29 results about "Phosphorus utilization" patented technology

The invention provides application of a transcription factor OsNAC10 in improvement of rice phosphorus utilization and yield. The application comprises any one of the following applications of a rice OsNAC10 gene, the transcription factor OsNAC10 and a recombinant vector containing the OsNAC10 gene: A, improvement of rice phosphorus utilization; b, preparing a product for improving phosphorus utilization of rice; c, increasing the rice yield; and D, preparing a product for improving the rice yield, wherein the nucleotide sequence of the rice OsNAC10 gene is as shown in SEQ ID NO: 1. The invention provides a new theoretical basis and genetic resources for the cultivation of high-efficiency phosphorus varieties of rice.

The invention discloses a rice SPP1 gene and application of the rice SPP1 gene in regulation and control of phosphorus nutrient absorption and accumulation. The polynucleotide sequence of the SPP1 gene is shown as SEQ ID No: 2, and the amino acid sequence of the protein of the SPP1 gene is shown as SEQ ID No: 3. The invention provides the application of the OsSPP1 gene and related biological materials in realizing efficient enrichment of phosphorus element, and particularly, the phosphorus accumulation level of rice can be effectively improved by inhibiting the expression of OsSPP1, and meanwhile, the growth of the rice is not obviously influenced. By changing the expression level of the OsSPP1, the phosphorus enrichment level of the plant is changed, so that the efficient utilization and regulation of the rice on the phosphorus element are realized.

The invention discloses a fertilizer applicable to wheat and capable of increasing the phosphorus utilization rate and a preparation method of the fertilizer, and aims to solve the problem of low phosphorus utilization rate of wheat. A three-layer composite structure of an inner layer quick-acting phosphorus core (monoammonium phosphate and humic acid chelated zinc and iron), a middle layer microbial slow-release layer (calcium alginate wrapped ammonium polyphosphate and glomus mosseae spores) and an outer layer intelligent response layer (chitosan-carboxymethyl cellulose immobilized phytase) is adopted, and processes of low-temperature drying, ellipse forming and the like are combined for preparation. The fertilizer realizes quick-acting-slow-release-intelligent conversion synergy, the phosphorus utilization rate is increased by 20-25% compared with the traditional phosphorus utilization rate, the microorganism survival rate is greater than or equal to 90%, the enzyme stability is improved, the soil phosphorus loss is reduced, and the application is convenient and efficient.

The invention relates to the technical field of biology, in particular to application of ZmMAPKKK70 protein and a coding gene thereof in regulation and control of corn phosphorus utilization, the ZmMAPKKK70 gene is screened out through whole genome correlation analysis, the ZmMAPKKK70 gene is knocked out from corn through a gene editing technology, and an obtained corn mutant material has the advantages that the corn mutant material can be used for regulating and controlling the phosphorus utilization of the corn in a field with phosphate fertilizer or without phosphate fertilizer; the Pseudomonas aeruginosa has the advantages in a plurality of growth indexes, including stability of leaf development, increase of plant height and stem diameter and improvement of final biomass and yield, and especially shows relatively strong adaptive capacity and growth potential under different phosphate fertilizer supply conditions. The corn ZmMAPKKK70 is verified to negatively regulate plant phosphorus utilization and overground biomass, plants which are more tolerant to a low-phosphorus environment and larger in overground biomass are obtained, gene resources are provided for new variety cultivation, and mutant materials have wide agricultural application potential, are particularly suitable for farmland environments with limited resources and insufficient phosphate fertilizer supply and have broad application prospects. Meanwhile, the obvious high-yield potential is also shown under the condition that the phosphate fertilizer is sufficient.

The invention belongs to the technical field of gene engineering, and particularly relates to a molecular marker fragment related to wheat phosphorus efficient utilization characters and application of the molecular marker fragment. The invention provides a molecular marker fragment related to a wheat phosphorus efficient utilization character. The molecular marker fragment comprises a TaMYB4-7A gene promoter region; the nucleotide sequence of a promoter region of the TaMYB4-7A gene is as shown in SEQ ID NO. 1. The molecular marker fragment can be used for screening wheat varieties with efficient phosphorus utilization, the screening efficiency of wheat with efficient phosphorus utilization is improved, and wheat germplasm breeding is facilitated.

