23 results about "Metabolic conversion" patented technology

The invention relates to a nerve repair guide catheter, a preparation method and application, an electrostatic spinning technology is adopted to construct a nanofiber membrane which is directionally and orderly arranged so as to guide nerve axons to directionally grow, and cell energy metabolism is promoted and tissue repair and regeneration are accelerated through metabolism and conversion of degradation products. Therefore, the nerve injury repairing effect better than that of traditional matrix materials without biological activity, such as PCL, is achieved. According to the invention, a novel solution is provided for solving the problem of insufficient biological activity of a traditional nerve conduit material by constructing a degradable high polymer material with biological activity. Therefore, the regeneration efficiency of peripheral nerve injury repair is improved, and the application prospect of the bioactive material in the fields of tissue engineering and regenerative medicine is expanded.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

A scalable and modular bioreactor defines a compact gas-liquid interface area populated with gas consuming microbes in a stacked arrangement for facilitating growth of the microbes in the presence of the greenhouse gases the microbes consume. A biofilm-based interface between liquid and gaseous regions of the reactor positions the microbes at the gas-liquid interface for metabolic exchanges of the gas for conversion into liquid and gaseous output. Microbes are selected for an affinity for a particular gas, such as methane (CH4), and metabolize carbon based outputs such as carbon biopolymers and gases such as carbon dioxide (CO2) and hydrogen (H2) for harvesting or recircling back for further metabolic conversions.

The invention discloses a high-flux toxicity pre-evaluation substitution method based on an acetylcholin esterase target. By integrating an SH-SY5Y nerve cell endogenous AChE and recombinant exogenous AChE dual detection system and relying on an automatic screening system constructed by integrating core equipment such as an automatic pipetting workstation, a constant-temperature incubator and a multifunctional microplate reader, a high-flux toxicity pre-evaluation substitution method based on an AChE target is successfully established and is used for evaluating the neurotoxicity of a compound, and the method has the advantages of high sensitivity, high sensitivity, high sensitivity and the like. The method solves the limitation that a single screening method in the prior art cannot evaluate factors such as target inhibition, cell permeability and metabolic transformation at the same time, and the method is simple, convenient, rapid, high in accuracy and suitable for large-scale primary screening of the AChE inhibitor and early warning of neurotoxicity risks.

The invention discloses a double-strategy screening method and system for active ingredients and target spots of medicinal and edible food, and belongs to the field of food science and computational chemistry. Aiming at the problems that a traditional molecular docking process is complex, the screening efficiency is low, metabolic transformation is ignored, intelligent scoring and research and development link splitting are lacked and the like, a multi-source database is integrated to construct a chemical component matrix, and double-strategy screening is adopted: target screening is combined with a metabolic enzyme system rule system and heterogeneous graph neural network scoring to recognize an active target; component screening is based on structural priori of a graph neural network, a ligand conformation library is generated by means of a conditional diffusion model, and activity and toxicity are predicted in combination with a multi-task graph neural network Food-GNN. Finally, the formula is optimized through an inference engine and an NSGA-III multi-target algorithm, end-to-end automation is achieved, the screening efficiency and target spot prediction accuracy are improved, and support is provided for modern development of medicine and food homology food.

The invention relates to the technical field of single cell analysis of cell biology and analytical chemistry, in particular to a flow type single cell scale adjustable device for electrospray ionization mass spectrometry and an adjusting method. According to the present invention, the detection requirements of multi-size cells can be met, the target cells with different sizes in the range of 10-100 [mu] m can be screened through the arched channel switching of the arched shunting capture module, and the comprehensiveness of the detection sample can be ensured. According to the device, the arched channels can be rapidly switched to adapt to cells of different sizes without disassembling the device, continuous operation of sample introduction, screening, extraction, ionization and detection is realized through integrated flow design, high throughput and convenience are taken into account, and the device is adaptive to diversified environmental pollutant exposure metabolism research scenes. The cell suspension and the diluent are mixed on line to reduce non-volatile salt interference, the high-voltage electric field and the sheath gas cooperate to improve ionization efficiency, qualitative and quantitative analysis is achieved through orbitrap mass spectrometry, reliable data support is provided for accumulation, distribution and metabolic transformation characteristics of pollutants in cells, and detection accuracy is guaranteed.

