22 results about "Demethylase" patented technology

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

The invention relates to a method for controlling wheat septospora which is resistant to a C14-demethylase inhibitor fungicide. The present invention relates to a method for controlling wheat septospora on a cereal plant, which wheat septospora comprises at least one mutation in the CYP51 gene and is resistant to a C14-demethylase (in sterol biosynthesis) inhibitor fungicide (DMI fungicide), the method comprises applying to these plants, seeds or soil of the plants a fungicidally effective amount of (I) methyl 2-[2-chloro-4-(4-chlorophenoxy) phenyl]-2-hydroxy-3-(1, 2, 4-triazol-1-yl) propionate or (II) 2-[2-chloro-4-(4-chlorophenoxy) phenyl]-2-hydroxy-3-(1, 2, 4-triazol-1-yl) propionic acid.

This invention relates to the application of ALKBH5 as a target in the preparation of drugs for inhibiting the progression of TNBC, and to the drugs themselves. This application discovers mRNA m 6 The alpha-modifying demethylase ALKBH5 is specifically highly expressed in triple-negative breast cancer (TNC). Using spontaneous TNC models and xenograft tumor models, knockout or knockdown of ALKBH5 significantly inhibited breast hyperplasia and the development of TNC, and promoted tumor differentiation. Simultaneously, the specific inhibitor EN300-14040 of ALKBH5 significantly inhibited the growth of TNC organoids, promoted TNC cell differentiation, and enhanced the sensitivity of TNC organoids to the endocrine drug 4OH-T, exhibiting a synergistic inhibitory effect with 4OH-T. Therefore, ALKBH5 represents a potential novel therapeutic target for TNC, and ALKBH5 inhibitors are a potential new type of drug for the treatment of TNC.

The present invention relates to a transgenic plant having increased biomass, yield, and / or abiotic stress tolerance. In particular, the present invention relates to a transgenic plant or plant cell into which a nucleic acid molecule encoding an RNA demethylase FTO (fat mass associated with obesity) protein is introduced. The present disclosure also provides methods of producing plants having increased biomass, yield, and / or abiotic stress tolerance.

The present invention is directed to a transgenic plant and a method for producing the same. In particular, the present invention is directed to a transgenic plant or a plant cell in which a nucleic acid molecule encoding an m6A demethylase is introduced, wherein said m6A demethylase has the following two domains: i) N-terminal domain (NTD) having the function of AlkB oxidation demethylase; and ii) C-terminal domain (CTD). The present invention is also directed to a method for producing said plant, comprising introducing a nucleic acid molecule encoding an m6A demethylase into a regenarable plant cell, and regenerating a transgenic plant from the regenerable plant cell.

This invention discloses a compound seed treatment agent containing metalaxyl-M and tebuconazole, its preparation method, and its uses, belonging to the technical field of seed treatment agents. The suspension of this invention uses metalaxyl-M, tebuconazole, and sodium selenite as the main components, supplemented with emulsifying and dispersing agents, wetting and dispersing agents, antifreeze agents, suspending agents, film-forming agents, and warning colors, and is prepared by adding deionized water to a sufficient volume. Metalaxyl-M targets and inhibits ribosomal RNA polymerase I of oomycete pathogens, while tebuconazole targets and inhibits sterol 14α-demethylase. The two have complementary fungicidal spectra, synergistically controlling maize stalk rot and maize head smut. Sodium selenite, by inhibiting the oxidative stress defense system of pathogenic fungi involving glutathione and thioredoxin, promotes the effects of the two main components and simultaneously remediates selenium-deficient soils. This invention is suitable for the green and efficient control of maize stalk rot and head smut, and also has the function of ecological restoration of selenium-deficient soils.

The present application relates to a kind of molecular marker for diagnosing cancer, kit and its application, belong to biotechnology field.The present application provides the m 6 A modification as a molecular marker for diagnosing cancer, the m 6 A modification site is A923, A930 and A955, 3 m 6 A modification site is regulated by demethylase FTO and ALKBH5, promotes HRAS protein expression and the proliferation and metastasis of cancer cell.The present application first identifies the epigenetic transcription modification of HRAS and clarifies relevant molecular mechanism, provides new single / combination targeting treatment theoretical basis for RAS this "difficult drug target".

