43 results about "Phosphorus utilization" patented technology

The invention relates to the technical field of fertilizers, in particular to a seaweed-containing synergistic monoammonium phosphate fertilizer as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing a seaweed extract with beta-cyclodextrin, performing microencapsulation, and performing spray drying to obtain an embedded material; mixing with monoammonium phosphate and polyglutamic acid, and granulating; spraying salt-resistant compound bacteria liquid on the surfaces of the particles; and finally, coating the particles with a chitosan-sodium alginate composite film. According to the prepared synergistic monoammonium phosphate fertilizer finished product, the phosphorus utilization rate of monoammonium phosphate saline-alkali soil can be increased, the phosphorus fixation rate can be greatly reduced, the retention rate of active ingredients of the seaweed extract is greatly increased compared with that in the prior art, the rhizosphere survival rate of salt-tolerant flora is greatly increased, and the salt-tolerant flora, the seaweed extract and monoammonium phosphate cooperate to promote crop yield increase.

The invention discloses a soybean-corn intercropping nitrogen and phosphorus synergistic efficient utilization method based on rhizosphere signals, and relates to the technical field of microbial technology and agricultural engineering, a BX-pH dual sensor array is deployed in a root system intersection area, and the concentration and microscale pH change of benzoxazoline are monitored in real time; processing data by using two-stage feature transformation and a time sequence attention bidirectional GRU model, calculating a nitrogen-phosphorus disconnection coefficient and positioning risk coordinates; then, a buffer-antioxidant bifunctional nano-carrier is injected along the risk coordinates, neutralization acidification is carried out, and active oxygen is eliminated; after acidification and active oxygen signals are reduced to threshold values, DualFlow functional bacteria are inoculated at the same coordinate, and nutrition flow is recovered through phosphorus dissolution and nitrogen fixation synergistically; finally, a transport gene activation signal and nitrogen fixation intensity are continuously monitored through a BX-pH sensor array, and root zone water and proton outflow is adjusted in combination with variable-frequency pulse irrigation. Nitrogen and phosphorus utilization efficiency is remarkably improved, chemical fertilizer dependence is reduced, and crop yield and soil health are improved.

The invention provides application of a transcription factor OsNAC10 in improvement of rice phosphorus utilization and yield. The application comprises any one of the following applications of a rice OsNAC10 gene, the transcription factor OsNAC10 and a recombinant vector containing the OsNAC10 gene: A, improvement of rice phosphorus utilization; b, preparing a product for improving phosphorus utilization of rice; c, increasing the rice yield; and D, preparing a product for improving the rice yield, wherein the nucleotide sequence of the rice OsNAC10 gene is as shown in SEQ ID NO: 1. The invention provides a new theoretical basis and genetic resources for the cultivation of high-efficiency phosphorus varieties of rice.

The invention discloses a rice SPP1 gene and application of the rice SPP1 gene in regulation and control of phosphorus nutrient absorption and accumulation. The polynucleotide sequence of the SPP1 gene is shown as SEQ ID No: 2, and the amino acid sequence of the protein of the SPP1 gene is shown as SEQ ID No: 3. The invention provides the application of the OsSPP1 gene and related biological materials in realizing efficient enrichment of phosphorus element, and particularly, the phosphorus accumulation level of rice can be effectively improved by inhibiting the expression of OsSPP1, and meanwhile, the growth of the rice is not obviously influenced. By changing the expression level of the OsSPP1, the phosphorus enrichment level of the plant is changed, so that the efficient utilization and regulation of the rice on the phosphorus element are realized.

