LX-88 strain with good selenium conversion and Se (0) oxidation capacities and application of LX-88 strain

A technology of LX-88 and selenium bacteria agent, which is applied in the field of LX-88 bacteria, can solve the problems that there is no extensive research on the application, and achieve the effect of promoting selenium enrichment in plants

Active Publication Date: 2021-08-31
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first type strain of this genus was reported by Liu et al. in 2019, and its application has not been extensively studied

Method used

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  • LX-88 strain with good selenium conversion and Se (0) oxidation capacities and application of LX-88 strain
  • LX-88 strain with good selenium conversion and Se (0) oxidation capacities and application of LX-88 strain
  • LX-88 strain with good selenium conversion and Se (0) oxidation capacities and application of LX-88 strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation and Identification of Strain LX-88

[0045] (1) Sample collection: The applicant collected the selenium-rich soil samples used in the present invention from the surface soil in the selenium-rich area of ​​Enshi Tujia and Miao Autonomous Prefecture, Hubei Province in October 2018.

[0046] (2) Separation and purification of bacteria: Weigh 10g of the soil sample into a conical flask filled with 90mL of sterile normal saline, place it in a shaker at 28°C for half an hour, then take 1mL and add it to 9mL of sterile normal saline to gradually dilute to 10 -1 , 10 -2 , 10 -3 , 10 -4 , respectively take 0.1mL and spread in 0.5mM Se(IV)R2A solid medium, and coat 3 plates for each gradient. Incubate at a constant temperature of 28°C for one week, pick a single white colony and streak for purification. After purification, store it in a screw tube with 50% glycerol:bacteria solution (volume ratio 1:1), and store it at -80°C.

[0047] (3) Screening of selenium tran...

Embodiment 2

[0057] Transformation utilization curve of strain LX-88 to SeMet:

[0058] The specific steps are as follows:

[0059] (1) Prepare 3 bottles of sterilized R2A liquid medium, 50mL in each bottle. Filter-sterilized SeMet was added to each at a final concentration. Inoculation log phase (OD 600 The value is about 0.8) LX-88 bacterial liquid, the inoculum size is 1%. Immediately after mixing, take a 2mL sample, which is taken as the first sample, and placed in a clean centrifuge tube. Placed in a 28°C, 150rpm constant temperature shaker for cultivation. Samples were taken every 12 hours, and the experiment was repeated three times. The R2A liquid medium with no strain LX-88 and only SeMet with the same final concentration was used as a control; after sampling, the samples were treated as follows.

[0060] (2) 2 mL of samples were taken each time, centrifuged at 12000 rpm for 10 min, and the supernatant was passed through a 0.22 μm filter membrane and stored in a -20°C refrige...

Embodiment 3

[0066] Oxidation Curve of Elemental Selenium by Strain LX-88

[0067] The specific steps are as follows:

[0068] (1) Prepare 3 bottles of sterilized R2A liquid medium, 50mL in each bottle. Each added to a final concentration of 0.1g L -1 Sterilized elemental selenium powder. Inoculation log phase (OD 600 The value is about 0.8) LX-88 bacterial liquid, the inoculum size is 1%. Immediately after mixing, take a 2mL sample, which is taken as the first sample, and placed in a clean centrifuge tube. Placed in a 28°C, 150rpm constant temperature shaker for cultivation. Samples were taken every 12 hours, and the experiment was repeated three times. The R2A liquid culture medium with no addition of strain LX-88 and only the same final concentration of elemental selenium was used as a control; after sampling, the samples were treated as follows.

[0069] (2) 2 mL of samples were taken each time, centrifuged at 12000 rpm for 10 min, and the supernatant was passed through a 0.22 μm...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and particularly discloses an LX-88 strain with good selenium conversion and Se (0) oxidation capacity and application of the LX-88 strain, and the preservation number of the strain is CCTCC NO: M2021542. The strain can convert SeMet into selenocystine (SeCys2), methyl selenocysteine (MSeCys) and the like and has an oxidation effect on elemental selenium, and the strain LX-88 can be used for reducing Se (IV) and can be used for reducing the Se (IV) into red nano-selenium (SeNPs), SeCys2 and MSeCys. Therefore, the strain LX-88 can be used as an industrial microorganism and is applied to production and preparation of SeCys2 and MSeCys; the strain can be used as a model microorganism for research on metabolic transformation of organic selenium and Se (IV); the plant selenium-rich microbial inoculum can be prepared and can be used as a microorganism for Se (0) oxidation research.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and relates to a LX-88 bacterium with good selenium conversion and Se(0) oxidation ability and application thereof. The bacterial strain is Croceibacteriumsp.LX-88. Background technique [0002] Selenium (Se) is located in the main group VI of the periodic table of elements. Selenium exists in four valence states of Se(-II), Se(0), Se(IV) and Se(VI) in nature. Se(-II) is mainly organic selenium, which is commonly found in organisms, such as selenocysteine ​​(SeCys), selenomethionine (SeMet), monomethylselenium (CH 3 -Se), dimethylselenium (CH 3 -Se-CH 3 ) and methylselenocysteine ​​(SeMeCys), etc. Se(IV) and Se(VI) are mainly inorganic selenium, mostly present in non-biological organisms, usually as oxyanions, such as selenite (SeO 3 2- ) and selenate (SeO 4 2- ), is the main source of selenium absorbed and utilized by plants. The chemical element selenium Se(0) is rare in n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P9/00C12R1/01
CPCC12P9/00
Inventor 李明顺郑世学王革娇罗雄蓝艳
Owner HUAZHONG AGRI UNIV
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