The application provides a MhPHR2-MhSCL29 double-gene co-transformation synergistic regulation module and application, and belongs to the field of microbial genetic engineering.In the application, the phosphate-starvation response factor MhPHR2 is used as an upstream regulation hub, directly binds to a P1BS cis-acting element on a promoter of a downstream GRAS family transcription factor MhSCL29, positively activates the transcription of the MhSCL29, and forms a complete phosphate signal-mycorrhizal symbiosis regulation cascade.The nucleotide sequence of MhPHR2 in the synergistic regulation module is as shown in SEQ ID NO.1, and the nucleotide sequence of MhSCL29 is as shown in SEQ ID NO.2.The application improves the mycorrhizal symbiosis potential and the phosphorus utilization efficiency of a stock from the root.

The invention relates to the technical field of plant genetic engineering, and discloses a soybean GmPHR2 gene and an application thereof in regulating the tolerance of soybeans to low phosphorus stress. The nucleotide sequence of the CDS region of the GmPHR2 gene is as shown in SEQ ID NO. 1. Through cloning and functional analysis of the GmPHR2 gene, it is found that the growth ability and phosphorus utilization efficiency of plants in a low-phosphorus environment are remarkably improved through overexpression of the GmPHR2 gene. In order to further verify the function of the gene GmPHR2, the gene GmPHR2 is subjected to heterologous overexpression in soybean, and the result shows that the tolerance of an overexpression plant under low-phosphorus stress is obviously enhanced, the fresh weight and lateral root length of the plant are obviously increased, and the growth condition is obviously improved. The research provides important gene resources and theoretical basis for low-phosphorus-tolerant breeding of crops, is beneficial to cultivation of new soybean varieties adapting to low-phosphorus soil, and improves the production potential of soybeans in a phosphorus-deficient environment.

The invention discloses a rice OsSLRL2 gene and application of the rice OsSLRL2 gene in improvement of rice phosphorus utilization efficiency, plant type optimization and yield, and relates to the technical field of plant genetic engineering. Researches find that the knockout or function deletion of the OsSLRL2 gene can significantly enhance the adaptability of rice under the low-phosphorus condition, which shows that the root system is developed, the tillering is increased, the grain number per spike is increased and the biomass is increased, so that the phosphorus utilization efficiency is significantly improved, the plant type structure is optimized, and the stable yield and high yield under the low-phosphorus condition are achieved. On the contrary, overexpression of the OsSLRL2 leads to root growth limitation and tillering reduction. By regulating and controlling the expression of the OsSLRL2 gene, the efficient utilization of phosphorus and the collaborative optimization of ideal plant types can be realized under the condition of not increasing the input of phosphate fertilizer, and a new molecular target and a new breeding approach are provided for cultivating novel rice varieties with high phosphorus utilization rate and excellent plant types.

This invention discloses a method for preparing and applying a compound fertilizer of filamentous microalgae and biochar for enhancing phosphorus utilization in saline-alkali land, belonging to the field of agricultural resource utilization. This invention achieves stable colonization and functional enhancement of microalgae in the porous structure of biochar through a three-step synergistic process of acid modification, ultrasonic assistance, and co-cultivation. Organic acids are creatively used to modify biochar, optimizing its pore structure and surface functional groups to improve microalgae adhesion. Then, ultrasonic assistance is used to clean the pores of the biochar, promoting the entry of microalgae cells into the pores. Finally, the modified biochar and filamentous microalgae are co-cultivated under light conditions, allowing the microalgae to actively grow and colonize the porous structure of the biochar, forming an integrated active microalgae-biochar compound fertilizer. It has advantages such as increasing the content of available nitrogen and potassium in the soil and significantly improving the bioavailability of existing phosphorus in the soil. Moreover, it can regulate the soil to a neutral range, constructing a healthy and long-lasting soil micro-ecosystem.

The invention discloses a molecular marker related to efficient utilization of slash phosphorus and application of the molecular marker, and belongs to the technical field of molecular biology. The molecular marker is two missense mutation SNP (Single Nucleotide Polymorphism) sites: SNP-1 (620th site, C-G) and SNP-2 (1897th site, AG-GC) located in a slash pine PeSTOP1 gene coding region. Specific genotypes (a GG type of SNP-1 and a GC / GC type of SNP-2) are obviously related to high phosphorus utilization efficiency and a high-low-phosphorus-resistance comprehensive evaluation value. The invention further provides a primer pair and a kit for detecting the marker and an application method of the primer pair and the kit in auxiliary screening of the slash pine with efficient phosphorus utilization. Through the molecular marker, rapid and accurate genotype identification can be realized in the seedling stage, the defects of long period and low efficiency of traditional phenotype screening are overcome, and a core tool is provided for efficient utilization and genetic improvement of slash pine phosphorus and molecular marker assisted breeding.

