55 results about "Methyl selenocysteine" patented technology

This invention discloses a method for synthesizing seleno-methyl selenocysteine with alpha amido crylic acid derivant, in which, methyl-hydroselenide or its salt solution generates addition reaction with alpha-amido acrylic acid derivant to generate beta-methyl-alpha-amido methyl acetic acid derivant, then the ester compound in it is hydrolyzed and saponified by NaHCO3, NaOH or KOH and acidified by HCl or H2SO4 to get its carboxy compound, then the N-acyl in it is hydrolyzed and taken off by HCl or H2SO4 plus hot water to get beta-methyl-alpha-amido propionate or sulfate and finally neutralized with ammonia or TEA to get seleno-methyl selenocysteine.

Provided is a method for cultivating selenium-enriched wheat plant materials. The selenium content in selenium-enriched garlic plant materials is 1 mg/kg to 1000 mg/kg, and selenium which exists in an organic selenium (methyl selenocysteine) form accounts for 85% of the total selenium content. The method is characterized by comprising the steps that selenium-enriched nutrient solution with a selenium source is applied at the elongation stage of wheat, and meanwhile, the effective quantity of selenium source adsorbents or catalysts for physical adsorption of the selenium source are added. According to the method, stable and effective selenium source supply is provided for wheat to promote absorption and accumulation of selenium in wheat, the selenium element is effectively transformed to the organic selenium form from the inorganic selenium form, and the risk that excessive selenium poisons the wheat growth environment is avoided. The method solves the problems that in the prior art, selenium durability in plant materials is short, and selenium is prone to being reduced in the environment and is low in utilization rate.

The invention discloses a method utilizing enzyme measure to separate a mixed rotating selenium-methyl selenocysteine to prepare an optical pure selenium-methyl selenocysteine. The method acerylates the mixed rotating selenium-methyl selenocysteine with erhyl anhydride in sodium hydroxide solution and acidificates with hydrochloride to achieve a mix N ethyl acyl selenium methyl selenocysteine. Then, in certain PH value, reaction temperature, enzyme amount, substrate and cobalt ion concentration, the mix N ethyl acyl selenium methyl selenocysteine is separated by acylate enzyme I of swine renal to obtain an oprical pure L selenium methyl selenocysteine and an N ethyl D selenium methyl selenocysteine. And finally the N ethyl D selenium methyl selenocysteine is heated and hydrolyzed with hydrochloride and is neutralized with ammonia water to obtain an optical pure D selenium methyl selenocysteine. The method has the advantages of convenient for operating and low in cost, which is suitable for industrialized production of the optical pire selenium methyl slenocysteine.

The present invention belongs to the technical field of health-care products and more particularly relates to a selenium-enriched polypeptide health-care product and a preparation method thereof. The selenium-enriched polypeptide health-care product comprises: 0.1-0.2 part of L-selenium-methyl selenocysteine, 50-300 parts of polypeptides, 3-10 parts of vitamin E, and balance accessory materials consisting of 200-300 parts of wheat starch and 20-80 parts of maltodextrin. The preparation method of the selenium-enriched polypeptide health-care product comprises the following steps: step (1): 24.4 kg of starch, 0.5 kg of the vitamin E and 20 kg of the polypeptides are taken, the taken materials are stirred in a mixer for 15 min to be mixed evenly; step (2):10 g of the L-selenium-methyl selenocysteine and 5 kg of the maltodextrin are taken, 8 L of water is added into the taken materials and the taken materials are dissolved evenly; and step (3): a solution prepared in the step (2) and an obtained mixture in the step (1) are stirred for 10 min, and the materials are mixed and granulated to obtain the selenium-enriched polypeptide health-care product particles. The selenium-enriched polypeptide health-care product is easy to produce and low in costs, has very good regulation and improvement effects on immunity of body, is free of has toxic and side effects, and dependence, and safe and reliable, protects body health and improves quality of life.

The invention relates to an embedding and diluting method for L-selenium methyl selenocysteine. The method comprises the following steps: by taking high purity L-selenium methyl selenocysteine as a raw material, assisting beta-cyclodextrin to embed the L-selenium methyl selenocysteine by virtue of an ultrasonic method; then, by taking starch, dextrin, sodium carboxymethylcellulose and xanthan gum as auxiliary materials, boiling and granulating the mixture for secondary embedment; and finally forming a low purity L-selenium methyl selenocysteine microcapsule finished product which is stable in property and free of objectionable odors. The method provided by the invention has the advantages that the microcapsule is short in forming time and the efficiency is high; the production process is low in energy consumption and small in pollution; the product is good in embedding effect, stable in property and good in dispersibility after being dissolved; the low purity L-selenium methyl selenocysteine microcapsule finished product facilitates addition of selenium element into a health-care food, and the product added with the selenium element is good in mixing uniformity.

