25 results about "Scenedesmus" patented technology

The invention relates to a soil conditioner for improving stability of organic matters in brick red soil and application of the soil conditioner. The soil conditioner comprises microalgae, humic acid or derivatives thereof. The microalgae comprise scenedesmus and / or chlorella. According to the soil conditioner developed by the invention, scenedesmus and / or chlorella are / is taken as a main raw material, humic acid or a derivative thereof is taken as an auxiliary material, and the scenedesmus and the chlorella are matched together, so that the soil conditioner has excellent effects of promoting formation of soil microaggregates and promoting formation of large aggregates, and further has an excellent effect of improving the stability of brick red soil organic matters.

The invention relates to a method for improving nitrogen and phosphorus utilization efficiency of brick red soil and application of the method. The method comprises the following steps: applying microalgae cell sap to the brick red soil; the microalgae comprise scenedesmus and / or chlorella. The brand-new method strategy for improving the nitrogen and phosphorus utilization efficiency of the brick red soil is developed, operation and implementation of the method strategy are quite easy, the method strategy is suitable for planting the brick red soil of various crops, and the effect of improving the nitrogen and phosphorus utilization efficiency of the brick red soil and the crop yield is quite remarkable.

The invention relates to the technical field of oil field reservoir damage inhibition, in particular to a carbon quantum dot biological composite material and a preparation method and application thereof. The preparation method of the carbon quantum dot biological composite material provided by the invention comprises the following steps: mixing a scenedesmus extracting solution with an aqueous dispersion of carbon quantum dots, then adjusting the pH value to 8-10, and carrying out a grafting reaction to obtain the carbon quantum dot biological composite material, the scenedesmus extracting solution is a mixed solution of intracellular substances released after scenedesmus cells are broken and water. The carbon quantum dot biological composite material prepared by the method can be used as an asphaltene deposition inhibitor, has the characteristics of greenness, high efficiency and low cost, and can effectively inhibit asphaltene deposition, so that the fluidity of crude oil is improved, reservoir damage is reduced, and the production cost is reduced.

The present invention discloses a continuous extraction method for microalgae active ingredients, comprising the following steps: after harvesting Scenedesmus, hot water extraction is carried out at a hot water temperature of 75-90 °C, the supernatant and algal sludge A are collected, the supernatant is subjected to alcohol precipitation to obtain crude polysaccharide; anhydrous methanol is added to algal sludge A, and ultrasonic extraction is carried out, the supernatant and algal sludge B are respectively collected, and the supernatant is the methanol extract; a chloroform and methanol mixed solution with a volume ratio of chloroform to methanol of 1.5-2.5:1 is added to algal sludge B, ultrasonic-assisted extraction is carried out, and the supernatant is collected to obtain crude lipid. This method can simultaneously improve the extraction rate of the methanol extract and the content of neutral lipids in the crude lipid, and the total amount of the extract can reach 39%, which can effectively reduce the remaining amount of algal residue after extraction, providing a new method for the application of microalgae biomass.

This invention relates to a method for increasing the biomass of *Scenedesmus* under light and its application. The method includes the following steps: inoculating *Scenedesmus* stock solution into a sterile liquid culture medium, and culturing the *Scenedesmus* under light using a composite spectrum of white and red light. This invention develops a novel method for culturing *Scenedesmus*, namely, culturing the *Scenedesmus* under light using a composite spectrum of white and red light. This method is simple to operate and easy to implement. It can significantly improve the growth rate and biomass accumulation of *Scenedesmus*, enabling high-quality, large-scale production; it can also improve light and electrical energy utilization efficiency, reduce energy costs, and solve the technical challenge of simultaneously increasing production and reducing energy consumption in *Scenedesmus* cultivation.