The invention discloses an active agent serratia marcescens capable of improving phosphorus utilization capacity of tobacco and application of the active agent serratia marcescens, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, the preservation center number is CGMCC No.36318, the preservation date is October 23, 2025, and the strain is classified and named as the active agent serratia marcescens. The active agent serratia marcescens is an efficient inorganic phosphorus decomposing bacterium, and can significantly increase the effectiveness of insoluble inorganic phosphorus in soil, thereby activating the inherent phosphorus library of the soil to promote the growth of tobacco, and having important practical value for promoting the sustainable development of tobacco agriculture.

The application discloses a rice OsSLRL2 gene and application thereof in improving phosphorus utilization efficiency, plant type optimization and yield, and relates to the technical field of plant genetic engineering. Research finds that knockout or functional loss of the OsSLRL2 gene can significantly enhance the adaptability of rice under low-phosphorus conditions, and is manifested as developed root system, increased tillering, improved grain number per panicle and increased biomass, thereby significantly improving phosphorus utilization efficiency, optimizing plant type structure, and realizing stable yield and high yield under low-phosphorus conditions. On the contrary, overexpression of the OsSLRL2 gene leads to limited root growth and reduced tillering. By regulating the expression of the OsSLRL2 gene, the synergistic optimization of high phosphorus utilization efficiency and ideal plant type can be realized without increasing phosphorus fertilizer input, and a new molecular target and breeding approach is provided for cultivating a new rice variety with high phosphorus utilization rate and excellent plant type.

The invention discloses a Burkholderia pyrrocinia capable of improving phosphorus utilization capacity of tobacco and application of the Burkholderia pyrrocinia, the Burkholderia pyrrocinia is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, the preservation center number is CGMCC No.36320, the preservation date is October 23, 2025, and the Burkholderia pyrrocinia is classified and named as Burkholderia pyrrocinia. The strain Burkholderia pyrrocinia is an efficient inorganic phosphorus decomposing bacterium, can significantly increase the effectiveness of insoluble inorganic phosphorus in soil and promote phosphorus absorption and healthy growth of tobacco, and has a wide application prospect.

The invention discloses a Pseudomonas aeruginosa DT-6 and application thereof, and belongs to the technical field of microorganisms. According to the invention, three phosphate-solubilizing bacteria JX-5, JD-7 and DT-6 are screened out, the three phosphate-solubilizing bacteria are determined to have relatively strong dissolving capacity on organophosphorus lecithin, and the DT-6 strain has the most obvious effect, so that the DT-6 is selected as a test strain and is subjected to biological preservation, and the preservation number is CCTCC NO: M2024310. Furthermore, the metabolic regulation and control mechanism of the DT-6 on the cabernet sauvignon seedlings under the condition that available phosphorus in soil is deficient is deeply researched, an efficient local strain resource is provided for improving the utilization of the available phosphorus in the soil, and a theoretical basis and a practical reference are also provided for promoting green agriculture development and ecological environment protection.

The invention relates to the technical field of microorganism application, in particular to arbuscular mycorrhizal fungi capable of improving the phosphorus utilization efficiency of crops and application of the arbuscular mycorrhizal fungi, and the preservation number of the arbuscular mycorrhizal fungi capable of improving the phosphorus utilization efficiency of the crops is CGMCC NO.41940. The arbuscular mycorrhizal fungus spores are added into arbuscular mycorrhizal fungus dominant species (such as alfalfa) potted plants, propagation is carried out by infecting plant root systems, and a substrate containing a large number of arbuscular mycorrhizal fungus spores, namely the arbuscular mycorrhizal fungus fungicide, is obtained. The fertilizer can improve the spore density of arbuscular mycorrhizal fungi in a crop growth environment, improve the infection rate of arbuscular mycorrhizal fungi of crops, and promote recruitment of phosphate solubilizing bacteria and phosphatase activity of crop root systems, thereby improving the ability of crops to utilize insoluble phosphorus in soil. Meanwhile, the utilization rate of phosphate fertilizer and crop yield are increased, the production cost is reduced, and soil and water environment problems caused by excessive application of phosphate fertilizer are improved.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to plant cold tolerance and phosphorus utilization efficiency and application of the molecular marker. On the basis of a genome version number V4, the molecular marker relates to the 799th site and the 800th site of the downstream of a translation start site of a Zm00001d027700 gene of a corn chromosome 1 and the 52nd site to the 63rd site of the downstream of a translation start site of a Zm00001d033503 gene of the corn chromosome 1. Wherein the polymorphism of the 799 site downstream of the translation start site of the Zm00001d027700 gene is G / A, the polymorphism of the 800 site is C / A, and the polymorphism of the 52 site to the 63 site downstream of the translation start site of the Zm00001d033503 gene is existence / deletion of TTCCTAGGGCAA. The molecular marker provided by the invention can be used for comprehensive detection of plant cold resistance and phosphorus utilization efficiency, and auxiliary identification of plant cold resistance and phosphorus utilization efficiency can be realized by detecting polymorphism of the molecular marker in plants. The molecular marker provided by the invention can significantly improve the plant breeding efficiency, and has great application value and practical significance.

