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Method for determining selenium form content in vegetables

A form and content technology, applied in the field of determination of selenium form content in vegetables, can solve the problems of poor selenium detection sensitivity and long analysis time, and achieve the effects of low reagent cost, high sample extraction rate, and short instrument analysis test time

Active Publication Date: 2021-07-30
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005]Therefore, the technical problem to be solved in the present invention is that in the prior art, the form and content detection sensitivity of selenium in vegetables is poor, and the analysis time is too long, so as to provide A method for determining the content of selenium in vegetables, specifically ion-pair reversed-phase high performance liquid chromatography-ultraviolet-hydride generation-atomic fluorescence spectrometry (IP-RP-HPLC-UV-HG-AFS)

Method used

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  • Method for determining selenium form content in vegetables
  • Method for determining selenium form content in vegetables
  • Method for determining selenium form content in vegetables

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Embodiment 1

[0033] The instrument used in embodiment 1 and embodiment 2 is HPLC-UV-HG-AFS (SA-20, Beijing Jitian Instrument Co., Ltd.) and analysis conditions are as follows:

[0034] Liquid chromatography: C18 reverse-phase chromatographic column (250×4.6mm, 5μm), the mobile phase is 30mmol / L diammonium hydrogen phosphate solution, which contains 0.5mmmol / L tetrabutylammonium bromide, and also contains 2% methanol, pH is 6.0, the flow rate is 1.2mL / min, the injection volume is 100μL, and the column temperature is 35°C;

[0035] Ultraviolet: online ultraviolet digestion (ultraviolet lamp power 40w, length 28cm);

[0036] Hydride generation: Conditions: 0.35% (m / v) potassium hydroxide + 0.05% (m / v) potassium iodide, 0.35% (m / v) potassium hydroxide + 1.5% (m / v) potassium borohydride, 10% (v / v) hydrochloric acid, pump speed 60r / min;

[0037] Atomic fluorescence spectroscopy: negative high voltage 300V, total lamp current 80mA, carrier gas flow 300mL / min, shielding gas flow 500mL / min, atomi...

Embodiment 2

[0050] The present embodiment provides a method for measuring the content of selenium in vegetables. The sample to be tested is selenium-enriched water spinach spinach (the variety is No. 1 Ganshui), and the specific steps are as follows:

[0051] (1) Wash the spinach water spinach rich in selenium with water, dry it, take about 200 g of the edible part, and homogenize it with a homogenizer for 1 min to obtain a processed sample to be tested;

[0052] (2) Take 2g of the sample to be tested in step (1) in a 50mL centrifuge tube, add 5.0mL of water and 0.1mL of 100mmol / L iodoacetamide solution, shake well, ultrasonically extract in an ultrasonic instrument at a frequency of 20kHz for 30min, repeat 2 The second time, the extract is filtered through quantitative filter paper, and the volume is adjusted to a 10mL volumetric flask with water. Take 5mL of the filtrate in an ultrafiltration centrifuge tube, and centrifuge and purify at 5000rpm for 20min. ;At the same time, do a blank ...

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Abstract

The invention discloses a method for determining the form content of selenium in vegetables. According to the method, seven selenium forms and contents of selenocystine, methyl selenocysteine, selenourea, selenomethionine, selenoethionine, selenite radicals and selenate radicals in a sample to be detected are tested by using an ion pair reversed-phase high-performance liquid chromatography-ultraviolet-hydride generation-atomic fluorescence spectrometry. According to the determination method disclosed by the invention, iodoacetamide is added in inorganic selenium extraction, so a problem of poor recovery rate of selenite radical analysis and test in HPLC-HG-AFS is solved; pretreatment operation is fast and simple, the required types and dosage of protease are few, reagent cost is low, the sample extraction rate is high, and sensitivity and the separation degree of the selenium form HPLC-HG-AFS analysis method are greatly improved; contents of seven selenium forms can be simultaneously analyzed and determined at one time, and the instrument analysis and test time is short.

Description

technical field [0001] The invention belongs to the technical field of quality inspection, and in particular relates to a method for measuring the content of selenium form in vegetables. Background technique [0002] Selenium, as an essential nutrient element for the human body and an important component of various active enzymes in the human body, has been designated by the World Health Organization and the Chinese Medical Association as the third most essential micronutrient health element after iodine and zinc in the 21st century . Selenium plays an important role in preventing Keshan disease, enhancing immunity, anti-oxidation, anti-cancer, protecting the liver, protecting the heart, promoting mental development, preventing and treating diabetes, regulating endocrine and vitamin absorption and utilization, and protecting the thyroid and prostate. effect. [0003] The bioavailability, effectiveness, toxicity and metabolism of selenium in organisms are not only related t...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/14G01N30/30G01N30/32G01N30/34G01N30/74G01N30/86G01N21/64
CPCG01N30/02G01N30/14G01N30/30G01N30/32G01N30/34G01N30/74G01N30/8675G01N21/6404G01N2030/146G01N2030/324
Inventor 魏益华张金艳戴廷灿邱素艳涂田华
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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