The present disclosure relates to a biodegradable geotextile structure for soil biostimulation comprising a woven fabric structure of hydrophilic filament yarns of a cellulose fiber selected from the list consisting of: alpha cellulose, beta cellulose, gamma cellulose or mixtures thereof; at least a plant-based biopesticide selected from the list consisting of: Nicotine, Rotenone, Pyrethrins I, Pyrethrins II, Eugenol, Citronella oil, and mixtures thereof; at least a microalgae selected from the list consisting of: Chlorella spp., Scenedesmus spp, Dunaliella spp, Spirulina spp., and mixtures thereof. Furthermore, the present disclosure relates to a method to obtain said biodegradable geotextile structure.

The present invention discloses a method for synergistically treating environmental cadmium pollution by using root secretions of aquatic plants and microalgae, comprising the following steps: (1) preparation of root secretions of aquatic plants: washing the aquatic plants, placing them in a container filled with pure water, fixing the roots with clean sand and gravel, and culturing the roots; then taking out the water in the container, filtering the roots, and obtaining a solution of root secretions of aquatic plants; (2) adsorption treatment of cadmium pollutants: adding the root secretion solution of aquatic plants and microalgae to the cadmium pollutants, and carrying out culturing and adsorption treatment. The present invention selects root secretions of four aquatic plants, namely, Vallisneria, Water Cabbage, Water Lily, and Water Hyacinth, to synergistically treat environmental cadmium pollution with Microcystis aeruginosa, Chlorella, Chrococcus, and Scenedesmus, which can effectively improve the absorption of Cd by microalgae. 2+ The adsorption capacity of the product is of great significance to the treatment of environmental pollution.

The invention belongs to the technical field of biological wastewater treatment, and particularly relates to scenedesmus F1 and application thereof, scenedesmus liquid and a culture method and application thereof, and a treatment method of nitrogen-containing wastewater. The invention provides a scenedesmus sp. F1, and the preservation number of the scenedesmus sp. F1 is CCTCC (China Center For Type Culture Collection) NO: M20242540. The scenedesmus F1 provided by the invention can digest and remove nitrogen in the nitrogen-containing wastewater, and has the advantages of rapid growth and relatively strong adaptability under the condition of relatively high nutrient concentration, so that efficient removal of nitrogen in the nitrogen-containing wastewater is realized; in addition, the scenedesmus F1 also has the advantage of rapid sedimentation, can reduce the energy consumption in the biomass collection process, reduces the overall treatment cost, and has wide application prospects in industrial wastewater treatment.

The invention belongs to the field of sewage treatment, and particularly relates to an A / O-MPBR system and a construction method and application thereof. According to the specific technical scheme, the sewage treatment system comprises an A / O treatment system and an MPBR system which are communicated in sequence; the A / O system and the MPBR are mutually independent and communicated in space, and sewage firstly flows through the A / O system to be treated and then enters the MPBR to be treated. And the algae inoculated in the MPBR is Scenedesmus alliensis (Scenedesmus alliensis). The method is characterized in that the algae inoculated in the MPBR are in the form of Scenedesmus alliensis. The invention constructs a microalgae-membrane photosynthetic reactor system capable of continuously feeding and discharging water, and further optimizes operation conditions and screens out microalgae with low nutrition tolerance aiming at the low nutrition background of A / O sewage treatment effluent. The tolerant microalgae and membrane combined photosynthetic reactor is combined for use and is coupled with pilot-scale A / O sewage treatment equipment for operation, so that pollutants are further removed, and the synergistic removal efficiency of antibiotic resistance genes (ARGs) is also remarkably improved.