The invention discloses selenium fortified nutritional wheat meal containing L-selenium-methyl selenocysteine. L-selenium-methyl selenocysteine intensifying agents are prepared into L-selenium-methyl selenocysteine intensifying agent premix feeds, and each ton of wheat meal main ingredients are mixed with 1kg of L-selenium-methyl selenocysteine intensifying agent premix feeds. The L-selenium-methyl selenocysteine intensifying agent premix feeds comprise 0.5g of L-selenium-methyl selenocysteine, 99.5g of light tricalcium phosphate flow carriers, 200g of wheat starch diluents and 700g of modified starch diluents, and the sum of all components is 1000g. The purity of the L-selenium-methyl selenocysteine is 96%, the L-selenium-methyl selenocysteine contains 43% of selenium, and the content of the selenium in a finished product is 140-280 micrograms/kg. The selenium fortified nutritional wheat meal is simple in process, convenient to process and operate, stable in product quality, easy to popularize and suitable for industrial production, micronutrients are uniformly added, continuous proportioning production can be realized, and selenium micronutrients required by a human body can be effectively supplemented.

The invention relates to a method for preparing an amino acid drug, and in particular to a method for preparing optically pure L-type selenium-methyl selenocysteine. The method comprises the steps ofmixing N-acetyl-3-chloro-L-serine methyl ester with methylselenol salt to obtain N-acetyl-3-methyl selenyl-L-serine methyl ester; mixing with a hydrochloric acid solution and carrying out hydrolysis reaction to obtain selenium-methyl selenocysteine; dissolving the selenium-methyl selenocysteine in water, adding a solvent A and uniformly mixing; adding activated carbon to decolorize, filtering andcollecting filtrate; and adding a solvent B into filtrate, uniformly mixing, cooling, standing for more than 1 hour and collecting crystals to obtain the optically pure L-type selenium-methyl selenocysteine. The method disclosed by the invention has the characteristics of being simple in process, high in product optical purity, green and friendly to environment and is suitable for large-scale industrial production.

The invention discloses selenium-rich crispy bone flavored Braseniaschreberi J.F.Gme1. and a preparation method thereof. The selenium-rich crispy bone flavored Braseniaschreberi J.F.Gme1. consists of enzymatic hydrolysate of Braseniaschreberi J.F.Gme1. stems and leaves, bone soup, flour, Braseniaschreberi J.F.Gme1. colloid, water, sodium chloride, helianthus tuberosus, monosodium glutamate, L-selenium-methyl selenocysteine and selenium-rich yeast powder. The preparation method comprises the following steps: (1) picking fresh Braseniaschreberi J.F.Gme1., washing, and processing by a high-pressure kettle, thereby obtaining the Braseniaschreberi J.F.Gme1. colloid and the enzymatic hydrolysate of Braseniaschreberi J.F.Gme1. stems and leaves; (2) smashing livestock/poultry bones and removing floating oil; (3) adding the raw materials such as the enzymatic hydrolysate of Braseniaschreberi J.F.Gme1. stems and leaves, mixing uniformly, and adding L-selenium-methyl selenocysteine and the selenium-rich yeast powder to obtain a dough; (4) shaping the dough on a die; (5) freezing and concentrating the dough, and directly performing vacuum low-temperature frying without unfreezing; and (6) performing oil removal treatment to obtain the selenium-rich crispy bone flavored Braseniaschreberi J.F.Gme1.. The selenium-rich crispy bone flavored Braseniaschreberi J.F.Gme1. has rich nutrition and high content of organic selenium, protein and acid heteropolysaccharide active substances, and particularly, the selenium content is as high as 180-240 microgram/Kg; the selenium-rich crispy bone flavored Braseniaschreberi J.F.Gme1. has the advantages of crispy mouthfeel, bright color and complete nutrition and can be easily absorbed by a human body.

The invention discloses a method for preparing racemic selenium-methyl selenocysteine by using aldehyde catalysis. The method comprises the following steps: dissolving selenium-methyl selenocysteine in glacial acetic acid or acetic acid water solution, adding an aldehyde catalyst into the water solution, heating and stirring the mixture to react, monitoring optical rotation, stopping the reaction after the racemization is finished, reducing the pressure and evaporating a solvent to obtain a solid, washing the solid by methanol, performing vacuum filtration on the washing solution, and drying the washing solution in vacuum at the normal temperature to obtain the racemic selenium-methyl selenocysteine. The method has the advantages of convenient operation and low cost, and is suitable for the industrialized production of the racemic selenium-methyl selenocysteine.

