33 results about "Leaf development" patented technology

The invention discloses application of a tobacco NtPDX2 gene in tobacco plant height and stem leaf development. The tobacco plant height, stem thickness and leaf area are adjusted by regulating and controlling tobacco NtPDX2 gene expression. The CDS sequence of the tobacco NtPDX2 gene is as shown in SEQ ID NO. 1; the amino acid sequence of the protein encoded by the gene is as shown in SEQ ID No.2. The NtPDX2 gene is edited by using a CRISPR / Cas9 mediated gene editing technology to obtain an editing material with reduced plant height, thinned stem and reduced leaf area, and the NtPDX2 gene is over-expressed by using a transgenic technology to obtain an over-expression material with increased plant height, thickened stem and enlarged leaf area. The gene serves as a positive regulation factor to participate in growth and development of the plant height, the stalk and the leaves, and a genetic material and a theoretical basis are provided for oriented improvement of a new tobacco variety for regulating the plant height and the stalk thickness.

The invention discloses application of an MsMYB-ZD gene in regulating and controlling the leaf size and yield of medicago sativa, and belongs to the technical field of plant genetic engineering. According to the invention, an MsMYB-ZD transcription factor for regulating and controlling the leaf size of medicago sativa is cloned by using transcriptome analysis; experimental data show that the MsMYB-ZD gene presents a gradually up-regulated expression mode in a leaf development process; compared with wild alfalfa Gongnong No.1 1-16 single plants, the leaf area of the overexpressed MsMYB-ZD transgenic single plant is averagely enlarged by 77%, and the expression quantity and the leaf area of the transgenic line show a remarkable positive correlation relationship; the fresh grass yield of a transgenic single plant is increased by 158.7%, and the hay yield of a single plant is increased by 207.5%. The results show that the gene has important significance for improving the yield of medicago sativa.

The invention belongs to the field of plant genetic engineering, and specifically relates to the application of an agent for overexpressing the MsGRF3 gene in promoting plant leaf development and a method for promoting plant leaf development. The invention discloses the MsGRF3 gene and its application, wherein the nucleotide sequence of the MsGRF3 gene is shown in SEQ ID NO.3; and discloses the application of the gene, a plant overexpression vector and a plant chimeric overexpression vector in regulating leaf size and leaf development in alfalfa.

The invention belongs to the field of genetic engineering, and relates to a mung bean VrLAL1 (LANCEOLATE LEAVES1) protein, a mung bean VrLAL1 gene and application of the mung bean VrLAL1 protein and the mung bean VrLAL1 gene in regulation and control of leaf size and pod length, M5311 wild species and al1 mutant species are hybridized, and the VrLAL1 gene is finely positioned on the basis of an existing mung bean molecular marker. The method comprises the following steps: selecting a plurality of candidate genes in a candidate interval, simultaneously performing transcriptome sequencing analysis on a mutant, screening and identifying a plurality of candidate genes in the candidate interval according to a transcriptome result, finding a mutant gene VrLAL1 gene, designing a primer, and performing PCR (Polymerase Chain Reaction) first cloning to obtain the VrLAL1 gene. The VrLAL1 gene has an obvious effect on regulation and control of organ size, leaf development and pod development, and can be used for variety improvement of legume crops.

The utility model provides a planting and cultivating device, and relates to the technical field of agriculture. The planting and cultivating device comprises a cabinet body used for installing the device, an adjusting mechanism used for adjusting the internal space of the device is arranged in the cabinet body, a moving mechanism is arranged in the cabinet body, the adjusting mechanism comprises sliding blocks and sliding rods, the sliding blocks are connected to the two sides of the inner wall of the cabinet body in a sliding mode, and the sliding rods are arranged on the sliding blocks. The sliding rod is connected to the surface of the inner wall of the sliding block in a sliding mode and penetrates through the sliding block. According to the planting and cultivating device, the adjusting mechanism is arranged, so that the problems that the internal space of an existing planting and cultivating device is difficult to adjust, after plants grow to a certain height, the growth space of the plants is easily limited, the plants are short and small, leaf development is poor, even growth malformation possibly occurs, and cultivation work is not facilitated are solved; the beneficial effects that the internal space of the device can be adjusted to enable the plants to have sufficient growth space, plant growth is facilitated, and cultivation work is facilitated are achieved.