The application discloses a preparation method of a catalytic cracking octane value additive, and first forms a Y-type molecular sieve filter cake layer, then loads ZSM-5 molecular sieve exchanged by containing phosphorus wastewater on the Y-type molecular sieve filter cake, recovers the excess phosphorus in the filtrate through the Y-type molecular sieve filter cake layer, reduces the loss of phosphorus, and the excess phosphorus can also modify the Y-type molecular sieve, and simultaneously improves the thermal stability and hydrothermal stability of the Y-type molecular sieve. The added humic acid is a multi-element organic complex, has the function of fixing phosphorus elements, makes the phosphorus elements not easy to flow away with the filtrate, and further improves the phosphorus utilization rate. The preparation method of the catalytic cracking additive provided by the application recycles the phosphorus-containing wastewater of the additive microspheres for the molecular sieve ion exchange process, can effectively reduce the water consumption and the consumption of the phosphorus-containing exchange liquid in the additive production process, and is favorable for reducing the production cost of the additive.

The invention relates to a wheat low-phosphorus response transcription factor TaERF112 and application thereof, and belongs to the technical field of plant bioengineering. The low-phosphorus response transcription factor TaERF112 gene is cloned from wheat, the CDS region sequence of the gene is 882bp, 294 amino acids are encoded, the molecular weight is 31 and 572.92 Da, and the gene is hydrophilic protein. By analyzing the TaERF112 gene structure, protein physicochemical properties, subcellular localization and species evolution characteristics, the characteristics and function prediction of the gene are known. Furthermore, a wheat taerf112 mutant is created by using a CRISPR / Cas9 technology, phenotypic characters, biomass and phosphorus utilization characteristics of a transformed strain under different phosphorus conditions are evaluated, and the important effect of the wheat taerf112 mutant in regulation and control of a wheat root system and phosphorus homeostasis is preliminarily revealed. Theoretical basis and gene resources are provided for efficient genetic improvement of wheat phosphorus.

The application provides a preparation method of a catalytic cracking propylene aid, a Y-type molecular sieve filter cake layer is first formed, then ZSM-5 molecular sieve exchanged by containing phosphorus wastewater is loaded on the Y-type molecular sieve filter cake, surplus phosphorus in the filtrate is recovered by the Y-type molecular sieve filter cake layer, the loss of phosphorus is reduced, meanwhile, the surplus phosphorus can also modify the Y-type molecular sieve, and the thermal stability and the hydrothermal stability of the Y-type molecular sieve are simultaneously improved. The added humic acid is a multi-element organic complex, has the function of fixing phosphorus elements, makes the phosphorus elements not easy to flow away with the filtrate, and further improves the phosphorus utilization rate. The preparation method of the catalytic cracking aid provided by the application recycles the phosphorus-containing wastewater of the aid microspheres for the molecular sieve ion exchange process, can effectively reduce the water consumption and the consumption of the phosphorus-containing exchange liquid in the aid production process, and is favorable for reducing the production cost of the aid.

The invention discloses a fertilizer applicable to wheat and capable of increasing the phosphorus utilization rate and a preparation method of the fertilizer, and aims to solve the problem of low phosphorus utilization rate of wheat. A three-layer composite structure of an inner layer quick-acting phosphorus core (monoammonium phosphate and humic acid chelated zinc and iron), a middle layer microbial slow-release layer (calcium alginate wrapped ammonium polyphosphate and glomus mosseae spores) and an outer layer intelligent response layer (chitosan-carboxymethyl cellulose immobilized phytase) is adopted, and processes of low-temperature drying, ellipse forming and the like are combined for preparation. The fertilizer realizes quick-acting-slow-release-intelligent conversion synergy, the phosphorus utilization rate is increased by 20-25% compared with the traditional phosphorus utilization rate, the microorganism survival rate is greater than or equal to 90%, the enzyme stability is improved, the soil phosphorus loss is reduced, and the application is convenient and efficient.

