36 results about "Leaf development" patented technology

Belonging to the field of gene technology in molecular biology, the invention relates to a betula platyphylla BplSPL1 gene for promoting precocious flowering and an encoded protein thereof. The invention is characterized in that the betula platyphylla BplSPL1 gene for promoting precocious flowering has a nucleotide sequence shown as SEQ ID NO:1 in a sequence table, and has an amino acid sequence shown as SEQ ID NO:2. The invention provides the betula platyphylla flowering gene BplSPL1 and applies the gene to construction of a BplSPL1 gene's plant expression vector, and the constructed plant expression vector is subjected to agrobacterium impregnation of an arabidopsis thaliana plant so as to obtain a transgenic arabidopsis thaliana plant and achieve precocious flowering. Therefore, the gene has the function of inducing precocious flowering of arabidopsis thaliana and can regulate plant leaf development. The gene provided by the invention utilizes genetic engineering technology to regulate betula platyphylla flowering time and leaf development, provides gene resources and theoretical basis, and has great application value.

The invention relates to a band girdling method for promoting fixed point sprouting of terminalia neotaliala Capuron trunks and belongs to the technical field of tree planting. The method specifically comprises the steps that 1, trunks are cut, wherein plants with soil balls are dug by adopting a tree transplanting method before new leaves of terminalia neotaliala Capuron grow out, and then trunk cutting is performed at the height of 4-6 m of the trunk; 2, band girdling is performed, a first band girdling port is designed at the position, 1.5 m away from a tree head, of each trunk, and band girdling ports are upwards designed from the first band girdling port at intervals of 80 cm; 3, temporary planting is performed, wherein the terminalia neotaliala Capuron undergoing band girdling processing on the same day is transferred into root control bags through a crane and is supported; 4, watering is performed, root fixing water is supplied enough, a rooting agent is irrigated to roots, and soil is kept wet in a later period; 5, fertilization is performed, wherein trees begin to put forth buds under the band girdling ports after 25-35 days after temporary planting, and topdressing can be performed after leaf development and ageing; 6, pests are killed, wherein thiacloprid and fenitrothion missible oil are adopted to kill cerambycidae and anomala corpulenta respectively. By adopting the method, fixed point sprouting of plants is achieved, tree crown layers are better restored, the disease resistance is strong, and the survival rate is high.

The invention relates to a method for quickly constructing a leaf curtain for date trees and a special leaf fertilizer. The method is characterized in that the special fertilizer mainly comprising allantoin, DA-6, hypersensitive protein, BR, urea, water-soluble phosphoric anhydride, K2SO4, HBO3, FeSO4, CuSO4, ZnSO4, MnSO4 and ammonium molybdate is used as a spraying solution to spray the date trees so as to realize quick construction of the leaf curtain for the date trees. Compared with the prior art, the method and the special leaf fertilizer have the advantages that a remixing preparation is adopted, and meanwhile, the special leaf fertilizer contains plant growth substances, inorganic nutrients (major and trace elements) and organic nutrients, are complete in nutrients, convenient to use and stable in effect; by the synergistic effect of multiple substances, the plant growth hormone is supplemented from outside, and the condition that inorganic or organic nutrients are independently used, but the internal growth power is poor is avoided, so that the condition of unobvious effect is avoided, the nutritional condition of development of leaves of the date trees is effectively improved, the quick development of the leaves is enhanced to construct the leaf curtain in advance, and the basic condition for high yield of dates is guaranteed, especially the need for photothermal resources; due to strong sources, the benefit for construction and maintenance of warehouses is achieved; when the sources and the warehouses are developed in a coordination manner, organic coordination of high yield and quality can be obtained.

The invention relates to a zinc finger protein gene OsRLZP for regulation and control of a paddy rice leaf shape and a use thereof. The zinc finger protein gene OsRLZP has a nucleotide sequence shown in the formula of SEQ ID NO: 1 in the sequence table. A protein coded by the zinc finger protein gene OsRLZP has an amino acid sequence shown in the formula of SEQ ID NO: 2 in the sequence table. The gene full-length coding sequence shown in the formula of SEQ ID NO: 1 is inserted into an eukaryotic vector so that an eukaryotic recombinant plasmid excessively expressing the gene OsRLZP is obtained, and the eukaryotic recombinant plasmid is transformed into paddy rice so that an OsRLZP-overexpression transgenic plant is obtained. An experiment proves that the gene shown in the formula of SEQ ID NO: 1 can control paddy rice leaf development. Compared with a wild type plant, the OsRLZP up-regulation transgenic plant leaf has thick midrib, narrow leaf, involute and vertical leaves of the whole plant and less leaf surface hair. Therefore, the zinc finger protein gene OsRLZP has regulation and control effects on paddy rice leaf development and can be used in paddy rice plant-improved genetic breeding.