The invention relates to the technical field of metabolite detection, and particularly discloses a method for detecting trimethylamine in an excrement sample. By improving a sample treatment method and optimizing headspace-gas chromatography conditions, the invention provides the method for measuring trimethylamine in the excrement sample, which is simple and convenient to operate, high in accuracy, excellent in precision and strong in applicability. According to the detection method, an acidic medium-chain alcohol extract liquid compounded by n-hexanol and an inorganic strong acid dilute solution is selected to treat an excrement sample. The acidic medium-chain alcohol extract liquid not only can efficiently extract, enrich and fix trimethylamine in a sample, but also can block metabolic transformation of trimethylamine and a precursor thereof by quenching a microbial reaction, so that the anti-interference capability is improved. In cooperation with optimized headspace-gas chromatography detection conditions, the detection sensitivity and accuracy are further improved, and a stable and reliable technical support is provided for risk assessment of diseases related to intestinal flora metabolism.

The invention discloses a 2, 3-difluoro-4-(trans-4-propylcyclohexyl) butoxybenzene exposure biomarker and application of the 2, 3-difluoro-4-(trans-4-propylcyclohexyl) butoxybenzene exposure biomarker in human body load monitoring, and the exposure biomarker is more than one of compounds shown in a table 4. According to the invention, based on the I / II phase metabolic transformation rule of BDPRB, the full-spectrum screening of the exposed biomarker is realized by combining high-resolution mass spectrum data and a fragment ion search system; meanwhile, through metabolite peak area semi-quantitative analysis, the high-sensitivity exposure marker for monitoring the load in the human body is determined for the first time. According to the method, a potential target is locked through metabolic site energy simulation, and accurate molecular structure analysis of the BDPRB exposed biomarker can be realized by utilizing a fragment ion spectrum intelligent matching algorithm.

The invention relates to an in-vitro evaluation method and system for metabolism and colon toxicity of compound pollutants in water, which are used for efficiently evaluating the compound pollutants in water. Firstly, a three-stage anaerobic reaction system formed by connecting oral cavity, stomach and intestine digestion simulation tanks in series is used for continuously simulating metabolic transformation of pollutants in an empty-belly water drinking state; secondly, enriching and concentrating intestinal digestion products by adopting a solid-phase extraction-nitrogen blowing-redissolving three-step method, and eliminating the interference of digestive enzyme residues; and finally, co-exposing the concentrate and the pre-cultured colon-like organ, and evaluating the colon toxicity effect. According to the scheme, a drinking water targeted digestion simulation system is initiated, the limitation of a food digestion model is broken through, multiple technologies are coupled to form closed-loop evaluation, the single experimental period is less than or equal to 14 days, 99% or above of digestive enzyme residues can be efficiently eliminated, large sample parallel processing is supported, and the physiological correlation and experimental efficiency of an evaluation result are remarkably improved; and the method is suitable for high-flux colon toxicity evaluation of multiple composite pollutants in water.

The invention discloses a method for detecting a Wantongkang in-blood core bioactive component group and verification, serum, urine and excrement are collected as an experimental group after a preset time, and serum, urine and excrement of animals not given with Wantongkang are used as a control group; and detecting the experimental group and the control group by adopting liquid chromatography-mass spectrometry. The invention systematically analyzes the original components and metabolites of Wantongkang in blood plasma, urine and excrement of rats. 38 primordial components are detected in plasma, 48 urine and 89 excrement. The terpenoids may form a core pharmacodynamic material basis of WTYK for treating pharyngitis. A metabolic conversion path of picria fel-terrae glycoside IA, rosmarinic acid, isofraxidin, caffeic acid and apigenin-7-O-glucuronide is constructed through metabolic conversion analysis, and active metabolites of picria fel-terrae glycoside IA, rosmarinic acid, isofraxidin, caffeic acid and The network pharmacological research based on the in-blood component reveals that the WTYK plays a therapeutic role in treating the pharyngitis mainly by regulating key signal channels such as PI3K-Akt, MAPK, TNF, JAK-STAT and NF-kB, wherein the NF-kB signal channel is closely related to the occurrence and development of the pharyngitis.