The invention discloses a sphingobium sp. W6 strain for promoting plants to adapt to a low-phosphorus environment and application of the sphingobium sp. W6 strain, and relates to the technical field of microorganisms, the preservation number of the sphingobium sp. W6 is GDMCCNO: 66821, and the sphingobium sp. W6 is preserved in the Guangdong Microbial Culture Collection Center on August 7th, 2025, and the preservation address is the 5th floor, No. 59 building, No. 100 Courtyard, Xianlie Middle Road, Guangzhou. The invention also provides application of the sphingolipid bacteria W6 in improving the plant phosphorus absorption efficiency and preparing a product for improving the plant phosphorus absorption efficiency. The sphingolipid strain W6 has high IAA yield and can significantly promote plant root growth, so that the phosphorus utilization efficiency of plants in a low-phosphorus environment is improved, and the sphingolipid strain W6 has a great promotion effect on plant growth.

The invention discloses a detection method of inositol hexaphosphate and application thereof in plant cultivation, the electrostatic and chelation effects of nano iron oxide and inositol hexaphosphate are utilized to induce aggregation of nano particles to cause color change or UV-Vis absorption peak shift (200-220 nm), the detection method is used for rapidly detecting the concentration of inositol hexaphosphate in soil or a nutrient solution, the detection limit is 0.1 mg / L, the detection time is short, and the detection result is accurate. Total time lt; the time is 10 minutes. The method comprises the steps of sample extraction, reaction and optical determination, and is suitable for field monitoring of phosphorus fixation and fertilizer quality control. The detection result can guide the application of the compound, the phosphorus utilization efficiency is improved by 20-30%, and the plant cultivation effect is enhanced (for example, the alfalfa yield is increased by 18%, and the growth period of rice is shortened by about 7 days). The method provided by the invention is suitable for soil phosphorus fixation monitoring.

The invention relates to the technical field of biology, in particular to application of ZmMAPKKK70 protein and a coding gene thereof in regulation and control of corn phosphorus utilization, the ZmMAPKKK70 gene is screened out through whole genome correlation analysis, the ZmMAPKKK70 gene is knocked out from corn through a gene editing technology, and an obtained corn mutant material has the advantages that the corn mutant material can be used for regulating and controlling the phosphorus utilization of the corn in a field with phosphate fertilizer or without phosphate fertilizer; the Pseudomonas aeruginosa has the advantages in a plurality of growth indexes, including stability of leaf development, increase of plant height and stem diameter and improvement of final biomass and yield, and especially shows relatively strong adaptive capacity and growth potential under different phosphate fertilizer supply conditions. The corn ZmMAPKKK70 is verified to negatively regulate plant phosphorus utilization and overground biomass, plants which are more tolerant to a low-phosphorus environment and larger in overground biomass are obtained, gene resources are provided for new variety cultivation, and mutant materials have wide agricultural application potential, are particularly suitable for farmland environments with limited resources and insufficient phosphate fertilizer supply and have broad application prospects. Meanwhile, the obvious high-yield potential is also shown under the condition that the phosphate fertilizer is sufficient.

The invention belongs to the technical field of gene engineering, and particularly relates to a molecular marker fragment related to wheat phosphorus efficient utilization characters and application of the molecular marker fragment. The invention provides a molecular marker fragment related to a wheat phosphorus efficient utilization character. The molecular marker fragment comprises a TaMYB4-7A gene promoter region; the nucleotide sequence of a promoter region of the TaMYB4-7A gene is as shown in SEQ ID NO. 1. The molecular marker fragment can be used for screening wheat varieties with efficient phosphorus utilization, the screening efficiency of wheat with efficient phosphorus utilization is improved, and wheat germplasm breeding is facilitated.

The application provides a MhPHR2-MhSCL29 double-gene co-transformation synergistic regulation module and application, and belongs to the field of microbial genetic engineering.In the application, the phosphate-starvation response factor MhPHR2 is used as an upstream regulation hub, directly binds to a P1BS cis-acting element on a promoter of a downstream GRAS family transcription factor MhSCL29, positively activates the transcription of the MhSCL29, and forms a complete phosphate signal-mycorrhizal symbiosis regulation cascade.The nucleotide sequence of MhPHR2 in the synergistic regulation module is as shown in SEQ ID NO.1, and the nucleotide sequence of MhSCL29 is as shown in SEQ ID NO.2.The application improves the mycorrhizal symbiosis potential and the phosphorus utilization efficiency of a stock from the root.

