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A lx-88 strain with good selenium transformation and se(0) oxidation ability and its application

A technology of selenium bacteria agent and selenium oxide, which is applied in the field of LX-88 bacteria, can solve the problems that there is no extensive research on the application, and achieve the effect of promoting selenium enrichment in plants

Active Publication Date: 2022-05-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first type strain of this genus was reported by Liu et al. in 2019, and its application has not been extensively studied

Method used

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  • A lx-88 strain with good selenium transformation and se(0) oxidation ability and its application
  • A lx-88 strain with good selenium transformation and se(0) oxidation ability and its application
  • A lx-88 strain with good selenium transformation and se(0) oxidation ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Isolation and Identification of Strain LX-88

[0045] (1) Sample collection: The applicant collected the selenium-rich soil samples used in the present invention from the surface soil in the selenium-rich area of ​​Enshi Tujia and Miao Autonomous Prefecture, Hubei Province in October 2018.

[0046] (2) Separation and purification of bacteria: Weigh 10g of the soil sample into a conical flask filled with 90mL of sterile normal saline, place it in a shaker at 28°C for half an hour, then take 1mL and add it to 9mL of sterile normal saline to gradually dilute to 10 -1 , 10 -2 , 10 -3 , 10 -4 , respectively take 0.1mL and spread in 0.5mM Se(IV)R2A solid medium, and coat 3 plates for each gradient. Incubate at a constant temperature of 28°C for one week, pick a single white colony and streak for purification. After purification, store it in a screw tube with 50% glycerol:bacteria solution (volume ratio 1:1), and store it at -80°C.

[0047] (3) Screening of selenium tran...

Embodiment 2

[0057] Transformation and utilization curve of SeMet by strain LX-88:

[0058] The specific steps are as follows:

[0059] (1) Prepare 3 bottles of sterilized R2A liquid medium, 50mL in each bottle. Filter-sterilized SeMet was added to each at a final concentration. Inoculation log phase (OD 600 The value is about 0.8) LX-88 bacterial liquid, the inoculum size is 1%. Immediately after mixing, sample 2 mL, which is taken as the first sample, and placed in a clean centrifuge tube. Placed in a constant temperature shaker at 28°C and 150rpm. Samples were taken every 12 hours, and the experiment was repeated three times. The R2A liquid medium with no strain LX-88 and only SeMet with the same final concentration was used as a control; after sampling, the samples were treated as follows.

[0060] (2) 2 mL of samples were taken each time, centrifuged at 12000 rpm for 10 min, and the supernatant was passed through a 0.22 μm filter membrane and stored in a -20°C refrigerator.

[0...

Embodiment 3

[0066] Oxidation Curve of Elemental Selenium by Strain LX-88

[0067] The specific steps are as follows:

[0068] (1) Prepare 3 bottles of sterilized R2A liquid medium, 50mL in each bottle. Each added to a final concentration of 0.1g L -1 Sterilized elemental selenium powder. Inoculation log phase (OD 600 The value is about 0.8) LX-88 bacterial liquid, the inoculum size is 1%. Immediately after mixing, take a 2mL sample, which is taken as the first sample, and placed in a clean centrifuge tube. Placed in a 28°C, 150rpm constant temperature shaker for cultivation. Samples were taken every 12 hours, and the experiment was repeated three times. The R2A liquid culture medium with the same final concentration of elemental selenium was used as the control without adding the strain LX-88; after the sampling was finished, the samples were treated as follows.

[0069] (2) 2 mL of samples were taken each time, centrifuged at 12000 rpm for 10 min, and the supernatant was passed thr...

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Abstract

The invention belongs to the technical field of agricultural microorganisms, and specifically discloses a LX-88 bacterium with good selenium conversion and Se(0) oxidation capabilities and its application. The preservation number of the strain is CCTCC NO: M2021542. The strain can convert SeMet into selenocysteine ​​(SeCys2) and methylselenocysteine ​​(MSeCys), etc., and has oxidation effect on elemental selenium. The strain LX‑88 can reduce Se(IV), and can convert Se (IV) Reduction to red nanoselenium (SeNPs), SeCys2 and MSeCys. Therefore, the bacterial strain LX‑88 can be used as an industrial microorganism for the production and preparation of SeCys2 and MSeCys; it can be used as a model microorganism for the study of organic selenium and Se(IV) metabolic transformation; Microorganisms for the study of oxidation.

Description

technical field [0001] The invention belongs to the technical field of agricultural microbes, and relates to a LX-88 bacterium with good selenium conversion and Se(0) oxidation ability and application thereof. The bacterial strain is Croceibacteriumsp.LX-88. Background technique [0002] Selenium (Se) is located in the main group VI of the periodic table of elements. Selenium exists in four valence states of Se(-II), Se(0), Se(IV) and Se(VI) in nature. Se(-II) is mainly organic selenium, which is commonly found in organisms, such as selenocysteine ​​(SeCys), selenomethionine (SeMet), monomethylselenium (CH 3 -Se), dimethylselenium (CH 3 -Se-CH 3 ) and methylselenocysteine ​​(SeMeCys), etc. Se(IV) and Se(VI) are mainly inorganic selenium, mostly present in non-biological organisms, usually as oxyanions, such as selenite (SeO 3 2- ) and selenate (SeO 4 2- ), is the main source of selenium absorbed and utilized by plants. The chemical element selenium Se(0) is rare in n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P9/00C12R1/01
CPCC12P9/00
Inventor 李明顺郑世学王革娇罗雄蓝艳
Owner HUAZHONG AGRI UNIV
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