Method for promoting growth and carbon sequestration of scenedesmus by using polyacrylamide in oilfield wastewater

By acclimating Scenedesmus to grow in oilfield wastewater and secreting urease to degrade polyacrylamide, the problem of the difficulty in recycling polyacrylamide in oilfield wastewater treatment was solved, achieving the dual effect of efficient carbon fixation and waste removal.

CN120988845APending Publication Date: 2025-11-21ZHEJIANG UNIV
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Patent Information

Application Number
CN202510883384.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-29
Publication Date
2025-11-21

AI Technical Summary

Technical Problem

In existing technologies, polyacrylamide in oilfield wastewater is difficult to recycle effectively, leading to environmental pollution. Furthermore, using fungi to degrade polyacrylamide is costly and has limited benefits.

Method used

By acclimating Scenedesmus in a gradient manner, it is made to grow in oilfield wastewater containing polyacrylamide and secrete urease, which promotes the degradation of polyacrylamide into polyacrylic acid and ammonia. The urease degradation products are used to enhance amino acid synthesis and energy supply, thus achieving the dual effects of carbon fixation and waste removal.

Benefits of technology

This method achieves efficient degradation of polyacrylamide while fixing carbon, improving the carbon fixation efficiency of microalgae, reducing treatment costs, and providing comprehensive benefits.

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Abstract

The invention relates to a biomass energy utilization technology, and aims to provide a method for promoting scenedesmus growth and carbon sequestration by using polyacrylamide in oilfield wastewater. Comprising the following steps: inoculating an original scenedesmus strain into a photobioreactor filled with a liquid culture medium, providing a proper illumination condition, and introducing CO2 / N2 mixed gas into the reactor; the concentration of polyacrylamide in the liquid culture medium is increased in a gradient increasing mode, and inoculation is repeated multiple times till domestication is completed; scenedesmus strains capable of stably inheriting are selected for enlarged culture, oilfield wastewater containing polyacrylamide is used for preparing a liquid culture medium, and decomposition of polyacrylamide is promoted while carbon sequestration growth of scenedesmus is achieved. The polyacrylamide in the oilfield wastewater is utilized to promote the growth and carbon sequestration of the scenedesmus, the polyacrylamide can be degraded while the carbon sequestration growth is realized, the double results of carbon sequestration and waste removal are realized, and the comprehensive benefit of the method is far better than that of a fungus waste removal scheme in the traditional technology.
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