17 results about "Rotenone" patented technology

The invention discloses a construction method and application of an animal model of Parkinson's disease, and relates to the technical field of animal models of neurodegenerative diseases. The animal model of the Parkinson's disease induces alpha-synuclein to be transmitted along an intestine-brain axis. The construction method comprises the following steps: (1) providing an SD (Sprague Dawley) rat; and (2) performing intragastric administration on the SD rat with rotenone according to the dosage of 30mg / kg / day, so as to obtain the animal model of the Parkinson's disease. The Parkinson's disease modeling method successfully simulates sequential transmission of alpha-syn along the intestine-brain axis, has the advantages of strong targeting, low death rate, endogenous pathology generation and the like, and provides a reliable platform for PD mechanism research and treatment development. According to the screening method of the medicine for treating the Parkinson's disease, the blocking effect of the medicine on PD pathology source transmission can be accurately evaluated by detecting the deposition amount of alpha-syn at multiple parts, and the defects of a traditional model are overcome; the method is suitable for research and development requirements of various drugs, and the application range is far better than that of a screening model only aiming at a single pathological link.

The present disclosure relates to a biodegradable geotextile structure for soil biostimulation comprising a woven fabric structure of hydrophilic filament yarns of a cellulose fiber selected from the list consisting of: alpha cellulose, beta cellulose, gamma cellulose or mixtures thereof; at least a plant-based biopesticide selected from the list consisting of: Nicotine, Rotenone, Pyrethrins I, Pyrethrins II, Eugenol, Citronella oil, and mixtures thereof; at least a microalgae selected from the list consisting of: Chlorella spp., Scenedesmus spp, Dunaliella spp, Spirulina spp., and mixtures thereof. Furthermore, the present disclosure relates to a method to obtain said biodegradable geotextile structure.

The application relates to a microbial type rotenone biopesticide, which comprises the following raw materials in parts by weight: 70-90 parts of a microbial fermentation liquor, 200-250 parts of a rotenone solution and 250-300 parts of a mineral source potassium fulvate carrier, the mineral source potassium fulvate is wrapped with a chitosan protective film, the microbial fermentation liquor comprises bacillus thuringiensis, and the rotenone solution comprises 40-60 wt% rotenone. The application has the effects of prolonging the storage period of the microbial type biopesticide and stabilizing the control effect.

This invention belongs to the field of pesticide technology and discloses a synergistic ant-killing powder based on traditional Chinese medicine extracts. This powder uses aminated modified biochar as a bifunctional carrier, loading a complex of active ingredients including triptolide and rotenone. It addresses the problems of short duration of action and poor targeting of existing plant-derived ant killers through an electrostatic adsorption-slow-release synergistic mechanism. Its components include 65%–85% aminated modified biochar, 3%–8% triptolide, 2%–6% rotenone, 5%–15% flow aid, and 1%–3% anti-caking agent. The carrier is prepared from agricultural waste through oxygen-limited pyrolysis and silane grafting, possessing a high specific surface area and controllable pore size, which can stably anchor the active ingredients and trigger release in the alkaline microenvironment of ants. This ant-killing powder extends the duration of action, improves efficacy utilization, and has low toxicity to non-target organisms, making it suitable for green pest control in various scenarios.

This invention belongs to the field of medicinal chemistry, specifically relating to a mitochondrial-dequinoline chloride-hyaluronic acid compound, its preparation method, and its application. The rotenol-dequinoline chloride-hyaluronic acid compound has the following structural formula, where n is 2. Based on the anticancer activity of rotenone, this invention utilizes reduction, esterification, and two amide reactions to successfully link rotenol, dequinoline chloride, and hyaluronic acid via amide bonds. Subsequently, these components are successfully self-assembled into spherical nanoparticles using ultrasound, constructing a rotenol-dequinoline chloride-hyaluronic acid nanosystem for tumor therapy. After targeted delivery of these nanoparticles to tumor cells, both rotenol and dequinoline chloride synergistically act on the mitochondria, forming a dual-drug delivery system that generates a large amount of ROS, thereby inducing apoptosis and effectively inhibiting tumor growth and migration.

The invention relates to a compound composition containing metarhizium anisopliae and rotenone and application of the compound composition, and belongs to the technical field of biopesticides. The effective components of the compound composition are rotenone and metarhizium anisopliae MaGX19S02, and the compounding mass ratio of the metarhizium anisopliae MaGX19S02 to the rotenone is (0.1-9): (0.1-1). According to the invention, by compounding the active components of metarhizium anisopliae and rotenone, the defects that a single component rotenone is high in price, and metarhizium anisopliae is poor in quick-acting property, unstable in pesticide effect and the like are effectively overcome; the compound composition containing the metarhizium anisopliae and the rotenone is an environment-friendly pesticide composition, and the active ingredients of the metarhizium anisopliae and the rotenone are in a reasonable compounding proportion range and have a synergistic effect on prevention and control of tetranychus cinnabarinus.