This invention discloses a method for preparing and applying a compound fertilizer of filamentous microalgae and biochar for enhancing phosphorus utilization in saline-alkali land, belonging to the field of agricultural resource utilization. This invention achieves stable colonization and functional enhancement of microalgae in the porous structure of biochar through a three-step synergistic process of acid modification, ultrasonic assistance, and co-cultivation. Organic acids are creatively used to modify biochar, optimizing its pore structure and surface functional groups to improve microalgae adhesion. Then, ultrasonic assistance is used to clean the pores of the biochar, promoting the entry of microalgae cells into the pores. Finally, the modified biochar and filamentous microalgae are co-cultivated under light conditions, allowing the microalgae to actively grow and colonize the porous structure of the biochar, forming an integrated active microalgae-biochar compound fertilizer. It has advantages such as increasing the content of available nitrogen and potassium in the soil and significantly improving the bioavailability of existing phosphorus in the soil. Moreover, it can regulate the soil to a neutral range, constructing a healthy and long-lasting soil micro-ecosystem.

The invention provides a biological organic fertilizer containing a compound strain as well as a preparation method and application of the biological organic fertilizer, and belongs to the technical field of biological organic fertilizers. The bacillus velezensis and the bacillus amyloliquefaciens are added, the bacillus velezensis can tolerate certain salinity and alkalinity, the soil fertility can be improved through nitrogen fixation, the utilization rate of phosphorus can be improved by dissolving inorganic phosphorus, the tolerance of plants to environmental stress is enhanced through iron-producing carriers, the growth of seedlings is promoted, and the growth and development of the plants are promoted; the bacillus amyloliquefaciens has the functions of resisting salt and alkali to a certain degree, dissolving phosphorus, dissolving potassium and the like. The bio-organic fertilizer disclosed by the invention is beneficial to remarkably increasing the contents of chlorophyll, proline and soluble sugar of the pakchoi and also reducing the content of malondialdehyde of the pakchoi, so that the oxidative damage of plants is reduced, the stress resistance and quality are improved, the contents of nitrogen, phosphorus and potassium in soil are integrally improved, and the acidification of the soil is remarkably relieved.

The invention relates to the technical field of Amomum villosum planting, in particular to a culture medium for Amomum villosum planting and a preparation method thereof.The culture medium is prepared from 35-45 parts of coco coir, 15-25 parts of perlite, 8-12 parts of vermiculite, 13-17 parts of organic fertilizer, 8-12 parts of loaded biochar, 2-6 parts of novel seaweed extract, 20-25 parts of deionized water and 1-2 parts of organic fungicide. The problems that a traditional substrate is prone to hardening and root rotting are solved, and a comfortable space is provided for root system development; secondly, through adsorption and nitrogen fixation and phosphate solubilization functions of the loaded biochar, the nitrogen and phosphorus utilization rate is increased, and weight losing and efficiency increasing are achieved; meanwhile, the water retention and slow release characteristics of the modified trehalose relieve drought stress, the novel seaweed extract promotes root system development and plant robustness, root rot fungi are inhibited, and pesticide use is greatly reduced; and finally, beneficial microbial communities constructed by the organic fungicide compete for nutrition and secrete antibacterial substances to improve stress resistance, and a green and sustainable rhizosphere environment is integrally created.