The invention relates to the technical field of plant genetic engineering, and discloses a soybean GmPHR2 gene and an application thereof in regulating the tolerance of soybeans to low phosphorus stress. The nucleotide sequence of the CDS region of the GmPHR2 gene is as shown in SEQ ID NO. 1. Through cloning and functional analysis of the GmPHR2 gene, it is found that the growth ability and phosphorus utilization efficiency of plants in a low-phosphorus environment are remarkably improved through overexpression of the GmPHR2 gene. In order to further verify the function of the gene GmPHR2, the gene GmPHR2 is subjected to heterologous overexpression in soybean, and the result shows that the tolerance of an overexpression plant under low-phosphorus stress is obviously enhanced, the fresh weight and lateral root length of the plant are obviously increased, and the growth condition is obviously improved. The research provides important gene resources and theoretical basis for low-phosphorus-tolerant breeding of crops, is beneficial to cultivation of new soybean varieties adapting to low-phosphorus soil, and improves the production potential of soybeans in a phosphorus-deficient environment.

The invention discloses a rice OsSLRL2 gene and application of the rice OsSLRL2 gene in improvement of rice phosphorus utilization efficiency, plant type optimization and yield, and relates to the technical field of plant genetic engineering. Researches find that the knockout or function deletion of the OsSLRL2 gene can significantly enhance the adaptability of rice under the low-phosphorus condition, which shows that the root system is developed, the tillering is increased, the grain number per spike is increased and the biomass is increased, so that the phosphorus utilization efficiency is significantly improved, the plant type structure is optimized, and the stable yield and high yield under the low-phosphorus condition are achieved. On the contrary, overexpression of the OsSLRL2 leads to root growth limitation and tillering reduction. By regulating and controlling the expression of the OsSLRL2 gene, the efficient utilization of phosphorus and the collaborative optimization of ideal plant types can be realized under the condition of not increasing the input of phosphate fertilizer, and a new molecular target and a new breeding approach are provided for cultivating novel rice varieties with high phosphorus utilization rate and excellent plant types.

The invention belongs to the technical field of gene regulation and control, and particularly relates to application of a Zm5PTase25 gene in regulation and control of corn low-phosphorus tolerance. The sequence of the Zm5PTase25 gene is shown as SEQ ID NO.1. The silent Zm5PTase25 gene reduces the resistance of corn to low phosphorus stress, and the over-expressed Zm5PTase25 gene improves the resistance of corn to low phosphorus stress; the improvement of the resistance of corn to low-phosphorus stress shows that under the low-phosphorus stress, the absorption and utilization of plants to phosphorus are enhanced, the rhizosphere acidification capability is weakened, and the growth and development are obviously enhanced. Therefore, the Zm5PTase25 gene has important theoretical and practical significance for creating a corn material with efficient phosphorus utilization and low phosphorus tolerance.

This invention discloses a method for preparing and applying a compound fertilizer of filamentous microalgae and biochar for enhancing phosphorus utilization in saline-alkali land, belonging to the field of agricultural resource utilization. This invention achieves stable colonization and functional enhancement of microalgae in the porous structure of biochar through a three-step synergistic process of acid modification, ultrasonic assistance, and co-cultivation. Organic acids are creatively used to modify biochar, optimizing its pore structure and surface functional groups to improve microalgae adhesion. Then, ultrasonic assistance is used to clean the pores of the biochar, promoting the entry of microalgae cells into the pores. Finally, the modified biochar and filamentous microalgae are co-cultivated under light conditions, allowing the microalgae to actively grow and colonize the porous structure of the biochar, forming an integrated active microalgae-biochar compound fertilizer. It has advantages such as increasing the content of available nitrogen and potassium in the soil and significantly improving the bioavailability of existing phosphorus in the soil. Moreover, it can regulate the soil to a neutral range, constructing a healthy and long-lasting soil micro-ecosystem.