The invention relates to the technical field of food nutrition assessment and biotechnology crossing, in particular to a food nutrient biological value assessment method based on an intestinal organ model. The method comprises the following steps: S1, pre-treating food to be detected by adopting an in-vitro simulated gastrointestinal digestion system to generate a digestion product; and S2, introducing the digestion product into an intestinal organ-like culture system, simulating intestinal peristalsis and fluid movement between villi through a precise perfusion device, and carrying out dynamic co-culture. By combining the in-vitro simulated gastrointestinal digestive system and the dynamic co-culture system of the intestinal organs, the digestion and absorption process of the human intestinal tract can be simulated more truly; by monitoring kinetic parameters such as nutrient uptake, metabolic transformation, bioavailability and the like, high-flux and multi-dimensional evaluation on the biological value of the food nutrient can be realized, and the limitations of a traditional animal model or a static cell model in the aspects of prediction accuracy, flux and ethics are overcome.

The invention relates to the technical field of health management, in particular to a blood glucose and weight collaborative management method based on dynamic periodic glucose interruption intervention, which comprises the following steps: acquiring blood glucose data and weight data of a user; based on a comparison result of the blood glucose data and a personalized threshold value, dynamically triggering a high-strength sugar breaking period or a maintenance low-sugar period; performing extremely low-carbon water diet intervention in the sugar break period, and performing moderate low-carbon water diet intervention in the low-sugar period; and adjusting intervention parameters of the next period according to the weight change trend. According to the blood glucose and body weight collaborative management system and method based on dynamic periodic glucose interruption intervention, a glucose interruption-low glucose periodic alternating mechanism is created, metabolic conversion is induced by using a glucose interruption period, metabolic flexibility is consolidated and improved by using a low glucose period, and individual differences are adapted based on dynamic stage switching of a personalized blood glucose threshold value; through hardware cooperation, blood sugar and weight double-index real-time correlation analysis is realized, and personalized scheme making, special dietary food intelligent recommendation, real-time data monitoring and dynamic adjustment are realized.

A scalable and modular bioreactor defines a compact gas-liquid interface area populated with gas consuming microbes in a stacked arrangement for facilitating growth of the microbes in the presence of the greenhouse gases the microbes consume. A biofilm-based interface between liquid and gaseous regions of the reactor positions the microbes at the gas-liquid interface for metabolic exchanges of the gas for conversion into liquid and gaseous output. Microbes are selected for an affinity for a particular gas, such as methane (CH4), and metabolize carbon based outputs such as carbon biopolymers and gases such as carbon dioxide (CO2) and hydrogen (H2) for harvesting or recircling back for further metabolic conversions.

The invention belongs to the technical field of detection pretreatment equipment in multiple fields of food, non-food and the like, and provides an efficient and accurate multi-field sample preparation and separation device and a use method thereof.The efficient and accurate multi-field sample preparation and separation device comprises a treatment cylinder, a top cover is arranged at the top end of the treatment cylinder, and a smashing and homogenizing mechanism is arranged above the top cover; a discharging assembly is arranged below the processing barrel, supporting seats are fixedly connected to the bottom face of the processing barrel, and a weighing assembly is fixedly installed on the side faces, close to each other, of the two supporting seats. The variable frequency motor drives the driving rod, the driving disc and the smashing cutter to efficiently rotate, a homogenizing structure can be formed, one-stop rapid sample homogenizing can be achieved in cooperation with auxiliary liquid supply of the water injection electromagnetic valve and the water injection pipe, subsequent cryopreservation and repeated freezing and thawing are not needed, it is avoided that the biological activity of a sample induces drug metabolic conversion or structural isomerization, and the efficiency is high. And the detection result reflects the original residual state.