The invention discloses a special shiitake mushroom nutrient microorganism base fertilizer which is characterized by being prepared from the following raw materials in parts by weight: 3.1-3.2 parts of pine-based biomass charcoal, 0.1-0.2 part of sodium tripolyphosphate, 1.5-1.6 parts of schisandra chinensis oil, 0.96-1 part of povidone, 2.1-2.2 parts of L-selenium methyl selenocysteine, 0.8-0.9 part of sodium silicate, 1.2-1.3 parts of monopotassium phosphate, 1.2-1.3 parts of polyglutamic acid and 0.7-0.8 part of dodecyl dimethyl betaine. The fertilizer is rich in nutrition, can be rapidly absorbed and utilized and is capable of improving crop activity and metabolism, increasing contents of beneficial functional substances and promoting growth.

The invention belongs to the technical field of agricultural microorganisms, and particularly discloses an LX-88 strain with good selenium conversion and Se (0) oxidation capacity and application of the LX-88 strain, and the preservation number of the strain is CCTCC NO: M2021542. The strain can convert SeMet into selenocystine (SeCys2), methyl selenocysteine (MSeCys) and the like and has an oxidation effect on elemental selenium, and the strain LX-88 can be used for reducing Se (IV) and can be used for reducing the Se (IV) into red nano-selenium (SeNPs), SeCys2 and MSeCys. Therefore, the strain LX-88 can be used as an industrial microorganism and is applied to production and preparation of SeCys2 and MSeCys; the strain can be used as a model microorganism for research on metabolic transformation of organic selenium and Se (IV); the plant selenium-rich microbial inoculum can be prepared and can be used as a microorganism for Se (0) oxidation research.

The invention discloses a method for analyzing the selenium form of selenium-rich proteoglycan based on HPLC-ICP-MS. Protease K and a Tris-HCl buffer solution are added into the selenium-enriched proteoglycan, enzymolysis is performed for 18 hours at the constant temperature of 37 DEG C, and high performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS) is applied to analyze selenium compounds in enzymolysis supernatant: sodium selenite (Na2SeO3), selenocystine (SeCys2), selenium methyl selenocysteine (SeMeCys) and selenomethionine (SeMet). According to the method, only four selenium compounds are obtained through enzymolysis and are highly matched with a standard substance, and quantification is accurate; and the recovery rate is 78.35-111.42%, and the method is high in recovery rate, mild in condition and good in stability.

The invention provides a method for biosynthesis of organic selenium by using Hypsizygus marmoreus. The invention provides a Hypsizygus marmoreus mutant strain which can be obtained through ultraviolet mutagenesis, inorganic selenium in a culture medium can be converted efficiently into organic selenium through the Hypsizygus marmoreus mutant strain, and the strain has a preservation number of CCTCC M 2019981, wherein Hypsizygus marmoreus has short fruiting time and short growth time, controllable culture conditions, controllable addition amount of selenium and a stable growth state. Sodium selenate, sodium selenite and nano selenium are preferably adopted as the addition forms, no effects on the biomass of fruiting bodies of Hypsizygus marmoreus are caused under the condition of a concentration of 40 mg/kg, but increase of the yield of the fruiting bodies of Hypsizygus marmoreus can be promoted to a certain extent, wherein selenium-methyl selenocysteine can reach up to 39.4% of the total content of selenium. During culture, mycelia of Hypsizygus marmoreus grow fast, fungus bags can be full in a short time, and Hypsizygus marmoreus has white and tender color and high yield, so thata powerful means is provided for preparation of selenium-methyl selenocysteine and other organic selenium.

The invention relates to the technical field of biological detection, in particular to a method for determining selenomethionine in a biological matrix. The method for determining selenomethionine ina biological matrix provided by the invention comprises steps of: (1) sample pretreatment: taking a biological matrix sample, adding internal standard methyl selenocysteine, then treating the biological matrix sample with a protein precipitation method, and centrifuging to obtain a supernatant; (2) content determination: injecting the supernatant in the step (1) into a chromatograph by using highperformance liquid chromatography tandem mass spectrometry (LC-MS/MS) chromatography to calculate the concentration of selenomethionine in the biological matrix sample. The extraction efficiency of selenomethionine is high, and the biological matrix does not interfere with the determination of selenomethionine by adopting the protein precipitation method in the biological matrix of the invention;and the speed is fast, the special property is good, the accuracy is high and the reproducibility is good by determining selenomethionine in the biological matrix with the LC-MS/MS chromatography.

