, the m 6 A modification site is A923, A930 and A955, 3 m 6 A modification site is regulated by demethylase FTO and ALKBH5, promotes HRAS protein expression and the proliferation and metastasis of cancer cell.The present application first identifies the epigenetic transcription modification of HRAS and clarifies relevant molecular mechanism, provides new single / combination targeting treatment theoretical basis for RAS this "difficult drug target".

The invention belongs to the technical field of gene engineering, and particularly relates to a cre-FTO gene for improving the carbon sequestration efficiency of chlamydomonas reinhardtii, a related biological material and application. The cre-FTO gene disclosed by the invention is an RNA (Ribonucleic Acid) demethylase gene, and the nucleotide sequence of the cre-FTO gene is shown as SEQ ID NO: 1. The recombinant expression vector pDb124-cre-FTO containing the cre-FTO gene is transformed into the chlamydomonas reinhardtii, so that the photosynthetic efficiency, the carbon sequestration efficiency and the biomass of wild type chlamydomonas reinhardtii can be improved. The cre-FTO gene and related biological materials provided by the invention are helpful for improving the photosynthetic efficiency, starch conversion rate, pyruvic acid conversion rate and other carbon sequestration efficiencies of chlamydomonas reinhardtii, and have a more promising application value in improving the photosynthetic efficiency and the carbon sequestration efficiency.

Pesticidal mixtures comprising as active compounds 1) 1-[(1RS)-1,2-dimethylpropyl]-N-ethyl-5-methyl-N-pyridazin-4-yl-1H-pyrazole-4-carboxamide and 2) at least one further compound B selected from B.1) Succinate dehydrogenase inhibitors as defined in the specification; B.2) Inhibitors of oxysterol binding protein as defined in the specification; B.3) Inhibitors of complex Ill at Qi site selected from: a) florylpicoxamid, and b) fenpicoxamid; B.4) Strobilurins as defined in the specification; B.5) Inhibitors of C14-demethylase in sterol biosynthesis as defined in the specification; B.6) Inhibitors of cell division and cytoskeleton as defined in the specification, B.7) Other compounds of unknown mode of action as defined in the specification; and B.8) N-[[2-fluoro-4-[(2S,3S)-2-hydroxy-3-(3,4,5-trichlorophenyl)-3-(trifluoromethyl)pyrrolidin-1-yl]phenyl]methyl]cyclopropanecarboxamide; wherein component 1) and component 2) are present in a weight ratio of from 1000:1 to 1:1000; methods and use of these mixtures for combating invertebrate pests such as insects, arachnids, nematodes and / or harmful fungi in and on plants, and for protecting such plants being infested with pests and / or harmful fungi.

The application discloses a m6A demethylase-regulated radish bolting and flowering gene RsSUF4 and application thereof, relates to the field of bioengineering technology, and has the technical points that the gene is RsSUF4. The functions of the demethylase RsALKBH10B and the inhibited flowering gene RsSUF4 gene in the flowering process are analyzed, and the specific correlation mechanism between m6A modification and radish flowering regulation is determined.

The invention discloses optimization of a Cas12i3-5M-based soybean efficient multi-gene editing system and application of the Cas12i3-5M-based soybean efficient multi-gene editing system in creation of high-oleic-acid beany-smell-free germplasm resources. The system comprises a basic gene editing vector pSoC12i-V0, a promoter in a nuclease expression module is replaced by a soybean endogenous strong expression promoter GmM4pro, a human demethylase gene hsFTO synergistic module driven by GmM8pro is introduced, a tRNA processing ribozyme RNase P is adopted in a crRNA expression module for optimization, and the gene editing vector pSoC12i-V3 is constructed. The system can be used for efficiently and synchronously editing a plurality of target genes. A soybean variety 'Dongshen-89' is taken as a receptor, crRNA is designed for five genes of GmLOX1, GmLOX2, GmLOX3, GmFAD2-1A and GmFAD2-1B, and a new germplasm with high oleic acid content and no beany flavor, which is homozygically mutated at five targets, is successfully created. The invention provides an efficient gene editing tool and a new germplasm resource for soybean directional breeding and character polymerization.

The invention discloses a method for efficiently screening a functional enzyme and application of the functional enzyme, a demethylase is rapidly screened through matrix-assisted laser desorption / ionization mass spectrometry (MALDI-MSI) combined with non-targeted metabonomics and transcriptomics analysis, and the demethylase can be converted into dihydroberberine by taking palmatine as a substrate, so that the functional enzyme can be efficiently screened. And the enzyme mining efficiency and accuracy are obviously improved. The functional enzyme mining strategy provided by the invention solves the problem that more comprehensive information of natural metabolites and functional enzymes cannot be obtained by a traditional method, and is more beneficial to environmental protection and sustainable development.

The invention discloses a detection kit for demethylase FTO by using an unmarked biosensor based on a DNA fluorescent aptamer, which is characterized in that methylation modification is carried out on a specific base site on the DNA aptamer, so that the binding activity of the DNA aptamer is obviously inhibited, the DNA aptamer cannot be specifically bound with a fluorescent small molecule (DFHBI-1T), and only a weak fluorescent signal can be generated. Only when a target object FTO exists, the FTO can specifically remove methylation modification on the DNA aptamer, and the binding activity of Bibb Lettuce is recovered, so that the Bibb Lettuce is bound with DFHBI-1T with high specificity, and a significantly enhanced fluorescence signal is generated. The detection time of the kit disclosed by the invention is only 2 hours, and 43.2 nM of FTO can be detected; simple, rapid and economical detection of the demethylase FTO is realized, so that the problem that the clinical application of the existing detection method is limited due to the defects of complex probe synthesis process, high cost, insufficient light stability, serious autofluorescence interference, poor stability and the like is solved.

The present application provides a method for regulating the activity of functional nucleic acid, which comprises modifying one or more bases of the functional nucleic acid to inactivate the functional nucleic acid. Preferably, the functional nucleic acid or the substrate of the functional nucleic acid is modified to inactivate the functional nucleic acid, and then the modified group is removed to activate the functional nucleic acid. The present application also provides a deoxyribozyme molecular probe, which comprises domain a, domain b and domain c. The deoxyribozyme molecular probe is obtained by screening 6 A closed Dz, which realizes m 6 A high-efficiency and sensitive detection of demethylase and screening and detection of inhibitor small molecule drugs.

The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and / or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and / or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and / or Suv39h2.

The invention relates to a transgenic plant cell and a method for producing a transgenic plant. Specifically, the present invention relates to a transgenic plant cell which incorporates a nucleic acid molecule encoding an m6A demethylase (FTO protein) having two domains: an N-terminal domain (NTD) and a C-terminal domain (CTD) of AlkB oxidation demethylase function. The invention also relates to a method of producing a transgenic plant comprising introducing a gene encoding said m6A demethylase into a renewable plant cell, and regenerating the transgenic plant from said renewable plant cell.