Method for determining selenium form content in vegetables

A form and content technology, applied in the field of determination of selenium form content in vegetables, can solve the problems of poor selenium detection sensitivity and long analysis time, and achieve the effects of low reagent cost, high sample extraction rate, and short instrument analysis test time
CN113189233AActive Publication Date: 2021-07-30INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
Publication Date
2021-07-30

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Abstract

The invention discloses a method for determining the form content of selenium in vegetables. According to the method, seven selenium forms and contents of selenocystine, methyl selenocysteine, selenourea, selenomethionine, selenoethionine, selenite radicals and selenate radicals in a sample to be detected are tested by using an ion pair reversed-phase high-performance liquid chromatography-ultraviolet-hydride generation-atomic fluorescence spectrometry. According to the determination method disclosed by the invention, iodoacetamide is added in inorganic selenium extraction, so a problem of poor recovery rate of selenite radical analysis and test in HPLC-HG-AFS is solved; pretreatment operation is fast and simple, the required types and dosage of protease are few, reagent cost is low, the sample extraction rate is high, and sensitivity and the separation degree of the selenium form HPLC-HG-AFS analysis method are greatly improved; contents of seven selenium forms can be simultaneously analyzed and determined at one time, and the instrument analysis and test time is short.
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Description

technical field

[0001] The invention belongs to the technical field of quality inspection, and in particular relates to a method for measuring the content of selenium form in vegetables. Background technique

[0002] Selenium, as an essential nutrient element for the human body and an important component of various active enzymes in the human body, has been designated by the World Health Organization and the Chinese Medical Association as the third most essential micronutrient health element after iodine and zinc in the 21st century . Selenium plays an important role in preventing Keshan disease, enhancing immunity, anti-oxidation, anti-cancer, protecting the liver, protecting the heart, promoting mental development, preventing and treating diabetes, regulating endocrine and vitamin absorption and utilization, and protecting the thyroid and prostate. effect.

[0003] The bioavailability, effectiveness, toxicity and metabolism of selenium in organisms are not only related t...

Claims

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