The invention discloses a culture medium for promoting growth of scenedesmus and accumulation of lutein. The culture medium comprises a carbon source, a nitrogen source, phosphate, a calcium salt, aniron salt, a plurality of trace elements and vitamins according to a specific proportional ratio, and also comprises valine, leucine, L-selenium-methyl selenocysteine, silver thiosulfate, diphenylureasulfonic calcium, IAA (indoleacetic acid), alpha-ketoglutaric acid, clethodim, nicotine and spinach juice. The culture medium disclosed by the invention has the advantages that by optimizing the carbon source and the nitrogen source, and adding a growth regulator, a precursor compound synthesized and metabolized by the lutein, and a plurality of means for facilitating the accumulation of the lutein, the biomass of the scenedesmus is obviously increased, and the synthesizing and accumulation of the lutein are also obviously increased; by adopting the innovative technique, the good technical effect is realized, the cost is relatively low, the implementation is easy, and the good popularization prospect is good.

The invention discloses a radix tetrastigme compound composite for enhancing anti-tumor and antioxidant activity with radix tetrastigme as a main body, and a preparation method of the radix tetrastigme compound composite. The radix tetrastigme compound composite is prepared from the radix tetrastigme, spica prunellae, curcuma zedoary, herba agrimoniae and L-methyl selenocysteine. By using the compound product involved in the invention, a vast majority of active components in an extract are released advantageously, the effects of prevention and auxiliary treatment of tumors, improvement of antioxidant activity and immunity and the like can be provided for healthy population and cancer patients, the great benefit is achieved especially for auxiliary treatment and rehabilitation of the cancerpatients, the toxic and side effects of radiotherapy and chemotherapy can be eliminated, immunity is enhanced, and the radix tetrastigme compound composite is also suitable for tumor prevention and improvement of the antioxidant activity of bodies of ordinary people.

The invention discloses a preparation method of composition for inhibiting cicatricial healing of wound. The method includes the following steps of (1) weighing L-selenium-methyl selenocysteine and hyaluronic acid according to the ratio, respectively and measuring ultrapure water according to the ratio, dissolving the weighed L-selenium-methyl selenocysteine into the ultrapure water and conductingstirring, conducting shearing, conducting shaking or conducting vortexing by an ultrasonic mixer until the L-selenium-methyl selenocysteine is completely dissolved in the ultrapure water, and then adding the weighed hyaluronic acid and conducting swelling at room temperature for 1-2 days to obtain a composition stock solution; (2) Passing the composition stock through a 0.22 mum micro-porous filter under sterile conditions, conducting packaging under sterile conditions after sterilization, and conducting storing by placing the solution at 2-8 DEG C to obtain the composition for inhibiting cicatricial healing of the wound. The composition for inhibiting cicatricial healing of the wound prepared by the invention can effectively reduce cicatricial healing of the wound, and can reduce pigmentation of the wound site at the stage of wound shaping.

The invention provides health food for promoting eliminating of lead and a preparation method of the health food. The health food contains main materials and auxiliary materials, wherein the main materials include stachyose, zinc gluconate, vitamin C, taurine and L-selenium-methyl selenocysteine; and the auxiliary materials include lactose, D-mannitol, strawberry fruit powder, magnesium stearate, a film coating agent, a sweetener and edible flavors. The health food has a comprehensive formula constitution, the stachyose is capable of reducing absorption of lead, shortening standing time of lead in a human body and enhancing antagonism among zinc, selenium and lead, the taurine is capable of simultaneously maintaining osmotic balance in the human body and reducing the harm of lipid peroxidation of lead together with selenium, and vitamin C is capable of simultaneously decreasing lead content in blood and promoting the discharging of lead from different channels; and L-selenium-methyl selenocysteine in the formula is a novel selenium source and is capable of effectively entering the human body through absorption, improving lipid peroxidation reaction induced by lead poisoning and relieving the harm of lead, and a generated lead-selenium-protein composite can be discharged from different channels.

The invention provides a whitening and freckle-removing cosmetic composition containing lipidosome and application thereof, the composition contains nanoscale hyaluronic acid, L-selenium-methyl selenocysteine, ascorbic acid and analogues thereof, and is more easily absorbed and utilized by skin cells; deep moisturizing and nutrition supplementing of the skin can be effectively achieved, stains are effectively faded, the skin is whiter, and skin pigmentation and skin quality are improved; no harmful chemical component is added in the used composition formula, the quality is strictly controlled according to the formula components, medicinal-grade components are used, harmful substances are prevented from being mixed in from the source, and the composition is safer and healthier to use.