The invention provides a fermented soybean meal laying hen feed and a preparation method thereof, and belongs to the technical field of feeds. According to the application, nutrition absorption, intestinal health and metabolism regulation are taken as a core, the introduced fermented soybean meal degrades anti-nutritional factors through synergistic fermentation, small peptides and free amino acids which are easy to absorb are provided, the egg white protein synthesis efficiency is improved, and intestinal flora is optimized; the introduced composite organic acid-probiotic liquid additive is combined with organic acid and probiotics, so that the intestinal environment is improved, mineral absorption is enhanced, and pathogenic bacteria are inhibited; the added methionine / vitamin D3 mixed microcapsule accurately releases nutrition through a slow release and embedding technology, and synergistically promotes egg white protein synthesis and eggshell mineralization, and the three components are linked, so that nutrition absorption, intestinal health, metabolic transformation and reproductive output are optimized; meanwhile, by-products such as brown sugar and carrot residues are utilized, use of antibiotics is reduced, and the green feed development trend is met.

The invention relates to the technical field of metabolism simulation, in particular to a static simulation method for the metabolic process of pollutants coupling fish enteric microorganisms and digestive enzymes, which comprises the following steps: acquiring a fish enteric microorganism enrichment culture and an enteric enzyme extract, and analyzing the abundance of a fish enteric microorganism community and the variety of digestive enzymes; a static model composed of a gastric phase compartment, a midgut compartment and a hindgut compartment is constructed, and key incubation parameters such as culture medium volume, pH value, temperature, digestive enzyme activity and bile salt concentration of each compartment are determined. In the invention, the preparation and pre-stabilization method of the intestinal microbial liquid is particularly optimized, the authenticity of microbial metabolic activity is ensured, and the pairing relationship between pollution conversion and different digestion stages of fish is established by constructing a pollutant static metabolic conversion method of microbial community and digestive enzyme. The evaluation accuracy of the first-pass metabolic reaction of the pollutants is improved, and structural information of microorganisms, enzymes, the pollutants and substrates is integrated.

The invention discloses a flax polyphenol composition for regulating and controlling absorption and metabolic transformation of alpha-linolenic acid and application, and relates to the technical field of nutrition metabolism. Comprising the following components: any one or more of flax lignan macromolecules FLM, ring-opening isolariciresinol diglucoside SDG or ring-opening isolariciresinol SECO. The invention discloses a unique synergistic effect (for example, low-dose lignan optimizes interfacial properties, moderately inhibits digestive enzyme to improve absorption and differentially regulates expression of a liver invertase system) of polyphenols (FLM, SDG and SECO) of different structural units and a nano delivery system, and establishes a new mode of regulating ALA metabolic transformation based on multiple targets of intestine-liver-flora.

The invention discloses a method for segmented fermentation of soybean meal feed by using bacillus coagulans, and belongs to the technical field of feed processing. The preparation method comprises the following steps: preparing a bacillus coagulans seed solution, inoculating a soybean meal raw material with the bacillus coagulans seed solution, and carrying out first-stage and second-stage fermentation. The bacillus coagulans strain adopted by the invention still has good adaptability and fermentation capacity in a complex feed matrix environment, and through first-stage fermentation, it is ensured that an initial flora quickly occupies a micro-ecological site in the initial stage of fermentation, so that the growth of infectious microbes is inhibited, the primary degradation reaction of a substrate is accelerated, and the degradation rate of the substrate is increased; in the first stage fermentation, effective degradation of anti-nutritional factors and preliminary improvement of raw material structures are ensured, the second stage fermentation strengthens metabolic conversion of substrates and accumulation of beneficial products and promotes synthesis accumulation of beneficial metabolites such as lactic acid and short-chain fatty acid, and staged fermentation not only realizes deep degradation of the anti-nutritional factors, but also promotes accumulation of the beneficial metabolites, so that the anti-nutritional factors can be effectively degraded. The functionality and the nutritional value of the soybean meal feed are obviously improved.