The invention discloses a molecular marker related to efficient utilization of slash phosphorus and application of the molecular marker, and belongs to the technical field of molecular biology. The molecular marker is two missense mutation SNP (Single Nucleotide Polymorphism) sites: SNP-1 (620th site, C-G) and SNP-2 (1897th site, AG-GC) located in a slash pine PeSTOP1 gene coding region. Specific genotypes (a GG type of SNP-1 and a GC / GC type of SNP-2) are obviously related to high phosphorus utilization efficiency and a high-low-phosphorus-resistance comprehensive evaluation value. The invention further provides a primer pair and a kit for detecting the marker and an application method of the primer pair and the kit in auxiliary screening of the slash pine with efficient phosphorus utilization. Through the molecular marker, rapid and accurate genotype identification can be realized in the seedling stage, the defects of long period and low efficiency of traditional phenotype screening are overcome, and a core tool is provided for efficient utilization and genetic improvement of slash pine phosphorus and molecular marker assisted breeding.

The invention discloses an active agent serratia marcescens capable of improving phosphorus utilization capacity of tobacco and application of the active agent serratia marcescens, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, the preservation center number is CGMCC No.36318, the preservation date is October 23, 2025, and the strain is classified and named as the active agent serratia marcescens. The active agent serratia marcescens is an efficient inorganic phosphorus decomposing bacterium, and can significantly increase the effectiveness of insoluble inorganic phosphorus in soil, thereby activating the inherent phosphorus library of the soil to promote the growth of tobacco, and having important practical value for promoting the sustainable development of tobacco agriculture.

The invention discloses a rice OsHAD5 mutant and application thereof, and relates to the technical field of biotechnology and plant genetic engineering, and the technical scheme is characterized in that the rice acid phosphatase OsHAD5 gene is subjected to directed mutation through a gene editing technology, and the rice acid phosphatase OsHAD5 mutant is obtained by performing different levels of phosphorus treatment and phosphorus content determination on a mutant plant. It is found that the phosphorus content in rice leaves can be remarkably increased after the gene is mutated, and the key effect of the gene in rice phosphorus metabolism is revealed. The invention has important application value in rice breeding and agricultural production, can effectively improve the phosphorus utilization efficiency of rice and reduce the use of phosphate fertilizer, and has wide application prospect.

The application discloses a rice OsSLRL2 gene and application thereof in improving phosphorus utilization efficiency, plant type optimization and yield, and relates to the technical field of plant genetic engineering. Research finds that knockout or functional loss of the OsSLRL2 gene can significantly enhance the adaptability of rice under low-phosphorus conditions, and is manifested as developed root system, increased tillering, improved grain number per panicle and increased biomass, thereby significantly improving phosphorus utilization efficiency, optimizing plant type structure, and realizing stable yield and high yield under low-phosphorus conditions. On the contrary, overexpression of the OsSLRL2 gene leads to limited root growth and reduced tillering. By regulating the expression of the OsSLRL2 gene, the synergistic optimization of high phosphorus utilization efficiency and ideal plant type can be realized without increasing phosphorus fertilizer input, and a new molecular target and breeding approach is provided for cultivating a new rice variety with high phosphorus utilization rate and excellent plant type.

The invention discloses a Burkholderia pyrrocinia capable of improving phosphorus utilization capacity of tobacco and application of the Burkholderia pyrrocinia, the Burkholderia pyrrocinia is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation address is Beijing, the preservation center number is CGMCC No.36320, the preservation date is October 23, 2025, and the Burkholderia pyrrocinia is classified and named as Burkholderia pyrrocinia. The strain Burkholderia pyrrocinia is an efficient inorganic phosphorus decomposing bacterium, can significantly increase the effectiveness of insoluble inorganic phosphorus in soil and promote phosphorus absorption and healthy growth of tobacco, and has a wide application prospect.