The invention discloses a white clover leaf development regulation gene TrKNOX9 and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO.1. The gene encodes a protein containing 383 amino acids, has a KNOX family typical structural domain, and is positioned in a cell nucleus and a cell membrane. By constructing an overexpression vector PHB-35S-TrKNOX9 and transforming plants, the number of leaflets can be remarkably increased, and the photosynthetic efficiency and yield can be improved. Experiments show that the quantity of rosette leaves of an arabidopsis thaliana strain with overexpression of TrKNOX9 is remarkably increased, and the form of the leaves is optimized; yeast function verification proves that the strain promotes growth. The specific primer pair and the transformation method provided by the invention are suitable for cultivating high-yield and stress-resistant pasture varieties and shortening the breeding period. The gene provides an efficient technical means for germplasm improvement of white clover and similar plants.

This invention provides a method for selecting plants that possess desired traits regarding the internode length of the main stem. [Solution] One aspect of the present invention is a selection method that includes a step of selecting plants using the length of the young shoot at the third leaf development stage as an indicator.

The invention provides a top grafting method for promoting loquats to bloom and bear fruits in advance. The inventor takes' Huabai No.1 'or' Huabai No.2 'as scions, takes Huayu seedless No.1 as a stump-head tree species, takes a branch at the middle lower part of a spring shoot which does not vertically grow on the periphery of a crown of a specific adult tree fruiting in successive years and has the diameter of more than or equal to 1 cm as a scion, takes a loquat tree flowering and fruiting in successive years as a stump-head tree, and cuts back and grafts from the position with the height of 0.4-0.6 m of the loquat tree plant; the top-grafted scions grow and develop quickly and can bloom and bear fruits in the current year, and the yield can be achieved in the second year through the cooperation of water-soluble compound fertilizer after germination and leaf development in the flowering stage and MS microelement fertilizer sprayed in the specific time. Compared with a traditional top grafting method, the method is 1-2 years earlier.

The invention discloses a PyYABBY gene of a pear and application of the PyYABBY gene. The PyYABBY gene separated from Dangshan pear and capable of promoting pear leaf enlargement has a nucleotide sequence as shown in SEQ ID No.1, and an amino acid sequence coded by the PyYABBY gene is as shown in SEQ ID No.2 in a sequence table. In order to verify the function of the PyYABBY gene, a yabby mutant arabidopsis thaliana (lacking the YABBY gene) is adopted, and a supplementation experiment is carried out. The results show that the leaf of the PyYABBY back-complemented strain is obviously larger than that of the yabby mutant (without the YABBY gene) and that of the wild-type arabidopsis thaliana, and further confirms that the PyYABBY gene plays a positive regulation role in leaf size development and can promote leaf enlargement. A new gene resource is provided for molecular mechanism research of pear tree leaf development, and meanwhile, a potential application target is provided for pear tree variety improvement and molecular breeding.

The invention discloses medicago truncatula leaf development protein as well as a coding gene and application thereof. The method comprises the following steps: firstly, cloning an MtPIF4 gene related to plant leaf development from wild medicago truncatula R108 through sequence analysis, then overexpressing the MtPIF4 gene in medicago truncatula through a transgenic technology, and identifying and screening a transgenic MtPIF4 medicago truncatula strain with stable inheritance. And finally, through phenotype analysis on the transfected MtPIF4 medicago truncatula strain, it is found that compared with a wild type R108, the transfected MtPIF4 medicago truncatula strain has obvious phenotypes of leaf enlargement, main stem thickening, branch number increase and plant height increase. The invention lays a theoretical foundation for researching the MtPIF4 gene in regulating and controlling leaf development in plants.