The invention belongs to the technical field of pesticides, and particularly relates to a botanical pesticide composition and application thereof. Active components of the bactericidal composition comprise rotenone and rhodinol, and the weight ratio of rotenone to rhodinol is (1: 20)-(10: 1). The insecticide disclosed by the invention belongs to a botanical insecticide and is environment-friendly, and the active ingredients have an obvious synergistic effect, so that the dosage of the insecticide can be reduced, and the insecticide resistance of pests can be delayed.

The invention discloses a method for establishing a bee Parkinson's disease model based on characterization of dyskinesia and the like, which is characterized by comprising the following steps of: animal selection: selecting a mature bee as an object for establishing the bee Parkinson's disease model; preparing a modeling diet: preparing a sucrose solution containing casein hydrolysate and rotenone; and model establishment: feeding the mature bees with a modeling diet for 7 days to establish the bee Parkinson's disease model. The invention further discloses an application of the parkinson's disease model, established by the method, of the bacteria bees and / or the sterile bees. The method is easy and convenient to operate and low in cost, the motion distance and the motion speed of the built model bee are remarkably decreased, obvious dyskinesia is shown, and the survival rate of 5 days in an existing bee Parkinson model building technology is effectively increased to about 80% of 7 days from 5 days lower than 30%. The bee Parkinson's disease model established by the method can be used for screening new methods or new drugs for preventing or treating dyskinesia related diseases.

The present invention relates to a composition and the like for preventing, ameliorating or treating mitochondrial complex 1 abnormality diseases, the composition comprising a hydranth (Hydra NDi1-carrying recombinant adeno-associated virus vector. The inventors have confirmed that scAAV-hydranth NDi1 according to the present invention has an effect of restoring reduced respiration of cells caused by rotenone and an effect of inhibiting apoptosis of LHON patient-derived cells caused by rotenone, and thus it is expected to be used in the prevention, amelioration or treatment of LHON and diseases caused by mitochondrial complex 1 abnormalities.

The invention relates to the technical field of biopesticides, in particular to a biopesticide for preventing and treating lepidoptera noctuidae pests and a preparation method of the biopesticide. The pesticide is prepared from the following components in parts by weight: 10 to 20 parts of bacillus thuringiensis subsp. Israelensis powder, 8 to 15 parts of sophocarpidine-rotenone compound liquid, 5 to 12 parts of metarhizium anisopliae spore powder, 3 to 8 parts of trehalose-chitosan grafted copolymer, 4 to 9 parts of nano silicon dioxide loaded peppermint oil, 2 to 6 parts of toosendanin, 0.5 to 2 parts of abamectin, 1 to 4 parts of sodium lignin sulfonate, 2 to 5 parts of xanthan gum and 3 to 7 parts of humic acid. The composition is prepared from the following components in parts by weight: 1-3 parts of vitamin E, 0.3-1 part of vitamin E, 1-3 parts of limonene, 1-3 parts of N-acetyl-5-methoxytryptamine and 2-5 parts of a poly gamma-glutamic acid-chitin compound. The biological pesticide is excellent in control effect, good in stability and persistence, ecological, safe, free of residues, low in application cost, capable of increasing the crop yield, capable of having both economic and ecological benefits and wide in popularization prospect.

The invention relates to a method for preparing a Parkinson mouse model by using a dose incremental method and an application of the Parkinson mouse model. The method comprises the following specific steps: carrying out drug administration intervention on a non-human mammal by adopting a rotenone solution to obtain a Parkinson mouse model, and evaluating the Parkinson mouse model by carrying out behavioral detection and brain tissue detection on the Parkinson mouse model, the administration dosage is 0.9 mg / kg and lasts for 3 days, and then the concentration is gradually increased to 1.8 mg / kg from 0.15 mg / kg and lasts for 6 days; and gradually increasing the dosage to the final dosage of 2.05 mg / kg again, and maintaining for 6 days. Compared with the prior art, the method has the advantages that rotenone is injected into the abdominal cavity of the mouse by using a dose incremental method, the safety of a subject is preferentially guaranteed and certain tolerance is established from the dose far lower than an expected effective dose, and animals are prevented from death due to acute poisoning, so that the Parkinson mouse model is successfully constructed.