The invention discloses a serratia saumannii strain capable of improving phosphorus utilization capacity of tobacco and application of the serratia saumannii strain, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, the preservation center number is CGMCC No.36319, the preservation date is October 23, 2025, and the strain is classified and named as serratia saumannii. The strain serratia salumannii is an efficient inorganic phosphorus decomposing bacterium, inorganic phosphorus which is fixed in soil and cannot be utilized by plants can be effectively activated and converted into soluble available phosphorus, and the process directly improves the effectiveness of phosphorus in the soil, so that the phosphorus absorption efficiency of tobacco is remarkably enhanced, the growth and development of the tobacco are promoted, and the yield of the tobacco is increased. Wide application prospects are realized.

The invention discloses a method for preparing a phosphorus-rich organic fertilizer by using phosphorus-solubilizing bacteria and phosphorus-accumulating bacteria in cooperation with phosphogypsum compost, and belongs to the technical field of organic fertilizers. The method comprises the following steps: taking sawdust and livestock and poultry manure, and uniformly mixing to obtain a mixed material; adding ardealite into the mixed material, uniformly mixing to obtain a compost material, adding the compost material into a closed reactor, adjusting the moisture content, then inoculating phosphate solubilizing bacteria for aerobic composting, performing ventilation aeration and manual turning operation in the composting process, and inoculating phosphate-accumulating bacteria in the composting cooling period to finally obtain the phosphorus-rich organic fertilizer. The phosphogypsum is added into the livestock and poultry manure for phosphorus enrichment treatment, meanwhile, the phosphate solubilizing bacteria are inoculated in the earlier stage of composting, and the phosphate solubilizing bacteria can convert insoluble phosphorus into rapidly available phosphorus which can be absorbed by plants by secreting organic acid, phosphatase and other substances, so that the fertilizer efficiency of the composting is improved; then, phosphorus-accumulating bacteria are inoculated in a composting cooling period, so that the phosphorus content of microbial biomass is improved, the fixation of phosphorus in soil is reduced, and the utilization efficiency of the phosphorus is further improved.

The application discloses a preparation method of a three-acid chelating compound fertilizer, and belongs to the technical field of new-type fertilizers. The three acids include gamma-aminobutyric acid (GABA), polyglutamic acid (PGA) and fulvic acid. The application innovatively adopts a three-step process of "pre-chelation reaction-molecular embedding-low-temperature granulation", first forms pre-chelation complexes of the three acids under specific pH and temperature conditions, then protects the heat-sensitive three acids by a sodium alginate-calcium chloride embedding technology, and finally granulates under a temperature lower than 55 DEG C, thereby solving the technical problem that bioactive substances are prone to inactivation in traditional high-temperature processes. Through the intermolecular synergistic chelation and hierarchical release mechanism of the three acids, the application realizes the synergy of rapid stress resistance response and long-acting nutrient activation, and the phosphorus utilization rate is increased by more than 40%, and is suitable for use in the seedling stage, flowering stage and stress period of crops.

The invention provides a gene BnaPT8 and application thereof, the nucleotide sequence of the gene BnaPT8 is shown as SEQ ID NO: 1, the gene is positioned on a cell membrane, has a transmembrane phosphorus transport effect, and can improve the utilization of rape phosphorus. In soil with normal available phosphorus content, the BnaPT8 gene knockout type rape mutant is cultivated to increase the grain yield. The invention provides a new theoretical basis and genetic resources for cultivation of rape varieties.

The application discloses a kind of detection methods of inositol hexakisphosphate and its application in plant cultivation, the application utilizes the electrostatic and chelation of nano iron oxide and inositol hexakisphosphate Induce nanoparticle aggregation, cause color change or UV-Vis absorption peak shift (200~220 nm), for the rapid detection of inositol hexakisphosphate concentration in soil or nutrient solution, detection limit 0.1 mg / L, total time <10 minutes.The method includes sample extraction, reaction and optical determination steps, suitable for on-site monitoring of phosphorus fixation and fertilizer quality control.The detection results of the application can guide the application of complex, improve the phosphorus utilization efficiency by 20~30%, and enhance the plant cultivation effect (such as alfalfa yield increase by 18%, rice growth period is shortened by about 7 days).The method of the application is suitable for soil phosphorus fixation monitoring.