The invention relates to a biomass energy utilization technology, and aims to provide a method for promoting scenedesmus growth and carbon sequestration by using polyacrylamide in oilfield wastewater. Comprising the following steps: inoculating an original scenedesmus strain into a photobioreactor filled with a liquid culture medium, providing a proper illumination condition, and introducing CO2 / N2 mixed gas into the reactor; the concentration of polyacrylamide in the liquid culture medium is increased in a gradient increasing mode, and inoculation is repeated multiple times till domestication is completed; scenedesmus strains capable of stably inheriting are selected for enlarged culture, oilfield wastewater containing polyacrylamide is used for preparing a liquid culture medium, and decomposition of polyacrylamide is promoted while carbon sequestration growth of scenedesmus is achieved. The polyacrylamide in the oilfield wastewater is utilized to promote the growth and carbon sequestration of the scenedesmus, the polyacrylamide can be degraded while the carbon sequestration growth is realized, the double results of carbon sequestration and waste removal are realized, and the comprehensive benefit of the method is far better than that of a fungus waste removal scheme in the traditional technology.

The invention relates to the technical field of photodynamic therapy, in particular to a synthesis method of an algae integrated oxygen supply platform based on OLED photoelectric shielding material modification, and the method comprises the following steps: step 1, selecting a photoelectric material based on pentaperylene diimide (PPD), including PPD derivatives modified by PPD-Se, PPD-Te and PPD-Br and containing heavy atoms as a core photosensitizer; step 2, mixing a photoelectric material based on PPD, a PEG derivative containing PPD-Se and a responsive chemical bond, and DSPE-PEG-S-S-NHH through a coprecipitation method, and stirring for 1-6 hours at the temperature of 30-70 DEG C to form PPD type photoelectric material nano-particles with the particle size within the range of 80-120 nm, namely PPD-based NPs; step three, pretreatment and compounding of green algae: chlorella, scenedesmus and chlamydomonas are used as oxygen supply carriers, the surface of the green algae is modified through carboxymethyl chitosan CMCS, and carboxyl groups are introduced; and step 4, combining the modified PPD type photoelectric material nano particles with the microalgae through amido bonds, so as to form the microalgae-photoelectric material composite system Alga (at) PPD-based.

This invention relates to a novel microalgae with high ryegrass lactone production. The *Scenedesmus* HS4 of this invention has high biomass production and high ryegrass lactone content, and therefore can be used as a biological resource for producing ryegrass lactone. It can be used in pharmaceutical or cosmetic compositions that require ryegrass lactone derived from *Scenedesmus* HS4.

The invention discloses a fungal inoculant and application thereof in algae crust, and belongs to the field of microorganisms. The technical problem to be solved by the invention is how to construct the algae crust. The application of the fungal inoculant in the algal crust comprises the following steps: inoculating deep-color endophytic fungi and microalgae to the surface layer of soil to be remediated, and culturing to realize the construction of the algal crust; the deep-color endophytic fungi are bacterial strains with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.17463 in the China General Microbiological Culture Collection Center; the microalgae is composed of Microcoleus sp., Scenedesmus sp. And Chlorella sp., and the microcoleus sp., the Scenedesmus sp. And the Chlorella sp. Are used in the method. According to the method, rapid formation of algae crust and improvement of soil carbon accumulation are successfully realized, the key technical problems of long crust period, poor adaptability and the like are solved, and the purpose of ecological restoration of degraded soil in an arid and semi-arid mining area is favorably realized.

The invention discloses a scenedesmus growth prediction method and system based on multi-source data fusion and a medium, and belongs to the field of data processing, and the method comprises the following steps: obtaining multi-source data of scenedesmus; performing quantification processing on the environmental stress data to obtain a microalgae stress inhibition coefficient, and constructing an environmental stress vector according to the microalgae stress inhibition coefficient and the environmental stress data; performing quantitative processing on the biological effect data to obtain a microalgae stress inhibition coefficient, and then determining a total effect coefficient; constructing a growth environment vector according to the growth environment data, and constructing a biological effect vector according to the biological effect data and the total effect coefficient; fusing the growth environment vector, the biological effect vector and the environmental stress vector to obtain a fusion vector; the real growth data and the real survival rate corresponding to the multi-source data of scenedesmus are obtained to construct a training set, and then a preset deep learning network model is trained to obtain a trained model. According to the invention, the prediction precision of scenedesmus growth is improved.