The invention discloses optimization of a Cas12i3-5M-based soybean efficient multi-gene editing system and application of the Cas12i3-5M-based soybean efficient multi-gene editing system in creation of high-oleic-acid beany-smell-free germplasm resources. The system comprises a basic gene editing vector pSoC12i-V0, a promoter in a nuclease expression module is replaced by a soybean endogenous strong expression promoter GmM4pro, a human demethylase gene hsFTO synergistic module driven by GmM8pro is introduced, a tRNA processing ribozyme RNase P is adopted in a crRNA expression module for optimization, and the gene editing vector pSoC12i-V3 is constructed. The system can be used for efficiently and synchronously editing a plurality of target genes. A soybean variety 'Dongshen-89' is taken as a receptor, crRNA is designed for five genes of GmLOX1, GmLOX2, GmLOX3, GmFAD2-1A and GmFAD2-1B, and a new germplasm with high oleic acid content and no beany flavor, which is homozygically mutated at five targets, is successfully created. The invention provides an efficient gene editing tool and a new germplasm resource for soybean directional breeding and character polymerization.

The invention discloses a method for efficiently screening a functional enzyme and application of the functional enzyme, a demethylase is rapidly screened through matrix-assisted laser desorption / ionization mass spectrometry (MALDI-MSI) combined with non-targeted metabonomics and transcriptomics analysis, and the demethylase can be converted into dihydroberberine by taking palmatine as a substrate, so that the functional enzyme can be efficiently screened. And the enzyme mining efficiency and accuracy are obviously improved. The functional enzyme mining strategy provided by the invention solves the problem that more comprehensive information of natural metabolites and functional enzymes cannot be obtained by a traditional method, and is more beneficial to environmental protection and sustainable development.

The present application provides a method for regulating the activity of functional nucleic acid, which comprises modifying one or more bases of the functional nucleic acid to inactivate the functional nucleic acid. Preferably, the functional nucleic acid or the substrate of the functional nucleic acid is modified to inactivate the functional nucleic acid, and then the modified group is removed to activate the functional nucleic acid. The present application also provides a deoxyribozyme molecular probe, which comprises domain a, domain b and domain c. The deoxyribozyme molecular probe is obtained by screening 6 A closed Dz, which realizes m 6 A high-efficiency and sensitive detection of demethylase and screening and detection of inhibitor small molecule drugs.

The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and / or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and / or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and / or Suv39h2.

The invention relates to a transgenic plant cell and a method for producing a transgenic plant. Specifically, the present invention relates to a transgenic plant cell which incorporates a nucleic acid molecule encoding an m6A demethylase (FTO protein) having two domains: an N-terminal domain (NTD) and a C-terminal domain (CTD) of AlkB oxidation demethylase function. The invention also relates to a method of producing a transgenic plant comprising introducing a gene encoding said m6A demethylase into a renewable plant cell, and regenerating the transgenic plant from said renewable plant cell.

The invention relates to the technical field of stem cell engineering and anti-aging biology, in particular to a histone modification regulation method based on placenta amnion sub-totipotent stem cells and an anti-aging preparation thereof. The regulation and control method comprises the following steps: separating and purifying healthy full-month caesarean section placenta amniotic membrane tissues, carrying out composite enzymolysis and flow cytometry screening to obtain CD105 < + >, CD73 < + > and CD45 <-> stem cells, and primarily culturing the CD105 < + >, CD73 < + > and CD45 <-> stem cells; the method comprises the following steps: determining histone methyltransferase MLL3 and demethylase JMJD3 as target spots through ChIP-seq, and determining H3K4me3 and H3K27me3 as target modification sites; an MLL3 activator CM-272 with the final concentration of 15 [mu] M and a JMJD3 inhibitor GSK-J4 with the final concentration of 10 [mu] M form a composite regulator, and induction culture is performed for 84 h under the conditions of 37 DEG C and 5% CO2; and carrying out Western Blot verification and screening on the stem cells meeting the standard. According to the anti-aging preparation, regulated stem cells are used as active ingredients, a sodium alginate-gelatin (2: 1) composite carrier, 5% mannitol and 1% human serum albumin protective agent are used as auxiliary materials, and the injection with the pH of 7.2-7.4 is prepared. The method is accurate in regulation and control, and the preparation is remarkable in anti-aging effect and low in immunological rejection risk.

Therapeutic agents and treatment methods for urologic cancers, particularly urologic cancers with reduced lysine (K)-specific demethylase 6A (KDM6A) function, the agents and methods being characterized by suppressing both IL-6 activity and CCR2 / CCL2 activity, are provided.