Leaf development, especially in leafy greens, is crucial for crop productivity. In various plants, DA1, a pivotal ubiquitin receptor that negatively regulates leaf growth, and EOD1 (ENHANCER1 OF DA1), an E3 ubiquitin ligases that interacts with DA1 through its RING domain, influence organ size. In this disclosure, in lettuce (Lactuca sativa), homologs genes were identified for both DA1 and EOD1, and da1 single mutants, da1 / eod1 double mutants, and DA1 overexpressing lines (DA1ox) were created. The da1 / eod1 double mutant exhibited a striking phenotype with larger organ size, accelerated growth, and increased biomass, resulting in higher yields and rapid growth traits pivotal for Controlled Environment Agriculture (CEA). Additionally, the da1 / eod1 double mutant displayed a pronounced ‘stay-green’ phenotype, characterized by elevated chlorophyll b levels, which implies extended shelf life, and enhanced postharvest quality, both traits indispensable for leafy vegetables in CEA settings. In contrast, the DA1ox line exhibited early senescence and diminished organ size. Based on these findings that spotlight the nuanced roles of the DA1-EOD1 module in modulating plant growth phases and its potential to revolutionize breeding strategies for improved yield and postharvest longevity in CEA-cultivated leafy greens, this disclosure provides guide RNAs for gene editing of DA1 and EOD1, and transformed lettuce having improved organ size and postharvest quality.

The invention relates to the technical field of biology, in particular to application of ZmMAPKKK70 protein and a coding gene thereof in regulation and control of corn phosphorus utilization, the ZmMAPKKK70 gene is screened out through whole genome correlation analysis, the ZmMAPKKK70 gene is knocked out from corn through a gene editing technology, and an obtained corn mutant material has the advantages that the corn mutant material can be used for regulating and controlling the phosphorus utilization of the corn in a field with phosphate fertilizer or without phosphate fertilizer; the Pseudomonas aeruginosa has the advantages in a plurality of growth indexes, including stability of leaf development, increase of plant height and stem diameter and improvement of final biomass and yield, and especially shows relatively strong adaptive capacity and growth potential under different phosphate fertilizer supply conditions. The corn ZmMAPKKK70 is verified to negatively regulate plant phosphorus utilization and overground biomass, plants which are more tolerant to a low-phosphorus environment and larger in overground biomass are obtained, gene resources are provided for new variety cultivation, and mutant materials have wide agricultural application potential, are particularly suitable for farmland environments with limited resources and insufficient phosphate fertilizer supply and have broad application prospects. Meanwhile, the obvious high-yield potential is also shown under the condition that the phosphate fertilizer is sufficient.

The present invention discloses a Medicago truncatula leaf development protein, its coding gene and applications. First, through sequence analysis, the present invention clones the gene related to plant leaf development from wild-type Medicago truncatula R108, MtPIF4 then overexpresses the gene in Medicago truncatula through transgenic technology, and MtPIF4 identifies and screens out the stably inherited transgenic MtPIF4 Medicago truncatula lines. Finally, through phenotypic analysis of the transgenic MtPIF4 Medicago truncatula lines, it is found that compared with the wild-type R108, the transgenic MtPIF4 Medicago truncatula lines show obvious phenotypes of larger leaves, thicker main stems, increased number of branches and increased plant height. The present invention lays a theoretical foundation for studying the MtPIF4 gene regulating leaf development in plants.

The invention discloses an Anemonium aureum SkORC3 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the SkORC3 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the SkORC3 gene is as shown in SEQ ID NO. 2. According to the invention, the gene is constructed into an overexpression vector and is converted into arabidopsis thaliana, so that the key function of the SkORC3 in regulating and controlling the morphogenesis of plant leaves is disclosed for the first time. A transgenic arabidopsis thaliana plant over-expressing the gene shows remarkable rosette leaf dysplasia, and the specific phenotypes comprise double main vein bifurcated heart-shaped leaves, symmetrical twin leaves at the top end of a petiole, uniaxial special-shaped leaves and the like. The invention provides a new gene resource and tool for molecular mechanism research of plant leaf development, and has potential application value in the field of ornamental plant leaf type molecular breeding.

The invention provides an application of an FveBRI1 gene in regulation and control of strawberry inflorescence branching, a coding sequence of the FveBRI1 gene is shown as SEQ ID NO.1, and an amino acid sequence of protein coded by the FveBRI1 gene is shown as SEQ ID NO.2. The invention further provides an application of the FveBRI1 gene in regulation and control of strawberry inflorescence branching. The research finds that the wild type inflorescence of the forest strawberry belongs to a top branching type, and the inflorescence is changed into a middle branching type and a base branching type after the FveBRI1 gene is mutated; in addition, FveBRI1 mutation also affects leaf development and leads to leaf rounding and middle small petiole elongation, the leaf mutation phenotypes enable mutants to be recognized in the seedling stage, and convenience is provided for early screening. From the aspect of application, the strawberry inflorescence structure can be optimized by regulating and controlling the expression of the FveBRI1, so that the yield and the quality of fruits are improved, and the FveBRI1 has a relatively great application prospect.

