10 results about "Leaf development" patented technology

This invention provides a method for selecting plants that possess desired traits regarding the internode length of the main stem. [Solution] One aspect of the present invention is a selection method that includes a step of selecting plants using the length of the young shoot at the third leaf development stage as an indicator.

Leaf development, especially in leafy greens, is crucial for crop productivity. In various plants, DA1, a pivotal ubiquitin receptor that negatively regulates leaf growth, and EOD1 (ENHANCER1 OF DA1), an E3 ubiquitin ligases that interacts with DA1 through its RING domain, influence organ size. In this disclosure, in lettuce (Lactuca sativa), homologs genes were identified for both DA1 and EOD1, and da1 single mutants, da1 / eod1 double mutants, and DA1 overexpressing lines (DA1ox) were created. The da1 / eod1 double mutant exhibited a striking phenotype with larger organ size, accelerated growth, and increased biomass, resulting in higher yields and rapid growth traits pivotal for Controlled Environment Agriculture (CEA). Additionally, the da1 / eod1 double mutant displayed a pronounced ‘stay-green’ phenotype, characterized by elevated chlorophyll b levels, which implies extended shelf life, and enhanced postharvest quality, both traits indispensable for leafy vegetables in CEA settings. In contrast, the DA1ox line exhibited early senescence and diminished organ size. Based on these findings that spotlight the nuanced roles of the DA1-EOD1 module in modulating plant growth phases and its potential to revolutionize breeding strategies for improved yield and postharvest longevity in CEA-cultivated leafy greens, this disclosure provides guide RNAs for gene editing of DA1 and EOD1, and transformed lettuce having improved organ size and postharvest quality.

The invention relates to the technical field of biology, in particular to application of ZmMAPKKK70 protein and a coding gene thereof in regulation and control of corn phosphorus utilization, the ZmMAPKKK70 gene is screened out through whole genome correlation analysis, the ZmMAPKKK70 gene is knocked out from corn through a gene editing technology, and an obtained corn mutant material has the advantages that the corn mutant material can be used for regulating and controlling the phosphorus utilization of the corn in a field with phosphate fertilizer or without phosphate fertilizer; the Pseudomonas aeruginosa has the advantages in a plurality of growth indexes, including stability of leaf development, increase of plant height and stem diameter and improvement of final biomass and yield, and especially shows relatively strong adaptive capacity and growth potential under different phosphate fertilizer supply conditions. The corn ZmMAPKKK70 is verified to negatively regulate plant phosphorus utilization and overground biomass, plants which are more tolerant to a low-phosphorus environment and larger in overground biomass are obtained, gene resources are provided for new variety cultivation, and mutant materials have wide agricultural application potential, are particularly suitable for farmland environments with limited resources and insufficient phosphate fertilizer supply and have broad application prospects. Meanwhile, the obvious high-yield potential is also shown under the condition that the phosphate fertilizer is sufficient.

The invention discloses an Anemonium aureum SkORC3 gene as well as an expression protein and application thereof, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the SkORC3 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the SkORC3 gene is as shown in SEQ ID NO. 2. According to the invention, the gene is constructed into an overexpression vector and is converted into arabidopsis thaliana, so that the key function of the SkORC3 in regulating and controlling the morphogenesis of plant leaves is disclosed for the first time. A transgenic arabidopsis thaliana plant over-expressing the gene shows remarkable rosette leaf dysplasia, and the specific phenotypes comprise double main vein bifurcated heart-shaped leaves, symmetrical twin leaves at the top end of a petiole, uniaxial special-shaped leaves and the like. The invention provides a new gene resource and tool for molecular mechanism research of plant leaf development, and has potential application value in the field of ornamental plant leaf type molecular breeding.

The invention discloses application of casein kinase in regulation and control of flowering and leaf development of medicago sativa, and belongs to the technical field of biological genetic engineering. The multifunctionality of MsPPK3 as a key regulatory factor in coordination of vegetative growth and reproductive growth balance of medicago sativa is clarified for the first time, and specifically, MsPPK3 positively regulates plant reproductive growth and negatively regulates plant vegetative growth. Wherein the positive regulation and control of the reproductive growth of the plant is embodied in overexpression early flowering and silence late flowering. The negative regulation and control of plant vegetative growth is characterized in that the overexpressed plant is in a fine stem scattered form, and leaves are miniaturized, thinned and / or weakened form; the silent plants present a thick stem cluster type, a large leaf size, a thick leaf and / or a strengthened leaf. The method has great significance in the aspects of cultivation of different new varieties / new strains of alfalfa and the like.