The invention discloses a Pseudomonas aeruginosa DT-6 and application thereof, and belongs to the technical field of microorganisms. According to the invention, three phosphate-solubilizing bacteria JX-5, JD-7 and DT-6 are screened out, the three phosphate-solubilizing bacteria are determined to have relatively strong dissolving capacity on organophosphorus lecithin, and the DT-6 strain has the most obvious effect, so that the DT-6 is selected as a test strain and is subjected to biological preservation, and the preservation number is CCTCC NO: M2024310. Furthermore, the metabolic regulation and control mechanism of the DT-6 on the cabernet sauvignon seedlings under the condition that available phosphorus in soil is deficient is deeply researched, an efficient local strain resource is provided for improving the utilization of the available phosphorus in the soil, and a theoretical basis and a practical reference are also provided for promoting green agriculture development and ecological environment protection.

The application of a rice gene in regulating phosphorus homeostasis, the nucleotide sequence of the rice OsSPL17 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the rice OsSPL17 gene is shown as SEQ ID NO. 2. By cloning the rice gene OsSPL17, a transgenic vector is constructed, and OsSPL17 gene overexpression rice and OsSPL17 gene deletion rice are obtained. The phenotypic traits of the transgenic rice are determined, and it is found that compared with the wild type rice, the phosphorus content of the transgenic rice changes, the overexpression rice has a lower phosphorus absorption rate in a high phosphorus environment, and the degree of high phosphorus toxicity of the plant is reduced; and the phosphorus absorption rate of the deletion rice increases in a low phosphorus environment, and the phosphorus utilization rate of the rice is improved. It is shown that the OsSPL17 gene plays an important regulatory function in the phosphorus utilization of the rice, maintains the phosphorus homeostasis of the rice, and is helpful to improve the phosphorus fertilizer utilization rate.

The invention relates to the technical field of microorganism application, in particular to arbuscular mycorrhizal fungi capable of improving the phosphorus utilization efficiency of crops and application of the arbuscular mycorrhizal fungi, and the preservation number of the arbuscular mycorrhizal fungi capable of improving the phosphorus utilization efficiency of the crops is CGMCC NO.41940. The arbuscular mycorrhizal fungus spores are added into arbuscular mycorrhizal fungus dominant species (such as alfalfa) potted plants, propagation is carried out by infecting plant root systems, and a substrate containing a large number of arbuscular mycorrhizal fungus spores, namely the arbuscular mycorrhizal fungus fungicide, is obtained. The fertilizer can improve the spore density of arbuscular mycorrhizal fungi in a crop growth environment, improve the infection rate of arbuscular mycorrhizal fungi of crops, and promote recruitment of phosphate solubilizing bacteria and phosphatase activity of crop root systems, thereby improving the ability of crops to utilize insoluble phosphorus in soil. Meanwhile, the utilization rate of phosphate fertilizer and crop yield are increased, the production cost is reduced, and soil and water environment problems caused by excessive application of phosphate fertilizer are improved.

The invention relates to the technical field of plant breeding, in particular to a molecular marker related to plant cold tolerance and phosphorus utilization efficiency and application of the molecular marker. On the basis of a genome version number V4, the molecular marker relates to the 799th site and the 800th site of the downstream of a translation start site of a Zm00001d027700 gene of a corn chromosome 1 and the 52nd site to the 63rd site of the downstream of a translation start site of a Zm00001d033503 gene of the corn chromosome 1. Wherein the polymorphism of the 799 site downstream of the translation start site of the Zm00001d027700 gene is G / A, the polymorphism of the 800 site is C / A, and the polymorphism of the 52 site to the 63 site downstream of the translation start site of the Zm00001d033503 gene is existence / deletion of TTCCTAGGGCAA. The molecular marker provided by the invention can be used for comprehensive detection of plant cold resistance and phosphorus utilization efficiency, and auxiliary identification of plant cold resistance and phosphorus utilization efficiency can be realized by detecting polymorphism of the molecular marker in plants. The molecular marker provided by the invention can significantly improve the plant breeding efficiency, and has great application value and practical significance.