The present invention relates to novel microalgae with high productivity of loliolide, in which the Scenedesmus sp. HS4 of the present invention can be used as a biological resource to produce loliolide due to its high biomass productivity and high loliolide content, and can be used as a pharmaceutical composition or cosmetic composition which requires loliolide derived from Scenedesmus sp. HS4.

The invention belongs to the technical field of wastewater treatment and biological energy production, and provides scenedesmus Fs and application thereof, scenedesmus liquid and a culture method and application thereof, and a phosphorus-containing wastewater treatment method. The invention provides a Scenedesmus sp. Fs (Scenedesmus sp.), and the preservation number of the Scenedesmus sp. Fs is CCTCC (China Center for Type Culture Collection) NO: M20242541. The scenedesmus Fs provided by the invention can effectively remove phosphorus in wastewater; meanwhile, the scenedesmus Fs is high in lipid content and has potential application in biodiesel production.

This invention discloses a deep learning-based method and related equipment for locating and counting cells in *Scenedesmus* (a type of algae). The method first acquires microscopic images of *Scenedesmus* populations and labels the cell locations to form a set of labeled cell points. Then, a *Scenedesmus* cell counting network is constructed, including a backbone network, density branches, and regression branches, to extract features from the images and model the spatial distribution of cells. Enhanced images are generated by preprocessing the microscopic images, and the network is trained using the labeled information as supervision. After model training, new microscopic images are input into the network to obtain initial cell location and counting results. Furthermore, by incorporating the biological prior constraint that the number of cells in a *Scenedesmus* population is even, the initial counting results are post-processed and corrected, finally outputting the cell location and counting results. This invention enables automatic location and accurate counting of cells in *Scenedesmus* populations, exhibiting high recognition accuracy and application value.

The invention relates to the field of biological treatment of nitrogen and phosphorus in aquaculture wastewater, in particular to a Scenedesmus euryales strain and application thereof in degradation of nitrogen and phosphorus in aquaculture wastewater. The scenedesmus is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.46073, and the preservation date is August 12, 2024. The scenedesmus obtained through separation and screening can well adapt to the euryhaline wastewater after being subjected to salinity adaptability domestication, is stable in growth and has the application potential in the aspect of removing nitrogen and phosphorus in the mariculture wastewater.

The present invention relates to a method for improving nitrogen and phosphorus utilization efficiency in red soil and its application. The method comprises applying microalgae cell sap to the red soil; the microalgae include Scenedesmus and / or Chlorella. The present invention develops a novel method and strategy for improving nitrogen and phosphorus utilization efficiency in red soil. This method and strategy is simple to implement and is suitable for cultivating various crops in red soil. It also significantly improves nitrogen and phosphorus utilization efficiency in red soil and crop yields.

The invention discloses a preparation method of scenedesmus liquid mediated synthesized carbon quantum dot nanofluid and an oil displacement agent. The preparation method comprises the following steps: preparing scenedesmus liquid; the preparation method comprises the following steps: preparing carbon quantum dots, taking glacial acetic acid as a carbon source, and reacting phosphorus pentoxide with glacial acetic acid to release heat to form nano C-DOTs; the preparation method comprises the following steps: adding pure water into nano C-DOTs, magnetically heating and stirring at 70 DEG C, and then adding scenedesmus liquid and a proper amount of NH3.H2O to obtain the scenedesmus liquid-mediated synthesized carbon quantum dot nanofluid. According to the preparation method and the oil-displacing agent, the nanofluid is obtained by adopting green bio-based liquid, and small organic molecules generated by organisms have the effect of stably dispersing nano particles; the biological nanofluid has environmental friendliness and efficient oil displacement potential.