This invention belongs to the field of molecular biology, and particularly relates to the application of the GhLEC1-1A gene and its encoded protein in cotton leaf development. The amino acid sequence of the encoded protein is shown in SEQ ID NO.2. This invention successfully introduced the GhLEC1-1A overexpression vector into plants and screened out positive overexpression plants. Compared with wild-type plants, the leaf growth and development morphology of the positive overexpression plants differed from that of the wild type, exhibiting increased leaf area and a final leaf shape resembling a chicken-foot leaf. These morphological changes provide important gene targets and germplasm resources for cotton molecular breeding, and offer technical support for the widespread application of the chicken-foot leaf technique in cotton production.

The application discloses application of BnWUS in regulating cell differentiation and leaf shape development of Brassica napus. The DNA nucleotide sequence of the BnWUS gene is shown as SEQ ID NO. 1, the sequence length is 951 bp, and the encoded amino acid sequence is shown as SEQ ID NO. 2. Functional analysis of Brassica napus genetic transformation shows that BnWUS is involved in regulating cell differentiation and leaf shape development of Brassica napus, overexpression of BnWUS can realize regulation of the leaf shape of Brassica napus, and the yield per plant is improved, thereby providing a theoretical basis for plant type improvement breeding of Brassica napus.

The application provides a compound microbial agent for promoting growth of tobacco plants and application thereof. The compound microbial agent comprises Bacillus altitudinis ZXY01, Bacillus subtilis LGT-2 and Rhodopseudomonas palustris. The compound microbial agent of the application comprises Bacillus altitudinis ZXY01 which can effectively promote release of potassium ions, help to improve the potassium nutrient level of tobacco plants, and provide support for stem robustness and material transportation; Rhodopseudomonas palustris has nitrogen fixation effect, can increase nitrogen source supply, and provide material basis for leaf development; Bacillus subtilis LGT-2 can promote root development, improve nutrient absorption efficiency, synergistically improve growth vigor of tobacco plants, increase environmental tolerance of the whole microbial group, and regulate the growth rate of the other two strains through metabolic products, thereby avoiding the problem of over-proliferation of a single strain due to the absence of Bacillus subtilis, and enabling the three strains to form a stable microbial group in the rhizosphere.

This invention discloses a MsMYB-ZD The application of genes in regulating alfalfa leaf size and yield falls under the field of plant genetic engineering. This invention utilizes transcriptome analysis to clone a gene that regulates alfalfa leaf size. MsMYB-ZD Transcription factors; experimental data show that, MsMYB-ZD The gene exhibits a gradually upregulated expression pattern during leaf development; compared to wild-type alfalfa Gongnong 1 (1-16) individual plants, overexpression... MsMYB-ZD MsMYB-ZD The leaf area of ​​the transgenic plants increased by an average of 77%, and the expression level of the transgenic lines showed a significant positive correlation with the leaf area. The fresh grass yield of the transgenic plants increased by 158.7%, and the dry grass yield increased by 207.5%. These results indicate that this gene is of great significance for improving alfalfa yield.

The invention discloses an application of a carya illinoensis CiSWEET10a gene and a promoter of the carya illinoensis CiSWEET10a gene in regulation and control of nut development and kernel filling, and belongs to the technical field of plant genetic engineering and biology. According to the invention, the CiSWEET10a gene is obtained through comparative analysis of transcriptomes and metabolomes of to-be-dropped young fruits and normally-developed young fruits by mining. The CiSWEET10a protein unloads cane sugar at the bottom of the carya illinoensis green seedcase, and the transported cane sugar is transported from seed coats to endosperm and embryos, so that the filling of seed kernels is promoted, and the development of nuts is guaranteed. The overexpressed CiSWEET10a in the transgenic plant promotes the transportation of cane sugar from seed coats to endosperm and embryos, increases the content of starch in the plant, and increases the quality of a single fruit and the quality of a single spike. The CiSWEET10a gene promoter regulates and controls leaf development, root system and lateral root development, male flower and female flower development and fruit development, and has multiple physiological functions. The invention has important theoretical significance and practical significance for further explaining the molecular mechanism of plant grain development and cultivating new varieties of high-quality and high-yield crops through genetic engineering means.