The invention relates to selenium-rich nutrition recombination rice. The nutrition rice is prepared from the following raw materials in parts by mass: 40-60 parts of selenium-rich corn meal, 40-60 parts of high-selenium-rich rice bran powder, 1-5 parts of high-selenium-rich volvariella volvacea powder, 1-10 parts of black sesame seed powder, 1-10 parts of soybean protein isolate and L-selenium-methyl selenocysteine. The raw materials are sufficiently mixed and then are blended with a proper amount of water; low-temperature controlled gelatinization granulation by a twin screw extruder and drying are performed to obtain recombinant nutrition pre-mixed artificial rice. By mixing the selenium-rich nutrition recombination pre-mixed rice prepared by the technology with common rice according toa ratio of 1 to 4, a product meeting the requirement on the selenium content of selenium-rich rice and having high absorption rate, metabolic rate and utilization rate can be prepared. The invention effectively solves the problems that the country has limited resources in selenium-rich regions, the raw materials of natural selenium-rich food are of excessive difference in selenium content and someof the food raw materials contain too much selenium; meanwhile, the rice bran byproduct in the processing of selenium-rich rice is scientifically utilized.

The invention discloses a special fertilizer for selenium-rich rice cultivation, and a preparation method thereof. The special fertilizer for selenium-rich rice cultivation is applied in the filling period of rice according to an application amount of 35-40 kg/666.7 m<2>; and the special fertilizer for selenium-rich rice cultivation comprises, by weight, 21-30 parts of selenium-rich red dates, 15-25 parts of selenium-rich fish meal, 5-10 parts of glucolactone, 10-16 parts of urea, 5-15 parts of selenium-rich yeast powder, 10-15 parts of L-selenium-methyl selenocysteine, 6-15 parts of boric acid, 7-15 parts of zinc sulfate, 8-15 parts of humic acid, 6-15 parts of diammonium phosphate and 10-22 parts of dried small shrimp powder. The special fertilizer for selenium-rich rice cultivation canbe applied in the filling period of rice, can increase the selenium content of the rice by 25% or above, also can improve the yield, taste and nutritional values of the rice, and has good applicationand promotion values.

The invention discloses selenium-rich health astragalus smicus scented tea and a preparing method thereof. The scented tea is prepared from astragalus smicus flower, jasmine flower and red dates. The weight ratio of components of astragalus smicus flower, jasmine flower and red dates in the scented tea is (6-8.5):(1-3.5):0.5. The selenium content of the selenium-rich health astragalus smicus scented tea which is provided by the invention can reach 50-6000 mg/kg, the organic selenium content is greater than or equal to 80%, and selenium mainly exists in the forms of selenomethionine, methyl selenocysteine and selenocystine. The selenium-rich health astragalus smicus scented tea is a great selenium replenishing product and meanwhile has the effects of dispelling wind, brightening the eye, invigorating the spleen, benefiting qi, relieving toxicity, alleviating pain and the like.

The invention discloses a method for determining the form content of selenium in vegetables. According to the method, seven selenium forms and contents of selenocystine, methyl selenocysteine, selenourea, selenomethionine, selenoethionine, selenite radicals and selenate radicals in a sample to be detected are tested by using an ion pair reversed-phase high-performance liquid chromatography-ultraviolet-hydride generation-atomic fluorescence spectrometry. According to the determination method disclosed by the invention, iodoacetamide is added in inorganic selenium extraction, so a problem of poor recovery rate of selenite radical analysis and test in HPLC-HG-AFS is solved; pretreatment operation is fast and simple, the required types and dosage of protease are few, reagent cost is low, the sample extraction rate is high, and sensitivity and the separation degree of the selenium form HPLC-HG-AFS analysis method are greatly improved; contents of seven selenium forms can be simultaneously analyzed and determined at one time, and the instrument analysis and test time is short.

The invention provides an enzymatic synthesis method for L-selenium-methyl selenocysteine. In particular, L-selenocysteine and S-methyl-L-methionine are taken as substrates, and immobilized selenocysteine-selenium methyltransferase (EC 2.1.1.280) is taken as a catalyst to synthesize L-selenium-methyl selenocysteine in a continuous or batch process. The product can be taken as an organic selenium food additive or a supplement additive after purification. A synthesis reaction is performed under a mild condition in an environment-friendly way, and has the characteristics of specificity, high efficiency, simple steps, easiness in control, no production of harmful impurities in the reaction and low substrate cost. The enzymatic synthesis method is suitable for producing the L-selenium-methyl selenocysteine in an environment-friendly and low-cost manner.