The invention belongs to the technical field of microbial metabolic engineering modification. The invention provides an acetyl coenzyme A synthase mutant. The amino acid sequence of the acetyl coenzyme A synthase mutant is shown as SEQ ID No.1 in a sequence table. The acetyl coenzyme A synthase mutant provided by the invention is remarkably improved in the aspects of improving acetic acid metabolic activity and acetylation resistance, on one hand, the acetyl coenzyme A synthase mutant can be used for improving the efficiency that acetic acid is metabolized and converted by a strain, and on the other hand, accumulation of a byproduct acetic acid in a biological metabolism process can be reduced.

This invention belongs to the field of component detection, specifically a method and system for intelligent detection and analysis of components of traditional Chinese medicine entering the bloodstream. The method includes: acquiring the chromatographic-mass spectrometry data stream of a traditional Chinese medicine sample entering the bloodstream; identifying preliminary sub-peaks and determining their initial parameters; constructing an asymmetric peak shape model to characterize the sub-peak profiles, iteratively optimizing the parameters using first and second optimization algorithms to minimize the sum of squared residuals between the reconstructed peak shape and the original data, and selecting characteristic peaks with acceptable signal-to-noise ratios; constructing an adaptive mother wavelet based on the refined parameters of the characteristic peaks, and performing a second continuous wavelet transform on the preprocessed data; merging all sub-peaks and performing global joint optimization using the model parameters as variables, matching metabolic transformation rules based on the difference in the exact mass number of peak pairs, evaluating the rationality of chromatographic behavior and abundance ratios, and applying rewards and penalties; and calculating the peak area, peak height, and exact retention time of each sub-peak based on the parameters after global optimization convergence.

The invention belongs to the technical field of food biology, and particularly relates to an anti-aging product rich in collagen peptide and an enzyme-bacterium synergistic production method of the anti-aging product. According to the present invention, swimming bladder is adopted as a raw material, blueberry is adopted as a carrier, protein precise enzyme digestion, flavor quantitative recombination, function targeting regulation and other technologies are integrated, and the synergistic effect between pepsase and plant lactobacillus is utilized to create the new generation of fermentation type fish source collagen peptide beverage; the product is free of any food additive and high in food safety; the color is purplish red and bright, the texture is uniform and stable, the taste is moderately sour and sweet, the smell is strong and harmonious, and the sensory quality is high; and by utilizing the synergistic effect among the collagen peptide, the blueberry nutritional functional components, the probiotics and metabolic conversion products thereof and other components, the physiological efficacy is high. The technical product breaks through the pain points of low sensory quality, poor food safety, low physiological efficacy, lack of high-end products, serious homogenization involute and the like of the current collagen peptide product, and is beneficial to sustainable and high-quality development of the industry.

The present invention relates to a method for screening for an equol producer by using personalized pharmaceutical meta-analytical screening (PMAS) technology. According to the method of the present invention, soybean isoflavone bioconversion by an intestinal microorganism is analyzed such that whether the microorganism is an equol producer can be determined. Therefore, by screening the metabolic conversion ability of the main components of soybeans, the difference in effects of individual functional materials can be easily determined.

This invention discloses urinary biomarkers for p-hydroxyacetophenone (PHAC) exposure and their application in human load monitoring. Based on the phase I / II metabolic transformation patterns of PHAC, this invention establishes a targeted screening list, enhances mass spectrometry fragment acquisition efficiency and qualitative analysis capabilities, and combines high-resolution mass spectrometry data with a fragment ion search system to achieve full-spectrum screening of urinary exposure biomarkers. Furthermore, it identifies potential targets through metabolic site energy simulation and utilizes a fragment ion mapping intelligent matching algorithm to achieve precise molecular structure and metabolic transformation pathway analysis of six PHAC urinary exposure biomarkers. Simultaneously, through semi-quantitative analysis of metabolite peak areas, it establishes, for the first time using non-invasive urine samples, highly sensitive PHAC exposure biomarkers suitable for human load monitoring.