The application discloses a kind of m6A demethylase ALKBH1 small molecule inhibitor, synthesis method and application, it is related to pharmaceutical technical field, the m6A demethylase ALKBH1 small molecule inhibitor has the chemical structural formula described in formula (I).The m6A demethylase ALKBH1 small molecule inhibitor of the application has biological activity, can be dose-dependent with ALKBH1 protein in vitro Binding, reduces ALKBH1 enzyme activity, has nanomolar level inhibitory activity, while the effect of the small molecule inhibitor to lung cancer cell A549 is verified, for subsequent screening by computer simulation, precise synthesis m6A demethylase ALKBH1 small molecule inhibitor and application in the disease caused by ALKBH1 overexpression provides broad application prospect.

The present invention relates to a therapeutic agent for use in treating diseases associated with gain of function of the androgen receptor (AR) and / or overexpression of an AR-activating cofactor, such as Kennedy's disease or cancer. The therapeutic agent of the present invention comprises at least one inhibitor of an androgen receptor (AR)-activating cofactor, selected from a protein arginine methyltransferase 6 (PRMT6) inhibitor, a lysine-specific demethylase 1 (LSD1) inhibitor, or a combination thereof.

The application discloses a molecular structure, a long G-quadruplex DNAzyme chain and a supramolecular NMM-G-quadruplex structure. The application further discloses a kit containing DNA1, DNA2 and a circular template. The application further discloses application of the molecular structure, the long G-quadruplex DNAzyme chain, the supramolecular NMM-G-quadruplex structure or the kit in detection of FTO and / or activity of a subfamily thereof. The application further discloses a method for detecting content / activity of FTO and / or a subfamily thereof. The application can accurately recognize and cut demethylated dsDNA, effectively eliminate background signals and improve detection specificity; can realize label-free and sensitive detection of FTO, and the detection limit is as low as 3.10*10 ‑16 M; and has wide application prospects in RNA demethylase related biomedical research and clinical diagnosis.

Provided herein are engineered pyocyanin demethylases having replacements in in positions A53, 173, A87, T91, M99, A129 and K141 of pyocyanin demethylase PodA of SEQ ID NO: 1 or a derivative thereof and related phenazine degrading agents, compositions, methods and systems, as well as a combined administration of one or more pyocyanin demethylases and antibiotics and / or antibiotics resulting in a synergic inhibition of viability of phenazine producing bacteria, and related phenazine degrading agents, compositions, methods and systems.

The application belongs to the field of molecular breeding, and specifically relates to m 6 A RNA methylation modification gene in creating new drought-resistant walnut germplasm, through the function research of a RNA methyltransferase gene and a demethylase gene, the core regulation role of m Juglans regia A in the drought resistance of walnut is revealed. 6 A methyltransferase gene JrMTA And demethylase gene JrALKBH9B The function research reveals that m 6 A modification plays a core regulation role in the drought resistance of walnut. JrALKBH9B The knockout by using CRISPR / Cas9 technology can significantly improve the m 6 A level of walnut, enhance the ability to remove reactive oxygen species (ROS) and reduce water loss, thereby greatly improving the drought resistance. JrMTA On the contrary, the loss of m 6 A leads to the decrease of the m A level, so that the plant is sensitive to drought. The application provides a key target for the molecular precise improvement of drought-resistant walnut varieties.

Provided herein are methods to treat fibrosis and / or inhibit angiogenesis.

Provided are a compound shown in general formula (I) or a tautomer, mesomer, racemate, enantiomer, diastereomer or mixture thereof, deuterium isotope derivative, pharmaceutically acceptable hydrate, solvate, salt or co-crystal thereof, a pharmaceutical composition thereof, and an application thereof in the preparation of a drug for a lysine-specific demethylase 1 inhibitor related disease.

The application discloses a label-free biosensor based on DNA fluorescent aptamer for a detection kit of demethylase FTO, methyl modification is carried out on a specific base site of a DNA aptamer (Bibb Lettuce), so that the binding activity of the DNA aptamer is obviously inhibited, the DNA aptamer cannot be specifically combined with a fluorescent small molecule (DFHBI-1T), and only a weak fluorescent signal can be generated. Only when the target object FTO exists, can the FTO specifically remove the methyl modification on the DNA aptamer, restore the binding activity of the Bibb Lettuce, make the Bibb Lettuce be highly specifically combined with the DFHBI-1T, and a significantly enhanced fluorescent signal can be generated. The detection time of the kit is only 2 h, and the kit can detect FTO to 43.2 nM; the kit is beneficial to realizing simple, rapid and economical detection of the demethylase FTO, and thus solves the problem that the clinical application of existing detection methods is limited due to the defects of a complex probe synthesis process, high cost, insufficient light stability, serious self-fluorescence interference and poor stability.