The invention provides a method for obtaining a multifoliaolate alfalfa material by using an MsPALM1 artificial site-directed mutant. Firstly, a target fragment is selected in an exon region of a alfalfa compound leaf development regulatory gene MsPALM1, a plant CRISPR/Cas9 targeting recombinant vector MsCRISPR/Cas9:PALM1 is constructed, alfalfa cells are introduced and regenerated into seedlings,functional deletion mutation in the MsPALM1 gene of the alfalfa cells is induced by repair after shearing, mutant plants are screened by restriction endonuclease digestion and/or target deep sequencing of the target fragment of a regenerated strain genome to obtain the strain carrying allelic MsPALM1 genes and with the functional deletion mutation, and after phenotypic identification, compound leaves of the regenerated plants are confirmed to be changed from three leaflets to five leaflets. Experiments show that the method can rapidly obtain the multifoliaolate alfalfa material, the breedingcycle is short, and the obtained material is unchanged in other agronomic traits except for the five pinnate compound leaves, is stable in the traits, and can quickly obtain a stable genetically homozygous mutant with four allele mutations.

The invention discloses a method for improving the survival rate of transplanted big osmanthus trees in summer. The method comprises the steps of pre-transplanting treatment, seedling digging and transportation, hole digging and planting, and post-planting maintenance, wherein a carbendazim solution is uniformly sprayed on soil balls of the dug big osmanthus trees after seedling digging, plantingpits are backfilled with backfilling soil during transplanting, the backfilling soil consists of soil, river sand and an organic fertilizer, and the backfilling thickness of the backfilling soil is 0.2-0.3 fold of the depth of a planting pit, and the planted trees are also surrounded by backfilling soil. According to the method, by spraying the carbendazim solution on the soil balls, sterilizationand water supplement are achieved simultaneously to promote the survival of the soil balls; by backfilling with a mixture of soil, river sand and the organic fertilizer, nutrients are slowly supplemented, and roots are promoted to absorb water; one and a half months after planting, a micro element fertilizer consisting of zinc sulfate heptahydrate, ferrous sulfate, magnesium sulfate and borax isapplied to the trees, trace elements are supplemented, and the root growth and branch and leaf development of the plants are promoted. The provided transplanting method is suitable for a current landscaping out-of-season construction normalization form, and the survival rate of the transplanted osmanthus trees in summer is improved.

The invention discloses a planting method of an ornamental tea tree. The planting method comprises the following steps: firstly, reclaiming a garden plot; secondly, improving soil; thirdly, transplanting the tea tree; fourthly, performing water storage improvement on the soil; fifthly, pruning the tea tree; sixthly, performing topdressing; seventhly, managing the tea tree. The planting method disclosed by the invention has the following advantages and the effects: firstly, a pH value of the soil is adjusted to a pH value suitable for growing the tea tree, so that the survival rate of the tea tree in the processes of field planting and transplanting is improved; secondly, an accommodating chamber filled with a water storing agent is formed around of the tea tree, so that the utilization efficiency of water is improved; thirdly, the water storing agent can be used for absorbing the water, and absorbing and releasing fertilizer, so that the utilization efficiency of the fertilizer is improved; by foliar fertilization, nutrients are supplemented, the leaf development is promoted and the ornamentation of the tea tree is improved. Besides, by adoption of a method for artificially catching and killing pests on the tea tree, no pesticide residue on the tea tree is guaranteed, and the effects of saving water and guaranteeing high utilization rate of the fertilizer, high ornamentation, safety and environmental protection are realized.

The invention discloses a floating seedling culturing method for culturing healthy strong seedlings through biological organic liquid fertilizer. The method includes the steps of water adding, fertilizer applying, disc putting and managing, wherein water is drained into a floating seedling culturing pool, and the water depth is 8 cm to 12 cm; then according to the volume ratio of the biological organic liquid fertilizer to water of 1:(20-80), raw liquid of the biological organic liquid fertilizer is added into water; floating discs with sowed seeds are put into the floating seedling culturing pool, and fertilizer applying and managing can be carried out with a conventional floating seedling culturing method. The biological organic liquid fertilizer selected in the floating seedling culturing method has the effects of activating soil nutrients, exciting crop growth, improving the tobacco seedling quality and increasing dry matter accumulation, and is directly added into water with the multi-point applying method, using is convenient, and labor and time are saved; in the applying effect aspect, seedling culturing fertilizer special for tobacco can be completely replaced with the biological organic liquid fertilizer in the seedling stage, and the biological organic liquid fertilizer is suitable for all main cultivated varieties of flue-cured tobacco production, and also has the effects of obviously promoting tobacco seedling growth, leaf development and coordinate growth ground and underground in summer and winter with the low air temperature in the seedling stage.