The application discloses a Parkinson's disease cell model with mitochondrial damage, a construction method and application thereof. The construction method utilizes a target virus containing a CRISPR / Cas9 system to infect dopaminergic neuron cells, can precisely knock out a gene fragment coding mitochondrial transcription factor A in a nucleus gene of the dopaminergic neuron cells, makes the dopaminergic neuron cells reduce expression of TFAM protein, causes mitochondrial dysfunction, and further forms the Parkinson's disease cell model with mitochondrial damage. The mitochondrial damage feature of the Parkinson's disease cell model provided by the application is hereditary, therefore, after CRISPR / Cas9 gene editing is performed on primary cells, the edited primary cells are cultured to obtain a sufficient number of cell models for subsequent experiments and research. Compared with a method of exposing to rotenone, the method does not need to re-model each time the Parkinson's disease cell model is used, and the subcultured cells can be directly taken.

The invention relates to the technical field of gene therapy, in particular to a method for knocking out a Bax gene on the basis of an HEK293 cell line by utilizing a CRISPR / Cas9 technology, constructing a stable cell line expressed by foreign protein and improving the AAV packaging efficiency. The invention discloses a Bax2.6 cell strain. A pro-apoptosis factor Bax gene is knocked out from an HEK293T cell. Compared with a wild type HEK293T cell, the Bax2.6 cell strain provided by the invention shows higher cell activity under various stress conditions, the cell survival rate of the Bax2.6 cell strain is improved by at least 20% under the treatment conditions of small molecule inhibitors ZV-FMK, Nec-1, puromycin (Puro), cis-platinum (CDDP) and rotenone, and the Bax2.6 cell strain shows excellent environmental tolerance and production robustness. When the Bax2.6 cell strain provided by the invention is used for transient transfection and packaging of an adeno-associated virus (AAV) vector, the copy number of the obtained AAV virus can be up to 2 * 10 copies / mL, and the AAV packaging efficiency is improved by more than 10 times compared with that of a conventional HEK293T cell line.

This invention belongs to the field of medicinal chemistry, specifically relating to a mitochondrial-malonic acid-dequinoline chloride compound, its preparation method, and its application. The rotenol-malonic acid-dequinoline chloride compound has the following structural formula. This invention uses malonic acid as a bridging molecule, covalently binding the rotenone derivative—rotenol—with the mitochondrial-targeting molecule—dequinoline chloride to obtain an anticancer prodrug molecule—rotenol-malonic acid-dequinoline chloride. This prodrug can promote drug accumulation in the mitochondria of cancer cells. Given that dequinoline chloride has a significant apoptosis-promoting function, the synergistic effect of rotenol and dequinoline chloride induces the production of a large amount of reactive oxygen species within cancer cells, thereby exhibiting high anticancer activity and achieving highly effective and low-toxicity anticancer treatment.

The invention discloses a diluent for sorting pig Y sperms as well as a preparation method and application of the diluent. The diluent is prepared from the following components: 40 g / L of glucose, 0.5 g / L of sodium citrate, 0.1 g / L of creatine monohydrate, 0.75 g / L of potassium chloride, 0.0015 g / L of rotenone, 2.38 g / L of HEPES, 0.2 g / L of magnesium chloride and 1.0 g / L of bovine serum albumin. The diluent can amplify the difference of linear movement speed between X and Y sperms of pigs by regulating ATP supply, and can separate the Y sperms with higher movement speed in combination with an upstream sorting method, and the separation efficiency reaches 70-80%. The diluent disclosed by the invention has the characteristics of simplicity, rapidness, high efficiency and low cost in Y sperm separation, is almost harmless to sperms, and is suitable for being applied to boar production.

A method of assessing mitochondria bioenergetics includes obtaining a frozen tissue or frozen biofluid sample of an individual and measuring mitochondria bioenergetics of the frozen tissue or frozen biofluid sample using a substrate for the frozen tissue or biofluid sample. The substrate may include at least one of Glucose; Alamethicin, Nicotinamide adenine dinucleotide (H), and Cytochrome C; Succinate and Rotenone; or Ascorbate+TMPD (Tetramethyl phenylenediamine).

The invention discloses an anti-aging drug screening method based on mitochondrial multilevel analysis-targeted intervention, and particularly relates to the technical field of biological drug manufacturing. The method comprises the following steps: constructing a rotenone-induced aging human mesenchymal stem cell model; the ATP level fluctuation, the active oxygen level, the mitochondrial network fusion and division dynamics and the autophagy flux are monitored in real time by using a fluorescent probe; calculating a baseline function entropy through multi-dimensional data analysis; evaluating the influence of the candidate drugs on the dynamic response entropy of the senescent cells under the condition of mild oxidative stress; an optimal drug is screened and combined with spermidine or urolithin A in a low dosage, and the synergistic effect of the drug in the aspects of maintaining low functional entropy, promoting removal of damaged mitochondria, not causing excessive autophagy and the like is verified. According to the method, multi-dimensional and complete-cycle mitochondrial function evaluation is realized through functional entropy quantification and dynamic stress recovery testing, the method has the advantages of high screening efficiency, objective result and good safety, and a new strategy is provided for the development of anti-aging drugs.