This invention discloses a method for preparing and applying a compound fertilizer of filamentous microalgae and biochar for enhancing phosphorus utilization in saline-alkali land, belonging to the field of agricultural resource utilization. This invention achieves stable colonization and functional enhancement of microalgae in the porous structure of biochar through a three-step synergistic process of acid modification, ultrasonic assistance, and co-cultivation. Organic acids are creatively used to modify biochar, optimizing its pore structure and surface functional groups to improve microalgae adhesion. Then, ultrasonic assistance is used to clean the pores of the biochar, promoting the entry of microalgae cells into the pores. Finally, the modified biochar and filamentous microalgae are co-cultivated under light conditions, allowing the microalgae to actively grow and colonize the porous structure of the biochar, forming an integrated active microalgae-biochar compound fertilizer. It has advantages such as increasing the content of available nitrogen and potassium in the soil and significantly improving the bioavailability of existing phosphorus in the soil. Moreover, it can regulate the soil to a neutral range, constructing a healthy and long-lasting soil micro-ecosystem.

The invention provides a biological organic fertilizer containing a compound strain as well as a preparation method and application of the biological organic fertilizer, and belongs to the technical field of biological organic fertilizers. The bacillus velezensis and the bacillus amyloliquefaciens are added, the bacillus velezensis can tolerate certain salinity and alkalinity, the soil fertility can be improved through nitrogen fixation, the utilization rate of phosphorus can be improved by dissolving inorganic phosphorus, the tolerance of plants to environmental stress is enhanced through iron-producing carriers, the growth of seedlings is promoted, and the growth and development of the plants are promoted; the bacillus amyloliquefaciens has the functions of resisting salt and alkali to a certain degree, dissolving phosphorus, dissolving potassium and the like. The bio-organic fertilizer disclosed by the invention is beneficial to remarkably increasing the contents of chlorophyll, proline and soluble sugar of the pakchoi and also reducing the content of malondialdehyde of the pakchoi, so that the oxidative damage of plants is reduced, the stress resistance and quality are improved, the contents of nitrogen, phosphorus and potassium in soil are integrally improved, and the acidification of the soil is remarkably relieved.

The present invention relates to a method for improving nitrogen and phosphorus utilization efficiency in red soil and its application. The method comprises applying microalgae cell sap to the red soil; the microalgae include Scenedesmus and / or Chlorella. The present invention develops a novel method and strategy for improving nitrogen and phosphorus utilization efficiency in red soil. This method and strategy is simple to implement and is suitable for cultivating various crops in red soil. It also significantly improves nitrogen and phosphorus utilization efficiency in red soil and crop yields.

The invention discloses application of a GmCXIP4 gene in regulation and control of plant phosphorus nutrition and growth promotion. Research shows that the GmCXIP4 gene participates in regulation of soybean root growth and phosphorus nutrition absorption, and through construction and identification of overexpression and knockout carrier materials, the overexpression GmCXIP4 gene can inhibit plant growth and reduce absorption and accumulation of phosphorus nutrition by plants; after the GmCXIP4 gene is knocked out, plant growth can be remarkably promoted, absorption and accumulation of phosphorus nutrition by the plant are promoted, and the phosphorus content of the plant is increased; under low phosphorus stress, plant growth and phosphorus nutrition absorption can be promoted. As a new gene resource for regulating plant growth and phosphorus nutrition, the GmCXIP4 gene plays an important role in improving plant phosphorus nutrition and promoting plant growth, provides an important gene resource for cultivating low-phosphorus-resistant plants, and plays an important role in adapting to low-phosphorus-condition growth of plants and improving phosphorus utilization efficiency.