The invention discloses an application of a microbial fermentation preparation in biological prevention and treatment on Empoasca pirisuga Matumura. According to the invention, bacillus altitudinis, bacillus subtilis and bacillus licheniformis are fermented in a liquid fermentation broth added with betel nuts, oleander leaves and sasangua cakes, a large amount of plant secondary metabolites aimingat Empoasca pirisuga Matumura are generated in the fermentation liquid, so that the Empoasca pirisuga Matumura is favorably killed, meanwhile, surface active substances such as lipopeptide substancesand the like are generated, so that plant resistance is enhanced, non-specific resistance induced by secondary products is generated through induction, a large number of plant growth regulating substances are generated in fermentation liquor, normal physiological activities of plants are facilitated, inorganic nutrients of the plants are increased, and the tree vigor of the plants is enhanced. According to the method, Empoasca pirisuga Matumura can be prevented and treated, tea tree leaf development can be promoted, photosynthesis can be enhanced, leaf quality can be improved, the improvementdegree of tea scale insects after spraying can exceed 80% or above, meanwhile, pesticide residues can be reduced, and autumn tea picking safety can be improved.

The invention relates to a composite herbicide for a peanut field, which belongs to the field of pesticides and comprises the following components in percentage by weight: 1 to 40 percent of lactofen, 1 to 40 percent of clethodim, 2 to 18 percent of emulsifier, and 2 to 96 percent of solvent. The composite herbicide is applied to stem leaves of weeds at the leaf development stage with 2 to 4 leaves in the peanut field through spraying, and can effectively prevent and eliminate grassy weeds and broad leaved weeds; and compared with the single usage of the lactofen and the clethodim, the composite herbicide can enlarge a herbicide controlling spectrum, improve the peanut yield, and reduce the chemical using cost.

The invention discloses a water-soluble fertilizer formula for alleviating seedling stiffness and prompting root growth in a seedling stage. The NPK (nitrogen, phosphorus and potassium) content of pure potassium fulvate is 5%, and the NPK content of pure amino acid raw powder is 18%. A preparation method of the water-soluble fertilizer formula includes the following steps: after planting, mixing 80% of fulvic acid with 20% of amino acid, and irrigating the roots by a 1000-times water solution; in the second and third leaf development stages, mixing 50% of the fulvic acid with 50% of the aminoacid, and irrigating the roots by a 2000-times water solution; in the sixth to tenth leaf development stages, mixing 20% of the fulvic acid with 80% of the amino acid, and irrigating the roots by a 1000-times water solution. The water-soluble fertilizer formula has the advantages that the root growth of grape seedlings can be effectively promoted, and the germination process can be accelerated; dormancy of grape root systems can be interrupted through root irrigation, rapid rooting can be promoted, and bud eye germination and new leaf development can be promoted; the seedling growth is benefited, and the immunity in the seedling stage is improved; capillary roots can grow rapidly, and absorption can be accelerated; NPK, various amino acids and various amino acid nutrients required for theseedlings in the seedling stage are supplemented, and accordingly, the growth of the grape seedlings is accelerated.

The invention belongs to the technical field of gene engineering, and particularly relates to an SNP (Single Nucleotide Polymorphism) site for detecting a watermelon peripheral leaf shape, a closely linked molecular marker and application. The molecular marker provided by the invention can identify the watermelon leaf shape character from the molecular level, can be directly used for molecular marker-assisted breeding of a watermelon peripheral leaf shape material, improves the breeding selection efficiency, and accelerates the breeding process; in addition, a physical map between the two molecular markers can be obtained by utilizing the molecular markers, so that a foundation is laid for cloning of the final watermelon peripheral leaf shape ClLL gene and establishment of a molecular marker assisted breeding system, and a foundation is also laid for research of a watermelon leaf development regulation network.

The invention belongs to the technical field of plant engineering, and discloses an application of a lettuce LsSAW1 gene in controlling the heading character of lettuce, and the gene is shown as SEQ ID NO.1. The result of knocking out the LsSAW1 gene in the non-heading lettuce by virtue of a CRISPR-Cas9 technology shows that the LsSAW1 gene with lost functions can enable the non-heading lettuce to be heading. The LsSAW1 gene is transformed into the heading lettuce through agrobacterium tumefaciens mediation, and the result shows that the LsSAW1 can convert the heading lettuce into the non-heading lettuce. Cloning of the LsSAW1 gene opens up a new direction for research on plant leaf development and leaf dorsal ventral property, and meanwhile gene resources and theoretical guidance are provided for variety breeding of heading vegetables.

