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Culture medium for promoting growth of scenedesmus and accumulation of lutein

A medium and lutein technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low biomass lutein content, failure to meet industrialization standards, etc., and achieve improved biological The effect of improving the comprehensive utilization value

Inactive Publication Date: 2019-08-02
JIANGSU QIANGNONG AGRI TECHNICAL SERVICES CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the production of lutein by Scenedesmus is still in its infancy in our country, and under the existing technical conditions, the biomass and lutein content of Scenedesmus culture are at a low level, which has not yet reached the level of lutein. Industrialization standard

Method used

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  • Culture medium for promoting growth of scenedesmus and accumulation of lutein
  • Culture medium for promoting growth of scenedesmus and accumulation of lutein
  • Culture medium for promoting growth of scenedesmus and accumulation of lutein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Effects of Carbon and Nitrogen Mass Ratio on Scenedesmus Growth and Lutein Content

[0034] Prepare medium according to the following ratio components: NaNO 3 135mg / L, soybean peptone 65mg / L, KH 2 PO 4 50mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 37mg / L, citric acid 6.1mg / L, ferric ammonium citrate 4.2mg / L, Na 2 EDTA5.2mg / L, H 3 BO 3 2.9mg / L, MnSO 4 4H 2 O 0.68mg / L, ZnSO 4 ·7H 2 O 0.23mg / L, CuSO 4 ·5H 2 O0.015mg / L, Na 2 MoO 4 2H 2 O 0.24mg / L, Co(NO 3 ) 2 ·6H 2 O 0.015mg / L, inositol 90mg / L, thiamine hydrochloride 1.0mg / L, pantothenic acid 0.05mg / L, folic acid 0.05mg / L, biotin 0.02mg / L, valine 7.5mg / L, leucine Acid 12.5mg / L, calcium diphenylureasulfonate 1.5mg / L, IAA 0.7mg / L, α-ketoglutaric acid 3.0mg / L, clethodim 12μg / L, nicotine 0.06mg / L, spinach Juice 5ml / L, pH value is 7.0.

[0035] Add 2, 3, 4, 5, 6 g / L of glucose to the culture medium respectively, so that the mass ratio of carbon to nitrogen is 10:1, 15:1, 20:1, 25:1 and 30:1 respe...

Embodiment 2

[0037] Example 2: Effect of Calcium Diphenylureasulfonate Concentration on Growth of Scenedesmus and Lutein Content

[0038] Prepare the medium according to the following ratio components: glucose 5g / L, NaNO 3 135mg / L, soybean peptone 65mg / L, K 2 HPO 4 50mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 37mg / L, citric acid 6.1mg / L, ferric ammonium citrate 4.2mg / L, Na 2 EDTA 5.2mg / L,H 3 BO 3 2.9mg / L, MnSO 4 4H 2 O 0.68mg / L, ZnSO 4 ·7H 2 O 0.23mg / L, CuSO 4 ·5H 2 O 0.015mg / L, Na 2 MoO 4 2H 2 O 0.24mg / L, Co(NO 3 ) 2 ·6H 2 O 0.015mg / L, inositol 90mg / L, thiamine hydrochloride 1.0mg / L, pantothenic acid 0.05mg / L, folic acid 0.05mg / L, biotin 0.02mg / L, valine 7.5mg / L, leucine Acid 12.5mg / L, IAA 0.7mg / L, α-ketoglutaric acid 3.0mg / L, clethodim 12μg / L, nicotine 0.06mg / L, spinach juice 5ml / L, pH value 7.0.

[0039] Add the calcium diphenylureasulfonate of 0, 0.5, 1.0, 1.5, 2.0 mg / L respectively in the culture medium, measure the biomass and lutein content of Scenedesmus after...

Embodiment 3

[0041] Example 3: Effects of α-ketoglutarate concentration on growth of Scenedesmus and lutein content

[0042] Prepare the medium according to the following ratio components: glucose 5g / L, NaNO 3 135mg / L, soybean peptone 65mg / L, K 2 HPO 4 50mg / L, MgSO 4 ·7H 2 O 75mg / L, CaCl 2 2H 2 O 37mg / L, citric acid 6.1mg / L, ferric ammonium citrate 4.2mg / L, Na 2 EDTA 5.2mg / L,H 3 BO 3 2.9mg / L, MnSO 4 4H 2 O 0.68mg / L, ZnSO 4 ·7H 2 O 0.23mg / L, CuSO 4 ·5H 2 O 0.015mg / L, Na 2 MoO 4 2H 2 O 0.24mg / L, Co(NO 3 ) 2 ·6H 2 O 0.015mg / L, inositol 90mg / L, thiamine hydrochloride 1.0mg / L, pantothenic acid 0.05mg / L, folic acid 0.05mg / L, biotin 0.02mg / L, valine 7.5mg / L, leucine Acid 12.5mg / L, calcium diphenylureasulfonate 1.5mg / L, IAA 0.7mg / L, clethodim 12μg / L, nicotine 0.06mg / L, spinach juice 5ml / L, pH 7.0.

[0043] Add α-ketoglutaric acid with a concentration of 0, 1.0, 3.0, 5.0, and 7.0 mg / L to the culture medium, measure the biomass and lutein content of Scenedesmus after the cultur...

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Abstract

The invention discloses a culture medium for promoting growth of scenedesmus and accumulation of lutein. The culture medium comprises a carbon source, a nitrogen source, phosphate, a calcium salt, aniron salt, a plurality of trace elements and vitamins according to a specific proportional ratio, and also comprises valine, leucine, L-selenium-methyl selenocysteine, silver thiosulfate, diphenylureasulfonic calcium, IAA (indoleacetic acid), alpha-ketoglutaric acid, clethodim, nicotine and spinach juice. The culture medium disclosed by the invention has the advantages that by optimizing the carbon source and the nitrogen source, and adding a growth regulator, a precursor compound synthesized and metabolized by the lutein, and a plurality of means for facilitating the accumulation of the lutein, the biomass of the scenedesmus is obviously increased, and the synthesizing and accumulation of the lutein are also obviously increased; by adopting the innovative technique, the good technical effect is realized, the cost is relatively low, the implementation is easy, and the good popularization prospect is good.

Description

technical field [0001] The invention belongs to the technical field of microalgae cultivation, and in particular relates to a culture medium for promoting the growth of Scenedesmus and the accumulation of lutein. Background technique [0002] Lutein, also known as plant lutein, riboflavin, marigold flower element, etc., is a class of natural carotenoids, widely found in flowers, fruits, vegetables and algae. Lutein co-exists with zeaxanthin in nature and is the main component of plant pigments. Therefore, it can be used as a natural pigment for food, medicine and cosmetic pigment additives, as well as color enhancers for poultry and animal tissues. Lutein is also an antioxidant with excellent performance and can absorb harmful rays such as blue light. As early as 1995, the US Food and Drug Administration (FDA) had approved it as a food supplement for food and beverage. Lutein is the only carotenoid that exists in the retina of the human eye. It is selectively deposited in t...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 杨星宇应向军丁鼎平
Owner JIANGSU QIANGNONG AGRI TECHNICAL SERVICES CO LTD
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