The present invention discloses a sweet potato root tuber and leaf development-related protein IbbZIP11 and its encoding gene and application, belonging to the field of biotechnology. The present invention discloses an IbbZIP11 protein or a substance that regulates the expression of the IbbZIP11 protein encoding gene or a substance that regulates the activity or content of the IbbZIP11 protein in regulating plant leaf and / or root development and / or plant yield and in plant breeding; the IbbZIP11 protein is a protein whose amino acid sequence is SEQ ID No.1. The present invention can improve the photosynthetic rate of sweet potatoes, enlarge the root tubers, and increase the yield by knocking out the IbbZIP11 gene through gene editing technology or silencing the IbbZIP11 gene after transcription by dsRNA interference, and is a gene with great application value to the sweet potato industry.

The invention belongs to the technical field of crop cultivation, and particularly relates to a method for improving the salt tolerance of asparagus seedlings. Growth of asparagus seedlings under salt stress is remarkably inhibited, and the growth shows that stem and leaf development is slow, root growth is blocked, and photosynthetic capacity is reduced. The invention provides a method for improving the salt tolerance of asparagus seedlings, and experiments show that a composite nutrient solution rich in a silicon source and a selenium source is externally applied to the asparagus seedlings in the two-leaf one-core stage, the dosage of the composite nutrient solution applied each time is 1.1 cubic meters per mu to 1.4 cubic meters per mu, the composite nutrient solution is applied once every 2-3 days, and continuous treatment is performed for 14-20 times, so that the salt tolerance of the asparagus seedlings is improved. Growth indexes such as fresh weight, dry weight, root length and stem diameter of the asparagus seedlings and photosynthetic parameters such as net photosynthetic rate and chlorophyll content can be recovered to be close to normal levels, and damage of salt stress to the asparagus seedlings can be effectively relieved. The method is easy and convenient to operate and low in cost, and a feasible nutrition regulation and control strategy is provided for efficient cultivation of the asparagus in the salinized soil.

The invention discloses an application of a BjuPR10 gene in regulation and control of plant leaf development and flowering time, a nucleotide sequence of the BjuPR10 gene is shown as SEQ ID NO.1, and an amino acid sequence coded by the BjuPR10 gene is shown as SEQ ID NO.2. The invention further discloses an application of the BjuPR10 gene in regulation and control of plant leaf development and flowering time. The overexpression technology finds that BjuPR10 gene up-regulation expression promotes brassicaceous plants such as stem nodule mustard to bolt and flower in advance and enables leaves to generate more leaf cracks, and the BjuPR10 gene can be applied to delaying the flowering process and has a good application prospect in genetic engineering breeding. A theoretical basis and gene resources are provided for genetic breeding of quality and yield of tumorous stem mustard and other plants, a new choice is provided for genetic improvement of the plants, and meanwhile, the biological function of the PR10 protein is perfected.

The invention belongs to the field of plant genetic engineering, and discloses application of a CaFtsH8 metalloproteinase gene of pepper in leaf development in the seedling stage of pepper. The full-length CDS sequence of the capsicum CaFtsH8 gene is as shown in SEQ ID NO: 1, and a high-specificity segment selected from gene silencing VIGS is as shown in SEQ ID NO: 2. After the CaFtsH8 gene is silenced in a pepper inbred line material Z14, a silenced single plant has an obvious euphylla whitening phenotype, and chloroplast development and photosynthesis are obviously blocked, which indicates that the gene is very important for normal development of pepper in a seedling stage, so that the gene can be applied to molecular design breeding of pepper and has a wide application prospect. Normal growth and development of the pepper in the seedling stage are guaranteed through genotype selection.

The present invention discloses a tobacco leaf development regulatory gene Ntfb1 and its application, belonging to the technical field of plant genetic engineering, wherein the genome sequence of the Ntfb1 gene is shown in SEQ ID NO.1, the CDS sequence is shown in SEQ ID NO.3, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. The present invention finds that tobacco plants overexpressing the Ntfb1 gene grow slowly, have abnormal leaf development, and are shorter in height, while tobacco leaves significantly increase in size and the number of tobacco leaves increases after gene editing, indicating that the Ntfb1 gene participates in leaf growth and development as a negative regulatory factor.