The invention discloses a medicago truncatula leaf development regulatory gene STF, and encoding protein of the gene. The medicago truncatula leaf development regulatory gene has a nucleotide sequence shown as SEQ ID No.2 and an amino acid sequence shown as SEQ ID No.1. An overexpression medicago truncatula leaf development regulatory gene STF can obviously improve two important agronomic characters, namely leaves and stems, of monocotyledon crops (rice), STF gene overexpression rice material leaves are widened and thickened, the stems are thick, the photosynthetic efficiency and lodging-resistant capacity of the rice are remarkably improved, and the problems that the photosynthetic efficiency is low, the stems are weak, lodging easily occurs and the like of the monocotyledon crops such as the rice in agricultural production can be solved.

The invention discloses a hedychium coronarium sesquiterpene synthetase gene HcTPS14 and application thereof. The full-length cDNA sequence of the HcTPS14 gene is shown in SEQ ID NO: 1; the coding sequence is shown in SEQ ID NO:2; and the coded amino acid sequence is shown in SEQ ID NO: 3. The HcTPS14 gene has certain expression in both leaf tissues and rhizomes; however, the HcTPS14 gene is hardly expressed in floral organs. Moreover, the expression level of the HcTPS14 gene is regulated by leaf development. Being used for catalyzing substrates, exogenous recombinant proteins with the HcTPS14gene allow generation of a medicinal sesquiterpene component, namely alpha-caryophyllene; and thus, the HcTPS14 gene can be used for preparing alpha-caryophyllene, and further into essential oil, essence and pharmaceuticals. Being connected with plant transformation vectors, and then introduced into cells of hedychium coronarium or other plants, the HcTPS14 gene allows obtaining of transgenic plants expressing the HcTPS14 gene. Moreover, specific molecular markers can be produced on basis of the gene sequence information disclosed by the invention so as to be used for identifying sesquiterpene-alpha-caryophyllene synthetase genes of hedychium coronarium and other plants, thereby realizing molecular-marker-assisted selected breeding so as to improve selection efficiency of breeding. Thus,relatively great application prospects are ensured.

The invention provides a Tanacetum cinerariifolium dwarf potting method. The Tanacetum cinerariifolium dwarf potting method includes the following steps: selecting healthy and full Tanacetum cinerariifolium seeds, and cultivating seedlings by using hole plates; performing potting while the seedlings have 1-2 pairs of real leaves, spraying leaves of the potted seedlings having 2 pairs of real leaves with a 80-230 mg/L daminozide solution, performing spraying once every 6-8 days, and totally performing spraying for 3-5 times. The Tanacetum cinerariifolium plants processed by the method is lowered to about 30% of contrast plants, and adverse impact on leaf development, plant width, and flower number of the Tanacetum cinerariifolium plants is less; the plant width of the Tanacetum cinerariifolium plants is above 75% of the contrast plants, the flower number of the Tanacetum cinerariifolium plants is above 80% of the contrast plants; the processed Tanacetum cinerariifolium plants are short,strong and compact, are sufficient in flowers, and are suitable for potting; and the diversity of natural plant-derived insect-resistant ornamental plants is enriched.

The invention belongs to the technical field of Chilades pandava prevention in a leaf development period of Cycas revolute and particularly relates to a Chilades pandava biological-physical prevention method applied to the leaf development period of Cycas revolute. According to the Chilades pandava biological-physical prevention method, young leaves of Cycas revolute are covered with a light transmitting and ventilating shield in the preliminary stage of leaf development of Chilades pandava, so that the young leaves are isolated from Chilades pandava adults, and the Chilades pandava adults are prevented from laying eggs on the young leaves of Cycas revolute. The Chilades pandava biological-physical prevention method is completely different from a traditional Cycas revolute insect prevention method, no or few chemicals are sprayed, and therefore, the purpose of preventing the Chilades pandava from gnawing the young leaves of Cycas revolute in the leaf development period is achieved, and integrity and ornamental value of the young leaves of Cycas revolute are ensured easily. By means of the Chilades pandava biological-physical prevention method, workload is small, pollution to the environment is avoided, species are not affected, and eco-environment protection is facilitated.

The invention belongs to the technical field of medical pueraria lobata planting, and particularly relates to a planting method for improving the content of total flavone in pueraria lobata. The planting method comprises seedling bed soil preparation and pre-picking management. Compared with the prior art, the planting method for improving the content of the total flavone in the pueraria lobata has the advantages that the seedling bed soil in the seedling stage and the management in the planting stage are adjusted appropriately, the nutritional growth and leaf development can be effectively promoted, and the planting method is helpful for the synthesis of flavone; compared with an existing planting technology, the content of the total flavone in the pueraria lobata can be improved while the yield is ensured, thereby conforming to the stipulation of the Chinese pharmacopoeia.