The invention discloses a microbial inspection method based on marine biological medicines, and belongs to the technical field of marine biological medicines. The method comprises the following steps: firstly, researching a specific molecular marker of nannochloropsis oculata, and comparing the specific molecular marker with amino acid sequences of other marine algae to determine a highly stable protein fragment special for nannochloropsis oculata; on the basis, the nannochloropsis oculata in the water sample can be efficiently detected through an amino acid sequencing method. The method not only can be used for identifying the nannochloropsis oculata from the other algae such as the balea, the scenedesmus, the hornwort mulleri, the benthic navicula and the like, but also can realize quantitative detection of the nannochloropsis oculata. Compared with a traditional classification and identification technology, the method has the advantages that the accuracy is higher, and the application range of samples is wider; compared with a PCR (Polymerase Chain Reaction) detection method, the method is simple and convenient to operate, lower in cost, quicker and more sensitive.

The invention discloses application of scenedesmus in amorphophallus bulbifer planting, and belongs to the technical field of amorphophallus bulbifer planting. In the planting process of the amorphophallus bulbifer, scenedesmus liquid with the biomass of 3-4 g / L is applied once a week, the application amount is 1.4-1.5 ml / m < 2 >, growth of the amorphophallus bulbifer can be promoted, and absorption of cadmium by the amorphophallus bulbifer can be reduced. The method is efficient and easy to operate, and a new technical method is provided for promoting growth of the amorphophallus bulbifer and inhibiting absorption of cadmium by the amorphophallus bulbifer.

The invention discloses a Z-shaped sewage removal device for microalgae co-culture, and belongs to the technical field of sewage treatment devices. According to the method, the characteristic that scenedesmus and chlorella are co-cultured to synergistically promote nutrition utilization and algae growth under a severe environment condition is utilized, carbon dioxide in sewage is absorbed through photosynthesis under an illumination condition and is converted into organic substances, and nutrient substances such as ammonia nitrogen in the sewage are utilized for growth; furthermore, by constructing a non-immersed Z-shaped reactor, light can directly irradiate the microalgae attached to the surface of the cotton cloth, light attenuation caused by high turbidity of organic sewage in a farm is overcome, meanwhile, the landing area of the microalgae is increased, the removal capacity of a microalgae biological membrane is further improved, finally, ammonia nitrogen in the sewage is removed, and the sewage treatment efficiency is improved. And the microalgae biomass with economic value is also produced.

The application discloses a kind of fungal inoculant and its application in algae crust, belong to the field of microorganism.The technical problem solved by the application is how to construct algae crust.The application of fungal inoculant disclosed in the application in algae crust comprises the following steps: after dark endophytic fungi and microalgae are inoculated to the surface layer of soil to be repaired, culture is carried out, the construction of algae crust is realized;the dark endophytic fungi are the strain with the preservation number CGMCC No.17463 in China General Microbiological Culture Collection Center;the microalgae are composed of Microcoleus sp., Scenedesmus sp.and Chlorella sp.The application successfully realizes the rapid formation of algae crust, improves soil carbon accumulation, solves the key technical problems such as long crust period and poor adaptability, and is beneficial to the purpose of ecological restoration of degraded soil in arid and semiarid mining areas.

The invention belongs to the field of microorganisms, and discloses a strain of Scenedesmus sp. EMC7 separated from biological crust soil in a karst rocky desertification region, and the preservation number of the strain is CGMCC No.41200. The invention also discloses a preparation method of the Scenedesmus sp. The scenedesmus disclosed by the invention is high in growth and reproduction speed, and after the scenedesmus liquid prepared from the scenedesmus is applied to barren sandy soil and loam, the water-retaining property of the soil can be improved, the erosion resistance of the soil is enhanced, the nutrients of the soil are improved, and the growth and nutrient absorption of the cold-land bluegrass plants are promoted, so that the degraded land is effectively ecologically restored and treated, the cost is reduced, and the economic benefit is increased. Environmental pollution is reduced, and sustainable and healthy development of the ecological environment is achieved.