The invention discloses application of casein kinase in regulation and control of flowering and leaf development of medicago sativa, and belongs to the technical field of biological genetic engineering. The multifunctionality of MsPPK3 as a key regulatory factor in coordination of vegetative growth and reproductive growth balance of medicago sativa is clarified for the first time, and specifically, MsPPK3 positively regulates plant reproductive growth and negatively regulates plant vegetative growth. Wherein the positive regulation and control of the reproductive growth of the plant is embodied in overexpression early flowering and silence late flowering. The negative regulation and control of plant vegetative growth is characterized in that the overexpressed plant is in a fine stem scattered form, and leaves are miniaturized, thinned and / or weakened form; the silent plants present a thick stem cluster type, a large leaf size, a thick leaf and / or a strengthened leaf. The method has great significance in the aspects of cultivation of different new varieties / new strains of alfalfa and the like.

The invention discloses a citrus shoot length and leaf color state detection method and system based on colorimetric plate correction, and relates to the field of crop monitoring, and the method comprises the steps: fixing a colorimetric plate below a citrus shoot, and synchronously collecting image information containing a complete shoot region and a colorimetric plate region by using a mobile phone; on the basis of grid lines on the colorimetric plate, determining the branch length and the growth stage of the citrus; performing color calibration based on a standard color block on the colorimetric plate, generating a corrected image, and constructing a training set in combination with the branch length and growth stage information; building a neural network model based on a deep learning technology, and performing model training by using the training set to obtain an evaluation model of citrus branch tip and leaf development; and performing state identification on the corrected image of the to-be-detected branch tip by using the evaluation model of the citrus branch tip and leaf development, obtaining the current branch tip length, and judging whether the current leaf color is in a reasonable range or not. According to the invention, the growth condition of the citrus branch tip can be clearly and accurately known, and the identification speed and accuracy are improved.

This invention belongs to the field of molecular biology, and particularly relates to the application of the GhLEC1-1A gene and its encoded protein in cotton leaf development. The amino acid sequence of the encoded protein is shown in SEQ ID NO.2. This invention successfully introduced the GhLEC1-1A overexpression vector into plants and screened out positive overexpression plants. Compared with wild-type plants, the leaf growth and development morphology of the positive overexpression plants differed from that of the wild type, exhibiting increased leaf area and a final leaf shape resembling a chicken-foot leaf. These morphological changes provide important gene targets and germplasm resources for cotton molecular breeding, and offer technical support for the widespread application of the chicken-foot leaf technique in cotton production.

The application discloses a Medicago polymorpha MYB transcription factor and a coding gene and application, relates to the technical field of biology, the MYB transcription factor gene nucleotide sequence is shown as SEQ ID NO.1; the plant overexpression vector and the RNA interference expression vector of the gene are disclosed; the application of the gene, the plant overexpression vector and the RNA interference expression vector in flavonoid metabolism regulation or / and leaf development in Medicago polymorpha is disclosed.The application clones a MsMYB206 transcription factor gene involved in flavonoid metabolism regulation or palm compound leaf formation of Medicago polymorpha, studies the expression mode of the gene in Medicago polymorpha, compares the total flavone content and leaf shape of wild type and Medicago polymorpha with the MsMYB206 gene, and can be applied to cultivate a variety with more small leaves per leaf, improve the leaf stem ratio of cultivated Medicago polymorpha, thereby improving the yield and nutritional value.

The invention discloses a method for delaying green turning of gold-leaf plants by synergistically applying gibberellin and abscisic acid, and belongs to the technical field of horticultural plant cultivation. According to the method, gibberellin and abscisic acid with effective doses are applied in a foliage spraying mode in the gold leaf period of the early leaf development stage. The technology aims at regulating and controlling an expression network of pigment metabolism related genes by utilizing a synergistic effect of two hormones so as to reduce net accumulation of chlorophyll and promote net accumulation of carotenoid, so that a relatively low chlorophyll / carotenoid ratio is maintained, and finally the green turning of leaves is delayed. Pigment metabolism of leaves of the woody ornamental plant is regulated and controlled through the exogenous plant growth regulating substance, so that the golden yellow ornamental period of the woody ornamental plant is stabilized and prolonged. The method can be applied to ulmus pumila 'JinYe' and other gold-leaf plants with similar green turning phenomena. The method has the advantages that the method can be applied to the ulmus pumila 'JinYe' and other gold-leaf plants with similar green turning phenomena;