The invention discloses a method for evaluating the phosphorus utilization efficiency of oat water, which comprises the following steps: selecting healthy and full seeds with uniform size, disinfecting the seeds with sodium hypochlorite for 15 minutes, dividing the disinfected seeds into a group A, a group B, a group C and a group D, planting the group A in soil with sufficient water and sufficient phosphorus, planting the group B in soil with sufficient water but lack of phosphorus, planting the group C in soil with sufficient water but lack of phosphorus, and planting the group D in soil with sufficient water but lack of phosphorus; the D group is planted in water-deficient and phosphorus-deficient soil, the irrigation amount of the four groups of treatment is recorded, and harvesting is conducted in the full ripening stage; the method comprises the following steps: drying harvested oat grains to constant weight, measuring the dry biomass weight of the grains, then crushing a sample, measuring the phosphorus concentration of the grains, and calculating the phosphorus absorption amount; calculating the evaluation index phosphorus utilization efficiency and water utilization efficiency according to the grain biomass, the phosphorus absorption amount and the irrigation amount; evaluating the water phosphorus utilization efficiency of the oat variety. The method provided by the invention can quickly and effectively realize the phosphorus utilization efficiency evaluation of the oat water.

The invention discloses a rice phosphorus utilization related gene OsPHL as well as an encoded protein and application thereof, and the gene OsPHL provided by the invention is a DNA (Deoxyribose Nucleic Acid) molecule as shown in 1), 2), 3) or 4): 1) a DNA molecule as shown in SEQ ID NO.1; 2) a DNA molecule as shown in SEQ ID NO. 2; 3) a DNA molecule which is hybridized with the DNA sequence limited by 1) or 2) under strict conditions and is used for coding the protein; and 4) a DNA molecule which has more than 90% of homology with the DNA sequence limited by 1) or 2) or 3) and is used for coding the plant phosphorus utilization related protein. The invention also provides a protein coded by the gene, the protein affects the utilization of plant phosphorus, and the protein and the coding gene thereof can be applied to plant genetic improvement.

The invention belongs to the technical field of aquaculture, and particularly relates to a method for improving phosphorus utilization efficiency of lateolabrax japonicus and improving lipid metabolism of lateolabrax japonicus in a low-phosphorus state. The fish collagen peptide is added into the low-phosphorus feed, so that the abnormal lipid metabolism of the lateolabrax japonicus fed by the low-phosphorus feed is remarkably improved, the growth performance of the lateolabrax japonicus is improved, the content of phosphorus in the feed is reduced, and the pollution of phosphorus loss to the environment is reduced. Results of embodiments show that the low-phosphorus feed added with the fish collagen peptide can significantly reduce liver fat deposition of lateolabrax japonicus, improve body indexes such as liver-to-body ratio and viscera-to-body ratio, and restore the content of total triglyceride in serum to a normal level. Supplementing the fish collagen peptide is an effective method for relieving negative reactions generated when the lateolabrax japonicus is fed with the low-phosphorus feed.

The application relates to a composite biological agent for improving phosphorus utilization efficiency of dairy cows, a preparation method and application in the technical field of phosphorus emission reduction of dairy cows. The composite biological agent comprises butyric acid carboxy bacteria, DNA and phytase connected with nano zinc oxide; the butyric acid carboxy bacteria, the DNA and the phytase connected with nano zinc oxide are sequentially connected. The butyric acid carboxy bacteria in the composite biological agent of the application has strong colonization ability in the intestinal tract, so that the retention time of the phytase connected with the butyric acid carboxy bacteria in the intestinal tract is improved, the role of hydrolyzing phytic acid is effectively played, the nano zinc oxide connected with the phytase can protect the activity of the phytase, the phytase continuously converts phytic acid in feed, the composite biological agent has a long application effect time on the dairy cows, the phosphorus emission reduction effect is good, the components in the composite biological agent are biologically safe, so the composite biological agent can be well applied to reducing phosphorus emission in the process of dairy cow breeding and has a good application prospect.

The invention discloses application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding, the acid phosphatase OsPAP23 is found to have organophosphorus hydrolase activity and participate in rice phosphorus absorption and utilization regulation for the first time, expression of the acid phosphatase OsPAP23 gene is increased in a plant body, the acid phosphatase activity of rice is improved, and the yield of rice phosphorus is increased. Further, the utilization efficiency of phosphorus in the body and the utilization efficiency of organic phosphorus are improved. According to the invention, research on phosphorus absorption and transport regulation and control mechanisms is enriched, a reference mechanism is provided for corresponding phosphorus deficiency stress of rice, and the created PAP23 overexpression strain is expected to show growth and yield advantages in fields with organic fertilizers.