The invention provides application of alginate oligosaccharide in relieving atrazine phytotoxicity of tobacco. The alginate oligosaccharide can effectively relieve the phytotoxicity caused by atrazine residues. In the form, the alginate oligosaccharide can obviously improve the growth of inhibited plant height and the development of leaves, and alleviate the malformation symptom caused by phytotoxicity; in the physiological level, the inhibition effect of atrazine on plant photosynthetic metabolism is relieved by activating and repairing a damaged photosynthesis system; on the rhizosphere microorganism level, beneficial microorganism enrichment is promoted, plant growth is promoted, and the phytotoxicity resistance of plants is enhanced.

The invention belongs to the technical field of molecular biology, and particularly relates to a polianthes tuberose PtNPY2 gene and a protein, an expression vector, a biological material and application thereof. Aiming at the problem that research on gene sequence information and functional genes of polianthes tuberosa is blank, leaf and flower organs of polianthes tuberosa are subjected to transcriptome analysis, an NPY2 gene is separated and is named as a PtNPY2 gene through amino acid conserved domain comparison, and a nucleotide sequence of the PtNPY2 gene is shown as SEQ ID NO.1. Experiments find that the expression quantity of the gene is remarkably improved in the organ formation process, then a plant expression vector is constructed and transferred into tobacco for functional analysis, and it is found that the gene can change the tobacco leaf development process, change the leaf shape and thicken leaf veins, so that the leaf shape is peculiar, and the tobacco quality is improved. The gene is a high-value target gene for regulating and controlling organ development of tuberose plants and carrying out genetic improvement. The important reference is provided for deeply researching the molecular mechanism of plant organ development and cultivating new varieties with peculiar leaf shapes.

The application discloses application of BnWUS in regulating cell differentiation and leaf shape development of Brassica napus. The DNA nucleotide sequence of the BnWUS gene is shown as SEQ ID NO. 1, the sequence length is 951 bp, and the encoded amino acid sequence is shown as SEQ ID NO. 2. Functional analysis of Brassica napus genetic transformation shows that BnWUS is involved in regulating cell differentiation and leaf shape development of Brassica napus, overexpression of BnWUS can realize regulation of the leaf shape of Brassica napus, and the yield per plant is improved, thereby providing a theoretical basis for plant type improvement breeding of Brassica napus.

The invention relates to the technical field of plant genetic engineering. The invention provides an MsGWAS1 coding gene, and a nucleotide sequence of the MsGWAS1 coding gene is shown as SEQ ID NO.3. The invention also provides a gene for detecting the MsGWAS1 gene. The invention also provides an application of the MsGWAS1 coding gene in promoting leaf development, and the leaf development shows that the plant leaf area is increased according to the application. According to the application, the leaf development shows that the number of plant leaves is increased. The MsGWAS1 gene participating in alfalfa leaf size regulation is cloned, the function of the MsGWAS1 gene is verified through genetic transformation of arabidopsis thaliana, and the leaf area of transgenic arabidopsis thaliana is increased. Research on the regulation and control mechanism of the leaf size in the leaf development process has important significance on cultivation and selection of excellent alfalfa germplasm resources.

The application provides a compound microbial agent for promoting growth of tobacco plants and application thereof. The compound microbial agent comprises Bacillus altitudinis ZXY01, Bacillus subtilis LGT-2 and Rhodopseudomonas palustris. The compound microbial agent of the application comprises Bacillus altitudinis ZXY01 which can effectively promote release of potassium ions, help to improve the potassium nutrient level of tobacco plants, and provide support for stem robustness and material transportation; Rhodopseudomonas palustris has nitrogen fixation effect, can increase nitrogen source supply, and provide material basis for leaf development; Bacillus subtilis LGT-2 can promote root development, improve nutrient absorption efficiency, synergistically improve growth vigor of tobacco plants, increase environmental tolerance of the whole microbial group, and regulate the growth rate of the other two strains through metabolic products, thereby avoiding the problem of over-proliferation of a single strain due to the absence of Bacillus subtilis, and enabling the three strains to form a stable microbial group in the rhizosphere.